Standard name | |
---|---|
Human Ortholog | |
Description | Catalytic subunit of DNA polymerase delta; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 38 | 45 | 17 | 16 | 15 | 17 | 12 | 6 | 1 | 11 | 4 | 6 | 8 | 6 | 21 | 25 | 30 | 30 | 20 | 24 | 7 | 7 | 22 | 14 |
Bud | 2 | 3 | 12 | 9 | 15 | 14 | 15 | 27 | 12 | 14 | 9 | 21 | 2 | 4 | 9 | 2 | 3 | 2 | 7 | 11 | 3 | 4 | 4 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 2 | 0 | 4 | 8 | 2 | 3 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 6 | 3 | 2 | 1 | 0 | 1 | 2 | 1 | 3 | 6 | 10 | 3 | 1 | 4 | 7 | 4 | 6 | 0 | 0 | 0 | 0 | 1 | 0 |
Cytoplasm | 25 | 56 | 30 | 27 | 40 | 33 | 52 | 53 | 27 | 33 | 16 | 32 | 34 | 70 | 83 | 67 | 45 | 52 | 18 | 38 | 14 | 3 | 10 | 2 |
Endoplasmic Reticulum | 14 | 38 | 15 | 6 | 16 | 30 | 13 | 9 | 6 | 23 | 11 | 13 | 14 | 24 | 17 | 13 | 7 | 2 | 11 | 13 | 7 | 4 | 16 | 11 |
Endosome | 17 | 13 | 8 | 6 | 20 | 3 | 15 | 42 | 0 | 1 | 0 | 5 | 4 | 15 | 12 | 26 | 5 | 8 | 42 | 54 | 50 | 11 | 21 | 16 |
Golgi | 101 | 85 | 11 | 4 | 10 | 9 | 6 | 0 | 0 | 2 | 2 | 0 | 40 | 41 | 25 | 106 | 106 | 82 | 84 | 92 | 50 | 27 | 69 | 107 |
Mitochondria | 13 | 38 | 76 | 135 | 128 | 160 | 248 | 276 | 153 | 252 | 154 | 239 | 18 | 5 | 32 | 13 | 10 | 10 | 12 | 12 | 42 | 4 | 5 | 6 |
Nucleus | 0 | 1 | 0 | 2 | 0 | 1 | 2 | 1 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 |
Nucleolus | 2 | 1 | 1 | 2 | 1 | 2 | 3 | 6 | 1 | 2 | 2 | 4 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 |
SpindlePole | 3 | 1 | 2 | 1 | 2 | 1 | 3 | 7 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 5 | 4 | 5 | 4 | 4 | 4 | 0 | 0 | 0 |
Vac/Vac Membrane | 29 | 26 | 13 | 2 | 12 | 10 | 24 | 50 | 3 | 15 | 4 | 13 | 6 | 6 | 4 | 51 | 44 | 30 | 21 | 35 | 18 | 7 | 10 | 5 |
Unique Cell Count | 189 | 232 | 148 | 172 | 207 | 219 | 336 | 373 | 179 | 287 | 176 | 280 | 104 | 151 | 170 | 249 | 196 | 183 | 232 | 297 | 210 | 74 | 167 | 171 |
Labelled Cell Count | 245 | 313 | 189 | 212 | 263 | 280 | 398 | 487 | 209 | 360 | 215 | 354 | 129 | 174 | 208 | 317 | 260 | 230 | 232 | 297 | 210 | 74 | 167 | 171 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.1 | 10.8 | 6.6 | 6.4 | 6.7 | 6.0 | 5.7 | 5.7 | 5.2 | 5.0 | 4.9 | 4.7 | 10.5 | 10.6 | 9.5 | 13.2 | 14.3 | 14.2 | 9.8 | 12.2 | 13.2 |
