Standard name
Human Ortholog
Description Ribosomal protein P1 beta; component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; free (non-ribosomal) P1 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; accumulation is regulated by phosphorylation and interaction with the P2 stalk component

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0.05 0.06 0.06 0.06 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 1.0 1.0 1.0 0.99 0.98 0.95 0.89 0.89 0.79 0.83 0.83 1.0 0.99 0.99 0.99 0.99 0.97 0.93 0.94 0.93 0.92 0.88 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0.08 0.08 0.15 0.11 0.12 0 0 0 0.08 0.07 0.12 0 0 0 0.05 0.06 0.06
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 2 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 7 1 0 0 1 1 0 0 0 0 0 0 1 0 0 0 4 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 4 2 16 10 15 14 12 1 1 2 11 6 13 1 0 0 0 0 0
Cytoplasm 214 84 139 164 291 179 335 193 202 181 178 211 390 448 554 296 244 187 79 124 177 324 338
Endoplasmic Reticulum 1 0 0 1 4 7 31 17 37 23 26 1 0 3 45 21 31 4 1 3 9 21 23
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 1 1 1 5 3 2 0 0 1 1 0 1 0 0 0 0 2 0
Mitochondria 0 0 0 0 0 0 0 1 1 1 2 0 0 0 5 1 8 0 0 0 0 0 0
Nucleus 0 0 0 0 1 0 2 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 1 0 0 0 2 1 1 0 1 0 2 0 0 0 0 0 0 2 3
Unique Cell Count 215 84 139 165 297 189 376 216 255 217 214 212 392 453 561 299 252 201 84 134 193 368 380
Labelled Cell Count 215 84 140 165 301 196 386 223 264 226 226 213 392 454 618 325 297 201 84 134 193 368 380


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 288.6 282.4 234.0 232.1 191.4 108.5 127.2 100.2 99.4 87.5 85.6 294.9 306.5 300.6 358.2 383.2 402.7 287.6 302.1 326.0
Std Deviation (1e-4) 46.8 48.3 50.5 40.8 37.0 33.0 30.5 25.4 30.6 25.7 31.6 63.4 58.8 62.9 84.1 77.5 84.5 42.5 67.5 68.8
Intensity Change (Log2) -0.01 -0.29 -1.11 -0.88 -1.22 -1.24 -1.42 -1.45 0.33 0.39 0.36 0.61 0.71 0.78 0.3 0.37 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 2.9 3.1 0 0 0 0 0 0 0
Cytoplasm -0.9 -1.7 -2.8 -4.1 -4.0 -5.8 -5.1 -5.1 -0.8 -0.8 -1.2 -1.3 -1.2 -2.1
Endoplasmic Reticulum 0 0 0 3.5 3.4 4.7 4.0 4.3 0 0 0 3.5 3.2 4.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 218.8054 287.1865 253.3063 256.1999 195.3645 205.9183 263.5332 329.0664 297.4363 268.3692 276.5456 201.9583 281.5579 346.0036 310.8163 318.8788 292.4081 302.0321
Actin 0.0102 0.0006 0.0026 0.0005 0.0022 0.0489 0.0042 0.0006 0.0013 0.0004 0.0015 0.0012 0.0025 0.0007 0.0008 0.0005 0.0012 0.0035
Bud 0.0029 0.0022 0.0054 0.0028 0.004 0.0016 0.0027 0.002 0.0029 0.0027 0.003 0.0071 0.0027 0.0013 0.0027 0.0024 0.0025 0.005
Bud Neck 0.001 0.0012 0.0016 0.0015 0.0016 0.0043 0.0009 0.0008 0.0012 0.0021 0.0035 0.0011 0.0011 0.0007 0.0015 0.0017 0.0019 0.0042
