Standard name | |
---|---|
Human Ortholog | |
Description | Ubiquitin-specific protease; removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 31 | 41 | 22 | 45 | 31 | 12 | 22 | 26 | 18 | – | 13 | 8 | 34 | 19 | 11 | 17 | 7 | 5 | 11 | 15 | 8 | 8 | 27 | 15 |
Bud | 11 | 18 | 8 | 12 | 13 | 15 | 18 | 17 | 21 | – | 13 | 20 | 2 | 6 | 4 | 3 | 2 | 0 | 3 | 4 | 1 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 10 | 9 | 5 | 4 | 11 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 32 | 47 | 50 | 45 | 44 | 35 | 29 | 27 | 17 | – | 21 | 30 | 86 | 122 | 92 | 86 | 64 | 54 | 16 | 18 | 25 | 28 | 74 | 79 |
Cytoplasm | 39 | 86 | 69 | 70 | 79 | 142 | 229 | 290 | 184 | – | 153 | 169 | 51 | 143 | 194 | 30 | 22 | 17 | 8 | 36 | 13 | 5 | 12 | 21 |
Endoplasmic Reticulum | 75 | 125 | 43 | 31 | 61 | 52 | 51 | 43 | 18 | – | 23 | 11 | 73 | 90 | 61 | 1 | 5 | 3 | 148 | 182 | 126 | 86 | 139 | 141 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 7 | 0 | – | 2 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 3 | 1 | 0 | 2 | 4 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 7 | 6 | 0 | 0 | 5 | 0 | 0 | 2 | 3 |
Mitochondria | 0 | 0 | 2 | 5 | 3 | 7 | 11 | 11 | 31 | – | 36 | 24 | 0 | 0 | 2 | 2 | 3 | 0 | 1 | 7 | 12 | 4 | 6 | 10 |
Nucleus | 1 | 3 | 1 | 0 | 0 | 0 | 6 | 3 | 1 | – | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | 5 | 1 | 2 | 5 | 5 | 20 | 3 | – | 3 | 9 | 0 | 1 | 0 | 4 | 0 | 0 | 4 | 19 | 11 | 5 | 7 | 7 |
Unique Cell Count | 155 | 254 | 159 | 154 | 179 | 233 | 308 | 384 | 250 | 225 | 224 | 178 | 275 | 271 | 130 | 91 | 64 | 201 | 309 | 213 | 149 | 281 | 306 | |
Labelled Cell Count | 190 | 322 | 201 | 211 | 234 | 269 | 373 | 445 | 294 | 268 | 274 | 246 | 381 | 366 | 152 | 110 | 79 | 201 | 309 | 213 | 149 | 281 | 306 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.6 | 10.8 | 8.8 | 8.7 | 8.6 | 6.8 | 6.6 | 7.0 | 6.0 | – | 5.4 | 5.9 | 9.5 | 9.0 | 8.0 | 18.3 | 17.6 | 18.6 | 9.3 | 8.9 | 8.8 |
Std Deviation (1e-4) | 1.7 | 1.8 | 1.0 | 1.2 | 1.1 | 1.0 | 1.0 | 1.3 | 1.1 | – | 1.0 | 0.9 | 1.3 | 1.1 | 1.4 | 3.4 | 3.0 | 2.9 | 1.2 | 1.3 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.02 | -0.03 | -0.39 | -0.43 | -0.34 | -0.55 | – | -0.71 | -0.58 | 0.1 | 0.03 | -0.13 | 1.05 | 1.0 | 1.07 | 0.08 | 0.01 | -0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 3.3 | 0.9 | -3.0 | -2.3 | -2.6 | -2.2 | – | -2.7 | -3.7 | 1.3 | -2.4 | -3.7 | -0.2 | -1.5 | -1.2 |
Bud | 1.0 | 0.8 | 0.6 | 0.4 | -0.3 | 1.3 | – | 0.3 | 1.4 | 0 | -1.6 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.4 | -1.4 | -3.9 | -6.0 | -7.4 | -6.6 | – | -5.5 | -4.3 | 3.2 | 2.7 | 0.5 | 5.9 | 5.9 | 7.2 |
Cytoplasm | 0.4 | 0.1 | 3.4 | 6.6 | 7.2 | 6.1 | – | 4.8 | 6.4 | -2.8 | 1.7 | 5.8 | -3.6 | -3.0 | -2.3 |