Std Deviation (1e-4) | 1.8 | 1.6 | 0.9 | 1.1 | 1.2 | 0.9 | 1.1 | 1.1 | 1.2 | 1.0 | 1.2 | 1.1 | 1.9 | 1.4 | 1.4 | 2.5 | 2.6 | 2.2 | 2.1 | 1.8 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.02 | -0.13 | -0.21 | -0.21 | -0.35 | -0.39 | -0.44 | -0.48 | 0.67 | 0.69 | 0.52 | 1.0 | 1.12 | 1.1 | 0.57 | 0.89 | 1.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.6 | -1.4 | -1.2 | -3.4 | -4.9 | -4.3 | -3.1 | 0 | 0 | -1.0 | -2.4 | 0.2 | -0.5 | 1.0 | 1.3 |
Bud | -1.0 | -0.3 | -0.6 | -1.6 | -0.3 | -0.5 | -1.3 | 0 | 0 | -2.1 | -2.1 | -1.0 | -3.8 | -3.0 | -3.2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.1 | -0.2 | -1.3 | -1.3 | -1.7 | -1.2 | -2.5 | -2.9 | -2.5 | 2.2 | 4.8 | 5.3 | 1.5 | 0.6 | 1.7 |
Endoplasmic Reticulum | -2.4 | -0.8 | 1.0 | -2.7 | -3.8 | -2.5 | -0.7 | 0 | 0 | 0.8 | 1.5 | 0 | -1.8 | -2.5 | -3.7 |
Endosome | -0.8 | 1.5 | 0 | -0.4 | 2.0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 0.6 | 1.7 | -1.4 | -0.4 |
Golgi | -2.2 | -1.0 | -1.4 | -3.1 | 0 | 0 | 0 | 0 | 0 | 6.0 | 4.5 | 2.0 | 7.4 | 9.0 | 7.5 |
Mitochondria | 5.1 | 2.0 | 4.3 | 4.8 | 5.0 | 6.7 | 8.4 | 0 | 0 | -5.5 | -9.3 | -6.1 | -10.7 | -9.8 | -9.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -3.2 | -1.1 | -1.6 | -0.6 | 1.5 | -3.0 | -1.4 | -2.6 | -1.7 | -0.9 | -1.7 | -2.5 | 3.1 | 3.4 | 2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 6.5077 | 9.1906 | 8.1769 | 7.8023 | 6.2511 | 8.063 | 8.2315 | 9.4735 | 9.3677 | 8.4408 | 8.2899 | 8.8975 | 9.9879 | 10.8382 | 9.9648 | 9.6683 | 10.1923 | 10.2449 |
Actin | 0.0744 | 0.0099 | 0.0304 | 0.0014 | 0.0261 | 0.0146 | 0.0298 | 0.0121 | 0.0354 | 0.036 | 0.0363 | 0.0056 | 0.0247 | 0.0066 | 0.0205 | 0.024 | 0.031 | 0.0068 |
Bud | 0.0039 | 0.0042 | 0.0069 | 0.0005 | 0.0023 | 0.001 | 0.0008 | 0.0019 | 0.0021 | 0.0006 | 0.0006 | 0.0004 | 0.0008 | 0.0007 | 0.0017 | 0.0014 | 0.0009 | 0.0007 |
Bud Neck | 0.0044 | 0.0021 | 0.003 | 0.0029 | 0.0009 | 0.0093 | 0.001 | 0.0018 | 0.0025 | 0.0049 | 0.0029 | 0.0015 | 0.0017 | 0.0006 | 0.002 | 0.0008 | 0.0041 | 0.0022 |
Bud Periphery | 0.0025 | 0.0033 | 0.0036 | 0.0004 | 0.0023 | 0.0008 | 0.0006 | 0.0007 | 0.0011 | 0.0004 | 0.0007 | 0.0002 | 0.0005 | 0.0002 | 0.0009 | 0.0014 | 0.001 | 0.0004 |
Bud Site | 0.0115 | 0.0233 | 0.0132 | 0.0006 | 0.0053 | 0.0018 | 0.0039 | 0.0181 | 0.0096 | 0.0063 | 0.0034 | 0.0005 | 0.0155 | 0.0068 | 0.0051 | 0.0042 | 0.0035 | 0.001 |
Cell Periphery | 0.0007 | 0.0008 | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0007 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0443 | 0.1118 | 0.049 | 0.0161 | 0.0739 | 0.0423 | 0.0447 | 0.1377 | 0.0679 | 0.0357 | 0.035 | 0.0422 | 0.0591 | 0.1014 | 0.0441 | 0.0092 | 0.0174 | 0.0387 |
