Standard name | |
---|---|
Human Ortholog | |
Description | Sorting nexin family member; required for the cytoplasm-to-vacuole targeting (Cvt) pathway and for endosomal sorting; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 7 | 8 | 5 | 6 | 5 | 1 | 8 | 15 | 3 | 7 | 7 | 5 | 6 | 5 | 0 | 20 | 9 | 18 | 6 | 5 | 3 | 0 | 1 | 2 |
Bud | 7 | 2 | 2 | 1 | 2 | 7 | 9 | 11 | 13 | 19 | 8 | 10 | 2 | 0 | 1 | 2 | 0 | 3 | 1 | 1 | 1 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 1 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 58 | 50 | 39 | 25 | 30 | 26 | 48 | 43 | 30 | 40 | 31 | 30 | 23 | 49 | 79 | 36 | 36 | 46 | 27 | 13 | 12 | 11 | 13 | 19 |
Endoplasmic Reticulum | 6 | 9 | 2 | 1 | 2 | 5 | 4 | 3 | 2 | 1 | 1 | 0 | 3 | 3 | 1 | 6 | 8 | 5 | 5 | 2 | 2 | 0 | 2 | 2 |
Endosome | 157 | 109 | 48 | 49 | 66 | 51 | 68 | 44 | 30 | 42 | 28 | 42 | 60 | 69 | 71 | 95 | 75 | 135 | 223 | 157 | 86 | 78 | 100 | 95 |
Golgi | 0 | 5 | 0 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 13 | 10 | 7 | 2 | 6 | 1 | 0 | 1 | 0 |
Mitochondria | 35 | 2 | 1 | 10 | 3 | 21 | 25 | 27 | 58 | 59 | 73 | 61 | 0 | 0 | 0 | 17 | 8 | 11 | 20 | 5 | 24 | 10 | 13 | 9 |
Nucleus | 0 | 1 | 1 | 0 | 1 | 1 | 2 | 4 | 1 | 0 | 4 | 2 | 1 | 0 | 0 | 2 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 2 | 3 | 0 | 12 | 6 | 5 | 9 | 13 | 6 | 13 | 12 | 10 | 0 | 1 | 4 | 11 | 7 | 10 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 7 | 2 | 1 | 5 | 7 | 5 | 9 | 2 | 3 | 3 | 4 | 5 | 2 | 3 | 9 | 1 | 1 | 6 | 0 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 45 | 24 | 19 | 67 | 70 | 31 | 72 | 62 | 18 | 49 | 18 | 50 | 23 | 30 | 30 | 61 | 57 | 76 | 13 | 8 | 6 | 10 | 8 | 13 |
Vac/Vac Membrane | 19 | 18 | 11 | 8 | 11 | 3 | 9 | 4 | 6 | 8 | 2 | 6 | 11 | 7 | 8 | 35 | 31 | 28 | 12 | 14 | 14 | 9 | 21 | 9 |
Unique Cell Count | 272 | 183 | 111 | 146 | 164 | 126 | 201 | 176 | 132 | 182 | 147 | 171 | 105 | 135 | 163 | 212 | 180 | 257 | 319 | 224 | 162 | 127 | 171 | 158 |
Labelled Cell Count | 343 | 233 | 129 | 187 | 205 | 156 | 269 | 229 | 172 | 243 | 191 | 225 | 132 | 167 | 205 | 301 | 246 | 347 | 319 | 224 | 162 | 127 | 171 | 158 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.5 | 7.4 | 7.1 | 6.6 | 7.7 | 6.6 | 6.6 | 7.0 | 5.6 | 6.1 | 5.7 | 6.1 | 7.4 | 7.5 | 7.6 | 10.5 | 11.8 | 9.8 | 6.6 | 6.9 | 7.3 |
