Standard name
Human Ortholog
Description Protein of unknown function; localized to the vacuolar outer membrane; predicted to be palmitoylated
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0.06 0.1 0.13 – 0.17 0.21 0.24 0.27 0.31 0.06 0 0 0.12 0.06 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.2 0 0.09 – 0.05 0.1 0.11 0.11 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.11 0.14 0.08 0 0 0.07 – 0 0 0 0 0 0 0 0 0.12 0.1 0.08 0.07 0.06 0 0.05 0.07 0.08
Nuclear Periphery 0.06 0.07 0.13 0.22 0.06 0.15 – 0.21 0.19 0.13 0.12 0.06 0.16 0.21 0.18 0.06 0.05 0.1 0 0 0.07 0 0 0
Nucleolus 0.08 0.05 0.07 0.09 0.05 0 – 0 0 0 0 0 0.07 0.05 0 0.08 0.13 0.07 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.79 0.85 0.79 0.68 0.79 0.64 – 0.64 0.6 0.54 0.54 0.5 0.8 0.89 0.89 0.74 0.81 0.76 0.82 0.9 0.81 0.82 0.79 0.76
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 – 4 4 1 0 7 0 0 1 0 0 0 0 0 1 0 0 1
Bud 0 0 1 0 0 2 – 0 1 1 1 6 0 0 0 1 0 0 0 0 2 1 3 10
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 3 4 4 – 16 10 13 16 20 1 0 2 2 1 0 0 0 0 0 0 0
Cytoplasm 7 0 9 15 28 53 – 82 69 77 85 110 22 19 18 14 6 15 1 0 2 0 1 3
Endoplasmic Reticulum 0 0 1 1 1 7 – 16 7 14 17 16 3 2 0 2 2 3 0 0 0 0 1 3
Endosome 0 0 7 2 7 6 – 4 0 1 0 2 8 3 7 0 1 1 4 0 11 3 5 16
Golgi 1 1 2 2 6 4 – 8 5 1 0 3 6 1 7 5 1 7 0 0 1 0 1 1
Mitochondria 6 2 27 48 11 37 – 24 32 35 34 46 5 8 2 1 3 4 0 0 2 3 1 3
Nucleus 16 13 26 2 11 27 – 13 8 13 6 8 17 9 18 14 9 8 10 6 10 12 23 37
Nuclear Periphery 9 6 43 52 18 60 – 105 63 43 39 23 65 103 99 7 5 10 5 1 25 7 15 15
Nucleolus 12 5 23 20 13 15 – 8 7 5 2 3 29 25 22 9 12 7 5 1 4 9 11 13
Peroxisomes 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 2 – 0 1 0 0 0 1 1 0 0 1 0 0 0 0 1 2 2
Vac/Vac Membrane 114 78 255 160 225 264 – 315 198 172 170 177 320 426 490 83 76 74 125 107 284 199 268 349
Unique Cell Count 145 92 321 235 284 410 491 331 321 316 355 400 480 551 112 94 98 154 120 349 242 340 460
Labelled Cell Count 165 105 394 305 326 483 595 405 376 370 421 477 597 666 139 118 129 154 120 349 242 340 460


