Standard name
Human Ortholog
Description 2'-O-ribose methyltransferase; catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.91 0.93 0.92 0.91 0.89 0.81 0.8 0.77 0.75 – 0.7 0.69 0.97 0.98 0.98 0.93 0.85 0.88 0.87 0.88 0.8 0.88 0.9 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.16 0.22 0.15 0.34 0.37 0.4 0.53 – 0.5 0.57 0 0 0 0 0 0 0 0 0.11 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.1 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 0 0 0 2 0 0 0 – 0 0 0 0 0 3 3 5 0 0 0 0 1 2
Bud 0 1 1 2 3 6 8 14 9 – 9 11 1 2 5 1 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 3 1 1 3 2 3 9 5 – 8 10 1 4 8 9 20 29 0 0 0 0 0 0
Cytoplasm 191 229 102 149 255 168 293 430 163 – 154 212 150 315 379 607 362 528 201 218 96 143 229 241
Endoplasmic Reticulum 7 7 3 1 6 5 1 2 0 – 2 1 3 1 1 28 16 27 9 3 4 5 8 21
Endosome 0 1 0 1 3 0 5 4 0 – 0 0 0 1 1 11 5 8 3 2 1 2 0 6
Golgi 1 1 0 0 0 0 0 0 0 – 0 0 0 0 0 1 5 2 2 3 0 0 0 3
Mitochondria 4 6 18 36 43 70 136 222 114 – 110 174 0 1 14 9 11 7 5 5 13 3 4 5
Nucleus 1 0 0 2 1 0 3 4 1 – 1 2 0 0 1 3 1 3 0 1 0 0 0 0
Nuclear Periphery 2 1 0 0 0 0 0 0 0 – 1 0 0 0 1 0 0 1 2 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 – 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 – 0 0 0 0 0 0 1 0 1 0 0 0 0 0
Vac/Vac Membrane 7 1 0 0 4 0 3 8 3 – 3 4 2 2 2 30 41 72 3 8 4 3 8 10
Unique Cell Count 209 245 111 163 285 208 368 555 216 219 307 155 320 386 651 425 603 230 250 121 163 256 298
Labelled Cell Count 216 251 125 193 318 253 454 694 295 288 415 157 326 412 702 465 683 230 250 121 163 256 298


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 7.3 4.8 5.6 5.6 5.0 4.7 4.6 4.1 – 3.9 3.7 8.3 7.8 6.8 9.8 10.9 12.0 7.7 8.0 8.6
Std Deviation (1e-4) 1.2 1.3 0.6 0.9 1.1 1.1 1.2 1.2 1.4 – 1.0 1.0 1.3 1.1 1.1 1.5 1.9 1.7 1.0 1.5 1.5
Intensity Change (Log2) – – – 0.21 0.21 0.05 -0.05 -0.09 -0.24 – -0.33 -0.41 0.79 0.68 0.49 1.02 1.17 1.3 0.67 0.72 0.83


