Standard name
Human Ortholog
Description Cullin; structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation; human homolog CUL1 can complement yeast cdc53 null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.06
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.12 0.15 0.19 0.46 0.48 0.54 – 0.56 0.61 0.56 0.5 0.21 0.27 0.32 0.32 0.31 0.35 0.07 0.07 0.1 0.06 0.1 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.13 0 0.19 0.26 – 0.4 0.4 0.5 0.47 0.09 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.53 0.51 0.78 0.49 0.39 0.17 0.16 – 0.13 0.11 0.11 0.1 0.37 0.28 0.21 0.27 0.22 0.19 0.68 0.68 0.66 0.51 0.32 0.32
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.5 0.52 0.16 0.32 0.2 0.24 0.14 – 0.12 0.08 0 0.06 0.44 0.53 0.52 0.53 0.58 0.58 0.12 0.14 0.14 0.27 0.35 0.3
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 2
Bud 0 0 0 2 0 0 4 – 3 0 3 1 0 0 1 0 0 0 6 15 3 24 18 27
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 1 4 9
Bud Site 0 0 0 0 0 0 2 – 2 2 3 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 1 0 0 – 0 1 0 1 0 0 1 0 1 1 0 0 0 0 0 0
Cytoplasm 29 46 25 15 61 69 101 – 75 75 74 56 42 73 84 100 83 64 16 31 20 18 35 38
Endoplasmic Reticulum 0 0 0 0 1 1 1 – 0 0 0 0 1 1 0 17 11 11 0 1 1 1 8 13
Endosome 3 2 0 0 0 4 1 – 1 0 0 0 0 2 13 6 4 9 6 4 2 3 9 15
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 2 2 2 2 0 0 3 2
Mitochondria 3 5 4 10 4 28 48 – 54 49 67 53 18 4 5 4 1 1 2 2 5 1 10 13
Nucleus 115 192 131 39 52 24 31 – 18 13 14 11 73 78 54 86 57 35 165 290 137 142 116 141
Nuclear Periphery 0 0 0 0 0 0 0 – 1 0 0 1 0 0 0 0 0 0 4 1 0 0 4 4
Nucleolus 0 0 0 0 0 0 0 – 0 0 1 1 1 0 1 0 0 0 3 4 1 3 4 8
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 3 8 4 4 8 17
Vac/Vac Membrane 109 194 27 25 26 35 26 – 16 10 3 7 87 145 134 167 154 105 28 61 28 74 127 134
Unique Cell Count 219 375 169 79 132 145 188 135 123 133 113 198 275 260 317 265 182 244 428 210 279 365 447
Labelled Cell Count 259 439 188 91 145 161 214 170 150 165 133 222 303 293 382 313 228 244 428 210 279 365 447


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 7.9 6.6 6.2 6.8 6.0 5.3 – 4.7 4.5 4.0 4.0 6.5 7.6 7.8 10.6 10.8 10.1 6.1 6.4 7.0
Std Deviation (1e-4) 1.1 1.4 0.9 1.2 1.6 1.9 1.3 – 1.3 1.5 1.5 1.0 1.1 1.4 1.6 2.4 2.2 2.1 1.2 1.5 1.6
Intensity Change (Log2) – – – -0.09 0.06 -0.14 -0.3 – -0.49 -0.55 -0.71 -0.73 -0.01 0.21 0.25 0.7 0.72 0.62 -0.1 -0.05 0.09