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.3 21.4 12.7 15.3 15.4 13.7 – 12.7 12.3 12.0 11.5 11.8 18.4 19.0 17.9 41.6 34.9 33.3 18.5 21.1 19.9
Std Deviation (1e-4) 4.8 6.4 4.0 4.4 4.7 4.5 – 3.8 3.8 3.5 3.5 3.6 5.9 5.3 5.0 20.2 17.9 18.6 6.8 10.0 7.0
Intensity Change (Log2) – – – 0.27 0.28 0.12 – 0.0 -0.05 -0.09 -0.14 -0.1 0.53 0.58 0.5 1.71 1.46 1.39 0.55 0.73 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 3.3 0 3.6 4.1 4.3 0 0 0 0 0 0
Cytoplasm 2.1 3.6 4.9 – 6.1 7.1 7.9 8.6 9.6 1.8 0.9 0.4 3.9 0 4.7
Endoplasmic Reticulum 0 0 0 – 2.9 0 3.4 3.9 3.5 0 0 0 0 0 0
Endosome 0 0.2 -0.7 – 0 0 0 0 0 -0.2 0 -1.0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.1 -2.3 0.3 – -2.0 0.6 1.1 1.0 1.9 -4.6 -4.6 -6.4 -2.8 -1.7 -1.4
Nucleus -3.9 -2.2 -0.8 – -3.6 -3.3 -2.1 -3.6 -3.5 -2.2 -4.2 -3.1 1.4 0.5 0
Nuclear Periphery 2.7 -2.9 0.5 – 2.9 2.0 0 -0.4 -3.0 1.1 2.9 1.8 -2.0 -2.2 -0.8
Nucleolus 0.6 -1.3 -2.1 – -4.0 -3.1 -3.5 -4.2 -4.3 0 -1.1 -2.0 0.3 1.7 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.0 -0.1 -4.4 – -4.7 -5.4 -6.9 -6.9 -8.0 0.2 3.6 3.8 -1.2 0.3 -0.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.6545 18.7678 15.5578 13.663 12.5948 16.0421 12.1971 17.5463 15.6705 14.9453 13.5935 16.2214 15.8118 20.411 17.9169 16.2003 17.6672 18.0074
Actin 0.0106 0 0.0024 0 0.0054 0.0001 0.0171 0 0.0015 0.0003 0.0022 0.0035 0.0031 0 0.0078 0.026 0 0.0001
Bud 0.0003 0 0.0001 0.0001 0.001 0 0.0003 0 0.0001 0.0001 0 0.0001 0.0002 0 0.0001 0.0002 0 0
Bud Neck 0.0005 0.0001 0.0001 0 0.0001 0 0.0004 0 0.0001 0 0.0001 0.0001 0.0003 0 0.0001 0.0005 0 0
Bud Periphery 0.0005 0.0001 0.0002 0.0003 0.0036 0.0001 0.0005 0.0001 0.0002 0.0003 0.0001 0.0004 0.0005 0.0001 0.0002 0.0004 0.0002 0.0002
Bud Site 0.0026 0 0.0003 0.0001 0.0004 0 0.0014 0 0.0001 0.0001 0.0002 0 0.0008 0 0.0001 0.0011 0 0.0001
Cell Periphery 0.0012 0.0008 0.0008 0.0008 0.0005 0.0004 0.0016 0.0007 0.001 0.0008 0.0002 0.0003 0.0015 0.001 0.001 0.0007 0.0009 0.0003
Cytoplasm 0.0017 0.0002 0.0003 0.0018 0.0129 0.0002 0.011 0.0002 0.0006 0 0.0001 0.0001 0.0022 0.0002 0.005 0.0006 0.0077 0.0001
Cytoplasmic Foci 0.0089 0.0003 0.0043 0.0042 0.0078 0.0011 0.0124 0.0004 0.0031 0.0007 0.0041 0.0003 0.0099 0.0001 0.0008 0.0128 0.0088 0.0001
Eisosomes 0.0002 0 0.0001 0 0.0001 0 0.0003 0 0 0 0 0 0 0 0 0.0002 0 0
Endoplasmic Reticulum 0.0011 0.0002 0.0003 0.0005 0.012 0.0003 0.0022 0.0001 0.0006 0.0001 0.0001 0.0002 0.0021 0.0001 0.0004 0.0004 0.0004 0.0001
Endosome 0.016 0.0028 0.0077 0.0629 0.1041 0.008 0.0201 0.0017 0.0088 0.0024 0.0048 0.0023 0.0141 0.0017 0.0074 0.0149 0.0089 0.0028
Golgi 0.0093 0.0002 0.0036 0.0013 0.0205 0.0015 0.0068 0.0001 0.0013 0.0005 0.0061 0.0014 0.0028 0.0001 0.0009 0.006 0.0002 0.0004
Lipid Particles 0.0191 0.0029 0.002 0.0011 0.0066 0.0005 0.0117 0.002 0.0012 0.0104 0.025 0.0025 0.0162 0.0012 0.0089 0.0145 0.0003 0.0014
Mitochondria 0.0216 0.0015 0.0035 0.0003 0.0136 0.0016 0.0118 0.0007 0.0029 0.0113 0.0133 0.0008 0.0062 0.0026 0.0027 0.0166 0.0002 0.0159
None 0.0006 0 0.0001 0.0003 0.003 0 0.0087 0 0.0001 0 0 0 0.0012 0 0.0001 0.0004 0.002 0
Nuclear Periphery 0.0171 0.009 0.0049 0.0067 0.0081 0.0046 0.0052 0.0071 0.0142 0.0013 0.018 0.0182 0.0097 0.0126 0.0113 0.001 0.0068 0.0083
Nucleolus 0.0016 0.0012 0.0012 0.0005 0.0001 0.001 0.0116 0.0042 0.001 0.0007 0.0002 0.0002 0.0028 0.0013 0.0021 0.0008 0.0002 0.0018
Nucleus 0.0048 0.0062 0.0011 0.0026 0.001 0.0007 0.0076 0.0026 0.0021 0.0006 0.0002 0.0008 0.0034 0.0024 0.0083 0.0008 0.0011 0.0204
Peroxisomes 0.0087 0.0001 0.0003 0.0001 0.0004 0.0001 0.0076 0 0.0009 0.0009 0.009 0.0005 0.0071 0 0.0003 0.0227 0.0002 0.0001
Punctate Nuclear 0.0018 0 0.0002 0.0018 0.0004 0 0.0047 0 0.0002 0 0.0001 0.0001 0.0073 0 0 0.0015 0.0082 0.0001
Vacuole 0.2303 0.2236 0.2214 0.2472 0.1439 0.1332 0.2502 0.2682 0.2631 0.2637 0.1783 0.16 0.2561 0.2566 0.2616 0.2914 0.2847 0.16
Vacuole Periphery 0.6415 0.7509 0.7452 0.6674 0.6543 0.8465 0.6067 0.7119 0.6968 0.7056 0.738 0.808 0.6523 0.72 0.6809 0.5864 0.6692 0.7878