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm -0.1 -0.7 -2.6 -3.0 -3.5 -3.6 – -4.4 -4.8 1.8 3.4 3.3 0.5 -1.8 -1.3
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 1.2 -0.3 3.3 0 0 0 – 0 0 -5.2 0 -4.8 0 -5.7 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 3.4 3.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0347 0.002 0.0056 0.002 0.0797 0.0035 0.0026 0.0009 0.0055 0.002 0.0091 0.0018 0 0.0075 0.0519 0.0037 0.0008 0.0095
Bud 0.001 0.0011 0.0013 0.0006 0.0014 0.0015 0.0021 0.0002 0.001 0.0011 0.0013 0.0002 0.0001 0.0016 0.0021 0.0007 0.0002 0.0006
Bud Neck 0.0097 0.0007 0.0008 0.0014 0.0012 0.0025 0.0006 0.0002 0.0004 0.0006 0.0005 0.0008 0.0001 0.0005 0.0005 0.0006 0.0004 0.0008
Bud Periphery 0.0014 0.0007 0.0012 0.0009 0.0028 0.0038 0.0024 0.0001 0.001 0.002 0.0016 0.0001 0 0.0006 0.0008 0.0005 0 0.0007
Bud Site 0.0094 0.0066 0.0038 0.0009 0.0026 0.0007 0.0022 0.0006 0.0015 0.0009 0.0033 0.0001 0 0.0082 0.0022 0.001 0.0002 0.0004
Cell Periphery 0.0028 0.0016 0.0014 0.0043 0.0018 0.0043 0.0002 0 0.0001 0.0003 0.0002 0 0 0.0003 0.0003 0.0002 0 0.0002
Cytoplasm 0.6535 0.9186 0.7691 0.8176 0.4932 0.7537 0.6971 0.9185 0.8282 0.8228 0.572 0.8423 0.7046 0.8507 0.8366 0.6756 0.8352 0.7127
Cytoplasmic Foci 0.0395 0.0173 0.0379 0.0135 0.0395 0.0339 0.0503 0.0264 0.0325 0.0269 0.0538 0.039 0.075 0.0716 0.0259 0.0508 0.0528 0.0407
Eisosomes 0.0008 0 0.0001 0.0002 0.0004 0.0001 0.0001 0 0 0.0001 0.0002 0 0 0 0.0003 0 0 0.0001
Endoplasmic Reticulum 0.0625 0.0067 0.028 0.0288 0.0087 0.0312 0.0304 0.0045 0.0072 0.0032 0.0047 0.0074 0.0039 0.0079 0.0068 0.005 0.0049 0.0061
Endosome 0.0537 0.0265 0.078 0.0569 0.1589 0.0916 0.125 0.0318 0.0936 0.0667 0.1665 0.0743 0.2015 0.017 0.0373 0.1143 0.0968 0.0854
Golgi 0.0253 0.0056 0.0159 0.0057 0.0231 0.0219 0.0155 0.0052 0.0088 0.0071 0.0373 0.0154 0.0047 0.0113 0.0168 0.0335 0.0025 0.022
Lipid Particles 0.0158 0.0004 0.0014 0.0053 0.0399 0.0014 0.0096 0.0004 0.0005 0.0041 0.0319 0.0014 0.0001 0.0017 0.0009 0.0012 0.0002 0.0003
Mitochondria 0.0068 0.0018 0.0116 0.0069 0.0575 0.0201 0.0055 0.0004 0.0034 0.0369 0.0683 0.0093 0.0004 0.0011 0.0044 0.0359 0.0003 0.0127
None 0.0311 0.0021 0.0281 0.022 0.0228 0.0042 0.0165 0.0015 0.0029 0.0014 0.0019 0.0012 0.0031 0.0014 0.0058 0.0666 0.0014 0.1006
Nuclear Periphery 0.0126 0.0006 0.0024 0.0074 0.0079 0.002 0.0088 0.0005 0.0023 0.0021 0.0046 0.0008 0.0004 0.0013 0.0009 0.0011 0.0004 0.0006
Nucleolus 0.0024 0 0.0001 0.0023 0.005 0.0008 0.0014 0 0 0.0002 0.0007 0 0 0 0 0.0001 0 0
Nucleus 0.0056 0.0008 0.0014 0.0061 0.0025 0.0048 0.0074 0.0006 0.0011 0.0015 0.0018 0.0007 0.0002 0.0008 0.0007 0.001 0.0004 0.0006
Peroxisomes 0.0067 0.0002 0.0004 0.0012 0.0035 0.0036 0.0049 0.0031 0.0005 0.0007 0.0173 0.0007 0.0001 0.0129 0.0004 0.001 0.0002 0.0006
Punctate Nuclear 0.0044 0.0001 0.0006 0.0044 0.0007 0.0038 0.0031 0.0029 0.0003 0.0002 0.0099 0.0003 0.0001 0.0015 0.0003 0.0003 0.0002 0.0005
Vacuole 0.0148 0.0057 0.0081 0.0086 0.0364 0.0068 0.0106 0.0018 0.0067 0.013 0.0086 0.0025 0.004 0.0016 0.0041 0.0047 0.0025 0.0035
Vacuole Periphery 0.0053 0.0008 0.0028 0.0031 0.0105 0.0037 0.0039 0.0005 0.0023 0.0062 0.0047 0.0015 0.0017 0.0006 0.0009 0.0023 0.0007 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 50.0384 40.956 38.5407 38.0106 38.1629 31.2087 34.4703 33.8827 24.8182 45.014
Translational Efficiency 0.6298 0.7389 0.6685 0.5751 0.6499 0.6111 0.6477 0.4316 0.5484 0.4669