Endoplasmic Reticulum | -1.4 | 1.4 | -1.1 | -2.7 | -4.6 | -5.5 | – | -4.3 | -6.1 | 2.7 | 1.2 | -1.1 | -6.2 | -4.2 | -3.7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 4.0 | – | 4.8 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 1.0 | 0 | – | 0 | 0.4 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.5385 | 11.204 | 10.4568 | 9.351 | 9.4603 | 10.2885 | 9.1932 | 11.6478 | 9.9989 | 8.4623 | 9.1523 | 10.3557 | 7.6542 | 10.1389 | 8.9424 | 8.4269 | 5.6634 | 9.482 |
Actin | 0.0104 | 0.0005 | 0.0013 | 0.0004 | 0.0004 | 0.0004 | 0.0174 | 0.0003 | 0.0094 | 0.0169 | 0.0149 | 0.0005 | 0.0301 | 0.0003 | 0.0151 | 0.0133 | 0.0196 | 0.0002 |
Bud | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0004 | 0.0004 | 0.0002 | 0.0005 | 0 | 0.0002 | 0.0003 | 0.003 | 0 |
Bud Neck | 0.0006 | 0.0002 | 0.0004 | 0.0003 | 0.0005 | 0.0007 | 0.0009 | 0.0001 | 0.0004 | 0.0006 | 0.0006 | 0.0006 | 0.0013 | 0.0001 | 0.0002 | 0.0005 | 0.0005 | 0.0005 |
Bud Periphery | 0.0006 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0003 | 0 | 0.0005 | 0.0002 | 0.0006 | 0.0002 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0037 | 0 |
Bud Site | 0.001 | 0.0003 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0.0002 | 0.002 | 0.0008 | 0.0016 | 0.0002 | 0.0065 | 0.0001 | 0.0011 | 0.0017 | 0.0106 | 0 |
Cell Periphery | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0004 | 0 |
Cytoplasm | 0.8991 | 0.969 | 0.9277 | 0.8731 | 0.9407 | 0.9696 | 0.879 | 0.9931 | 0.9148 | 0.8576 | 0.9245 | 0.9676 | 0.8491 | 0.9924 | 0.9374 | 0.8887 | 0.6251 | 0.9774 |
Cytoplasmic Foci | 0.0138 | 0.0017 | 0.0025 | 0.0042 | 0.0047 | 0.0033 | 0.017 | 0.0012 | 0.0063 | 0.0175 | 0.0068 | 0.0023 | 0.0169 | 0.0009 | 0.0089 | 0.0207 | 0.0371 | 0.0019 |
Eisosomes | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 |
Endoplasmic Reticulum | 0.0222 | 0.0184 | 0.0459 | 0.0737 | 0.016 | 0.0151 | 0.0188 | 0.0026 | 0.0146 | 0.0087 | 0.0099 | 0.0177 | 0.0162 | 0.0035 | 0.0057 | 0.047 | 0.0124 | 0.0124 |
Endosome | 0.0091 | 0.0016 | 0.0033 | 0.0221 | 0.0281 | 0.0045 | 0.0266 | 0.0006 | 0.0172 | 0.0467 | 0.0183 | 0.0052 | 0.0188 | 0.0005 | 0.0089 | 0.0084 | 0.0542 | 0.0027 |
Golgi | 0.0017 | 0.0002 | 0.0004 | 0.0005 | 0.0007 | 0.0004 | 0.0065 | 0.0001 | 0.0032 | 0.0183 | 0.0077 | 0.0004 | 0.0057 | 0.0001 | 0.0039 | 0.0026 | 0.0224 | 0.0002 |
Lipid Particles | 0.0038 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0039 | 0.0001 | 0.0037 | 0.007 | 0.0021 | 0 | 0.0051 | 0 | 0.0019 | 0.0034 | 0.0699 | 0 |
Mitochondria | 0.0012 | 0.0001 | 0.0002 | 0.0008 | 0.0002 | 0.0006 | 0.0024 | 0 | 0.0071 | 0.0066 | 0.0051 | 0.0007 | 0.0089 | 0 | 0.0043 | 0.0007 | 0.0094 | 0.0001 |