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.1724 32.646 24.2248 26.9505 37.2027 26.0708 23.5774 14.3218 26.1656 25.5175
Translational Efficiency 0.9283 0.611 0.8574 1.2157 0.4691 1.0924 0.8109 1.6294 1.0892 0.7458

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1042 1469 140 651 820 2014 3162 1618 1862 3483 3302 2269

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1262.39 1484.52 1946.67 1891.05 1159.88 1434.66 1757.34 1828.39 1217.25 1455.69 1765.37 1846.37
Standard Deviation 299.35 376.80 340.10 407.07 283.48 288.07 314.83 400.19 296.86 329.35 318.24 403.17
Intensity Change Log 2 — 0.233839 0.624851 0.583030 — 0.306733 0.599418 0.656598 — 0.269203 0.612728 0.618726

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000009 0.000075 0.000184 0.000101 0.000066 0.000047 0.000027 0.000074 0.000034 0.000059 0.000034 0.000082
Bud Neck 0.000401 0.000439 0.003588 0.002525 0.000287 0.000482 0.000976 0.000912 0.000351 0.000464 0.001087 0.001375
Bud Site 0.002821 0.001686 0.002863 0.002878 0.006530 0.000562 0.000613 0.002718 0.004454 0.001036 0.000708 0.002764
Cell Periphery 0.001834 0.001096 0.000109 0.000549 0.002709 0.000976 0.001266 0.001551 0.002219 0.001027 0.001217 0.001263
Cytoplasm 0.002643 0.001339 0.000192 0.000136 0.007354 0.000162 0.000044 0.000234 0.004718 0.000659 0.000050 0.000206
Cytoplasmic Foci 0.001030 0.001289 0.011001 0.000655 0.001682 0.001038 0.000579 0.001493 0.001317 0.001144 0.001021 0.001252
Eisosomes 0.000003 0.000011 0.000063 0.000029 0.000006 0.000029 0.000146 0.000028 0.000004 0.000021 0.000142 0.000028
Endoplasmic Reticulum 0.000686 0.000447 0.000145 0.000229 0.001239 0.000722 0.000376 0.000418 0.000929 0.000606 0.000366 0.000363
Endosome 0.000896 0.001764 0.003582 0.002160 0.002818 0.001765 0.000753 0.003966 0.001743 0.001764 0.000873 0.003448
Golgi 0.001166 0.001960 0.002010 0.005080 0.004508 0.000997 0.000473 0.008974 0.002638 0.001403 0.000538 0.007857
Lipid Particles 0.000128 0.000507 0.000467 0.001500 0.000265 0.000977 0.001752 0.001621 0.000188 0.000779 0.001698 0.001586
Mitochondria 0.000378 0.002538 0.013725 0.008898 0.005478 0.006015 0.003020 0.008286 0.002624 0.004548 0.003474 0.008461
Mitotic Spindle 0.000020 0.000438 0.001171 0.000729 0.001102 0.000128 0.000101 0.000318 0.000497 0.000259 0.000147 0.000436
None 0.000440 0.000383 0.000495 0.000286 0.000953 0.000360 0.000257 0.000329 0.000666 0.000370 0.000267 0.000317
Nuclear Periphery 0.000052 0.000462 0.012558 0.003553 0.000098 0.001087 0.001362 0.001562 0.000072 0.000823 0.001837 0.002133
Nuclear Periphery Foci 0.000053 0.000568 0.006154 0.002905 0.001304 0.000665 0.000606 0.001021 0.000604 0.000624 0.000841 0.001562
Nucleolus 0.000364 0.001694 0.036150 0.004656 0.000792 0.003471 0.006623 0.002226 0.000552 0.002722 0.007875 0.002924
Nucleus 0.000593 0.001625 0.003777 0.001271 0.003975 0.002182 0.003142 0.000697 0.002083 0.001947 0.003169 0.000862
