Standard name
Human Ortholog
Description Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; functions as a chaperone under ATP-depleted oxidative stress conditions; subunit of GET complex, involved in ATP dependent Golgi to ER trafficking and insertion of tail-anchored (TA) proteins into ER membrane under non-stress conditions; binds as dimer to transmembrane domain (TMD) cargo, shielding TMDs from aqueous solvent; protein abundance increases under DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.07 0.05 0.07 0.05 0 0.06 0.07 0.1 0.09 0.06 0 0.06 0.07 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0.87 0.87 0.82 0.86 0.83 0.8 0.73 0.66 0.71 0.67 0.7 0.67 0.94 0.99 0.98 0.88 0.95 0.92 0.44 0.74 0.65 0.78 0.74 0.76
Endoplasmic Reticulum 0.14 0.17 0.22 0.13 0.16 0.14 0.1 0.15 0.12 0.15 0.12 0.18 0.07 0 0 0 0 0 0.51 0.23 0.27 0.17 0.15 0.14
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0.06 0.17 0.18 0.13 0.14 0.08 0.07 0 0 0 0.1 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 4 5 4 8 6 7 0 0 0 0 0 0 2 0 0 0 3 3
Bud 1 1 0 1 0 0 2 0 2 3 4 2 1 1 0 0 1 0 0 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 4 13 18 26 29 23 18 21 31 31 24 14 7 18 28 12 22 0 0 0 1 3 0
Cytoplasm 194 188 259 232 397 341 365 286 265 304 225 179 222 280 315 328 202 385 104 152 227 249 293 303
Endoplasmic Reticulum 32 36 68 35 75 61 51 66 44 68 38 49 16 8 10 5 2 7 120 47 95 54 58 54
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 4 5
Golgi 0 0 1 0 0 1 2 0 1 0 0 0 0 0 1 3 0 0 0 0 3 0 6 9
Mitochondria 1 1 0 0 0 2 9 4 15 12 23 8 0 0 0 0 0 2 0 0 1 2 3 1
Nucleus 2 0 1 1 0 1 4 6 1 10 4 3 5 3 1 3 0 1 0 0 1 0 4 1
Nuclear Periphery 0 0 0 0 0 2 0 2 0 0 0 0 0 0 0 0 0 0 3 0 3 0 2 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Vac/Vac Membrane 1 0 6 6 25 27 85 76 48 62 25 20 0 1 0 37 7 23 2 2 6 2 8 4
Unique Cell Count 223 215 316 270 478 427 503 431 375 451 321 267 236 284 322 374 213 417 239 207 348 318 396 398
Labelled Cell Count 236 230 348 294 523 464 545 463 401 498 356 292 258 300 345 405 224 442 239 207 348 318 396 398


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.1 23.7 18.5 18.0 17.6 14.9 13.8 14.5 13.2 13.2 11.8 12.5 23.2 22.1 22.9 29.8 27.5 27.7 21.3 21.6 22.7
Std Deviation (1e-4) 2.9 4.4 3.4 2.5 2.7 2.5 2.8 3.0 3.9 2.7 2.6 2.7 4.1 3.6 3.8 8.0 6.7 7.3 4.3 4.8 4.8
Intensity Change (Log2) – – – -0.03 -0.07 -0.3 -0.42 -0.35 -0.48 -0.48 -0.64 -0.56 0.33 0.26 0.31 0.69 0.58 0.59 0.21 0.23 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.4 0.8 1.6 0.3 0 0.9 1.6 2.8 2.4 1.0 -1.1 0.9 1.9 0.8 0.7
Cytoplasm 1.3 0.4 -0.7 -3.1 -4.7 -3.5 -4.5 -3.5 -4.2 4.2 6.7 6.7 2.1 4.3 4.3
Endoplasmic Reticulum -2.7 -2.1 -2.6 -4.5 -2.2 -3.5 -2.3 -3.3 -1.0 -4.8 -6.9 -7.1 -8.6 -6.9 -8.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.6 0 4.8 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.3 2.4 2.9 6.6 6.8 5.3 5.7 3.5 3.3 0 0 0 4.3 1.0 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.1142 20.7924 19.4869 17.4423 16.1794 19.2114 20.5026 22.3587 20.4336 19.4852 20.2267 20.0433 18.5996 21.7244 19.8663 17.4778 18.0363 20.2306
