Standard name
Human Ortholog
Description UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.94 0.96 0.93 0.96 0.97 0.94 0.82 0.72 0.71 0.79 0.64 0.69 0.99 0.99 1.0 0.96 0.96 0.98 0.91 0.93 0.86 0.97 0.97 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.14 0.15 0.27 0.24 0.41 0.36 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0 0 0 0 0 0.11 0.05 0 0.07 0 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 3 0 0 0 0 1
Bud 1 0 0 0 0 0 0 1 0 1 0 2 1 0 0 0 0 0 1 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 1 1 0 4 1 2 4 1 4 4 8 1 0 1 25 20 27 0 0 0 0 0 0
Cytoplasm 203 142 27 52 148 133 107 131 105 106 76 98 545 556 568 703 523 446 213 136 36 238 383 318
Endoplasmic Reticulum 0 5 1 0 1 1 3 6 6 1 4 5 2 3 0 26 11 14 0 1 0 0 2 5
Endosome 3 0 0 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 1 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 2 0 0 0 0
Mitochondria 2 1 0 2 3 11 18 27 39 32 49 51 0 0 0 5 1 1 0 0 0 0 2 2
Nucleus 0 0 0 1 0 1 1 2 5 0 3 0 0 3 3 26 9 4 1 0 0 0 1 2
Nuclear Periphery 0 0 0 0 3 0 0 0 0 2 2 1 1 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 1 0 1 0 1 0 1 2 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 12 0 0 1 0 1 5 20 7 3 8 6 1 2 0 15 28 5 4 1 0 0 1 3
Unique Cell Count 215 148 29 54 152 141 130 182 147 135 119 143 550 563 570 729 543 453 235 148 42 247 397 346
Labelled Cell Count 226 149 30 56 160 148 137 193 163 150 146 174 552 564 572 803 593 499 235 148 42 247 397 346


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.8 10.2 10.1 8.3 10.2 9.7 9.0 9.0 8.6 8.5 8.5 8.2 11.4 11.3 11.5 15.4 17.5 17.6 8.6 8.6 8.9
Std Deviation (1e-4) 1.5 1.7 1.7 1.2 1.6 2.0 1.7 1.9 1.7 1.6 1.9 1.6 2.1 2.1 2.1 3.0 3.9 3.6 1.5 1.7 1.7
Intensity Change (Log2) – – – -0.28 0.01 -0.05 -0.17 -0.17 -0.23 -0.25 -0.25 -0.29 0.17 0.16 0.19 0.61 0.79 0.8 -0.23 -0.24 -0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 1.2 0.3 -1.4 -2.4 -2.5 -1.8 -3.1 -2.7 2.9 2.4 4.2 0.9 0.9 2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.1 2.9 4.2 3.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9663 5.9243 5.5212 5.6657 4.6423 5.3531 7.2087 8.3035 8.2773 8.4028 7.7939 8.1528 8.0909 9.8513 10.0332 8.844 8.9214 9.2242
Actin 0.0188 0.0023 0.0073 0.0002 0.0326 0.0007 0.0214 0.0008 0.0234 0.0394 0.0152 0.0089 0.0312 0.0004 0.0085 0.0095 0.0169 0.0045
Bud 0.0013 0.0043 0.0022 0.0002 0.0001 0.0005 0.0016 0.0026 0.0005 0.0043 0.0009 0.0004 0.0018 0.0005 0.0012 0.003 0.0381 0.0013
Bud Neck 0.0045 0.0002 0.0005 0.0004 0.0004 0.0012 0.0083 0.0002 0.0033 0.0008 0.0007 0.006 0.0035 0.0003 0.0008 0.002 0.0036 0.0038
Bud Periphery 0.0013 0.0017 0.0007 0 0.0001 0.0003 0.0022 0.0004 0.0004 0.0042 0.0012 0.0003 0.0014 0.0003 0.001 0.0021 0.0169 0.0005