Std Deviation (1e-4) | 0.7 | 0.9 | 1.6 | 1.6 | 1.8 | 1.7 | 1.4 | 1.5 | 1.2 | 1.2 | 1.2 | 1.6 | 1.2 | 1.1 | 1.3 | 2.1 | 3.4 | 2.4 | 1.5 | 1.2 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.11 | -0.11 | -0.11 | -0.03 | -0.36 | -0.22 | -0.32 | -0.22 | 0.06 | 0.07 | 0.1 | 0.56 | 0.72 | 0.46 | -0.11 | -0.04 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0.1 | 0 | 0.4 | 0 | 0 | 1.6 | 0.2 | 0.9 |
Bud | 0 | 0 | 0 | 0 | 1.8 | 2.6 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -3.3 | -3.2 | -2.5 | -2.1 | -2.0 | -2.1 | -2.5 | -2.5 | -3.4 | -2.1 | 0.2 | 2.2 | -3.7 | -2.9 | -3.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -1.6 | -0.5 | -0.4 | -1.6 | -3.2 | -3.4 | -3.6 | -4.2 | -3.3 | 2.0 | 1.2 | 0.1 | 0.3 | -0.3 | 1.6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.2 | 3.5 | 4.0 | 7.8 | 6.5 | 8.6 | 6.9 | 0 | 0 | 0 | 2.6 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 3.1 | 0 | 0 | 0 | 2.9 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 4.8 | 4.4 | 1.4 | 3.5 | 3.3 | -0.8 | 1.9 | -1.1 | 2.3 | 0.9 | 1.0 | 0.3 | 2.3 | 2.7 | 2.5 |
Vacuole | -1.3 | -1.0 | -2.5 | -1.9 | -2.8 | -1.6 | -1.9 | -3.1 | -2.2 | 0.1 | -1.4 | -1.6 | 1.6 | 1.7 | 0.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2617 | 0.9006 | 0.8532 | 0.5274 | 0.3132 | 0.864 | 1.2562 | 1.509 | 1.395 | 1.2742 | 1.0872 | 1.5482 | 1.3483 | 1.6877 | 1.2594 | 1.3175 | 2.0967 | 1.387 |
Actin | 0.021 | 0.0034 | 0.004 | 0.0001 | 0.0008 | 0.0021 | 0.0032 | 0.001 | 0.0016 | 0.0036 | 0.0146 | 0.0038 | 0.0284 | 0.0002 | 0.022 | 0 | 0.0683 | 0.029 |
Bud | 0.0006 | 0.0029 | 0.0043 | 0.0002 | 0.0001 | 0.0007 | 0.0003 | 0.0009 | 0.0021 | 0.0005 | 0.0006 | 0.0003 | 0.0035 | 0.0006 | 0.0017 | 0.0002 | 0.0026 | 0.0003 |
Bud Neck | 0.0011 | 0.001 | 0.0006 | 0.0002 | 0.0004 | 0.001 | 0.0007 | 0.0005 | 0.0011 | 0.0019 | 0.0005 | 0.0022 | 0.0021 | 0.0004 | 0.0011 | 0.0003 | 0.0106 | 0.001 |
Bud Periphery | 0.0006 | 0.0013 | 0.0021 | 0.0001 | 0.0001 | 0.0007 | 0.0003 | 0.0009 | 0.0032 | 0.0008 | 0.0007 | 0.0004 | 0.0022 | 0.0003 | 0.0017 | 0.0001 | 0.0035 | 0.0004 |
Bud Site | 0.0049 | 0.0242 | 0.0028 | 0.0004 | 0.0004 | 0.0005 | 0.0028 | 0.0053 | 0.0047 | 0.0006 | 0.0009 | 0.0007 | 0.0142 | 0.0025 | 0.0087 | 0.0002 | 0.0408 | 0.0007 |
Cell Periphery | 0.0003 | 0.0005 | 0.0002 | 0 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0011 | 0.0001 | 0.0002 | 0 | 0.0011 | 0.0001 |