Cytoplasmic Foci | 0.0992 | 0.1461 | 0.1221 | 0.0787 | 0.0729 | 0.1059 | 0.1495 | 0.2227 | 0.148 | 0.1625 | 0.169 | 0.1615 | 0.1531 | 0.2094 | 0.1612 | 0.1244 | 0.1848 | 0.1332 |
Eisosomes | 0.0009 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0027 | 0.0002 | 0 | 0.0006 | 0 | 0 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0089 | 0.0087 | 0.0057 | 0.0024 | 0.0019 | 0.0047 | 0.0059 | 0.0026 | 0.0058 | 0.0065 | 0.0031 | 0.0035 | 0.0084 | 0.002 | 0.0027 | 0.0007 | 0.003 | 0.0035 |
Endosome | 0.4216 | 0.4118 | 0.3983 | 0.6045 | 0.4122 | 0.4048 | 0.4509 | 0.3853 | 0.3991 | 0.4355 | 0.4585 | 0.4116 | 0.3852 | 0.4111 | 0.4046 | 0.3885 | 0.2963 | 0.368 |
Golgi | 0.2035 | 0.2125 | 0.2814 | 0.2118 | 0.2606 | 0.3509 | 0.2373 | 0.1849 | 0.2866 | 0.2841 | 0.2001 | 0.3411 | 0.3013 | 0.2392 | 0.3259 | 0.3869 | 0.3965 | 0.4126 |
Lipid Particles | 0.0206 | 0.0081 | 0.0053 | 0.0055 | 0.0502 | 0.0047 | 0.0103 | 0.0022 | 0.004 | 0.0017 | 0.0127 | 0.002 | 0.0059 | 0.0011 | 0.0021 | 0.0218 | 0.0037 | 0.0014 |
Mitochondria | 0.0396 | 0.0181 | 0.0334 | 0.0053 | 0.0475 | 0.0354 | 0.0166 | 0.0066 | 0.0103 | 0.0085 | 0.0533 | 0.0141 | 0.0101 | 0.0076 | 0.0077 | 0.0179 | 0.0059 | 0.0204 |
None | 0.0039 | 0.0004 | 0.0095 | 0.0001 | 0.0011 | 0.0003 | 0.0121 | 0.0005 | 0.001 | 0.0031 | 0.0006 | 0.0002 | 0.0098 | 0.0002 | 0.001 | 0.0001 | 0.0017 | 0.0002 |
Nuclear Periphery | 0.0172 | 0.0015 | 0.0037 | 0.0014 | 0.0029 | 0.0007 | 0.0055 | 0.0004 | 0.0017 | 0.0005 | 0.0014 | 0.0003 | 0.0028 | 0.0003 | 0.001 | 0.0001 | 0.0047 | 0.0004 |
Nucleolus | 0.0005 | 0.0005 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0 | 0.0008 | 0 | 0.0001 | 0.0001 | 0.0059 | 0 |
Nucleus | 0.0053 | 0.0012 | 0.0006 | 0.0004 | 0.0009 | 0.0005 | 0.0014 | 0.0004 | 0.0008 | 0.0003 | 0.0004 | 0.0001 | 0.0023 | 0.0002 | 0.0003 | 0.0001 | 0.0107 | 0.0002 |
Peroxisomes | 0.014 | 0.0043 | 0.0114 | 0.0013 | 0.0152 | 0.0032 | 0.0172 | 0.0075 | 0.0135 | 0.0043 | 0.0141 | 0.0089 | 0.0045 | 0.0065 | 0.0085 | 0.0142 | 0.0064 | 0.0044 |
Punctate Nuclear | 0.005 | 0.0005 | 0.0003 | 0.0003 | 0.004 | 0.0003 | 0.0029 | 0.0005 | 0.0013 | 0.0007 | 0.0021 | 0.0003 | 0.0034 | 0.0002 | 0.0013 | 0.0003 | 0.0194 | 0.0003 |