Bud Site 0.0059 0.0074 0.0032 0.0001 0.0002 0.0001 0.014 0.0029 0.0041 0.0028 0.0012 0.0019 0.0055 0.008 0.0045 0.0038 0.034 0.0008
Cell Periphery 0.0005 0.0001 0.0001 0 0 0 0.0004 0.0001 0.0004 0.0002 0.0001 0.0001 0.0003 0.0001 0.0001 0.0003 0.0006 0.0001
Cytoplasm 0.8523 0.9535 0.9262 0.9667 0.8937 0.946 0.8112 0.9596 0.9124 0.7839 0.7662 0.9302 0.7692 0.9684 0.9226 0.828 0.6894 0.9249
Cytoplasmic Foci 0.0092 0.0041 0.0129 0.0028 0.0066 0.0069 0.0267 0.0067 0.0105 0.0345 0.0175 0.0189 0.038 0.0043 0.0082 0.0314 0.0375 0.0159
Eisosomes 0.0005 0 0 0 0.0003 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0005 0 0 0.0001 0.0002 0
Endoplasmic Reticulum 0.0087 0.0023 0.0057 0.0009 0.0016 0.0026 0.0088 0.0008 0.0019 0.0074 0.0017 0.0028 0.0085 0.0018 0.0014 0.0023 0.0158 0.0049
Endosome 0.0159 0.0021 0.0097 0.0046 0.0064 0.0067 0.0203 0.0011 0.0046 0.0369 0.0186 0.0035 0.0267 0.0024 0.0033 0.0405 0.0187 0.0063
Golgi 0.0042 0.0003 0.0021 0.0001 0.0119 0.0009 0.0077 0.0002 0.0023 0.0129 0.0465 0.0019 0.0088 0.0011 0.0009 0.0082 0.0079 0.0019
Lipid Particles 0.0032 0.0001 0.0021 0 0.0153 0.0019 0.0025 0.0001 0.0011 0.0025 0.0026 0.0003 0.0082 0.0001 0.0022 0.0023 0.0043 0.0015
Mitochondria 0.0041 0.0002 0.0012 0.0001 0.0036 0.0007 0.0023 0.0001 0.0007 0.0045 0.0294 0.001 0.0163 0.0004 0.0005 0.0085 0.0396 0.0011
None 0.0465 0.0138 0.0099 0.0115 0.0125 0.0181 0.0383 0.0187 0.0281 0.0323 0.0297 0.0109 0.0413 0.0071 0.0281 0.0305 0.0125 0.0145
Nuclear Periphery 0.0064 0.0012 0.0024 0.0007 0.0016 0.0029 0.0067 0.0006 0.0007 0.0058 0.0072 0.001 0.0057 0.0006 0.0006 0.0018 0.0048 0.0041
Nucleolus 0.0009 0 0.0001 0 0 0.0001 0.0004 0.0001 0 0.0001 0.0002 0.0001 0.0018 0 0.0001 0.0003 0.0006 0.0002
Nucleus 0.0078 0.0044 0.0056 0.0051 0.0065 0.0077 0.0055 0.003 0.0031 0.0058 0.0541 0.0039 0.0064 0.0023 0.0024 0.0085 0.0089 0.0061
Peroxisomes 0.0027 0.0002 0.0039 0 0.0045 0.0003 0.0125 0.0004 0.0012 0.0108 0.0041 0.0047 0.0145 0.0005 0.0124 0.0074 0.0421 0.0034
Punctate Nuclear 0.0026 0.0009 0.0022 0.0002 0.0007 0.001 0.0041 0.001 0.0007 0.0071 0.0009 0.0027 0.0067 0.0003 0.0009 0.0042 0.0031 0.0031
Vacuole 0.0021 0.001 0.0017 0.0057 0.001 0.0012 0.004 0.0005 0.0005 0.0026 0.0013 0.0006 0.0028 0.0008 0.0004 0.0042 0.0031 0.0007
Vacuole Periphery 0.0006 0.0001 0.0003 0.0006 0.0003 0.0003 0.0012 0.0001 0.0001 0.0009 0.0006 0.0001 0.001 0.0001 0.0001 0.0011 0.0013 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 69.6361 41.9588 27.0519 44.0698 46.889 50.7562 34.0787 32.8583 41.1643 56.0034
Translational Efficiency 2.2441 2.0383 2.2442 1.9437 2.5253 3.3168 1.9254 1.8958 1.8924 1.9154

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1261 1017 2235 1558 245 1913 244 1487 1506 2930 2479 3045

