Standard name | |
---|---|
Human Ortholog | |
Description | Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 29 | 38 | 55 | 59 | 76 | 31 | 47 | 22 | 19 | 23 | 43 | 2 | 2 | 7 | 24 | 8 | 12 | 2 | 12 | 27 | 2 | 6 | 12 |
Bud | – | 2 | 3 | 7 | 27 | 37 | 26 | 25 | 33 | 30 | 29 | 36 | 0 | 0 | 2 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 2 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 6 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 6 | 3 | 5 | 2 | 5 | 4 | 5 | 5 | 6 | 4 | 7 | 0 | 0 | 1 | 4 | 10 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 133 | 114 | 56 | 45 | 68 | 66 | 52 | 33 | 46 | 24 | 52 | 25 | 46 | 61 | 148 | 120 | 118 | 4 | 101 | 80 | 17 | 27 | 47 |
Endoplasmic Reticulum | – | 40 | 49 | 46 | 52 | 91 | 52 | 66 | 30 | 26 | 13 | 44 | 7 | 14 | 11 | 9 | 6 | 3 | 3 | 31 | 29 | 13 | 14 | 40 |
Endosome | – | 2 | 18 | 14 | 46 | 27 | 21 | 26 | 26 | 25 | 17 | 14 | 0 | 1 | 3 | 9 | 6 | 5 | 3 | 9 | 24 | 9 | 6 | 2 |
Golgi | – | 31 | 27 | 94 | 119 | 62 | 28 | 39 | 27 | 33 | 11 | 20 | 0 | 4 | 3 | 21 | 16 | 10 | 3 | 53 | 68 | 12 | 12 | 18 |
Mitochondria | – | 2 | 35 | 69 | 164 | 293 | 395 | 374 | 318 | 347 | 264 | 391 | 1 | 1 | 0 | 12 | 4 | 6 | 0 | 9 | 16 | 6 | 4 | 6 |
Nucleus | – | 2 | 0 | 0 | 0 | 1 | 1 | 5 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | 1 | 4 | 3 | 0 | 3 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 1 | 2 | 1 | 4 | 27 | 5 | 3 | 6 | 7 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 1 | 4 | 3 | 3 | 3 | 5 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | – | 0 | 0 | 1 | 1 | 6 | 2 | 8 | 6 | 14 | 7 | 9 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 1 | 2 | 1 | 5 | 1 | 10 | 20 | 8 | 16 | 4 | 3 | 0 | 3 | 0 | 11 | 1 | 14 | 0 | 6 | 4 | 5 | 4 | 9 |
Unique Cell Count | 204 | 224 | 239 | 359 | 488 | 512 | 545 | 407 | 471 | 320 | 487 | 34 | 68 | 80 | 206 | 156 | 155 | 21 | 234 | 261 | 69 | 84 | 150 | |
Labelled Cell Count | 248 | 289 | 349 | 523 | 673 | 648 | 701 | 520 | 580 | 410 | 635 | 35 | 75 | 89 | 244 | 173 | 177 | 21 | 234 | 261 | 69 | 84 | 150 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 13.6 | 9.2 | 8.9 | 8.2 | 7.6 | 6.6 | 7.0 | 6.8 | 6.5 | 6.3 | 6.3 | 11.0 | 10.2 | 12.3 | 13.5 | 14.1 | 13.8 | 13.2 | 14.2 | 14.7 |