Actin 0.0145 0.0009 0.0025 0.0047 0.0062 0.0012 0.0002 0.0009 0.0004 0.0003 0.0002 0.0002 0.0135 0.0012 0.0046 0.021 0.0041 0.0036
Bud 0.0007 0.0007 0.0005 0.0011 0.0006 0.0001 0 0 0.0002 0 0 0 0.0011 0.0003 0.0001 0.0012 0 0.0001
Bud Neck 0.0015 0.0005 0.001 0.0016 0.0043 0.0029 0.0002 0.0004 0.0004 0.0013 0.0018 0.0017 0.0008 0.0003 0.0007 0.0051 0.0005 0.0016
Bud Periphery 0.0011 0.0001 0.0006 0.0011 0.0011 0.0001 0 0 0.0001 0 0 0 0.0008 0.0001 0.0002 0.0009 0.0001 0.0001
Bud Site 0.0025 0.0006 0.0052 0.0015 0.0004 0.0001 0 0.0001 0.0001 0 0 0 0.0019 0.0014 0.0019 0.0054 0 0.0001
Cell Periphery 0.0005 0.0007 0.0004 0.0003 0.0004 0.0001 0.0002 0.0003 0.0002 0 0 0.0001 0.0003 0.0004 0.0002 0.0003 0.0001 0.0001
Cytoplasm 0.7559 0.9271 0.8664 0.8077 0.8119 0.8687 0.8321 0.8492 0.826 0.9068 0.9019 0.8563 0.7538 0.9005 0.7863 0.7629 0.7927 0.8382
Cytoplasmic Foci 0.0115 0.0018 0.0025 0.0026 0.0021 0.0041 0.0006 0.001 0.0009 0.0009 0.0005 0.0006 0.0099 0.0094 0.0049 0.0114 0.0009 0.0009
Eisosomes 0.0001 0 0 0 0.0001 0 0 0 0 0 0 0 0.0002 0 0.0001 0.0003 0.0001 0
Endoplasmic Reticulum 0.0987 0.0483 0.0815 0.1 0.0481 0.0602 0.1296 0.1265 0.1395 0.0711 0.0686 0.114 0.1094 0.0558 0.1372 0.0718 0.0927 0.12
Endosome 0.0266 0.0009 0.0058 0.0183 0.0216 0.0128 0.0013 0.0008 0.0007 0.0005 0.0003 0.0006 0.0192 0.001 0.0051 0.0197 0.0094 0.0031
Golgi 0.0065 0.0001 0.0037 0.0044 0.0039 0.0033 0 0.0001 0 0 0 0 0.0025 0.0002 0.0019 0.0055 0.0033 0.0004
Lipid Particles 0.003 0.0017 0.0005 0.001 0.0022 0.0025 0.0001 0.0005 0.0003 0.0001 0 0.0001 0.0036 0.0018 0.0027 0.0154 0.0058 0.0002
Mitochondria 0.004 0.0001 0.0034 0.0039 0.014 0.001 0 0 0 0 0 0 0.0035 0.0001 0.0017 0.0153 0.0034 0.0004
None 0.0031 0.0006 0.0012 0.0007 0.0013 0.0007 0.0006 0.0004 0.0005 0.0004 0.0004 0.0005 0.0033 0.0063 0.0134 0.0019 0.0009 0.0004
Nuclear Periphery 0.0266 0.0073 0.0106 0.0234 0.036 0.0163 0.0178 0.0069 0.0147 0.0054 0.0082 0.0086 0.0407 0.0112 0.0221 0.0234 0.0522 0.0137
Nucleolus 0.0004 0 0.0001 0.0001 0.0002 0.0001 0 0 0 0 0 0.0001 0.0006 0.0001 0.0005 0.0009 0.0002 0
Nucleus 0.0273 0.0054 0.0099 0.02 0.0217 0.0198 0.0151 0.0109 0.0144 0.0123 0.0172 0.0155 0.0208 0.0078 0.0121 0.0165 0.0217 0.0151
Peroxisomes 0.0009 0 0.0001 0.0002 0.0001 0.0001 0 0 0 0 0 0 0.0006 0 0.0003 0.009 0.0001 0
Punctate Nuclear 0.0047 0.0005 0.0008 0.0011 0.0008 0.0012 0.0002 0.0003 0.0005 0.0004 0.0004 0.0004 0.0046 0.0006 0.0025 0.0031 0.001 0.0005
Vacuole 0.0086 0.0026 0.0025 0.0049 0.0183 0.0037 0.0016 0.0014 0.001 0.0004 0.0003 0.0011 0.0071 0.0014 0.0013 0.006 0.0078 0.0013
Vacuole Periphery 0.0012 0.0001 0.0004 0.0014 0.0045 0.0009 0.0001 0 0.0001 0 0 0.0001 0.0018 0.0001 0.0004 0.0028 0.0028 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 279.7363 236.1746 187.6224 239.1714 222.3158 190.5296 240.9029 228.1677 229.4605 253.7634