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 795.16 962.11 1078.48 1252.36 1082.67 934.87 1395.11 1306.05 841.93 944.32 1109.64 1278.58
Standard Deviation 124.85 161.02 161.66 253.91 661.96 141.69 227.29 260.64 309.19 149.25 193.76 258.61
Intensity Change Log 2 — 0.274957 0.439682 0.655332 — -0.211756 0.365785 0.270617 — 0.014638 0.397540 0.446181

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000113 0.000330 0.000106 0.000660 0.000417 0.000462 0.000547 0.000451 0.000163 0.000417 0.000149 0.000558
Bud Neck 0.001860 0.002394 0.003029 0.004148 0.001044 0.003727 0.001791 0.004213 0.001727 0.003264 0.002907 0.004180
Bud Site 0.003618 0.011350 0.005156 0.018006 0.005985 0.011355 0.008395 0.027179 0.004003 0.011353 0.005475 0.022486
Cell Periphery 0.000087 0.000066 0.000224 0.000137 0.000856 0.000062 0.000147 0.000091 0.000212 0.000063 0.000216 0.000115
Cytoplasm 0.798081* 0.807950* 0.659208* 0.645692* 0.752777* 0.769741* 0.648661* 0.748053* 0.790711* 0.783003* 0.658170* 0.695679*
Cytoplasmic Foci 0.057365 0.061791 0.010682 0.011941 0.089741 0.082738 0.018853 0.018537 0.062632 0.075467 0.011486 0.015162
Eisosomes 0.000016 0.000012 0.000073 0.000015 0.000044 0.000022 0.000013 0.000009 0.000021 0.000018 0.000067 0.000012
Endoplasmic Reticulum 0.000745 0.000720 0.000689 0.001568 0.002911 0.000394 0.000906 0.000452 0.001098 0.000507 0.000710 0.001023
Endosome 0.002188 0.004161 0.001156 0.006595 0.005997 0.006822 0.001375 0.007825 0.002808 0.005898 0.001178 0.007196
Golgi 0.000703 0.001669 0.000080 0.004095 0.000673 0.003323 0.000206 0.009686 0.000698 0.002749 0.000093 0.006825
Lipid Particles 0.000656 0.000763 0.000552 0.001153 0.002736 0.000861 0.001022 0.001520 0.000995 0.000827 0.000598 0.001332
Mitochondria 0.001434 0.000350 0.000321 0.003897 0.000104 0.002372 0.000174 0.001664 0.001218 0.001670 0.000307 0.002807
Mitotic Spindle 0.002003 0.006207 0.009320 0.058823 0.000829 0.012786 0.015412 0.044432 0.001812 0.010502 0.009919 0.051795
None 0.009056 0.006006 0.006697 0.006742 0.002621 0.003718 0.008955 0.006177 0.008009 0.004512 0.006919 0.006466
Nuclear Periphery 0.000137 0.000126 0.000578 0.001097 0.000313 0.000138 0.000583 0.000373 0.000165 0.000134 0.000578 0.000744
Nuclear Periphery Foci 0.001144 0.001849 0.000763 0.001795 0.007065 0.000872 0.004424 0.003901 0.002107 0.001211 0.001123 0.002823
Nucleolus 0.000928 0.000669 0.000359 0.000366 0.000171 0.000585 0.000112 0.001161 0.000805 0.000614 0.000335 0.000754
Nucleus 0.088652 0.044105 0.229265* 0.159644* 0.068707 0.036035 0.181429* 0.049217 0.085407 0.038836 0.224557* 0.105718
Peroxisomes 0.000519 0.001347 0.000160 0.002066 0.000390 0.001834 0.000111 0.001675 0.000498 0.001665 0.000155 0.001875