Std Deviation (1e-4) | – | 2.1 | 1.4 | 1.5 | 1.2 | 1.4 | 1.3 | 1.4 | 1.4 | 1.3 | 1.3 | 1.2 | 1.9 | 2.2 | 1.9 | 2.4 | 2.6 | 2.6 | 2.6 | 2.6 | 3.4 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.16 | -0.27 | -0.48 | -0.38 | -0.42 | -0.49 | -0.53 | -0.55 | 0.27 | 0.16 | 0.43 | 0.56 | 0.62 | 0.59 | 0.53 | 0.63 | 0.69 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.6 | -0.2 | -0.5 | -4.7 | -3.4 | -4.7 | -5.8 | -3.6 | -3.2 | -1.7 | -2.9 | -1.8 | -1.6 | -3.5 | -2.6 |
Bud | 0 | 3.3 | 3.4 | 2.4 | 2.2 | 3.5 | 2.9 | 3.8 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 0 |
Cytoplasm | -6.1 | -10.1 | -10.5 | -11.0 | -12.7 | -12.2 | -12.0 | -11.4 | -11.8 | 2.5 | 2.4 | 3.9 | 4.4 | 5.1 | 5.0 |
Endoplasmic Reticulum | -0.7 | -2.3 | -1.0 | -4.3 | -3.4 | -5.3 | -6.5 | -6.4 | -4.7 | -0.2 | -0.2 | -1.6 | -5.3 | -4.9 | -5.5 |
Endosome | -0.9 | 1.8 | -1.3 | -2.2 | -1.8 | -0.8 | -1.4 | -1.3 | -3.1 | 0 | 0 | -1.3 | -1.6 | -1.7 | -1.9 |
Golgi | 6.7 | 5.7 | 0.2 | -3.1 | -2.2 | -2.3 | -2.2 | -3.9 | -4.0 | 0 | -1.4 | -2.1 | -0.6 | -0.5 | -1.8 |
Mitochondria | 3.4 | 7.4 | 11.0 | 15.6 | 13.4 | 15.1 | 14.4 | 15.4 | 16.3 | 0 | -3.1 | -3.8 | -3.3 | -4.1 | -3.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 2.1 | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 2.7 | 0 | 3.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.8892 | 6.9617 | 6.6074 | 5.8747 | 5.7304 | 6.5926 | 7.1795 | 8.91 | 7.7789 | 8.044 | 6.5447 | 7.9686 | 4.0154 | 5.0796 | 4.7177 | 2.7868 | 3.4392 | 4.8539 |
Actin | 0.0161 | 0.0108 | 0.027 | 0.0204 | 0.0106 | 0.0086 | 0.0694 | 0.0187 | 0.07 | 0.0118 | 0.0269 | 0.0398 | 0.0461 | 0.0632 | 0.0151 | 0.0038 | 0.0469 | 0.0163 |
Bud | 0.0008 | 0.0026 | 0.006 | 0.0006 | 0.0003 | 0.0021 | 0.001 | 0.0014 | 0.0008 | 0.001 | 0.0006 | 0.0005 | 0.0005 | 0.0011 | 0.0004 | 0.0008 | 0.0005 | 0.0005 |
Bud Neck | 0.0011 | 0.0006 | 0.0007 | 0.0005 | 0.0008 | 0.0018 | 0.003 | 0.0007 | 0.0009 | 0.0009 | 0.0006 | 0.0025 | 0.001 | 0.0007 | 0.0005 | 0.0008 | 0.001 | 0.0018 |
Bud Periphery | 0.0005 | 0.001 | 0.0016 | 0.0003 | 0.0003 | 0.0016 | 0.0006 | 0.0003 | 0.0003 | 0.0005 | 0.0004 | 0.0002 | 0.0003 | 0.0007 | 0.0001 | 0.0008 | 0.0006 | 0.0002 |
Bud Site | 0.0027 | 0.0116 | 0.0024 | 0.0011 | 0.0004 | 0.0018 | 0.0113 | 0.0102 | 0.0017 | 0.0011 | 0.0005 | 0.0005 | 0.0013 | 0.0033 | 0.0008 | 0.0056 | 0.0022 | 0.0003 |
Cell Periphery | 0.0005 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0001 | 0.001 | 0.0002 | 0.0001 |
