Standard name | |
---|---|
Human Ortholog | |
Description | Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity; involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 3 | 15 | 28 | 33 | 25 | 19 | 23 | 13 | 17 | 10 | 24 | 4 | 7 | 5 | – | 0 | 0 | 0 | 1 | 11 | 5 | 14 | 13 |
Bud | 1 | 0 | 1 | 5 | 5 | 9 | 14 | 21 | 22 | 20 | 30 | 35 | 1 | 0 | 0 | – | 1 | 0 | 2 | 0 | 14 | 8 | 13 | 12 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | – | 0 | 0 | 1 | 0 | 2 | 2 | 2 | 4 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 7 | 4 | 16 | 23 | 25 | 12 | 16 | 13 | 11 | 24 | 18 | 26 | 19 | – | 3 | 3 | 0 | 0 | 1 | 3 | 9 | 6 |
Cytoplasm | 46 | 19 | 112 | 129 | 215 | 243 | 403 | 478 | 345 | 501 | 331 | 469 | 71 | 70 | 90 | – | 26 | 13 | 19 | 11 | 13 | 19 | 19 | 26 |
Endoplasmic Reticulum | 41 | 23 | 289 | 208 | 318 | 165 | 142 | 168 | 74 | 64 | 35 | 79 | 224 | 185 | 150 | – | 0 | 0 | 70 | 66 | 389 | 307 | 390 | 430 |
Endosome | 2 | 0 | 5 | 10 | 6 | 6 | 15 | 26 | 7 | 11 | 11 | 12 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 |
Golgi | 0 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 |
Mitochondria | 3 | 3 | 5 | 5 | 1 | 5 | 9 | 26 | 22 | 44 | 34 | 50 | 0 | 0 | 0 | – | 3 | 0 | 4 | 5 | 8 | 12 | 16 | 10 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 1 | 2 | 0 | 3 | 1 | 3 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 11 | 3 | 4 | 3 | 3 | 20 | 3 | 4 | 2 | 6 | 1 | 1 | 0 | – | 0 | 0 | 1 | 0 | 2 | 2 | 2 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 4 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 6 | 1 | 21 | 28 | 63 | 55 | 103 | 137 | 37 | 68 | 36 | 83 | 9 | 3 | 6 | – | 2 | 2 | 10 | 9 | 34 | 16 | 19 | 34 |
Unique Cell Count | 94 | 44 | 418 | 372 | 573 | 459 | 647 | 828 | 496 | 681 | 444 | 686 | 306 | 265 | 251 | 32 | 17 | 113 | 99 | 485 | 383 | 498 | 547 | |
Labelled Cell Count | 102 | 49 | 467 | 423 | 662 | 536 | 735 | 921 | 540 | 747 | 503 | 787 | 330 | 295 | 273 | 35 | 19 | 113 | 99 | 485 | 383 | 498 | 547 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 7.5 | 8.3 | 8.8 | 9.1 | 7.7 | 7.4 | 7.1 | 6.8 | 6.3 | 6.4 | 6.5 | 10.5 | 10.0 | 9.4 | – | 8.4 | 10.4 | 11.0 | 10.6 | 10.5 |
Std Deviation (1e-4) | 2.3 | 2.2 | 2.4 | 2.6 | 2.2 | 1.7 | 1.6 | 1.5 | 1.4 | 1.4 | 1.3 | 1.4 | 2.4 | 2.3 | 2.1 | – | 1.3 | 1.5 | 2.9 | 2.5 | 2.5 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.13 | -0.11 | -0.16 | -0.23 | -0.29 | -0.4 | -0.38 | -0.36 | 0.33 | 0.27 | 0.18 | – | 0.02 | 0.33 | 0.41 | 0.36 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 2.4 | 1.6 | 1.3 | -0.6 | -0.8 | -0.8 | -1.0 | -1.2 | -0.1 | -1.9 | -0.7 | -1.2 | – | 0 | 0 |
Bud | 0 | 0 | 0 | 2.6 | 2.9 | 4.0 | 3.2 | 5.1 | 4.4 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | -0.7 | 1.2 | 2.7 | 2.0 | -0.3 | 1.5 | 0.3 | 0.8 | 1.8 | 3.1 | 4.8 | 3.8 | – | 0 | 0 |