WT3RAP60RAP140RAP220RAP300RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 6.0 6.3 7.7 – 7.5 8.2 7.5 6.3 1.6 2.9 4.1 4.0 3.9 4.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 3.1 0 3.2
Endosome 0 0 0 0 – 0 0 0 0 0 0 3.0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.9 6.2 – 0 0 0 0 2.7 0 0 0 0 0
Nucleus -4.4 -6.7 -10.8 -11.6 – -11.1 -11.3 -11.6 -11.2 -7.8 -10.1 -11.6 -10.6 -11.5 -10.9
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 2.8 0.8 1.8 -0.6 – -1.0 -2.0 -4.0 -2.5 5.8 7.7 7.4 7.9 8.7 8.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.3815 5.1832 4.6097 4.0205 4.4241 4.9496 3.1037 4.0521 3.2731 2.8378 3.0084 3.3988 3.131 3.6324 3.0818 2.9081 3.2741 3.1912
Actin 0.0766 0.0002 0.0107 0.0005 0.004 0.0041 0.0384 0.0001 0.0081 0.0304 0.0008 0.0037 0.0177 0.0001 0.01 0.0002 0.0469 0.0073
Bud 0.0008 0.0001 0.0007 0.0005 0.0002 0.0003 0.001 0.0001 0.0003 0.0003 0.0003 0.0002 0.0004 0.0001 0.0002 0.0001 0.0005 0.0001
Bud Neck 0.0068 0.0006 0.0025 0.0008 0.0029 0.0048 0.005 0.0002 0.0008 0.0016 0.0026 0.0041 0.0072 0.0004 0.0006 0.001 0.0017 0.0021
Bud Periphery 0.0011 0.0001 0.0008 0.0011 0.0003 0.0003 0.0017 0 0.0002 0.0004 0.0012 0.0003 0.0005 0 0.0004 0 0.0009 0.0002
Bud Site 0.0124 0.0009 0.0039 0.0011 0.0006 0.0016 0.0056 0.0003 0.0016 0.0009 0.0004 0.0003 0.003 0.0003 0.0003 0.0003 0.0013 0.0003
Cell Periphery 0.0005 0.0001 0.0004 0.0002 0.0001 0.0001 0.0003 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0
Cytoplasm 0.0716 0.089 0.0803 0.0629 0.0388 0.0542 0.0405 0.0771 0.0567 0.0762 0.0959 0.0347 0.0322 0.0342 0.0329 0.0899 0.1174 0.0374
Cytoplasmic Foci 0.0261 0.0005 0.0057 0.0024 0.0213 0.0072 0.0189 0.0002 0.007 0.0071 0.0006 0.0025 0.0154 0.0002 0.0004 0.0289 0.0061 0.0022
Eisosomes 0.0006 0 0.0002 0 0.0001 0.0001 0.0003 0 0 0.0001 0 0 0.0001 0 0.0006 0 0.001 0.0001
Endoplasmic Reticulum 0.0048 0.0039 0.0063 0.0016 0.0045 0.0051 0.016 0.0009 0.0067 0.0041 0.0026 0.0019 0.0064 0.001 0.0012 0.0013 0.0048 0.0008
Endosome 0.0243 0.0017 0.0089 0.0292 0.0397 0.0132 0.0436 0.0005 0.0218 0.0126 0.0055 0.0052 0.02 0.0006 0.0018 0.0069 0.0158 0.0024
Golgi 0.0086 0 0.0021 0.0045 0.008 0.0049 0.0094 0 0.0079 0.0064 0.0002 0.0027 0.0035 0 0.0008 0.0008 0.0063 0.0009
Lipid Particles 0.0139 0.0001 0.0019 0.0023 0.0613 0.0042 0.0096 0 0.0025 0.001 0.0003 0.0034 0.0041 0 0.0012 0.0034 0.0045 0.0006
Mitochondria 0.0142 0.0003 0.0094 0.0257 0.0041 0.0144 0.0078 0.0002 0.0032 0.0022 0.0015 0.0013 0.0012 0.0002 0.0107 0.0003 0.0028 0.0039
None 0.0444 0.014 0.0326 0.0621 0.0316 0.0169 0.0169 0.0004 0.0015 0.0081 0.0011 0.0004 0.0087 0.0012 0.0123 0.0028 0.0037 0.0013
Nuclear Periphery 0.022 0.0232 0.0165 0.0089 0.0282 0.0118 0.0496 0.0183 0.0262 0.0296 0.0474 0.017 0.0214 0.0095 0.0114 0.0143 0.035 0.0078
Nucleolus 0.0058 0.0024 0.0034 0.0024 0.006 0.0072 0.0027 0.0019 0.0019 0.0024 0.0062 0.0028 0.0026 0.0012 0.002 0.0017 0.0025 0.0022
Nucleus 0.61 0.853 0.7878 0.7757 0.6946 0.828 0.7012 0.8902 0.8416 0.8016 0.8218 0.9067 0.8349 0.945 0.9085 0.7952 0.731 0.9188
Peroxisomes 0.0265 0 0.0035 0.0005 0.0134 0.002 0.0085 0 0.0008 0.0005 0.0003 0.0031 0.0043 0 0.0008 0.0048 0.003 0.0012
Punctate Nuclear 0.0227 0.0046 0.0121 0.0073 0.0328 0.0166 0.0106 0.0083 0.0074 0.0106 0.0067 0.0078 0.0135 0.0052 0.0024 0.047 0.0117 0.0095
Vacuole 0.0053 0.0048 0.0085 0.0078 0.0064 0.0026 0.008 0.001 0.0032 0.0031 0.0035 0.0014 0.0024 0.0006 0.001 0.001 0.0018 0.0007
Vacuole Periphery 0.001 0.0004 0.0019 0.0026 0.0012 0.0005 0.0043 0.0002 0.0006 0.0007 0.001 0.0004 0.0006 0.0001 0.0006 0.0002 0.0009 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.7351 37.4726 38.271 54.6072 38.5704 39.1924 42.7774 51.5569 48.0832 46.8725
Translational Efficiency 0.9422 0.9982 1.0553 0.6714 0.7977 1.0044 0.988 0.7207 0.8591 0.8456