Cytoplasm | 0.2523 | 0.3495 | 0.2006 | 0.0967 | 0.2222 | 0.244 | 0.1016 | 0.3003 | 0.0862 | 0.0953 | 0.0507 | 0.1352 | 0.0809 | 0.2201 | 0.1415 | 0.1228 | 0.0072 | 0.2589 |
Cytoplasmic Foci | 0.1384 | 0.1573 | 0.16 | 0.1695 | 0.1402 | 0.1384 | 0.1801 | 0.1792 | 0.171 | 0.1511 | 0.18 | 0.1538 | 0.1552 | 0.1882 | 0.1526 | 0.0912 | 0.2361 | 0.1174 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0008 | 0.0001 |
Endoplasmic Reticulum | 0.0231 | 0.0155 | 0.0053 | 0.0071 | 0.0082 | 0.0129 | 0.0108 | 0.0033 | 0.0054 | 0.0066 | 0.0038 | 0.0171 | 0.0204 | 0.0132 | 0.0086 | 0.02 | 0.0051 | 0.0249 |
Endosome | 0.3195 | 0.2789 | 0.3194 | 0.521 | 0.3605 | 0.3269 | 0.2434 | 0.2252 | 0.241 | 0.3867 | 0.3188 | 0.2374 | 0.2542 | 0.2185 | 0.2924 | 0.3062 | 0.1528 | 0.2508 |
Golgi | 0.2095 | 0.1443 | 0.2415 | 0.1305 | 0.2028 | 0.2224 | 0.3412 | 0.2473 | 0.4016 | 0.3013 | 0.4043 | 0.3909 | 0.4033 | 0.2715 | 0.3482 | 0.1949 | 0.3559 | 0.2932 |
Lipid Particles | 0.0038 | 0.0016 | 0.0012 | 0.0029 | 0.0176 | 0.0009 | 0.0069 | 0.0009 | 0.0017 | 0.0015 | 0.0013 | 0.0016 | 0.0086 | 0.0008 | 0.0008 | 0.0297 | 0.0946 | 0.0004 |
Mitochondria | 0.0144 | 0.0114 | 0.0128 | 0.0065 | 0.0155 | 0.0266 | 0.0066 | 0.0042 | 0.0075 | 0.0211 | 0.0047 | 0.0118 | 0.0064 | 0.0067 | 0.01 | 0.1432 | 0.0292 | 0.0175 |
None | 0.0039 | 0.0016 | 0.0039 | 0.0014 | 0.0019 | 0.0013 | 0.0065 | 0.0005 | 0.0004 | 0.0005 | 0.0004 | 0.0005 | 0.0011 | 0.0008 | 0.0189 | 0.0156 | 0.0013 | 0.0006 |
Nuclear Periphery | 0.0015 | 0.0012 | 0.0006 | 0.0009 | 0.0051 | 0.0006 | 0.002 | 0.0003 | 0.0019 | 0.0027 | 0.0003 | 0.0004 | 0.0048 | 0.0011 | 0.0014 | 0.0102 | 0.0006 | 0.0003 |
Nucleolus | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0018 | 0.0002 | 0 |
Nucleus | 0.0006 | 0.0008 | 0.0009 | 0.0003 | 0.0007 | 0.0005 | 0.0011 | 0.0002 | 0.0007 | 0.0005 | 0.0001 | 0.0002 | 0.0008 | 0.0004 | 0.001 | 0.0042 | 0.0002 | 0.0002 |
Peroxisomes | 0.0048 | 0.0044 | 0.0087 | 0.0298 | 0.0029 | 0.0026 | 0.0075 | 0.0028 | 0.006 | 0.0076 | 0.0027 | 0.0044 | 0.0082 | 0.0044 | 0.002 | 0.0079 | 0.06 | 0.0138 |
Punctate Nuclear | 0.0006 | 0.0004 | 0.0003 | 0.0005 | 0.001 | 0.0004 | 0.0037 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0024 | 0.0022 | 0.002 | 0.0001 |
Vacuole | 0.0034 | 0.0042 | 0.0038 | 0.0049 | 0.0044 | 0.003 | 0.0017 | 0.0028 | 0.0011 | 0.0051 | 0.002 | 0.0013 | 0.0038 | 0.0032 | 0.0014 | 0.0254 | 0.0011 | 0.001 |