Cytoplasm | 2.4 | 3.5 | 7.9 | 11.3 | 10.3 | 12.9 | 15.2 | 14.0 | 13.4 | -1.1 | -0.1 | 2.5 | – | 6.4 | 0 |
Endoplasmic Reticulum | -3.8 | -4.4 | -9.8 | -15.3 | -16.9 | -16.7 | -20.6 | -18.6 | -19.7 | 1.2 | 0.2 | -2.5 | – | -7.9 | -5.9 |
Endosome | 1.5 | 0 | 0.1 | 1.3 | 2.1 | 0.3 | 0.6 | 1.4 | 0.7 | 0 | 0 | 0 | – | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0.3 | 2.1 | 2.9 | 4.1 | 4.6 | 4.5 | 0 | 0 | 0 | – | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | -1.9 | -2.5 | -2.3 | -3.0 | -0.2 | -2.5 | -2.8 | -2.6 | -2.3 | -2.4 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | 1.5 | 3.3 | 3.7 | 5.4 | 5.8 | 1.5 | 2.9 | 1.8 | 3.9 | -1.4 | -2.7 | -1.7 | – | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.4273 | 9.4404 | 9.0753 | 9.1634 | 6.7997 | 10.3259 | 8.1086 | 9.4565 | 8.5161 | 7.5906 | 6.9139 | 9.3219 | 7.2849 | 8.6475 | 9.0235 | 7.5528 | 5.7594 | 8.2571 |
Actin | 0.0245 | 0.0021 | 0.0017 | 0.0013 | 0.0622 | 0.0017 | 0.0109 | 0.0025 | 0.0002 | 0.0097 | 0.0006 | 0.0013 | 0.0534 | 0.0045 | 0.0002 | 0.0148 | 0.0029 | 0.0007 |
Bud | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0026 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0.0002 | 0.0002 | 0 |
Bud Neck | 0.0003 | 0.0006 | 0.0004 | 0.0003 | 0.0024 | 0.0006 | 0.0006 | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0011 | 0.0001 | 0.0001 | 0.0003 | 0.0014 | 0.0007 |
Bud Periphery | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0061 | 0.0001 | 0 | 0 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0005 | 0 |
Bud Site | 0.0003 | 0.0048 | 0.0007 | 0.0001 | 0.0008 | 0.0001 | 0.0016 | 0.0477 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0019 | 0 | 0 | 0.0005 | 0.0003 | 0 |
Cell Periphery | 0.0004 | 0.0068 | 0.0008 | 0.0007 | 0.0006 | 0.0004 | 0.001 | 0.001 | 0.001 | 0.001 | 0.0003 | 0.0006 | 0.0005 | 0.0005 | 0.0027 | 0.0007 | 0.0079 | 0.0008 |
Cytoplasm | 0.0476 | 0.1237 | 0.0796 | 0.0547 | 0.0328 | 0.0483 | 0.0494 | 0.0287 | 0.034 | 0.0166 | 0.0101 | 0.0123 | 0.0088 | 0.0242 | 0.0336 | 0.0281 | 0.1567 | 0.0486 |
Cytoplasmic Foci | 0.0046 | 0.0026 | 0.006 | 0.0037 | 0.0044 | 0.0015 | 0.0148 | 0.0008 | 0.0019 | 0.0057 | 0.0261 | 0.0013 | 0.0391 | 0.0017 | 0.0004 | 0.0043 | 0.0091 | 0.0037 |
Eisosomes | 0.0002 | 0.0002 | 0 | 0.0001 | 0.001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.8225 | 0.7734 | 0.8347 | 0.8328 | 0.6906 | 0.8722 | 0.7598 | 0.8848 | 0.9225 | 0.9122 | 0.9041 | 0.9243 | 0.7936 | 0.8874 | 0.9377 | 0.8987 | 0.7097 | 0.9074 |
Endosome | 0.02 | 0.0084 | 0.0223 | 0.0459 | 0.0517 | 0.0157 | 0.0325 | 0.0016 | 0.0108 | 0.009 | 0.0096 | 0.0106 | 0.0143 | 0.0095 | 0.0037 | 0.0125 | 0.043 | 0.0058 |
Golgi | 0.0031 | 0.0007 | 0.0013 | 0.001 | 0.0161 | 0.0011 | 0.0104 | 0.0001 | 0.0004 | 0.0253 | 0.0017 | 0.0016 | 0.0088 | 0.0071 | 0.0003 | 0.0021 | 0.0036 | 0.0006 |