Bud Periphery 0.0044 0.0017 0.0078 0.0022 0.0024 0.0024 0.0045 0.0017 0.0024 0.0021 0.0022 0.0065 0.0044 0.0006 0.0018 0.0014 0.0013 0.0023
Bud Site 0.0016 0.0014 0.0033 0.0008 0.0016 0.0006 0.0021 0.0011 0.0017 0.001 0.0004 0.0007 0.0021 0.0009 0.0012 0.0005 0.0013 0.0004
Cell Periphery 0.0852 0.0477 0.0687 0.0771 0.0169 0.0384 0.0937 0.0431 0.0632 0.0761 0.0149 0.0108 0.0829 0.0274 0.0399 0.0241 0.0168 0.0145
Cytoplasm 0.4178 0.7512 0.4667 0.3827 0.5274 0.4266 0.4733 0.7995 0.6229 0.3334 0.6204 0.4559 0.5087 0.8531 0.6841 0.5926 0.6754 0.564
Cytoplasmic Foci 0.0141 0.0008 0.0076 0.0008 0.0118 0.0082 0.0128 0.0009 0.0097 0.0405 0.0045 0.06 0.0111 0.0007 0.0091 0.0032 0.0129 0.0099
Eisosomes 0.0012 0.0001 0.0005 0.0001 0.0004 0.001 0.0025 0.0001 0.0005 0.0002 0.0003 0.0004 0.002 0.0002 0.0002 0.0003 0.0006 0.0001
Endoplasmic Reticulum 0.0256 0.0156 0.0047 0.0021 0.0036 0.0417 0.0328 0.0076 0.0206 0.0057 0.0095 0.0058 0.0397 0.0045 0.006 0.0068 0.0052 0.0134
Endosome 0.0119 0.0021 0.0138 0.0043 0.0237 0.0322 0.0144 0.002 0.0124 0.0122 0.0388 0.0094 0.0046 0.0011 0.0026 0.0042 0.0156 0.0735
Golgi 0.0025 0.0004 0.0083 0.0002 0.0081 0.0445 0.0076 0.0004 0.0025 0.0005 0.0053 0.0006 0.0009 0.0002 0.0004 0.0003 0.003 0.0035
Lipid Particles 0.0068 0.001 0.0052 0.0025 0.0027 0.0129 0.0068 0.0017 0.0041 0.0066 0.0042 0.0029 0.0059 0.0009 0.0053 0.0021 0.0136 0.0051
Mitochondria 0.0258 0.0027 0.0215 0.0013 0.0379 0.0667 0.0219 0.0011 0.0165 0.002 0.0403 0.0148 0.0181 0.0077 0.0112 0.0084 0.0206 0.0141
None 0.0064 0.0006 0.0043 0.0004 0.0283 0.0012 0.009 0.0004 0.0046 0.0251 0.0059 0.0759 0.009 0.001 0.0067 0.0036 0.0055 0.0034
Nuclear Periphery 0.0536 0.0111 0.013 0.0441 0.0096 0.0542 0.0384 0.0068 0.0146 0.0099 0.0157 0.0104 0.0645 0.0082 0.0176 0.0268 0.0184 0.0571
Nucleolus 0.0184 0.0015 0.0152 0.0065 0.0138 0.001 0.0101 0.0007 0.0084 0.0109 0.0049 0.0214 0.0125 0.0026 0.0145 0.0128 0.0075 0.0126
Nucleus 0.0228 0.0067 0.0142 0.0171 0.0166 0.0085 0.0159 0.0048 0.0098 0.0131 0.0087 0.0199 0.0245 0.0101 0.0221 0.0293 0.0164 0.0258
Peroxisomes 0.0017 0.0001 0.005 0.0002 0.0008 0.0062 0.0012 0.0001 0.0007 0.0005 0.0003 0.0005 0.0007 0.0001 0.0011 0.0004 0.0037 0.0009
Punctate Nuclear 0.0028 0.0001 0.0002 0.0001 0.0004 0.0006 0.0005 0 0.0006 0.014 0.0003 0.0116 0.0009 0 0.0004 0.0001 0.0004 0.001
Vacuole 0.2126 0.1286 0.2547 0.3342 0.187 0.1055 0.1952 0.1095 0.1644 0.3719 0.1659 0.1849 0.157 0.0684 0.1346 0.1994 0.1224 0.0832
Vacuole Periphery 0.0704 0.0226 0.0758 0.1185 0.0993 0.0928 0.0494 0.0151 0.0348 0.0691 0.0495 0.098 0.0443 0.0093 0.0361 0.0791 0.0539 0.1027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2964.7549 3665.8778 4808.8727 2563.3586 3529.7345 4352.771 2721.669 2389.494 3436.4959 2294.0503
Translational Efficiency 1.2096 1.13 0.9505 2.7461 1.4914 0.8148 1.384 2.1078 1.2024 1.6194