Vacuole Periphery | 0.0023 | 0.0021 | 0.0028 | 0.0048 | 0.0041 | 0.0029 | 0.001 | 0.0012 | 0.0012 | 0.0044 | 0.0017 | 0.0015 | 0.0022 | 0.0016 | 0.0016 | 0.0118 | 0.0013 | 0.0016 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.358 | 18.2662 | 19.9748 | 27.778 | 34.859 | 33.3203 | 21.3851 | 25.9147 | 26.8833 | 27.3873 |
Translational Efficiency | 4.3409 | 3.1296 | 3.2822 | 2.3931 | 2.1269 | 2.5308 | 2.4553 | 2.5696 | 2.6492 | 2.7459 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1339 | 1239 | 1929 | 112 | 1490 | 1414 | 258 | 1318 | 2829 | 2653 | 2187 | 1430 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1148.53 | 1093.49 | 1267.27 | 1727.42 | 1035.90 | 969.46 | 1513.46 | 1450.93 | 1089.21 | 1027.38 | 1296.31 | 1472.59 |
Standard Deviation | 174.52 | 194.36 | 188.36 | 259.70 | 154.86 | 172.62 | 174.89 | 231.85 | 173.81 | 193.27 | 203.00 | 245.65 |
Intensity Change Log 2 | — | -0.070849 | 0.141935 | 0.588830 | — | -0.095631 | 0.546966 | 0.486093 | — | -0.082548 | 0.348207 | 0.541021 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.007241 | 0.005341 | 0.010876 | 0.014436 | 0.003176 | 0.004685 | 0.047842 | 0.045750 | 0.005100 | 0.004991 | 0.015237 | 0.043297 |
Bud Neck | 0.000265 | 0.000753 | 0.000556 | 0.004685 | 0.000317 | 0.000310 | 0.001750 | 0.001280 | 0.000292 | 0.000517 | 0.000697 | 0.001546 |
Bud Site | 0.018619 | 0.029356 | 0.014594 | 0.071883 | 0.016552 | 0.010044 | 0.046693 | 0.044097 | 0.017530 | 0.019063 | 0.018381 | 0.046273 |
Cell Periphery | 0.000382 | 0.000656 | 0.000250 | 0.002993 | 0.000453 | 0.000267 | 0.000696 | 0.005698 | 0.000419 | 0.000448 | 0.000303 | 0.005486 |
Cytoplasm | 0.040135 | 0.042109 | 0.014766 | 0.128678* | 0.032650 | 0.017681 | 0.008977 | 0.057330 | 0.036193 | 0.029089 | 0.014083 | 0.062918 |
Cytoplasmic Foci | 0.498093* | 0.422581* | 0.274182* | 0.218024* | 0.463509* | 0.342059* | 0.267926* | 0.240658* | 0.479878* | 0.379664* | 0.273444* | 0.238885* |
Eisosomes | 0.000093 | 0.000117 | 0.000079 | 0.000061 | 0.000114 | 0.000121 | 0.000202 | 0.000582 | 0.000104 | 0.000119 | 0.000093 | 0.000542 |
Endoplasmic Reticulum | 0.007373 | 0.005355 | 0.008021 | 0.002775 | 0.006354 | 0.003674 | 0.006537 | 0.008353 | 0.006836 | 0.004459 | 0.007846 | 0.007916 |
Endosome | 0.048382 | 0.087034 | 0.196511 | 0.094048 | 0.063259 | 0.158159 | 0.170292 | 0.104067 | 0.056218 | 0.124942 | 0.193418 | 0.103282 |