Lipid Particles | 0.0066 | 0.0126 | 0.0084 | 0.0036 | 0.0311 | 0.0018 | 0.0186 | 0.0003 | 0.0051 | 0.0044 | 0.0334 | 0.0016 | 0.0129 | 0.0037 | 0.0042 | 0.006 | 0.016 | 0.0049 |
Mitochondria | 0.0011 | 0.0002 | 0.0005 | 0.0004 | 0.0149 | 0.0006 | 0.0032 | 0.0001 | 0 | 0.0004 | 0.0002 | 0.0124 | 0.0016 | 0.0001 | 0.0001 | 0.0008 | 0.0008 | 0.0001 |
None | 0.0044 | 0.0061 | 0.0016 | 0.001 | 0.0036 | 0.0025 | 0.0064 | 0.0119 | 0.0003 | 0.0002 | 0.0005 | 0.0004 | 0.0046 | 0.0002 | 0.0001 | 0.013 | 0.0021 | 0.002 |
Nuclear Periphery | 0.04 | 0.0176 | 0.0204 | 0.0384 | 0.0483 | 0.0307 | 0.0572 | 0.0057 | 0.0137 | 0.0085 | 0.0081 | 0.0219 | 0.0126 | 0.042 | 0.0086 | 0.0097 | 0.0142 | 0.0069 |
Nucleolus | 0.0003 | 0.0003 | 0.0003 | 0 | 0.0015 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 |
Nucleus | 0.0068 | 0.004 | 0.0057 | 0.0039 | 0.0066 | 0.0042 | 0.0047 | 0.001 | 0.0012 | 0.0004 | 0.0003 | 0.0011 | 0.0009 | 0.0049 | 0.0006 | 0.0014 | 0.0024 | 0.0007 |
Peroxisomes | 0.0052 | 0 | 0.0003 | 0 | 0.0014 | 0.0002 | 0.009 | 0.0001 | 0 | 0.0001 | 0.0023 | 0.0001 | 0.0279 | 0 | 0 | 0.0004 | 0.0002 | 0.0011 |
Punctate Nuclear | 0.0054 | 0.0005 | 0.0026 | 0.0006 | 0.0096 | 0.0019 | 0.0034 | 0.0003 | 0.0006 | 0.0001 | 0.0004 | 0.0002 | 0.0138 | 0.0002 | 0.0001 | 0.0017 | 0.0007 | 0.0057 |
Vacuole | 0.0051 | 0.033 | 0.0113 | 0.0101 | 0.0157 | 0.0148 | 0.0134 | 0.0039 | 0.0073 | 0.0048 | 0.0017 | 0.0084 | 0.0022 | 0.0127 | 0.0072 | 0.0037 | 0.0265 | 0.0099 |
Vacuole Periphery | 0.0013 | 0.0012 | 0.0011 | 0.0013 | 0.0038 | 0.0014 | 0.0023 | 0.0002 | 0.0004 | 0.0008 | 0.0002 | 0.0012 | 0.0004 | 0.001 | 0.0004 | 0.0006 | 0.0018 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 41.0652 | 36.7098 | 51.5196 | 71.3006 | 48.0343 | 43.7192 | 39.7396 | 43.0235 | 65.0995 | 60.2287 |
Translational Efficiency | 0.9799 | 1.0818 | 1.1056 | 0.9351 | 0.9154 | 1.3397 | 1.2794 | 1.0144 | 1.1405 | 0.9948 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
78 | 342 | 388 | 1007 | 150 | 797 | 341 | 1088 | 228 | 1139 | 729 | 2095 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1204.23 | 866.43 | 1240.86 | 1483.81 | 851.19 | 866.18 | 1484.51 | 1523.41 | 971.97 | 866.26 | 1354.83 | 1504.38 |
Standard Deviation | 224.55 | 168.77 | 257.31 | 424.20 | 152.53 | 164.20 | 360.71 | 423.82 | 246.19 | 165.59 | 332.98 | 424.46 |
Intensity Change Log 2 | — | -0.474956 | 0.043229 | 0.301195 | — | 0.025186 | 0.802434 | 0.839751 | — | -0.246486 | 0.407019 | 0.548997 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002018 | 0.001224 | 0.001528 | 0.004671 | 0.000673 | 0.000978 | 0.004663 | 0.003128 | 0.001133 | 0.001052 | 0.002994 | 0.003870 |
Bud Neck | 0.001652 | 0.004839 | 0.002589 | 0.006227 | 0.009172 | 0.013670 | 0.003331 | 0.004513 | 0.006599 | 0.011018 | 0.002936 | 0.005337 |
