Standard name | |
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Human Ortholog | |
Description | Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 0 | 0 | 0 | 3 | 5 | 11 | 5 | 3 | 5 | 4 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 4 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 4 |
Bud Site | 0 | 0 | 0 | 1 | 2 | 10 | 10 | 15 | 6 | 14 | 14 | 5 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 8 | 11 | 9 | 19 | 16 | 14 | 17 | 8 | 13 | 6 | 11 | 60 | 108 | 159 | 27 | 20 | 20 | 4 | 6 | 3 | 18 | 35 | 40 |
Endoplasmic Reticulum | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 9 | 7 | 8 | 0 | 0 | 0 | 0 | 1 | 2 |
Endosome | 2 | 2 | 21 | 14 | 38 | 7 | 2 | 9 | 1 | 0 | 0 | 4 | 2 | 25 | 39 | 13 | 13 | 9 | 12 | 6 | 13 | 7 | 44 | 47 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 1 | 0 | 1 | 1 | 0 | 2 | 0 |
Mitochondria | 112 | 47 | 34 | 62 | 58 | 125 | 228 | 205 | 162 | 275 | 168 | 190 | 5 | 3 | 2 | 34 | 26 | 31 | 7 | 3 | 9 | 8 | 18 | 43 |
Nucleus | 73 | 82 | 69 | 68 | 60 | 56 | 120 | 132 | 126 | 155 | 123 | 112 | 179 | 229 | 223 | 191 | 161 | 148 | 107 | 89 | 64 | 85 | 76 | 54 |
Nuclear Periphery | 3 | 0 | 0 | 1 | 0 | 0 | 1 | 5 | 0 | 5 | 3 | 2 | 0 | 1 | 0 | 11 | 3 | 4 | 10 | 1 | 5 | 0 | 1 | 4 |
Nucleolus | 7 | 1 | 6 | 11 | 20 | 13 | 40 | 21 | 17 | 33 | 16 | 14 | 1 | 2 | 3 | 18 | 18 | 19 | 14 | 10 | 17 | 16 | 17 | 13 |
Peroxisomes | 0 | 0 | 1 | 0 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 1 | 12 | 5 | 24 | 11 | 27 | 23 | 4 | 16 | 2 | 2 | 20 | 19 | 34 | 8 | 12 | 16 | 21 | 6 | 29 | 22 | 47 | 58 |
Vac/Vac Membrane | 27 | 17 | 8 | 19 | 14 | 14 | 5 | 16 | 13 | 9 | 9 | 16 | 12 | 37 | 32 | 31 | 35 | 36 | 52 | 29 | 26 | 42 | 95 | 108 |
Unique Cell Count | 190 | 131 | 132 | 145 | 184 | 176 | 316 | 315 | 242 | 353 | 226 | 250 | 271 | 396 | 457 | 282 | 250 | 242 | 243 | 161 | 179 | 211 | 361 | 408 |
Labelled Cell Count | 233 | 159 | 162 | 190 | 241 | 260 | 460 | 450 | 340 | 525 | 345 | 362 | 285 | 429 | 495 | 345 | 298 | 292 | 243 | 161 | 179 | 211 | 361 | 408 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.6 | 4.3 | 4.3 | 4.0 | 4.5 | 3.6 | 3.5 | 3.6 | 3.5 | 3.4 | 3.2 | 3.4 | 5.4 | 5.6 | 5.4 | 6.6 | 6.7 | 6.3 | 5.5 | 5.8 | 6.1 |
Std Deviation (1e-4) | 0.5 | 0.9 | 0.9 | 0.9 | 1.8 | 1.4 | 1.2 | 1.0 | 0.8 | 0.9 | 1.0 | 1.3 | 1.1 | 1.3 | 1.4 | 1.5 | 1.7 | 1.6 | 1.3 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.07 | -0.27 | -0.31 | -0.27 | -0.29 | -0.34 | -0.44 | -0.34 | 0.34 | 0.39 | 0.33 | 0.62 | 0.64 | 0.56 | 0.36 | 0.44 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 1.6 | 2.3 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.49 | 1.7129 | 1.5144 | 1.0838 | 1.0141 | 1.7475 | 0.6767 | 0.5644 | 0.36 | 1.205 | 0.538 | 0.281 | -0.8269 | -0.7569 | -0.7457 | -0.6946 | -0.8174 | -0.8081 |
Actin | 0.005 | 0.0001 | 0.0002 | 0.014 | 0.0004 | 0.0008 | 0.0306 | 0.0025 | 0.0073 | 0.0614 | 0.028 | 0.011 | 0.0036 | 0.0001 | 0.0037 | 0.0096 | 0.0081 | 0.0047 |
Bud | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0014 | 0.0002 | 0.0004 | 0.0019 | 0.0031 | 0.0003 | 0.0005 | 0 | 0.0001 | 0.0094 | 0.0002 | 0 |
Bud Neck | 0.0014 | 0.0005 | 0.0006 | 0.001 | 0.0025 | 0.0029 | 0.0058 | 0.001 | 0.0014 | 0.0032 | 0.0055 | 0.0039 | 0.001 | 0.0005 | 0.0008 | 0.0018 | 0.0012 | 0.0031 |
Bud Periphery | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0022 | 0.0003 | 0.0004 | 0.0041 | 0.0051 | 0.0004 | 0.0004 | 0 | 0.0003 | 0.0035 | 0.0005 | 0.0001 |