Vacuole | 0.0113 | 0.024 | 0.0128 | 0.0414 | 0.0121 | 0.0132 | 0.0055 | 0.0106 | 0.0057 | 0.0028 | 0.0024 | 0.003 | 0.0063 | 0.0034 | 0.0057 | 0.0018 | 0.0017 | 0.0028 |
Vacuole Periphery | 0.0069 | 0.0072 | 0.0084 | 0.0245 | 0.0063 | 0.0052 | 0.0034 | 0.0028 | 0.003 | 0.0021 | 0.0029 | 0.0028 | 0.0028 | 0.0025 | 0.0035 | 0.0021 | 0.0013 | 0.0027 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 51.251 | 17.6673 | 18.2206 | 22.4505 | 31.7045 | 31.8049 | 17.2579 | 17.6762 | 17.067 | 33.8013 |
Translational Efficiency | 1.2652 | 1.3145 | 1.0749 | 1.0102 | 1.1444 | 1.7124 | 1.1072 | 0.8224 | 1.0174 | 1.0658 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1472 | 1436 | 2253 | 667 | 1656 | 2101 | 1696 | 355 | 3128 | 3537 | 3949 | 1022 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 872.84 | 1028.60 | 1650.42 | 1257.12 | 1055.49 | 1089.26 | 1298.97 | 1251.17 | 969.54 | 1064.63 | 1499.48 | 1255.05 |
Standard Deviation | 129.48 | 158.44 | 238.53 | 178.75 | 141.17 | 163.99 | 172.73 | 191.49 | 163.56 | 164.48 | 274.85 | 183.30 |
Intensity Change Log 2 | — | 0.236893 | 0.919044 | 0.526333 | — | 0.045435 | 0.299455 | 0.245365 | — | 0.135255 | 0.613065 | 0.379352 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001092 | 0.000942 | 0.003705 | 0.004741 | 0.001642 | 0.000393 | 0.001806 | 0.004476 | 0.001383 | 0.000616 | 0.002889 | 0.004649 |
Bud Neck | 0.000960 | 0.000575 | 0.000260 | 0.003612 | 0.000490 | 0.000495 | 0.001412 | 0.002218 | 0.000711 | 0.000527 | 0.000755 | 0.003128 |
Bud Site | 0.025093 | 0.023702 | 0.009492 | 0.091982 | 0.025372 | 0.014889 | 0.024334 | 0.079735 | 0.025240 | 0.018467 | 0.015866 | 0.087728 |
Cell Periphery | 0.000206 | 0.000131 | 0.000215 | 0.001252 | 0.000162 | 0.000082 | 0.000075 | 0.000212 | 0.000183 | 0.000102 | 0.000155 | 0.000891 |
Cytoplasm | 0.019496 | 0.028887 | 0.018727 | 0.135692* | 0.018887 | 0.017802 | 0.038247 | 0.124605* | 0.019174 | 0.022303 | 0.027110 | 0.131841* |
Cytoplasmic Foci | 0.214591* | 0.254058* | 0.104598 | 0.277223* | 0.244278* | 0.210327* | 0.233203* | 0.278513* | 0.230308* | 0.228082* | 0.159831 | 0.277671* |
Eisosomes | 0.000041 | 0.000024 | 0.000020 | 0.000021 | 0.000037 | 0.000018 | 0.000015 | 0.000025 | 0.000039 | 0.000020 | 0.000018 | 0.000022 |
Endoplasmic Reticulum | 0.001673 | 0.000739 | 0.001305 | 0.000350 | 0.001133 | 0.000443 | 0.001057 | 0.000295 | 0.001387 | 0.000563 | 0.001199 | 0.000331 |