Bud Site | 0.008769 | 0.011529 | 0.006769 | 0.041779 | 0.007706 | 0.011367 | 0.010920 | 0.037969 | 0.008070 | 0.011415 | 0.008711 | 0.039800 |
Cell Periphery | 0.002928 | 0.000728 | 0.001913 | 0.025748 | 0.014687 | 0.000909 | 0.004964 | 0.024145 | 0.010664 | 0.000855 | 0.003340 | 0.024916 |
Cytoplasm | 0.085696 | 0.276417* | 0.059052 | 0.050841 | 0.179514* | 0.244459* | 0.028445 | 0.058174 | 0.147418* | 0.254055* | 0.044736 | 0.054649 |
Cytoplasmic Foci | 0.037793 | 0.092424 | 0.011740 | 0.012482 | 0.039199 | 0.100465 | 0.002956 | 0.009067 | 0.038718 | 0.098050 | 0.007631 | 0.010708 |
Eisosomes | 0.000359 | 0.000222 | 0.000094 | 0.000474 | 0.000832 | 0.000405 | 0.000200 | 0.000301 | 0.000670 | 0.000350 | 0.000144 | 0.000384 |
Endoplasmic Reticulum | 0.553959* | 0.369170* | 0.761305* | 0.685248* | 0.563847* | 0.351298* | 0.868735* | 0.756054* | 0.560464* | 0.356664* | 0.811557* | 0.722020* |
Endosome | 0.025361 | 0.017663 | 0.010925 | 0.016593 | 0.019590 | 0.027687 | 0.007025 | 0.011805 | 0.021564 | 0.024677 | 0.009101 | 0.014106 |
Golgi | 0.024876 | 0.002927 | 0.000601 | 0.009733 | 0.007849 | 0.006443 | 0.001322 | 0.006090 | 0.013674 | 0.005387 | 0.000938 | 0.007841 |
Lipid Particles | 0.015438 | 0.028426 | 0.007447 | 0.043751 | 0.016558 | 0.034933 | 0.007723 | 0.029088 | 0.016175 | 0.032979 | 0.007576 | 0.036136 |
Mitochondria | 0.009168 | 0.002724 | 0.000404 | 0.004740 | 0.002181 | 0.006226 | 0.000493 | 0.001690 | 0.004571 | 0.005174 | 0.000446 | 0.003156 |
Mitotic Spindle | 0.012060 | 0.000245 | 0.000552 | 0.004082 | 0.001727 | 0.000857 | 0.000741 | 0.001624 | 0.005262 | 0.000673 | 0.000640 | 0.002805 |
None | 0.000060 | 0.001755 | 0.000368 | 0.000495 | 0.000234 | 0.003169 | 0.000126 | 0.000915 | 0.000174 | 0.002745 | 0.000255 | 0.000713 |
Nuclear Periphery | 0.002582 | 0.008297 | 0.016683 | 0.001643 | 0.000399 | 0.007766 | 0.001382 | 0.001250 | 0.001146 | 0.007925 | 0.009526 | 0.001439 |
Nuclear Periphery Foci | 0.015329 | 0.024205 | 0.045341 | 0.016028 | 0.001728 | 0.007430 | 0.016855 | 0.008602 | 0.006381 | 0.012467 | 0.032016 | 0.012171 |
Nucleolus | 0.000098 | 0.000803 | 0.000158 | 0.000058 | 0.000299 | 0.000706 | 0.000017 | 0.000050 | 0.000230 | 0.000735 | 0.000092 | 0.000054 |
Nucleus | 0.007735 | 0.069433 | 0.041406 | 0.007108 | 0.007037 | 0.045722 | 0.010288 | 0.007328 | 0.007276 | 0.052842 | 0.026850 | 0.007222 |
Peroxisomes | 0.000529 | 0.002427 | 0.000236 | 0.000408 | 0.003317 | 0.004905 | 0.000384 | 0.000347 | 0.002363 | 0.004161 | 0.000305 | 0.000376 |
Vacuole | 0.190415 | 0.082971 | 0.030595 | 0.064471 | 0.116467 | 0.126626 | 0.029186 | 0.035150 | 0.141765 | 0.113518 | 0.029936 | 0.049244 |
Vacuole Periphery | 0.003175 | 0.001571 | 0.000293 | 0.003422 | 0.006986 | 0.003980 | 0.000244 | 0.002710 | 0.005682 | 0.003257 | 0.000270 | 0.003052 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.75 | 0.20 | -3.03 | -4.59 | -3.40 | -0.85 | -2.21 | -4.38 | -3.76 | 0.97 | 0.16 | -2.10 | -5.69 | -6.23 | -0.62 |