Bud Site | 0.0007 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0003 | 0.0102 | 0.0014 | 0.0037 | 0.0053 | 0.0118 | 0.0005 | 0.0005 | 0.0003 | 0.0004 | 0.0034 | 0.0004 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0.0007 | 0.0001 | 0.0001 | 0.0006 | 0.0008 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0001 | 0 |
Cytoplasm | 0.0268 | 0.0203 | 0.0093 | 0.0033 | 0.0038 | 0.0169 | 0.0051 | 0.0069 | 0.0028 | 0.0038 | 0.004 | 0.0035 | 0.0107 | 0.0011 | 0.0012 | 0.0053 | 0.0078 | 0.0039 |
Cytoplasmic Foci | 0.002 | 0.0003 | 0.0005 | 0.0022 | 0.0002 | 0.0005 | 0.0293 | 0.0007 | 0.0032 | 0.0091 | 0.0054 | 0.0032 | 0.0032 | 0.0001 | 0.0017 | 0.0062 | 0.0157 | 0.0002 |
Eisosomes | 0.0002 | 0 | 0 | 0.0005 | 0 | 0 | 0.0003 | 0 | 0 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0018 | 0.0006 | 0.0006 | 0.0005 | 0.0003 | 0.0005 | 0.0032 | 0.0012 | 0.0002 | 0.0017 | 0.0043 | 0.0045 | 0.0024 | 0.0003 | 0.0003 | 0.0007 | 0.0011 | 0.0002 |
Endosome | 0.0045 | 0.0009 | 0.0022 | 0.0047 | 0.0007 | 0.0008 | 0.0348 | 0.0014 | 0.0024 | 0.0754 | 0.0233 | 0.0085 | 0.0116 | 0.0002 | 0.0021 | 0.0033 | 0.0116 | 0.0007 |
Golgi | 0.0006 | 0 | 0.0002 | 0.0061 | 0 | 0.0005 | 0.0124 | 0.0003 | 0.0015 | 0.0181 | 0.018 | 0.0114 | 0.0012 | 0 | 0.0015 | 0.0014 | 0.0061 | 0.0003 |
Lipid Particles | 0.0018 | 0.0001 | 0.0033 | 0.0138 | 0.0001 | 0.0003 | 0.034 | 0.0002 | 0.0015 | 0.005 | 0.0118 | 0.0058 | 0.0052 | 0.0001 | 0.0034 | 0.0033 | 0.0248 | 0.0009 |
Mitochondria | 0.0014 | 0.0002 | 0.0004 | 0.0129 | 0.0009 | 0.003 | 0.0155 | 0.0008 | 0.0094 | 0.0561 | 0.0737 | 0.0069 | 0.0034 | 0.0002 | 0.0029 | 0.0015 | 0.008 | 0.0006 |
None | 0.011 | 0.0278 | 0.003 | 0.016 | 0.0014 | 0.0044 | 0.003 | 0.0009 | 0.0007 | 0.0019 | 0.0006 | 0.0004 | 0.0162 | 0.0002 | 0.0014 | 0.0071 | 0.018 | 0.0045 |
Nuclear Periphery | 0.0297 | 0.0311 | 0.0411 | 0.0221 | 0.1186 | 0.0071 | 0.0449 | 0.0299 | 0.0153 | 0.0236 | 0.0169 | 0.0086 | 0.0479 | 0.0255 | 0.0408 | 0.0101 | 0.0248 | 0.0092 |
Nucleolus | 0.0631 | 0.0554 | 0.0877 | 0.179 | 0.0705 | 0.1033 | 0.073 | 0.047 | 0.0627 | 0.1344 | 0.1093 | 0.0693 | 0.0645 | 0.0566 | 0.0685 | 0.1685 | 0.1062 | 0.0867 |
Nucleus | 0.7665 | 0.8255 | 0.777 | 0.6583 | 0.6181 | 0.8007 | 0.6143 | 0.8591 | 0.8534 | 0.5239 | 0.5959 | 0.8074 | 0.7833 | 0.8828 | 0.8098 | 0.6946 | 0.6122 | 0.8444 |
Peroxisomes | 0.0024 | 0 | 0.0002 | 0.0075 | 0 | 0.0003 | 0.0256 | 0.0003 | 0.0014 | 0.0036 | 0.0049 | 0.005 | 0.0007 | 0 | 0.0032 | 0.0022 | 0.0036 | 0.0003 |
Punctate Nuclear | 0.0792 | 0.0357 | 0.0722 | 0.0564 | 0.1808 | 0.0571 | 0.0422 | 0.0439 | 0.0309 | 0.0466 | 0.0665 | 0.0451 | 0.0371 | 0.0314 | 0.0566 | 0.0656 | 0.1464 | 0.0395 |
Vacuole | 0.001 | 0.0009 | 0.0005 | 0.0004 | 0.0003 | 0.0003 | 0.0076 | 0.0015 | 0.0008 | 0.0106 | 0.007 | 0.0028 | 0.0037 | 0.0003 | 0.0005 | 0.0014 | 0.0012 | 0.0003 |
Vacuole Periphery | 0.0006 | 0.0004 | 0.0005 | 0.0006 | 0.0011 | 0.0001 | 0.0037 | 0.0006 | 0.0004 | 0.0093 | 0.0039 | 0.001 | 0.0029 | 0.0002 | 0.0007 | 0.0004 | 0.002 | 0.0002 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.4279 | 5.711 | 3.6324 | 9.9213 | 15.9746 | 15.5066 | 5.362 | 5.846 | 6.2485 | 14.2168 |
Translational Efficiency | 0.8932 | 0.8699 | 1.1132 | 0.7738 | 0.5397 | 0.9826 | 0.832 | 0.5978 | 0.9637 | 0.913 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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