Golgi | 0.358560* | 0.370169* | 0.395433* | 0.245250* | 0.383979* | 0.422879* | 0.398880* | 0.422908* | 0.371948* | 0.398263* | 0.395839* | 0.408994* |
Lipid Particles | 0.002305 | 0.007038 | 0.023115 | 0.071078 | 0.004768 | 0.011750 | 0.016782 | 0.042197 | 0.003602 | 0.009550 | 0.022368 | 0.044459 |
Mitochondria | 0.001863 | 0.004328 | 0.001167 | 0.000079 | 0.003894 | 0.004455 | 0.000122 | 0.002205 | 0.002933 | 0.004396 | 0.001044 | 0.002039 |
Mitotic Spindle | 0.001695 | 0.003234 | 0.012626 | 0.041982 | 0.001581 | 0.002547 | 0.003511 | 0.001651 | 0.001635 | 0.002867 | 0.011551 | 0.004810 |
None | 0.000056 | 0.000525 | 0.000032 | 0.000065 | 0.000065 | 0.000136 | 0.000009 | 0.000069 | 0.000061 | 0.000317 | 0.000029 | 0.000069 |
Nuclear Periphery | 0.000018 | 0.000019 | 0.000166 | 0.000103 | 0.000025 | 0.000035 | 0.000035 | 0.000021 | 0.000021 | 0.000028 | 0.000150 | 0.000027 |
Nuclear Periphery Foci | 0.000774 | 0.000602 | 0.003991 | 0.007306 | 0.000641 | 0.001036 | 0.001412 | 0.000504 | 0.000704 | 0.000834 | 0.003687 | 0.001036 |
Nucleolus | 0.000006 | 0.000035 | 0.000029 | 0.000029 | 0.000008 | 0.000047 | 0.000002 | 0.000008 | 0.000007 | 0.000041 | 0.000026 | 0.000010 |
Nucleus | 0.000151 | 0.000277 | 0.000109 | 0.000608 | 0.000099 | 0.000091 | 0.000037 | 0.000134 | 0.000124 | 0.000177 | 0.000100 | 0.000171 |
Peroxisomes | 0.000982 | 0.001460 | 0.001110 | 0.004272 | 0.000954 | 0.002104 | 0.003933 | 0.006616 | 0.000967 | 0.001803 | 0.001443 | 0.006432 |
Vacuole | 0.003551 | 0.008622 | 0.033860 | 0.083075 | 0.004770 | 0.007782 | 0.021651 | 0.010726 | 0.004193 | 0.008174 | 0.032420 | 0.016392 |
Vacuole Periphery | 0.009457 | 0.010389 | 0.008527 | 0.008571 | 0.012832 | 0.010138 | 0.002710 | 0.005147 | 0.011235 | 0.010255 | 0.007841 | 0.005416 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.43 | -2.03 | -1.76 | -2.33 | -0.79 | -1.86 | -5.48 | -12.38 | -11.80 | 0.24 | 0.13 | -6.04 | -11.85 | -11.95 | -7.92 |
Bud Neck | -3.63 | -4.88 | -5.77 | -5.07 | -5.39 | 0.08 | -5.04 | -7.84 | -8.02 | 1.51 | -3.34 | -6.92 | -9.75 | -7.33 | -6.25 |
Bud Site | -4.01 | 2.43 | -4.66 | -3.63 | -5.00 | 4.54 | -4.73 | -9.87 | -12.69 | 0.34 | -1.10 | -0.70 | -10.89 | -9.70 | -9.89 |
Cell Periphery | -2.43 | 2.13 | -2.82 | -2.49 | -2.98 | 1.74 | -2.01 | -6.18 | -6.40 | -5.88 | -0.42 | 2.17 | -6.45 | -6.40 | -6.61 |