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
233 920 1225 1289 2320 1562 1982 71 2553 2482 3207 1360

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 668.22 842.57 1240.37 1422.27 755.95 790.85 1272.37 1469.49 747.94 810.02 1260.15 1424.74
Standard Deviation 69.51 123.09 170.89 203.35 118.27 112.42 180.65 231.80 117.44 119.14 177.67 205.20
Intensity Change Log 2 — 0.334473 0.892375 1.089800 — 0.065113 0.751156 0.958953 — 0.197774 0.819140 1.021826

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000082 0.000310 0.000562 0.001006 0.000075 0.000276 0.000306 0.000799 0.000075 0.000288 0.000404 0.000995
Bud Neck 0.000895 0.002475 0.013118 0.017363 0.002029 0.004887 0.014734 0.007868 0.001925 0.003993 0.014117 0.016868
Bud Site 0.001670 0.001455 0.001396 0.008625 0.000878 0.003396 0.001722 0.002299 0.000950 0.002677 0.001597 0.008295
Cell Periphery 0.000127 0.000147 0.000114 0.000189 0.000102 0.000159 0.000102 0.000178 0.000104 0.000155 0.000106 0.000189
Cytoplasm 0.299974 0.173156 0.012954 0.061460 0.208654 0.203424 0.041846 0.052338 0.216988 0.192205 0.030810 0.060984
Cytoplasmic Foci 0.045136 0.011486 0.000137 0.003765 0.011841 0.017969 0.000070 0.000290 0.014879 0.015566 0.000095 0.003583
Eisosomes 0.000045 0.000032 0.000016 0.000020 0.000015 0.000033 0.000014 0.000021 0.000018 0.000033 0.000015 0.000020
Endoplasmic Reticulum 0.001125 0.003824 0.003038 0.003448 0.001205 0.003033 0.003365 0.004369 0.001198 0.003326 0.003240 0.003496
Endosome 0.000765 0.001262 0.000251 0.006817 0.000515 0.002070 0.000241 0.000439 0.000538 0.001770 0.000245 0.006484
Golgi 0.000093 0.000223 0.000056 0.007693 0.000158 0.001186 0.000035 0.000087 0.000152 0.000829 0.000043 0.007296
Lipid Particles 0.002319 0.001242 0.000130 0.000980 0.000753 0.002207 0.000084 0.000352 0.000896 0.001850 0.000102 0.000947
Mitochondria 0.000363 0.002892 0.001714 0.003268 0.001081 0.004124 0.001681 0.001450 0.001016 0.003667 0.001694 0.003173
Mitotic Spindle 0.000453 0.004343 0.002060 0.101344 0.000600 0.007628 0.008330 0.107927 0.000587 0.006410 0.005935 0.101687
None 0.006177 0.008314 0.002540 0.006330 0.012288 0.009243 0.004024 0.006537 0.011730 0.008899 0.003457 0.006341
Nuclear Periphery 0.000695 0.000974 0.000890 0.002312 0.000978 0.001501 0.001765 0.001399 0.000952 0.001306 0.001431 0.002264
Nuclear Periphery Foci 0.000394 0.001136 0.000161 0.004362 0.000425 0.001122 0.000124 0.000226 0.000422 0.001127 0.000138 0.004146
Nucleolus 0.001001 0.001928 0.000740 0.000876 0.001813 0.002895 0.000513 0.000339 0.001739 0.002536 0.000600 0.000848
Nucleus 0.631446 0.773509 0.956970 0.744965 0.752485 0.720970 0.916390 0.801029 0.741438 0.740444 0.931891 0.747891
Peroxisomes 0.000528 0.000991 0.000067 0.000563 0.000406 0.002833 0.000024 0.000066 0.000417 0.002150 0.000040 0.000537
Vacuole 0.006612 0.009981 0.003007 0.019236 0.003586 0.010732 0.004424 0.011904 0.003862 0.010453 0.003883 0.018853
Vacuole Periphery 0.000099 0.000322 0.000080 0.005379 0.000115 0.000314 0.000206 0.000082 0.000113 0.000317 0.000158 0.005102