None | 0.0168 | 0.0012 | 0.008 | 0.0027 | 0.0016 | 0.0011 | 0.0119 | 0.0006 | 0.0022 | 0.0022 | 0.0009 | 0.0005 | 0.0076 | 0.0009 | 0.0015 | 0.002 | 0.011 | 0.0018 |
Nuclear Periphery | 0.0039 | 0.0037 | 0.0026 | 0.0056 | 0.0015 | 0.001 | 0.0036 | 0.0003 | 0.0061 | 0.0061 | 0.0017 | 0.001 | 0.0089 | 0.0004 | 0.0013 | 0.0037 | 0.0076 | 0.0008 |
Nucleolus | 0.0003 | 0 | 0.0003 | 0 | 0 | 0 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0012 | 0 |
Nucleus | 0.0024 | 0.0015 | 0.0019 | 0.0021 | 0.0014 | 0.0014 | 0.002 | 0.0006 | 0.0024 | 0.0027 | 0.0015 | 0.0016 | 0.011 | 0.0006 | 0.001 | 0.0016 | 0.0038 | 0.0014 |
Peroxisomes | 0.0061 | 0 | 0.0001 | 0 | 0 | 0 | 0.0032 | 0 | 0.0042 | 0.0025 | 0.0005 | 0.0001 | 0.0036 | 0 | 0.0066 | 0.0018 | 0.0949 | 0 |
Punctate Nuclear | 0.0037 | 0.0002 | 0.0011 | 0.0002 | 0.0001 | 0.0001 | 0.0029 | 0 | 0.0012 | 0.0024 | 0.0006 | 0.0004 | 0.0052 | 0 | 0.0009 | 0.0026 | 0.0066 | 0.0001 |
Vacuole | 0.0017 | 0.001 | 0.0022 | 0.0113 | 0.0028 | 0.0012 | 0.0012 | 0.0002 | 0.0027 | 0.0016 | 0.0016 | 0.0005 | 0.002 | 0.0001 | 0.0005 | 0.0006 | 0.0045 | 0.0002 |
Vacuole Periphery | 0.0003 | 0.0001 | 0.0002 | 0.0023 | 0.0008 | 0.0003 | 0.0005 | 0 | 0.0012 | 0.0008 | 0.0005 | 0.0002 | 0.0008 | 0 | 0.0003 | 0.0002 | 0.0019 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 50.1754 | 42.2153 | 52.1089 | 49.2596 | 50.6307 | 49.9207 | 46.788 | 43.7671 | 41.7157 | 50.8167 |
Translational Efficiency | 0.7491 | 0.8111 | 0.6492 | 0.6862 | 0.5221 | 0.449 | 0.6544 | 0.5998 | 0.5771 | 0.5566 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1590 | 220 | 244 | 1355 | 1843 | 1553 | 1881 | 309 | 3433 | 1773 | 2125 | 1664 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1080.22 | 1349.37 | 1733.71 | 1481.46 | 1073.37 | 1142.96 | 1354.52 | 1543.64 | 1076.54 | 1168.57 | 1398.06 | 1493.01 |
Standard Deviation | 153.79 | 203.71 | 222.33 | 188.89 | 170.32 | 220.46 | 203.98 | 223.53 | 162.91 | 228.80 | 239.00 | 197.27 |
Intensity Change Log 2 | — | 0.320961 | 0.682537 | 0.455695 | — | 0.090627 | 0.335634 | 0.524189 | — | 0.210751 | 0.520037 | 0.490239 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000182 | 0.000537 | 0.001246 | 0.001136 | 0.000156 | 0.000657 | 0.000270 | 0.001429 | 0.000168 | 0.000642 | 0.000382 | 0.001190 |
Bud Neck | 0.000634 | 0.001465 | 0.009643 | 0.003397 | 0.000854 | 0.001016 | 0.001437 | 0.004560 | 0.000752 | 0.001072 | 0.002379 | 0.003613 |
Bud Site | 0.005657 | 0.015942 | 0.019182 | 0.019500 | 0.004884 | 0.012618 | 0.006368 | 0.029360 | 0.005242 | 0.013031 | 0.007839 | 0.021331 |
Cell Periphery | 0.000150 | 0.000308 | 0.001059 | 0.000534 | 0.000137 | 0.000185 | 0.000126 | 0.001039 | 0.000143 | 0.000200 | 0.000233 | 0.000628 |