Cytoplasm | 0.1258 | 0.2878 | 0.1846 | 0.1636 | 0.1081 | 0.2274 | 0.1196 | 0.1029 | 0.1626 | 0.1503 | 0.051 | 0.1411 | 0.1529 | 0.3051 | 0.1835 | 0.1538 | 0.0524 | 0.2009 |
Cytoplasmic Foci | 0.258 | 0.2429 | 0.2618 | 0.2235 | 0.224 | 0.3097 | 0.2317 | 0.3364 | 0.2671 | 0.228 | 0.2254 | 0.2968 | 0.2109 | 0.2619 | 0.2563 | 0.1782 | 0.1998 | 0.3382 |
Eisosomes | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0004 | 0.0002 |
Endoplasmic Reticulum | 0.0071 | 0.0046 | 0.0034 | 0.0036 | 0.002 | 0.0032 | 0.0045 | 0.0025 | 0.0029 | 0.0041 | 0.0022 | 0.0029 | 0.0132 | 0.0043 | 0.0037 | 0.0028 | 0.0041 | 0.0042 |
Endosome | 0.2982 | 0.2854 | 0.3413 | 0.3186 | 0.4566 | 0.3079 | 0.4303 | 0.4289 | 0.3683 | 0.4372 | 0.5393 | 0.4189 | 0.3632 | 0.3269 | 0.3785 | 0.5635 | 0.409 | 0.2981 |
Golgi | 0.0252 | 0.011 | 0.0168 | 0.0074 | 0.0321 | 0.0173 | 0.0361 | 0.0294 | 0.0265 | 0.0282 | 0.0573 | 0.0348 | 0.0265 | 0.015 | 0.0367 | 0.0164 | 0.0765 | 0.0256 |
Lipid Particles | 0.0493 | 0.0137 | 0.0295 | 0.0522 | 0.0367 | 0.0327 | 0.0465 | 0.0243 | 0.0408 | 0.0056 | 0.0133 | 0.0216 | 0.0558 | 0.0176 | 0.0183 | 0.0059 | 0.0422 | 0.0337 |
Mitochondria | 0.0138 | 0.0058 | 0.0044 | 0.0017 | 0.0311 | 0.0026 | 0.0084 | 0.0024 | 0.0208 | 0.0467 | 0.0138 | 0.0151 | 0.0064 | 0.0018 | 0.0052 | 0.0007 | 0.0176 | 0.0112 |
None | 0.0764 | 0.0801 | 0.0858 | 0.1792 | 0.0776 | 0.0586 | 0.0653 | 0.0238 | 0.0532 | 0.0201 | 0.0127 | 0.0212 | 0.0412 | 0.0248 | 0.0355 | 0.0318 | 0.0088 | 0.025 |
Nuclear Periphery | 0.021 | 0.0014 | 0.0027 | 0.0026 | 0.0012 | 0.0007 | 0.0092 | 0.0008 | 0.0008 | 0.0025 | 0.001 | 0.0007 | 0.0107 | 0.001 | 0.0011 | 0.0013 | 0.0088 | 0.0026 |
Nucleolus | 0.0019 | 0.0021 | 0.0038 | 0.0005 | 0.0002 | 0.0002 | 0.0005 | 0.0003 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0077 | 0.0002 | 0.0002 | 0.0001 | 0.0075 | 0.0004 |
Nucleus | 0.0081 | 0.0024 | 0.0084 | 0.0013 | 0.0006 | 0.0008 | 0.0024 | 0.0005 | 0.0008 | 0.0015 | 0.0005 | 0.0004 | 0.0096 | 0.0008 | 0.0005 | 0.0007 | 0.0029 | 0.0006 |
Peroxisomes | 0.0307 | 0.0062 | 0.0176 | 0.0156 | 0.0143 | 0.0204 | 0.0192 | 0.0244 | 0.0237 | 0.0418 | 0.0523 | 0.0279 | 0.0196 | 0.0126 | 0.0292 | 0.0016 | 0.0208 | 0.0153 |
Punctate Nuclear | 0.0181 | 0.0025 | 0.0076 | 0.0028 | 0.0028 | 0.0046 | 0.0024 | 0.0016 | 0.0014 | 0.0008 | 0.0007 | 0.0012 | 0.0083 | 0.0023 | 0.0019 | 0.0007 | 0.0061 | 0.0056 |