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.06 -12.89 -12.87 -9.27 -5.80 -6.71 -16.07 -7.95 -6.73 -5.86 -7.78 -21.24 -14.65 -11.36 -8.57
Bud Neck -4.56 -12.39 -17.92 -16.23 -4.65 -4.06 -14.58 -4.52 -3.97 -1.78 -4.45 -18.50 -17.72 -15.26 -5.27
Bud Site 0.27 0.01 -3.69 -7.61 -7.29 -4.14 -5.59 -4.24 -2.91 -3.53 -4.11 -5.12 -8.89 -6.22 -7.46
Cell Periphery -1.16 0.66 -5.99 -4.97 -8.55 -4.02 -0.45 -4.94 -3.98 -4.90 -4.73 -0.98 -11.47 -6.03 -12.06
Cytoplasm 6.93 16.92 14.86 16.35 -13.85 1.00 33.50 13.76 12.48 -1.12 4.01 39.16 32.88 27.61 -8.09
Cytoplasmic Foci 6.67 9.31 9.11 8.55 -4.26 -4.20 12.40 11.36 12.58 -3.67 -1.08 14.97 13.76 14.82 -4.73
Eisosomes 2.12 5.47 1.99 -0.58 -9.58 -9.31 -3.62 -5.71 -3.22 -5.27 -9.27 -1.36 -12.10 -1.38 -12.25
Endoplasmic Reticulum -7.70 -9.13 -11.97 -2.02 -3.86 -8.78 -16.57 -6.35 -4.10 -3.16 -12.35 -20.51 -17.58 -5.28 -4.09
Endosome -2.16 3.46 -2.17 -0.75 -3.62 -5.21 4.34 -2.85 2.74 -3.96 -6.08 4.77 -3.10 0.62 -3.82
Golgi -2.06 0.60 -1.89 -1.66 -1.93 -1.93 1.41 -1.28 1.74 -4.75 -2.00 1.35 -1.80 -0.57 -1.96
Lipid Particles 2.41 5.26 2.71 0.65 -9.61 -3.75 6.05 -2.53 2.38 -6.00 -3.79 7.68 -2.34 2.62 -10.35
Mitochondria -1.83 -11.67 -23.88 -0.65 -8.45 -2.93 -2.79 -3.15 1.53 -1.28 -3.03 -3.11 -6.57 0.01 -10.00
Mitotic Spindle -1.55 -1.83 -9.82 -8.68 -9.08 -4.08 -5.75 -2.52 -2.16 -2.01 -4.44 -6.19 -10.40 -8.77 -8.44
None -1.60 3.72 -0.45 1.73 -10.61 2.19 7.13 1.76 0.58 -1.42 2.46 8.07 4.96 3.09 -8.02
Nuclear Periphery -1.89 -2.96 -15.39 -16.35 -15.51 -1.44 -12.89 -5.76 -4.04 -2.78 -1.60 -12.29 -17.37 -11.69 -12.44
Nuclear Periphery Foci -2.43 1.13 -2.92 -1.32 -4.32 -3.72 3.55 -1.97 1.60 -3.39 -5.05 3.97 -3.64 -1.29 -4.42
Nucleolus -2.79 -0.16 -4.68 -0.47 -4.49 -2.34 3.35 1.88 3.52 -1.72 -2.32 3.14 -1.04 1.68 -5.46
Nucleus -6.88 -16.53 -7.70 -1.14 21.17 3.78 -24.25 -0.16 -1.29 4.98 0.47 -31.23 -4.72 -5.00 19.08
Peroxisomes -1.55 3.57 2.39 2.77 -2.08 -4.08 3.33 1.78 4.51 -3.52 -4.38 3.49 1.79 5.03 -3.09
Vacuole -0.35 1.49 -8.65 -12.51 -15.56 -8.00 -6.94 -4.40 -3.78 -4.01 -8.71 -4.76 -16.44 -13.31 -15.24
Vacuole Periphery -2.00 0.03 -2.07 -1.42 -2.08 -4.82 -0.33 -2.38 2.70 -0.78 -4.08 -0.21 -2.05 -1.47 -2.02
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Cullin; structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation; human homolog CUL1 can complement yeast cdc53 null mutant
Localization
Cell Percentages nucleus (42%), cytoplasm (9%), mixed (47%)
Cell Cycle Regulation Yes
cytoplasm - MA (1.1e-06)
nucleus - T/G1 (1.1e-06)
Subcompartmental Group N/A

Cdc53

Cdc53


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc53-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available