Cytoplasm | 0.811596* | 0.742999* | 0.484008* | 0.742330* | 0.810616* | 0.796174* | 0.699704* | 0.635441* | 0.811070* | 0.789576* | 0.674937* | 0.722481* |
Cytoplasmic Foci | 0.097517 | 0.133241 | 0.056348 | 0.039811 | 0.088752 | 0.095549 | 0.036679 | 0.067358 | 0.092812 | 0.100226 | 0.038937 | 0.044926 |
Eisosomes | 0.000012 | 0.000015 | 0.000024 | 0.000016 | 0.000011 | 0.000014 | 0.000016 | 0.000037 | 0.000012 | 0.000015 | 0.000017 | 0.000020 |
Endoplasmic Reticulum | 0.011370 | 0.006428 | 0.002921 | 0.000877 | 0.007857 | 0.005239 | 0.002384 | 0.000821 | 0.009484 | 0.005387 | 0.002446 | 0.000866 |
Endosome | 0.003661 | 0.006740 | 0.014298 | 0.006650 | 0.004154 | 0.006455 | 0.004514 | 0.006885 | 0.003926 | 0.006490 | 0.005637 | 0.006694 |
Golgi | 0.003757 | 0.004369 | 0.001601 | 0.002844 | 0.003151 | 0.005654 | 0.000789 | 0.003491 | 0.003432 | 0.005495 | 0.000882 | 0.002964 |
Lipid Particles | 0.001751 | 0.005410 | 0.018884 | 0.014155 | 0.002600 | 0.003746 | 0.003692 | 0.034070 | 0.002207 | 0.003952 | 0.005436 | 0.017853 |
Mitochondria | 0.000656 | 0.000179 | 0.000095 | 0.002011 | 0.000246 | 0.001095 | 0.000212 | 0.005492 | 0.000436 | 0.000982 | 0.000198 | 0.002657 |
Mitotic Spindle | 0.001500 | 0.009568 | 0.008211 | 0.012114 | 0.000940 | 0.002230 | 0.002316 | 0.011522 | 0.001199 | 0.003140 | 0.002993 | 0.012004 |
None | 0.004934 | 0.000653 | 0.001777 | 0.001774 | 0.004487 | 0.003154 | 0.002807 | 0.001099 | 0.004694 | 0.002844 | 0.002688 | 0.001649 |
Nuclear Periphery | 0.000083 | 0.000105 | 0.001328 | 0.000146 | 0.000099 | 0.000095 | 0.000559 | 0.000155 | 0.000092 | 0.000096 | 0.000647 | 0.000148 |
Nuclear Periphery Foci | 0.002620 | 0.008392 | 0.032970 | 0.014015 | 0.003849 | 0.004786 | 0.010425 | 0.024358 | 0.003280 | 0.005233 | 0.013014 | 0.015935 |
Nucleolus | 0.000205 | 0.000145 | 0.000164 | 0.000212 | 0.000290 | 0.000433 | 0.000269 | 0.000107 | 0.000251 | 0.000397 | 0.000257 | 0.000193 |
Nucleus | 0.020507 | 0.012187 | 0.076956 | 0.022080 | 0.023069 | 0.016307 | 0.059024 | 0.016171 | 0.021883 | 0.015795 | 0.061083 | 0.020983 |
Peroxisomes | 0.000268 | 0.000881 | 0.000266 | 0.000762 | 0.000307 | 0.001111 | 0.000497 | 0.000865 | 0.000289 | 0.001082 | 0.000470 | 0.000781 |
Vacuole | 0.031211 | 0.049239 | 0.268512* | 0.113635 | 0.042257 | 0.041924 | 0.167285 | 0.155146 | 0.037141 | 0.042832 | 0.178909 | 0.121343 |
Vacuole Periphery | 0.001727 | 0.001199 | 0.000507 | 0.002001 | 0.001285 | 0.001557 | 0.000630 | 0.000593 | 0.001489 | 0.001513 | 0.000616 | 0.001740 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.91 | -10.17 | -4.90 | -2.70 | 1.48 | -2.68 | -3.57 | -7.38 | -2.10 | -6.38 | -2.87 | -7.16 | -6.10 | -2.07 | -4.47 |