Bud Neck | -2.99 | -2.07 | -7.13 | -1.94 | -5.24 | -1.23 | 1.60 | 1.28 | 4.62 | -1.03 | -1.79 | 1.51 | 0.22 | 3.60 | -4.01 |
Bud Site | -0.75 | 0.37 | -7.47 | -6.90 | -8.54 | -1.43 | -1.49 | -7.89 | -7.03 | -5.01 | -1.58 | -0.93 | -11.12 | -10.08 | -9.51 |
Cell Periphery | 2.68 | 1.00 | -9.79 | -11.46 | -10.65 | 3.53 | 2.42 | -2.01 | -10.29 | -7.95 | 3.75 | 2.74 | -4.42 | -15.25 | -13.07 |
Cytoplasm | -7.16 | 1.33 | 1.08 | 13.08 | -0.73 | -3.34 | 7.46 | 6.32 | 17.52 | -2.99 | -6.65 | 6.68 | 5.82 | 22.02 | -2.49 |
Cytoplasmic Foci | -4.96 | 3.26 | 3.26 | 10.06 | -0.23 | -7.35 | 5.35 | 4.29 | 16.69 | -4.28 | -8.93 | 5.84 | 5.27 | 19.32 | -1.97 |
Eisosomes | 1.00 | 1.95 | -0.87 | -4.06 | -6.20 | 1.60 | 2.33 | 1.99 | 1.29 | -1.31 | 1.75 | 2.88 | 1.55 | -0.68 | -5.10 |
Endoplasmic Reticulum | 2.86 | -4.17 | -2.10 | -10.72 | 5.26 | 6.41 | -7.55 | -5.19 | -21.48 | 4.70 | 6.76 | -7.99 | -4.82 | -22.82 | 6.67 |
Endosome | 1.22 | 2.47 | 1.53 | 0.58 | -3.25 | -2.33 | 3.82 | 2.38 | 8.11 | -3.43 | -1.14 | 4.27 | 2.58 | 6.87 | -4.56 |
Golgi | 2.04 | 2.27 | 1.41 | -3.74 | -5.32 | 0.85 | 4.18 | 1.05 | 0.29 | -3.70 | 2.15 | 3.42 | 1.55 | -1.80 | -6.54 |
Lipid Particles | -2.77 | 2.00 | -5.59 | -3.12 | -9.50 | -4.91 | 2.83 | -3.40 | 1.56 | -7.29 | -5.69 | 3.55 | -6.49 | -1.02 | -11.99 |
Mitochondria | 1.24 | 1.75 | 0.83 | -1.08 | -2.93 | -3.11 | 1.77 | 0.40 | 3.51 | -1.24 | -0.42 | 2.27 | 0.70 | 1.94 | -3.13 |
Mitotic Spindle | 1.10 | 1.06 | 0.53 | -4.48 | -4.15 | 0.54 | 0.45 | -0.23 | -1.98 | -1.49 | 1.20 | 1.17 | 0.28 | -4.63 | -4.36 |
None | -3.62 | -3.64 | -3.44 | 2.55 | -0.90 | -2.79 | 0.57 | -2.11 | 2.06 | -2.46 | -3.43 | -1.17 | -2.96 | 2.63 | -2.53 |
Nuclear Periphery | -2.13 | -4.07 | 0.51 | 3.13 | 4.85 | -5.18 | -5.35 | -3.63 | 4.48 | 0.62 | -5.20 | -4.84 | -0.60 | 5.36 | 4.84 |
Nuclear Periphery Foci | -1.93 | -5.40 | -0.50 | 1.90 | 5.74 | -4.84 | -5.07 | -7.60 | -1.36 | 2.46 | -3.48 | -8.22 | -4.41 | -0.44 | 6.29 |
Nucleolus | -3.12 | -0.98 | 0.83 | 3.38 | 2.10 | -2.17 | 1.80 | 1.57 | 5.65 | -3.28 | -3.49 | 1.22 | 1.68 | 6.51 | 1.50 |
Nucleus | -8.29 | -4.73 | -0.38 | 8.87 | 4.94 | -10.39 | -2.53 | -0.82 | 10.67 | 2.17 | -13.17 | -5.55 | -1.06 | 13.88 | 5.37 |
Peroxisomes | -4.13 | 2.04 | 0.97 | 4.56 | -1.55 | -1.79 | 4.20 | 4.45 | 7.50 | 0.33 | -2.88 | 4.45 | 4.43 | 8.49 | -0.42 |
Vacuole | 3.70 | 5.16 | 3.57 | -0.62 | -7.79 | -0.65 | 4.07 | 4.43 | 11.09 | 0.29 | 2.12 | 6.62 | 5.09 | 7.78 | -5.16 |
Vacuole Periphery | 1.09 | 2.09 | -0.16 | -2.52 | -4.83 | 1.80 | 4.13 | 2.50 | 1.72 | -4.14 | 1.98 | 4.59 | 2.18 | 0.56 | -6.33 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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