Endosome | 0.114218 | 0.117225 | 0.032516 | 0.132592 | 0.104908 | 0.138993 | 0.185662 | 0.138390 | 0.109289 | 0.130155 | 0.098288 | 0.134606 |
Golgi | 0.500446* | 0.472199* | 0.767467* | 0.176820* | 0.527641* | 0.527050* | 0.276617* | 0.232851* | 0.514843* | 0.504781* | 0.556658* | 0.196283* |
Lipid Particles | 0.002333 | 0.001006 | 0.002670 | 0.003286 | 0.001127 | 0.000568 | 0.002692 | 0.002291 | 0.001694 | 0.000746 | 0.002679 | 0.002940 |
Mitochondria | 0.060850 | 0.022135 | 0.003064 | 0.001112 | 0.028313 | 0.024910 | 0.002921 | 0.001561 | 0.043624 | 0.023784 | 0.003002 | 0.001268 |
Mitotic Spindle | 0.012919 | 0.019428 | 0.015700 | 0.061626 | 0.007979 | 0.010508 | 0.095118 | 0.027274 | 0.010304 | 0.014129 | 0.049808 | 0.049693 |
None | 0.000518 | 0.000134 | 0.000097 | 0.000025 | 0.000074 | 0.000050 | 0.000101 | 0.000028 | 0.000283 | 0.000084 | 0.000099 | 0.000026 |
Nuclear Periphery | 0.000057 | 0.000066 | 0.000135 | 0.000104 | 0.000028 | 0.000074 | 0.000488 | 0.000067 | 0.000042 | 0.000071 | 0.000287 | 0.000091 |
Nuclear Periphery Foci | 0.000757 | 0.000890 | 0.003294 | 0.000982 | 0.000219 | 0.000244 | 0.004117 | 0.000503 | 0.000473 | 0.000507 | 0.003647 | 0.000815 |
Nucleolus | 0.000155 | 0.000076 | 0.000006 | 0.000106 | 0.000090 | 0.000019 | 0.000189 | 0.000082 | 0.000121 | 0.000042 | 0.000085 | 0.000098 |
Nucleus | 0.000298 | 0.000214 | 0.000047 | 0.000272 | 0.000145 | 0.000067 | 0.000351 | 0.000236 | 0.000217 | 0.000127 | 0.000177 | 0.000259 |
Peroxisomes | 0.002858 | 0.001807 | 0.000208 | 0.011164 | 0.001735 | 0.001545 | 0.002918 | 0.014886 | 0.002263 | 0.001651 | 0.001372 | 0.012457 |
Vacuole | 0.013683 | 0.021844 | 0.016899 | 0.084261 | 0.011731 | 0.020520 | 0.095642 | 0.075544 | 0.012649 | 0.021058 | 0.050717 | 0.081233 |
Vacuole Periphery | 0.027757 | 0.033918 | 0.019577 | 0.012777 | 0.024009 | 0.031004 | 0.033036 | 0.016207 | 0.025773 | 0.032187 | 0.025357 | 0.013969 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.47 | -3.31 | -3.00 | -3.06 | -0.60 | 3.57 | -0.36 | -2.11 | -3.19 | -1.99 | 3.37 | -3.01 | -3.51 | -4.44 | -1.62 |
Bud Neck | 3.57 | 7.08 | -7.09 | -8.16 | -8.98 | -0.08 | -8.22 | -5.99 | -5.96 | -3.08 | 2.91 | -0.95 | -9.28 | -9.97 | -9.04 |
Bud Site | 0.54 | 7.70 | -11.99 | -12.27 | -15.32 | 4.65 | 0.24 | -7.93 | -9.68 | -8.16 | 4.08 | 5.77 | -14.38 | -16.28 | -16.93 |
Cell Periphery | 3.91 | -0.40 | -1.20 | -1.29 | -1.19 | 4.16 | 5.09 | -1.85 | -5.31 | -5.99 | 5.98 | 1.62 | -1.25 | -1.40 | -1.30 |