Vacuole | 0.0272 | 0.0194 | 0.0157 | 0.0223 | 0.0077 | 0.0071 | 0.0138 | 0.011 | 0.0146 | 0.0201 | 0.0095 | 0.0077 | 0.0189 | 0.0191 | 0.0112 | 0.036 | 0.0133 | 0.0052 |
Vacuole Periphery | 0.0104 | 0.0014 | 0.0027 | 0.0039 | 0.0031 | 0.0015 | 0.0023 | 0.0019 | 0.0028 | 0.0053 | 0.0033 | 0.002 | 0.0033 | 0.0024 | 0.0028 | 0.0057 | 0.003 | 0.0018 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.3283 | 11.2846 | 11.038 | 13.1969 | 11.9032 | 10.3226 | 11.7988 | 13.6516 | 14.7088 | 12.5684 |
Translational Efficiency | 0.8102 | 0.871 | 0.8504 | 0.8352 | 0.7337 | 1.0108 | 0.9151 | 0.8435 | 0.8463 | 0.7834 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1923 | 1139 | 101 | 939 | 758 | 2232 | 210 | 76 | 2681 | 3371 | 311 | 1015 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 704.49 | 664.89 | 1171.53 | 981.71 | 823.75 | 809.07 | 1040.26 | 1109.47 | 738.21 | 760.35 | 1082.89 | 991.28 |
Standard Deviation | 85.63 | 107.71 | 145.38 | 139.00 | 94.94 | 109.33 | 131.08 | 158.91 | 103.40 | 128.39 | 149.15 | 144.55 |
Intensity Change Log 2 | — | -0.083464 | 0.733743 | 0.478718 | — | -0.025942 | 0.336666 | 0.429592 | — | -0.052174 | 0.533343 | 0.452446 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002802 | 0.000325 | 0.016022 | 0.000765 | 0.000420 | 0.000455 | 0.000361 | 0.003118 | 0.002129 | 0.000411 | 0.005447 | 0.000941 |
Bud Neck | 0.033078 | 0.018362 | 0.012146 | 0.012927 | 0.015759 | 0.011420 | 0.017099 | 0.005521 | 0.028182 | 0.013766 | 0.015490 | 0.012372 |
Bud Site | 0.023045 | 0.016634 | 0.029708 | 0.044842 | 0.010302 | 0.015534 | 0.013112 | 0.060195 | 0.019442 | 0.015906 | 0.018502 | 0.045992 |
Cell Periphery | 0.000243 | 0.000114 | 0.000118 | 0.000053 | 0.000131 | 0.000058 | 0.000106 | 0.000072 | 0.000211 | 0.000077 | 0.000110 | 0.000054 |
Cytoplasm | 0.089682 | 0.115619* | 0.004062 | 0.074949 | 0.061677 | 0.041009 | 0.028325 | 0.138041* | 0.081764 | 0.066218 | 0.020445 | 0.079673 |
Cytoplasmic Foci | 0.344452* | 0.302093* | 0.276472* | 0.183779* | 0.240125* | 0.257787* | 0.121748 | 0.279779* | 0.314956* | 0.272757* | 0.171996 | 0.190967* |
Eisosomes | 0.000875 | 0.000097 | 0.000052 | 0.000024 | 0.000123 | 0.000060 | 0.000099 | 0.000024 | 0.000662 | 0.000073 | 0.000083 | 0.000024 |
Endoplasmic Reticulum | 0.001194 | 0.001147 | 0.001436 | 0.000648 | 0.001525 | 0.000452 | 0.001169 | 0.000123 | 0.001288 | 0.000687 | 0.001256 | 0.000608 |