Translational Efficiency 1.3844 1.3806 1.5058 1.2737 1.495 2.0161 1.3302 1.3198 1.3715 1.3852

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2044 968 2161 117 2205 907 254 172 4249 1875 2415 289

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1480.21 1792.12 1836.93 1980.76 1451.27 1562.77 1984.96 1770.41 1465.19 1681.18 1852.50 1855.57
Standard Deviation 228.87 291.45 369.68 322.37 232.36 266.84 353.14 252.99 231.14 302.38 370.77 301.37
Intensity Change Log 2 — 0.275865 0.311495 0.420252 — 0.106790 0.451794 0.286768 — 0.194636 0.382657 0.355711

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000688 0.001302 0.001396 0.004179 0.000578 0.001189 0.001590 0.001590 0.000631 0.001247 0.001416 0.002638
Bud Neck 0.001134 0.003053 0.004128 0.011066 0.001564 0.001631 0.013191 0.007841 0.001357 0.002365 0.005081 0.009147
Bud Site 0.005852 0.014853 0.016985 0.028478 0.005936 0.009112 0.018818 0.019241 0.005896 0.012076 0.017178 0.022981
Cell Periphery 0.000859 0.003773 0.006110 0.018081 0.000896 0.001770 0.008823 0.015957 0.000878 0.002804 0.006396 0.016817
Cytoplasm 0.414825* 0.438085* 0.626921* 0.134185* 0.446454* 0.405949* 0.178115* 0.202750* 0.431239* 0.422540* 0.579718* 0.174992*
Cytoplasmic Foci 0.030910 0.023218 0.012587 0.008899 0.032395 0.025832 0.018154 0.011160 0.031681 0.024482 0.013173 0.010245
Eisosomes 0.000043 0.000065 0.000081 0.000118 0.000039 0.000047 0.000078 0.000067 0.000041 0.000056 0.000081 0.000087
Endoplasmic Reticulum 0.437106* 0.342948* 0.120426* 0.286927* 0.357075* 0.405854* 0.250408* 0.367938* 0.395574* 0.373378* 0.134097* 0.335141*
Endosome 0.008164 0.010626 0.005793 0.025569 0.009271 0.012006 0.028095 0.020796 0.008738 0.011294 0.008138 0.022728
Golgi 0.007128 0.011421 0.006028 0.008674 0.005276 0.009791 0.004469 0.012722 0.006167 0.010633 0.005864 0.011083
Lipid Particles 0.016807 0.047100 0.065888 0.163220* 0.015095 0.032525 0.105932* 0.111163* 0.015919 0.040049 0.070099 0.132238*
Mitochondria 0.000962 0.000483 0.000900 0.000029 0.000979 0.000388 0.000097 0.000056 0.000971 0.000437 0.000815 0.000045
Mitotic Spindle 0.001941 0.006838 0.001051 0.001275 0.000797 0.004683 0.002789 0.005372 0.001348 0.005795 0.001234 0.003713
None 0.004476 0.002823 0.005465 0.000043 0.005294 0.001950 0.000501 0.000352 0.004900 0.002401 0.004942 0.000227
Nuclear Periphery 0.001393 0.000802 0.000393 0.001908 0.005329 0.000592 0.003716 0.001241 0.003435 0.000700 0.000742 0.001511
Nuclear Periphery Foci 0.029321 0.053168 0.031099 0.124865 0.021335 0.053532 0.130517 0.127428 0.025177 0.053344 0.041555 0.126391
Nucleolus 0.000157 0.000093 0.000190 0.000013 0.000241 0.000073 0.000077 0.000026 0.000201 0.000084 0.000178 0.000021
Nucleus 0.012901 0.009548 0.016235 0.004536 0.021373 0.007346 0.012818 0.004171 0.017297 0.008483 0.015876 0.004319
Peroxisomes 0.000228 0.000216 0.000354 0.000275 0.000238 0.000150 0.000451 0.000127 0.000234 0.000184 0.000364 0.000187