Cytoplasm | -3.24 | 0.11 | -14.83 | -13.55 | -14.99 | 0.53 | -6.81 | -10.12 | -10.23 | -8.08 | -1.82 | -4.88 | -18.03 | -17.53 | -16.59 |
Cytoplasmic Foci | -7.27 | 27.02 | -7.12 | -2.05 | -22.80 | 7.03 | 2.28 | -2.91 | -6.38 | -4.09 | 0.61 | 20.69 | -6.69 | -7.09 | -18.54 |
Eisosomes | 4.63 | 6.82 | 7.70 | 1.02 | 0.06 | 8.16 | 9.13 | 3.29 | -2.14 | -2.85 | 9.24 | 10.36 | 7.87 | -0.82 | -1.79 |
Endoplasmic Reticulum | 2.07 | 0.79 | 2.99 | 4.09 | 7.76 | 3.32 | 0.27 | 4.59 | 1.32 | 3.99 | 3.44 | 0.72 | 4.60 | 3.01 | 8.01 |
Endosome | -0.70 | 25.27 | -2.56 | -2.04 | -20.13 | -8.74 | -17.80 | -4.14 | 0.45 | 6.27 | -7.12 | 3.96 | -4.96 | -0.20 | -7.52 |
Golgi | 3.01 | -33.90* | 31.00* | 28.01 | 62.30* | 0.08 | 28.89 | 20.77 | 21.03 | 3.41 | 1.62 | -5.66 | 38.36 | 37.47 | 40.70* |
Lipid Particles | 3.04 | -0.60 | -2.77 | -5.65 | -2.26 | 1.59 | -3.98 | -2.61 | -3.87 | 0.26 | 3.39 | -2.83 | -4.23 | -7.00 | -1.99 |
Mitochondria | 9.72 | 16.23 | 16.84 | 11.39 | 3.89 | 1.37 | 12.53 | 13.23 | 14.74 | 2.00 | 8.63 | 20.22 | 21.19 | 18.94 | 4.30 |
Mitotic Spindle | -2.74 | -1.63 | -8.13 | -6.87 | -7.60 | -1.78 | -17.90 | -4.29 | -3.78 | 9.78 | -2.98 | -16.45 | -9.32 | -8.37 | -0.41 |
None | 2.06 | 2.26 | 2.64 | 8.24 | 11.38 | 2.74 | -0.49 | 5.18 | 3.90 | 1.31 | 2.27 | 2.02 | 2.91 | 9.27 | 2.96 |
Nuclear Periphery | -0.52 | -3.68 | -3.33 | -2.44 | 0.62 | -1.47 | -6.92 | -2.63 | -0.01 | 6.08 | -1.46 | -7.89 | -4.64 | -1.49 | 5.54 |
Nuclear Periphery Foci | -0.43 | -4.74 | -1.17 | -0.66 | 4.13 | -0.26 | -6.19 | -2.61 | -2.31 | 5.51 | -0.23 | -7.90 | -2.62 | -2.38 | 6.63 |
Nucleolus | 1.20 | 2.48 | 0.59 | -0.64 | -2.22 | 0.98 | -1.04 | -0.01 | -2.59 | 1.35 | 1.61 | 0.59 | 0.30 | -1.78 | -0.36 |
Nucleus | 0.62 | 2.12 | -0.18 | -1.23 | -9.05 | 1.29 | -2.43 | -1.86 | -3.54 | 1.22 | 1.26 | -0.05 | -1.38 | -3.96 | -1.60 |
Peroxisomes | 2.42 | 7.64 | -7.72 | -8.88 | -10.73 | 0.43 | -2.70 | -5.79 | -5.92 | -5.28 | 2.00 | 3.14 | -9.67 | -10.43 | -10.76 |
Vacuole | -5.42 | -2.97 | -14.95 | -13.18 | -14.29 | -7.04 | -25.37 | -10.26 | -8.91 | 2.05 | -8.71 | -22.78 | -18.26 | -16.05 | -8.24 |
Vacuole Periphery | -2.53 | 4.34 | 7.73 | 10.35 | 5.27 | -3.71 | -4.39 | 3.90 | 6.91 | 7.39 | -4.25 | 0.38 | 8.83 | 12.93 | 9.42 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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