Peroxisomes 0.000057 0.000184 0.002388 0.000160 0.000656 0.000207 0.000106 0.000073 0.000320 0.000198 0.000203 0.000098
Vacuole 0.177721 0.210604* 0.192798 0.400150* 0.078529 0.282872* 0.440454* 0.388929* 0.134038 0.252392* 0.429953* 0.392149*
Vacuole Periphery 0.808705* 0.770891* 0.706580* 0.561549* 0.879649* 0.695254* 0.537325* 0.574569* 0.839948* 0.727155* 0.544501* 0.570833*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.64 -4.68 -2.63 -0.66 1.54 0.76 1.81 -0.31 -1.34 -2.86 -1.59 0.05 -2.66 -1.19 -3.10
Bud Neck -0.18 -4.35 -3.64 -3.66 0.70 -1.92 -5.13 -3.67 -2.61 0.30 -0.91 -4.78 -4.65 -4.48 -1.62
Bud Site 1.36 -0.08 -0.30 -1.55 -0.11 4.22 4.17 2.42 -3.44 -3.34 4.48 4.99 1.74 -3.37 -4.11
Cell Periphery 3.05 8.56 5.95 3.51 -5.53 3.35 2.82 2.18 -2.76 -1.47 4.52 3.80 3.44 -1.55 -0.31
Cytoplasm 1.27 2.95 3.03 2.04 0.61 4.14 4.20 4.06 -1.01 -2.19 4.39 5.19 4.95 1.54 -2.43
Cytoplasmic Foci -0.30 -1.71 0.46 1.59 1.79 1.60 2.26 1.03 -0.94 -2.10 0.72 0.85 0.56 -0.28 -0.51
Eisosomes -5.10 -2.18 -7.75 -4.82 1.22 -6.17 -14.67 -6.99 0.25 12.34 -8.19 -15.37 -11.51 -2.66 12.45
Endoplasmic Reticulum 2.13 5.96 5.53 2.49 -1.50 2.47 4.66 4.34 2.85 -0.89 2.84 6.01 5.80 3.29 0.06
Endosome -2.62 -1.51 -2.68 -0.75 0.80 1.35 2.78 -1.16 -3.10 -4.81 -0.06 2.36 -2.90 -3.21 -5.07
Golgi -2.11 -1.47 -3.45 -2.64 -2.43 4.07 4.72 -2.91 -6.09 -6.51 2.87 5.03 -4.98 -6.47 -7.38
Lipid Particles -6.16 -3.65 -3.82 -2.72 -2.77 -5.55 -13.78 -6.27 -2.94 0.63 -8.26 -20.63 -7.82 -4.37 0.60
Mitochondria -3.46 -3.93 -5.47 -3.80 1.29 -0.36 1.87 -1.64 -1.51 -4.21 -2.37 -1.26 -5.28 -3.47 -4.81
Mitotic Spindle -1.37 -2.32 -1.20 -0.44 0.56 0.98 1.00 0.82 -1.46 -1.75 0.60 0.82 0.26 -0.78 -1.47
None 0.50 -0.47 1.57 1.47 2.97 2.22 2.64 2.33 0.53 -1.63 2.23 3.12 2.64 1.12 -1.51
Nuclear Periphery -5.19 -3.32 -3.71 -3.27 2.32 -5.83 -5.73 -5.55 -1.52 -0.58 -7.28 -6.61 -6.25 -3.80 -0.70
Nuclear Periphery Foci -2.48 -2.70 -2.64 -2.12 1.30 0.87 0.94 0.49 -1.94 -2.43 0.14 -0.36 -1.65 -2.70 -2.06
Nucleolus -3.90 -4.04 -6.02 -3.78 3.53 -5.13 -10.62 -2.82 2.13 7.20 -6.89 -12.14 -6.30 -0.48 7.19
Nucleus -2.23 -2.83 -2.88 0.58 2.13 0.98 0.47 1.83 3.47 7.85 0.15 -1.30 1.49 3.25 7.95
Peroxisomes -1.86 -2.99 -1.94 0.31 2.86 0.98 1.13 1.18 2.25 1.92 0.73 0.71 1.06 2.20 2.69
Vacuole -4.15 -0.98 -20.46 -17.89 -12.19 -28.47 -55.19* -41.46 -13.33 6.87 -21.05 -48.83 -40.54 -22.27 5.57
Vacuole Periphery 4.32 4.33 21.25 18.52 5.87 19.29* 38.60* 31.20* 14.21 -4.69 17.18 43.37 37.21 23.29 -3.63
Standard name
Human Ortholog
Description Protein of unknown function; localized to the vacuolar outer membrane; predicted to be palmitoylated
Localization
Cell Percentages vacuolar membrane (84%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

Sna4

Sna4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sna4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available