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1036 236 2309 1790 1166 2463 2072 1709 2202 2699 4381 3499

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 8938.09 14832.55 24705.22 22242.63 13618.33 15251.77 23247.00 20899.68 11416.36 15215.11 24015.55 21586.70
Standard Deviation 2105.13 3040.12 4612.48 5614.59 2996.51 3434.37 4814.88 5315.05 3506.66 3403.78 4765.24 5511.37
Intensity Change Log 2 0.730728 1.466777 1.315289 0.163427 0.771495 0.617931 0.415474 1.088059 0.935565

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000143 0.000094 0.000068 0.000285 0.000066 0.000058 0.000102 0.000144 0.000102 0.000061 0.000084 0.000216
Bud Neck 0.000671 0.000900 0.000493 0.000849 0.001008 0.000286 0.000431 0.001245 0.000849 0.000340 0.000463 0.001043
Bud Site 0.005259 0.005297 0.001447 0.005893 0.001651 0.002845 0.002014 0.005316 0.003348 0.003060 0.001715 0.005611
Cell Periphery 0.028576 0.090496 0.053589 0.081768 0.022304 0.015103 0.071352 0.037376 0.025255 0.021695 0.061990 0.060086
Cytoplasm 0.243760* 0.243018* 0.426813* 0.301693* 0.324473* 0.535940* 0.431699* 0.329138* 0.286499* 0.510327* 0.429124* 0.315098*
Cytoplasmic Foci 0.024459 0.011044 0.004999 0.004533 0.014103 0.013812 0.003316 0.003699 0.018975 0.013570 0.004203 0.004126
Eisosomes 0.000003 0.000005 0.000003 0.000006 0.000003 0.000001 0.000004 0.000004 0.000003 0.000002 0.000003 0.000005
Endoplasmic Reticulum 0.002553 0.003223 0.003699 0.002640 0.000821 0.002458 0.002145 0.001302 0.001636 0.002525 0.002964 0.001987
Endosome 0.001480 0.001360 0.000543 0.000824 0.000863 0.001037 0.000454 0.001036 0.001153 0.001065 0.000501 0.000928
Golgi 0.197624* 0.112999* 0.036422 0.024620 0.110442* 0.062966 0.024965 0.019920 0.151460* 0.067341 0.031004 0.022324
Lipid Particles 0.023804 0.057757 0.021942 0.032908 0.027077 0.014204 0.029180 0.021293 0.025537 0.018012 0.025365 0.027235
Mitochondria 0.000745 0.000207 0.000393 0.000996 0.000161 0.000602 0.000367 0.000411 0.000436 0.000568 0.000381 0.000710
Mitotic Spindle 0.002114 0.001267 0.000517 0.000596 0.001211 0.001204 0.000330 0.000696 0.001636 0.001209 0.000429 0.000645
None 0.002674 0.001166 0.001043 0.003061 0.002422 0.002113 0.001090 0.001014 0.002541 0.002031 0.001065 0.002061
Nuclear Periphery 0.001403 0.001644 0.001536 0.000990 0.002634 0.000637 0.001023 0.000656 0.002055 0.000726 0.001293 0.000827
Nuclear Periphery Foci 0.014751 0.016313 0.008630 0.006890 0.024393 0.009641 0.007299 0.005975 0.019857 0.010224 0.008000 0.006443
Nucleolus 0.000119 0.000098 0.000125 0.000236 0.000109 0.000101 0.000135 0.000299 0.000114 0.000101 0.000130 0.000267
Nucleus 0.009205 0.003030 0.004169 0.006718 0.008123 0.003924 0.004072 0.009353 0.008632 0.003846 0.004123 0.008005
Peroxisomes 0.000018 0.000084 0.000137 0.000217 0.000009 0.000042 0.000193 0.000099 0.000013 0.000045 0.000164 0.000159