Vacuole 0.023534 0.028373 0.076633 0.176772 0.067581 0.023869 0.220598* 0.088218 0.046392 0.026194 0.091775 0.124069
Vacuole Periphery 0.001570 0.001211 0.001338 0.000889 0.002254 0.001712 0.000763 0.001783 0.001925 0.001453 0.001278 0.001421

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.06 -4.91 -2.37 -1.97 -1.87 -2.82 -3.32 -1.96 -0.75 0.66 -4.19 -6.23 -2.95 -2.07 -1.66
Bud Neck -6.30 -12.48 -8.97 -6.99 -6.04 -0.41 -8.89 -5.88 -5.78 3.18 -5.41 -14.28 -9.96 -8.48 -4.82
Bud Site -5.11 -8.82 -2.56 -1.52 -1.24 -2.15 -3.77 -2.18 -1.62 0.33 -5.33 -9.92 -3.36 -2.09 -0.94
Cell Periphery -7.31 -10.38 -5.89 -4.77 -3.94 -5.50 -7.25 -4.82 -4.53 -2.28 -8.72 -11.80 -7.21 -6.26 -4.55
Cytoplasm -1.84 -21.83 14.43 14.52 25.27 3.08 14.89 11.90 9.15 -0.16 0.95 -17.99 17.55 15.76 26.38
Cytoplasmic Foci 3.58 10.94 9.66 5.70 1.42 2.75 3.71 8.26 5.08 2.86 4.48 13.72 12.07 7.25 1.62
Eisosomes -2.42 -5.34 -3.64 -2.58 -2.03 -1.24 -5.23 -1.82 -1.30 0.79 -2.73 -6.68 -3.85 -2.58 -0.86
Endoplasmic Reticulum 7.37 36.08* 6.31 2.78 -5.17 -3.53 7.76 -0.06 1.64 -4.72 2.46 39.17 3.87 2.57 -9.70
Endosome -3.57 5.19 -7.33 -6.08 -8.46 -2.64 -10.09 -6.80 -4.62 2.44 -4.16 1.82 -10.01 -7.64 -10.55
Golgi -4.06 2.05 -0.88 2.39 -2.00 -3.96 2.07 -4.06 -1.29 -4.61 -5.78 0.92 -3.93 -0.06 -4.22
Lipid Particles -8.00 -16.17 -7.92 -6.17 -5.15 -5.41 -8.96 -6.89 -5.50 -0.39 -9.64 -19.02 -10.34 -8.01 -5.26
Mitochondria 3.58 0.52 7.70 7.31 5.42 3.73 7.31 7.88 3.13 2.37 5.18 1.16 10.99 6.43 5.38
Mitotic Spindle -2.79 0.90 0.35 2.97 -0.63 -2.20 -3.16 -1.16 -0.30 -0.65 -3.58 -0.63 -1.09 0.47 -1.00
None 1.83 -0.61 5.66 6.16 5.55 3.33 5.69 6.00 2.55 0.94 3.71 0.16 8.17 5.61 5.36
Nuclear Periphery 3.02 5.51 -1.75 -4.57 -6.62 7.73 2.27 6.40 -3.20 4.89 8.20 8.04 5.38 -5.13 -4.76
Nuclear Periphery Foci -7.07 -1.68 -7.20 -5.21 -6.92 -8.84 -12.35 -9.10 -6.12 -0.27 -11.39 -8.90 -11.55 -8.05 -9.43
Nucleolus 1.67 -0.51 4.01 4.42 14.10 4.79 4.29 7.06 2.31 3.45 4.44 1.15 7.61 4.59 13.38
Nucleus 3.21 -3.88 5.47 3.00 7.78 12.10 1.37 14.35 3.11 6.44 11.38 0.14 14.51 4.65 11.91
Peroxisomes 0.37 -1.90 -0.15 -0.29 0.48 2.66 -1.30 2.46 0.47 2.10 1.66 -2.94 0.74 0.02 2.20
Vacuole -2.34 -21.00 -10.88 -10.49 -7.14 12.03 -11.59 -2.10 -6.74 8.37 9.12 -14.82 -9.19 -11.56 -3.76
Vacuole Periphery 1.38 1.03 2.42 1.59 2.19 1.04 5.44 0.92 -0.04 -2.16 1.65 3.41 1.58 0.22 -0.39
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; functions as a chaperone under ATP-depleted oxidative stress conditions; subunit of GET complex, involved in ATP dependent Golgi to ER trafficking and insertion of tail-anchored (TA) proteins into ER membrane under non-stress conditions; binds as dimer to transmembrane domain (TMD) cargo, shielding TMDs from aqueous solvent; protein abundance increases under DNA replication stress
Localization
Cell Percentages ER (62%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Get3

Get3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Get3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available