Vacuole 0.030101 0.046797 0.071409 0.068583 0.056187 0.061277 0.106791 0.069482 0.034345 0.056251 0.074891 0.069022
Vacuole Periphery 0.000593 0.001338 0.000174 0.002975 0.000435 0.000877 0.000094 0.003901 0.000567 0.001037 0.000167 0.003427

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.09 -1.61 -2.76 0.54 -2.22 -0.24 -1.29 3.10 1.69 2.76 -2.62 -1.19 -2.39 1.64 -1.50
Bud Neck -0.96 -2.33 -1.47 -0.31 1.15 -8.06 -5.71 -5.39 2.82 1.41 -3.17 -2.93 -1.98 1.93 1.62
Bud Site -7.17 -1.19 -4.50 1.97 -3.22 -4.13 -2.51 -3.72 -0.23 -0.29 -8.51 -1.55 -6.65 1.08 -4.57
Cell Periphery 3.34 -2.90 0.79 -1.38 3.08 1.28 1.07 1.26 -2.16 6.85 1.46 0.73 1.33 -2.44 4.22
Cytoplasm 0.09 23.73 18.29 16.69 -2.91 -1.35 11.07 3.30 8.01 -10.42 1.84 25.45 17.46 17.15 -9.17
Cytoplasmic Foci -1.19 17.85 17.58 16.71 -0.16 1.04 10.74 11.84 23.03 2.28 -3.97 20.88 20.19 28.73 -2.16
Eisosomes 2.49 -4.23 4.85 2.26 5.22 4.26 3.88 6.81 8.78 7.36 1.63 -3.47 8.98 8.39 5.64
Endoplasmic Reticulum 0.32 -0.80 1.04 0.61 2.16 4.62 3.22 4.91 2.99 4.71 4.40 1.78 4.95 1.01 6.19
Endosome -3.10 5.53 0.68 3.02 -1.93 -0.38 5.92 4.84 6.21 -0.89 -6.07 8.03 2.28 7.11 -3.00
Golgi -1.41 2.98 -0.59 0.81 -2.24 -3.95 3.01 -1.81 1.04 -2.39 -4.00 3.42 -1.79 1.70 -3.30
Lipid Particles -0.61 -0.64 -2.93 -1.72 -2.92 2.27 0.79 1.86 -1.75 4.03 1.21 1.04 -0.41 -2.30 -2.37
Mitochondria 1.47 1.39 0.43 -1.98 -1.85 -3.24 -3.67 -2.34 2.62 -1.72 -0.49 1.35 0.66 1.69 -2.01
Mitotic Spindle -2.08 -5.02 -7.85 -6.10 -4.68 -5.82 -3.69 -8.57 -3.61 -2.10 -5.23 -5.89 -10.94 -6.20 -5.86
None 2.28 2.31 3.06 -0.27 1.37 -1.25 -3.35 -2.66 -1.23 1.79 3.70 1.62 3.27 -1.46 3.08
Nuclear Periphery -0.09 -23.46 -9.38 -9.19 2.39 3.17 -8.32 -3.00 -11.68 7.50 1.23 -24.25 -11.98 -12.81 7.14
Nuclear Periphery Foci -3.33 0.77 -1.70 2.16 -2.57 7.55 2.14 5.88 -3.04 4.65 4.59 3.66 1.94 -2.22 -1.15
Nucleolus 0.62 1.33 1.37 1.00 0.24 -3.19 0.55 -1.53 0.43 -1.60 0.55 1.30 1.06 1.16 -0.70
Nucleus 10.13 -26.45 -7.54 -17.33 17.81 6.36 -7.24 0.05 -14.20 8.10 14.56 -27.90 -3.68 -22.82 26.85
Peroxisomes -1.82 2.31 -0.17 1.26 -1.15 -6.14 5.69 -0.47 5.32 -2.64 -4.72 2.60 -0.08 3.87 -1.80
Vacuole -6.16 -18.65 -22.81 -17.26 -8.39 -0.98 -12.00 -12.70 -16.17 6.36 -9.02 -20.91 -29.37 -23.45 -6.79
Vacuole Periphery -1.01 3.28 -0.51 0.77 -2.81 -3.30 4.49 -1.43 -0.22 -2.45 -1.66 3.70 -1.12 0.51 -3.52
Standard name
Human Ortholog
Description UDP-N-acetylglucosamine pyrophosphorylase; catalyzes the formation of UDP-N-acetylglucosamine (UDP-GlcNAc), which is important in cell wall biosynthesis, protein N-glycosylation, and GPI anchor biosynthesis; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Qri1

Qri1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Qri1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available