Cytoplasm | -0.58 | 8.64 | -4.52 | -4.40 | -5.70 | 5.59 | 9.33 | -5.73 | -9.64 | -11.95 | 2.90 | 10.82 | -6.50 | -8.10 | -12.04 |
Cytoplasmic Foci | 9.43 | 33.48 | 19.15 | 13.98 | 4.40 | 17.03 | 16.68 | 32.11 | 15.74 | 2.43 | 18.56 | 39.30 | 42.21 | 24.88 | 6.41 |
Eisosomes | -1.62 | 1.19 | 2.24 | 3.70 | 1.45 | -0.35 | -2.16 | -7.79 | -7.72 | -5.46 | -1.21 | 0.94 | -7.99 | -7.73 | -8.22 |
Endoplasmic Reticulum | 1.91 | -0.54 | 4.07 | 2.80 | 4.54 | 2.57 | -0.11 | -1.67 | -4.14 | -1.07 | 3.20 | -1.03 | -1.05 | -3.63 | -0.02 |
Endosome | -12.04 | -33.50* | -5.11 | -0.32 | 11.88 | -21.13 | -13.80 | -12.32 | 11.11 | 8.32 | -23.63 | -34.35* | -16.42 | 6.00 | 19.74 |
Golgi | -1.27 | -4.21 | 5.77 | 6.23 | 7.34 | -4.67 | -0.89 | -4.05 | 0.19 | -1.42 | -4.30 | -3.25 | -4.26 | -0.99 | -1.28 |
Lipid Particles | -5.15 | -12.77 | -4.74 | -4.42 | -3.35 | -4.12 | -4.32 | -12.46 | -9.42 | -6.42 | -5.96 | -12.27 | -13.92 | -11.53 | -6.86 |
Mitochondria | -2.27 | 1.84 | 8.36 | 3.99 | 3.50 | -0.63 | 7.41 | 2.08 | 2.30 | -3.21 | -2.10 | 4.78 | 1.37 | 2.72 | -1.52 |
Mitotic Spindle | -2.15 | -8.00 | -3.21 | -3.10 | -2.47 | -1.55 | -2.58 | -0.30 | 1.28 | 2.33 | -2.61 | -8.27 | -3.00 | -1.91 | 3.54 |
None | -2.30 | 3.35 | -0.51 | 2.23 | -1.48 | -1.16 | 5.25 | -0.24 | 1.07 | -3.86 | -2.55 | 4.38 | -0.55 | 2.45 | -2.66 |
Nuclear Periphery | -0.54 | -6.13 | -4.59 | -4.48 | 1.26 | -1.14 | -1.62 | 0.53 | 1.74 | 3.03 | -1.28 | -6.05 | -1.96 | -0.28 | 5.68 |
Nuclear Periphery Foci | 0.40 | -4.83 | -4.30 | -4.51 | -2.37 | -1.10 | -2.67 | 0.59 | 1.60 | 3.77 | -0.49 | -5.76 | -1.65 | -1.29 | 5.15 |
Nucleolus | -2.07 | -2.66 | -2.42 | -0.53 | -0.84 | -1.37 | 2.23 | -0.37 | 1.34 | -4.33 | -2.05 | -2.51 | -1.94 | 1.77 | 1.91 |
Nucleus | -1.27 | 0.56 | -2.16 | -1.56 | -2.36 | 0.21 | 2.68 | -1.12 | -1.26 | -2.97 | -1.13 | 0.48 | -1.33 | -0.07 | -1.93 |
Peroxisomes | -2.84 | -0.90 | -3.06 | -2.58 | -2.92 | -3.98 | -4.38 | -9.62 | -6.95 | -2.99 | -4.82 | -3.36 | -9.98 | -8.13 | -8.92 |
Vacuole | -4.64 | -16.04 | -6.93 | -6.50 | -4.42 | -2.95 | -5.05 | -5.28 | -2.62 | 2.98 | -5.35 | -16.43 | -8.58 | -5.67 | 6.69 |
Vacuole Periphery | -0.83 | 1.03 | 0.92 | 1.47 | 0.30 | 1.99 | 8.67 | 6.60 | 5.69 | -4.60 | 1.11 | 4.06 | 7.71 | 7.10 | 3.98 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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