Endosome | 0.209244* | 0.311475* | 0.327472* | 0.312746* | 0.491294* | 0.468736* | 0.439127* | 0.243067* | 0.288988* | 0.415601* | 0.402866* | 0.307529* |
Golgi | 0.157862* | 0.042791 | 0.049672 | 0.012422 | 0.043087 | 0.064210 | 0.004640 | 0.004356 | 0.125412* | 0.056973 | 0.019265 | 0.011818 |
Lipid Particles | 0.031666 | 0.021048 | 0.017047 | 0.005126 | 0.026479 | 0.012514 | 0.014288 | 0.007488 | 0.030199 | 0.015397 | 0.015184 | 0.005303 |
Mitochondria | 0.032099 | 0.017290 | 0.000701 | 0.002107 | 0.006066 | 0.016483 | 0.000590 | 0.000027 | 0.024739 | 0.016755 | 0.000626 | 0.001952 |
Mitotic Spindle | 0.001824 | 0.006807 | 0.081087 | 0.090965 | 0.003343 | 0.020984 | 0.043072 | 0.049158 | 0.002254 | 0.016194 | 0.055418 | 0.087835 |
None | 0.000690 | 0.003247 | 0.000019 | 0.000100 | 0.000297 | 0.000405 | 0.000293 | 0.000030 | 0.000579 | 0.001365 | 0.000204 | 0.000095 |
Nuclear Periphery | 0.000049 | 0.000449 | 0.000707 | 0.000514 | 0.000298 | 0.000149 | 0.000975 | 0.000101 | 0.000119 | 0.000250 | 0.000888 | 0.000483 |
Nuclear Periphery Foci | 0.000800 | 0.003619 | 0.020016 | 0.005292 | 0.001732 | 0.001208 | 0.031659 | 0.001989 | 0.001064 | 0.002023 | 0.027878 | 0.005044 |
Nucleolus | 0.000676 | 0.003621 | 0.001399 | 0.000798 | 0.000392 | 0.000669 | 0.010565 | 0.000118 | 0.000596 | 0.001666 | 0.007589 | 0.000747 |
Nucleus | 0.003523 | 0.013632 | 0.001234 | 0.002401 | 0.002737 | 0.001238 | 0.031623 | 0.001038 | 0.003301 | 0.005426 | 0.021754 | 0.002299 |
Peroxisomes | 0.045058 | 0.019313 | 0.061785 | 0.029230 | 0.022675 | 0.021325 | 0.044051 | 0.071816 | 0.038729 | 0.020645 | 0.049810 | 0.032419 |
Vacuole | 0.016582 | 0.090441 | 0.092368 | 0.213869* | 0.064743 | 0.056674 | 0.193963 | 0.133424 | 0.030199 | 0.068083 | 0.160969 | 0.207846* |
Vacuole Periphery | 0.004558 | 0.011876 | 0.006476 | 0.006443 | 0.006794 | 0.008629 | 0.003134 | 0.000516 | 0.005190 | 0.009726 | 0.004219 | 0.005999 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 5.20 | -1.39 | 4.15 | -2.05 | 1.62 | -0.34 | 0.18 | -1.43 | -1.41 | -1.44 | 4.97 | -1.06 | 3.21 | -2.09 | 1.49 |
Bud Neck | 5.56 | 5.86 | 8.17 | 2.73 | -0.26 | 2.17 | -0.24 | 5.37 | 5.61 | 4.02 | 7.98 | 5.00 | 8.04 | 1.06 | 1.18 |
Bud Site | 3.25 | -1.49 | -5.82 | -7.92 | -2.03 | -3.16 | -1.80 | -3.72 | -3.29 | -3.38 | 2.35 | -0.10 | -7.61 | -8.85 | -6.59 |