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1213 653 2343 1106 911 1777 241 2481 2124 2430 2584 3587

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 651.86 1068.32 1103.57 1097.35 870.48 945.89 1173.36 1101.83 745.63 978.79 1110.08 1100.45
Standard Deviation 93.02 143.98 180.89 137.44 110.39 128.71 143.85 149.75 147.90 143.64 178.92 146.08
Intensity Change Log 2 — 0.712710 0.759544 0.751390 — 0.119861 0.430763 0.340019 — 0.403923 0.580799 0.530675

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000218 0.001204 0.000330 0.001363 0.000293 0.000454 0.000536 0.000959 0.000250 0.000655 0.000349 0.001083
Bud Neck 0.016775 0.002700 0.002133 0.002625 0.003686 0.004462 0.003559 0.003484 0.011161 0.003989 0.002266 0.003219
Bud Site 0.024937 0.044105 0.013489 0.028770 0.020894 0.027051 0.022599 0.034418 0.023203 0.031634 0.014339 0.032676
Cell Periphery 0.000462 0.000188 0.000065 0.000077 0.000156 0.000114 0.000096 0.000065 0.000331 0.000134 0.000068 0.000069
Cytoplasm 0.449542* 0.535983* 0.774460* 0.552609* 0.586889* 0.453045* 0.588630* 0.690588* 0.508451* 0.475333* 0.757129* 0.648044*
Cytoplasmic Foci 0.323719* 0.279096* 0.054184 0.112370 0.269655* 0.236747* 0.135093 0.125939 0.300531* 0.248127* 0.061730 0.121755
Eisosomes 0.000291 0.000031 0.000005 0.000015 0.000050 0.000033 0.000022 0.000013 0.000187 0.000033 0.000007 0.000014
Endoplasmic Reticulum 0.003383 0.002273 0.001019 0.001546 0.003590 0.002655 0.000890 0.000368 0.003472 0.002552 0.001007 0.000731
Endosome 0.028377 0.029120 0.004105 0.020673 0.022197 0.052145 0.015351 0.020985 0.025726 0.045958 0.005154 0.020889
Golgi 0.021580 0.022227 0.002073 0.010612 0.018542 0.022018 0.000970 0.013275 0.020277 0.022074 0.001970 0.012454
Lipid Particles 0.008807 0.002010 0.001387 0.001618 0.002811 0.002676 0.003341 0.002546 0.006235 0.002497 0.001569 0.002260
Mitochondria 0.027937 0.002968 0.000540 0.004039 0.004245 0.004646 0.000464 0.001593 0.017776 0.004195 0.000533 0.002347
Mitotic Spindle 0.000256 0.003948 0.029918 0.039500 0.002071 0.010670 0.017585 0.012586 0.001034 0.008864 0.028768 0.020885
None 0.003784 0.000216 0.002276 0.000465 0.000923 0.000408 0.000634 0.000182 0.002557 0.000356 0.002123 0.000269
Nuclear Periphery 0.000150 0.000040 0.000518 0.000318 0.000045 0.000117 0.000422 0.000088 0.000105 0.000096 0.000509 0.000159
Nuclear Periphery Foci 0.000434 0.000387 0.003541 0.001536 0.000502 0.000367 0.008817 0.000676 0.000463 0.000373 0.004033 0.000941
Nucleolus 0.000774 0.000079 0.000343 0.000281 0.000168 0.000521 0.000162 0.000177 0.000514 0.000402 0.000326 0.000209
Nucleus 0.021024 0.002427 0.069847 0.009864 0.006182 0.005439 0.019342 0.004899 0.014658 0.004629 0.065137 0.006430
Peroxisomes 0.003230 0.002293 0.000584 0.003514 0.002129 0.002472 0.001833 0.005998 0.002758 0.002424 0.000701 0.005232
Vacuole 0.049906 0.055842 0.037731 0.203393* 0.048237 0.154696 0.179258 0.077047 0.049190 0.128131 0.050931 0.116004
Vacuole Periphery 0.014414 0.012865 0.001451 0.004812 0.006732 0.019262 0.000396 0.004114 0.011119 0.017543 0.001352 0.004329