Bud Neck | -5.18 | -13.74 | -13.33 | -7.05 | 10.23 | -1.78 | -7.12 | -11.06 | -10.51 | -8.14 | -4.35 | -13.77 | -15.85 | -13.52 | -2.86 |
Bud Site | -3.31 | -7.91 | -10.17 | -0.45 | 2.20 | -6.29 | -2.49 | -7.56 | -4.71 | -6.87 | -6.81 | -4.73 | -13.13 | -4.04 | -8.86 |
Cell Periphery | -3.87 | -10.41 | -9.65 | -2.81 | 7.18 | -3.44 | 1.47 | -7.32 | -6.61 | -7.47 | -4.27 | -6.71 | -12.29 | -9.42 | -7.85 |
Cytoplasm | 4.83 | 23.24 | 17.83 | 4.34 | -14.33 | 2.17 | 19.63 | 16.64 | 15.41 | 7.72 | 3.86 | 26.16 | 23.85 | 17.89 | -0.49 |
Cytoplasmic Foci | -3.59 | 7.59 | 15.72 | 9.82 | 3.47 | -1.57 | 15.85 | 3.77 | 4.84 | -6.40 | -1.96 | 20.72 | 17.64 | 15.62 | -2.43 |
Eisosomes | -1.72 | -9.92 | -3.16 | -0.67 | 6.49 | -2.43 | -4.31 | -7.69 | -5.90 | -6.09 | -2.48 | -6.50 | -6.49 | -2.93 | -1.87 |
Endoplasmic Reticulum | 2.33 | 5.95 | 7.30 | 2.86 | 6.62 | 1.87 | 5.30 | 6.57 | 5.28 | 7.14 | 3.47 | 8.18 | 9.79 | 5.83 | 8.23 |
Endosome | -2.97 | -8.78 | -1.18 | 1.89 | 7.22 | -3.63 | 0.05 | -0.71 | 1.79 | -0.73 | -4.63 | -3.93 | -1.02 | 2.72 | 1.23 |
Golgi | -0.14 | 6.93 | 5.68 | 1.77 | 0.18 | -3.28 | 9.05 | 2.91 | 4.60 | -1.22 | -2.90 | 11.11 | 6.51 | 5.71 | -2.26 |
Lipid Particles | -4.10 | -10.07 | -12.39 | -6.70 | 3.30 | -1.78 | -4.74 | -7.07 | -6.63 | -6.61 | -3.02 | -10.45 | -13.72 | -10.63 | -9.47 |
Mitochondria | 2.14 | 2.62 | -0.28 | -1.06 | -1.16 | -1.90 | 1.29 | -0.88 | -0.34 | -0.93 | -1.35 | 2.56 | -0.94 | -0.03 | -1.43 |
Mitotic Spindle | -1.90 | -6.24 | -4.17 | 0.52 | 1.69 | -1.65 | -2.80 | -1.41 | -0.89 | -0.65 | -2.32 | -4.25 | -4.68 | -2.67 | -2.50 |
None | 5.97 | 5.12 | 4.81 | -1.41 | 0.18 | 1.39 | 2.43 | 4.24 | 4.45 | 2.31 | 2.70 | 3.77 | 5.80 | 3.46 | 1.72 |
Nuclear Periphery | -1.55 | -13.75 | -8.22 | -4.32 | 12.49 | 0.13 | -5.16 | -6.11 | -5.88 | 3.56 | -0.55 | -7.60 | -10.88 | -8.93 | 5.67 |
Nuclear Periphery Foci | -5.49 | -12.56 | -13.84 | -4.74 | 7.34 | -2.44 | -10.86 | -8.26 | -7.75 | -4.89 | -5.61 | -15.94 | -15.45 | -12.19 | -2.16 |
Nucleolus | 1.78 | 0.29 | -0.36 | -2.14 | -0.57 | -1.98 | 0.99 | 6.50 | 4.27 | 4.92 | -2.29 | 0.21 | 2.93 | 3.09 | 1.88 |
Nucleus | 1.07 | -9.99 | -5.36 | -2.81 | 8.70 | 5.55 | -15.70 | 3.65 | -0.22 | 16.01 | 5.09 | -18.97 | -3.42 | -7.19 | 16.23 |
Peroxisomes | -2.98 | -1.89 | -3.87 | 1.34 | -3.10 | -2.68 | -0.75 | -3.98 | 1.68 | -1.14 | -2.97 | -0.90 | -4.47 | 1.91 | -1.12 |
Vacuole | -4.10 | -17.29 | -28.92 | -18.33 | 7.73 | -0.05 | -28.98 | -16.88 | -16.86 | -5.36 | -3.27 | -34.34 | -32.42 | -30.20 | 0.57 |
Vacuole Periphery | 1.12 | 5.35 | 2.02 | 0.35 | -1.48 | -1.08 | 3.68 | 6.59 | 5.74 | 1.01 | 0.02 | 4.90 | 2.38 | 2.15 | -1.04 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|