Vacuole 0.159329 0.286022* 0.274818* 0.411562* 0.250230* 0.165456 0.322971* 0.465226* 0.207463* 0.175998 0.297592* 0.437773*
Vacuole Periphery 0.281309* 0.163976* 0.158614* 0.112714* 0.207897* 0.167569* 0.096859 0.095800 0.242436* 0.167255* 0.129407* 0.104453*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.85 2.95 -2.88 -4.31 -5.00 0.71 -1.57 -2.64 -3.39 -1.60 2.30 1.26 -3.90 -5.63 -5.17
Bud Neck -1.15 0.68 -3.33 -1.18 -4.11 6.43 5.36 -3.76 -6.90 -6.41 6.10 4.28 -4.75 -8.62 -7.56
Bud Site 0.11 3.96 -0.92 -1.05 -7.13 -3.48 -1.55 -7.85 -4.57 -6.71 0.34 3.30 -4.10 -5.38 -9.71
Cell Periphery -8.69 -10.41 -14.97 1.76 -7.18 5.07 -19.99 -4.84 -9.32 13.97 2.71 -21.53 -15.13 -16.89 2.69
Cytoplasm 0.09 -14.83 -4.85 -2.95 11.15 -17.25 -8.30 -1.06 18.57 8.23 -22.90 -15.77 -3.76 21.62 13.67
Cytoplasmic Foci 5.89 11.28 11.19 3.73 0.39 1.00 9.52 8.92 12.37 -1.71 5.04 14.60 14.19 12.50 -0.71
Eisosomes -2.03 1.11 -3.91 -1.69 -5.22 6.55 -1.74 -1.71 -6.52 -0.34 6.26 -0.12 -4.34 -9.16 -4.62
Endoplasmic Reticulum -0.88 -2.55 0.11 0.97 3.02 -6.06 -7.03 -2.08 4.29 4.30 -3.03 -5.59 -1.04 2.32 4.81
Endosome 0.84 10.29 5.96 3.60 -4.01 -2.58 7.95 -1.42 0.09 -5.32 1.20 12.78 2.89 1.88 -6.63
Golgi 8.36 28.35 30.69* 10.58 8.60 12.63 24.95 27.17 22.98 5.57 22.46* 35.73 38.91 24.23 10.29
Lipid Particles -5.35 1.10 -3.58 4.14 -5.66 6.67 -0.97 3.42 -4.11 5.61 5.05 0.17 -0.20 -5.95 -0.46
Mitochondria 3.10 2.11 -1.10 -4.63 -3.61 -4.48 -3.58 -3.16 1.46 -0.59 -0.80 0.90 -2.21 -1.52 -3.53
Mitotic Spindle 2.18 4.66 4.46 2.57 -0.74 -0.51 9.42 1.71 1.81 -2.05 1.43 7.17 4.69 3.07 -2.15
None 3.10 3.38 -0.34 -2.39 -2.57 0.28 1.44 1.54 2.97 0.17 0.86 2.82 0.73 -0.06 -2.36
Nuclear Periphery -1.07 -1.86 3.74 3.16 5.89 10.04 8.03 9.71 -0.80 5.59 11.01 5.35 10.21 -2.30 7.76
Nuclear Periphery Foci -0.96 6.28 8.19 6.96 3.86 11.77 14.11 14.96 6.32 2.40 11.17 14.95 16.77 7.53 4.54
Nucleolus 2.09 -0.34 -7.50 -9.97 -8.82 0.23 -2.55 -8.16 -8.07 -7.55 0.86 -1.79 -11.01 -10.97 -10.87
Nucleus 3.38 2.76 0.40 -4.72 -4.02 4.65 4.72 -3.68 -8.16 -8.22 4.84 4.64 -1.74 -8.79 -8.64
Peroxisomes -4.00 -7.62 -5.63 -3.26 -1.93 -8.95 -6.17 -7.77 -4.30 3.21 -8.70 -9.18 -7.66 -5.68 0.52
Vacuole -7.46 -15.60 -29.31 -7.50 -16.20 10.67 -7.70 -20.83 -35.46 -15.07 5.21 -14.95 -33.77 -41.17 -22.12
Vacuole Periphery 10.24 15.73 22.36* 5.95 11.17 5.69 17.09 18.71 17.96 3.00 13.10 21.96 28.49 17.42 10.75
Standard name
Human Ortholog
Description Ribosomal protein P1 beta; component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; free (non-ribosomal) P1 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; accumulation is regulated by phosphorylation and interaction with the P2 stalk component
Localization
Cell Percentages cytoplasm (95%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rpp1b

Rpp1b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpp1b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available