Cell Periphery | 4.43 | 3.74 | 6.89 | 6.04 | 4.22 | 4.22 | 0.90 | 2.68 | -0.64 | 1.83 | 6.46 | 4.14 | 7.65 | 3.63 | 4.84 |
Cytoplasm | -4.23 | 22.33 | -1.09 | 2.62 | -16.03 | 3.61 | 3.56 | -3.92 | -4.66 | -4.82 | 3.58 | 11.39 | -3.27 | -5.63 | -11.18 |
Cytoplasmic Foci | 5.42 | 3.64 | 22.96 | 15.15 | 4.39 | -2.04 | 10.46 | -0.41 | 0.34 | -5.63 | 8.01 | 13.72 | 19.77 | 14.23 | -0.98 |
Eisosomes | 7.73 | 8.14 | 8.48 | 8.87 | 3.27 | 4.10 | 0.95 | 6.78 | 4.88 | 5.18 | 8.13 | 7.86 | 8.82 | 8.78 | 5.92 |
Endoplasmic Reticulum | 0.13 | -0.66 | 1.52 | 3.06 | 1.65 | 3.28 | 0.13 | 4.31 | 4.28 | 4.82 | 2.01 | -0.63 | 2.40 | 1.14 | 3.30 |
Endosome | -10.91 | -5.43 | -5.43 | 3.92 | 2.59 | 1.81 | 3.56 | 11.11 | 10.90 | 6.47 | -17.05 | -4.94 | 3.19 | 16.30 | 6.35 |
Golgi | 23.67* | 11.99 | 33.65* | 11.41 | 4.54 | -5.14 | 11.25 | 11.58 | 24.55 | 0.99 | 18.38 | 24.18 | 32.88 | 21.88 | 2.80 |
Lipid Particles | 4.17 | 3.30 | 13.46 | 8.36 | 3.17 | 4.52 | 2.52 | 5.48 | 2.47 | 1.54 | 8.30 | 4.48 | 14.63 | 9.00 | 3.21 |
Mitochondria | 6.26 | 17.46 | 16.03 | 8.59 | -1.26 | -5.58 | 3.97 | 4.41 | 12.96 | 2.50 | 4.86 | 17.84 | 15.54 | 12.57 | -1.41 |
Mitotic Spindle | -4.68 | -3.99 | -13.22 | -12.38 | -0.71 | -8.70 | -4.03 | -2.58 | -1.65 | -0.33 | -11.40 | -5.74 | -13.40 | -11.19 | -3.07 |
None | -2.85 | 4.72 | 3.99 | 3.50 | -4.22 | -0.49 | -0.94 | 1.34 | 1.71 | 1.75 | -2.33 | 0.66 | 4.11 | 3.82 | 1.48 |
Nuclear Periphery | -4.95 | -3.56 | -5.95 | -1.25 | 0.70 | 1.11 | -3.85 | 0.98 | -0.45 | 5.09 | -2.87 | -6.04 | -4.83 | -3.47 | 2.89 |
Nuclear Periphery Foci | -2.96 | -2.47 | -4.56 | -1.68 | 1.75 | 1.36 | -4.16 | -1.26 | -2.11 | 4.03 | -2.11 | -4.85 | -4.51 | -3.56 | 4.02 |
Nucleolus | -4.27 | -0.65 | -1.25 | 3.90 | 0.29 | -2.33 | -3.15 | 2.87 | 4.20 | 3.20 | -3.81 | -3.14 | -1.67 | 2.69 | 3.02 |
Nucleus | -5.53 | 1.28 | 0.18 | 5.83 | -1.27 | 2.59 | -3.99 | 0.74 | -1.02 | 4.07 | -2.71 | -3.78 | 0.08 | 2.93 | 3.79 |
Peroxisomes | 10.43 | -1.53 | 6.80 | -2.67 | 3.34 | 0.52 | -3.17 | -3.03 | -3.16 | -1.23 | 9.88 | -1.74 | 4.13 | -3.35 | 3.60 |
Vacuole | -18.10 | -6.05 | -25.82 | -16.08 | -8.24 | 1.60 | -7.90 | -4.79 | -5.10 | 0.49 | -15.16 | -10.89 | -24.83 | -20.04 | -4.15 |
Vacuole Periphery | -8.34 | -1.33 | -0.92 | 5.88 | 0.71 | -2.42 | 5.09 | 10.22 | 18.26 | 5.64 | -9.40 | 1.98 | 0.38 | 6.03 | -1.02 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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