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.54 -3.69 -2.70 0.57 -1.95 -0.90 -2.72 -4.19 -1.89 -2.49 -1.89 -3.51 -5.24 -0.79 -3.91
Bud Neck 13.01 13.24 13.47 0.98 0.87 -1.47 0.71 0.73 3.10 -0.27 10.45 12.78 12.25 3.42 -1.93
Bud Site -4.98 4.95 1.99 6.72 -3.18 -3.20 1.01 -5.43 -1.80 -4.39 -4.41 5.61 -2.40 2.52 -8.75
Cell Periphery 6.34 10.34 10.65 5.45 1.16 2.11 4.77 10.34 2.95 4.99 7.55 11.31 12.29 5.01 1.84
Cytoplasm -6.28 -34.74 -8.65 -0.83 19.55 11.43 2.45 -9.57 -25.20 -8.12 3.89 -28.91 -18.08 -21.24 11.66
Cytoplasmic Foci 4.44 44.38 35.54 20.63 -7.13 3.90 15.03 19.95 20.10 -0.83 8.56 48.97 37.93 28.03 -14.13
Eisosomes 14.01 15.48 15.13 5.42 -6.55 3.38 4.94 8.12 10.30 6.31 14.00 16.31 15.95 11.63 -5.75
Endoplasmic Reticulum 1.81 4.90 4.18 2.37 0.62 1.21 4.16 4.99 5.28 4.47 1.83 6.05 7.44 5.65 3.42
Endosome -0.23 16.63 8.68 6.43 -7.10 -12.81 5.49 2.44 16.30 -4.13 -11.06 19.83 7.52 17.54 -14.97
Golgi -0.25 16.94 9.46 8.16 -2.47 -1.94 15.37 5.61 7.41 -12.73 -1.27 22.01 10.61 10.44 -10.38
Lipid Particles 11.38 13.06 13.16 1.55 1.21 0.33 -0.38 0.97 0.41 1.01 9.27 12.94 11.51 1.02 -2.40
Mitochondria 9.76 12.37 11.10 0.68 -1.87 -0.26 3.01 2.18 4.00 -3.13 8.72 12.26 11.28 3.66 -3.20
Mitotic Spindle -2.62 -13.27 -6.96 -5.47 1.49 -4.68 -3.18 -6.13 -0.55 0.55 -5.89 -13.17 -9.87 -3.53 5.19
None 4.46 2.01 4.13 -3.68 13.76 1.87 0.30 2.71 2.54 4.03 4.64 1.07 4.81 1.07 17.24
Nuclear Periphery 5.06 -11.03 -4.28 -6.91 4.98 -4.69 -7.28 -3.04 0.50 6.34 0.51 -13.51 -3.58 -4.11 10.92
Nuclear Periphery Foci 0.17 -7.51 -3.72 -7.32 5.90 1.25 -4.85 -1.36 -2.01 4.67 0.54 -9.13 -2.71 -4.44 8.15
Nucleolus 2.45 1.26 1.83 -6.58 3.97 -1.59 -0.70 -0.89 1.47 0.13 0.45 0.72 1.83 1.17 5.90
Nucleus 10.23 -21.20 2.40 -15.19 27.88 1.08 -7.15 -1.59 -3.64 6.91 9.08 -27.22 4.16 -11.89 32.90
Peroxisomes 2.62 14.21 3.45 0.31 -6.03 -0.38 1.08 -3.44 -6.95 -8.85 0.86 5.65 -3.40 -6.20 -13.43
Vacuole -1.72 -1.62 -27.93 -24.76 -27.75 -19.29 -13.29 -14.02 10.16 10.13 -18.46 -7.75 -27.65 -4.56 -18.46
Vacuole Periphery 0.80 12.28 9.98 5.56 -1.93 -9.14 9.09 4.21 12.93 -8.23 -5.33 14.17 10.76 14.02 -5.29
Standard name
Human Ortholog
Description 2'-O-ribose methyltransferase; catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs
Localization
Cell Percentages cytoplasm (86%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Trm3

Trm3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trm3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available