Standard name | |
---|---|
Human Ortholog | |
Description | Cullin; structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation; human homolog CUL1 can complement yeast cdc53 null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Bud | 0 | 0 | 0 | 2 | 0 | 0 | 4 | – | 3 | 0 | 3 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 6 | 15 | 3 | 24 | 18 | 27 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 4 | 9 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 2 | 2 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 0 | 1 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 29 | 46 | 25 | 15 | 61 | 69 | 101 | – | 75 | 75 | 74 | 56 | 42 | 73 | 84 | 100 | 83 | 64 | 16 | 31 | 20 | 18 | 35 | 38 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 1 | 1 | – | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 17 | 11 | 11 | 0 | 1 | 1 | 1 | 8 | 13 |
Endosome | 3 | 2 | 0 | 0 | 0 | 4 | 1 | – | 1 | 0 | 0 | 0 | 0 | 2 | 13 | 6 | 4 | 9 | 6 | 4 | 2 | 3 | 9 | 15 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 2 | 0 | 0 | 3 | 2 |
Mitochondria | 3 | 5 | 4 | 10 | 4 | 28 | 48 | – | 54 | 49 | 67 | 53 | 18 | 4 | 5 | 4 | 1 | 1 | 2 | 2 | 5 | 1 | 10 | 13 |
Nucleus | 115 | 192 | 131 | 39 | 52 | 24 | 31 | – | 18 | 13 | 14 | 11 | 73 | 78 | 54 | 86 | 57 | 35 | 165 | 290 | 137 | 142 | 116 | 141 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 0 | 0 | 4 | 4 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 3 | 4 | 1 | 3 | 4 | 8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 8 | 4 | 4 | 8 | 17 |
Vac/Vac Membrane | 109 | 194 | 27 | 25 | 26 | 35 | 26 | – | 16 | 10 | 3 | 7 | 87 | 145 | 134 | 167 | 154 | 105 | 28 | 61 | 28 | 74 | 127 | 134 |
Unique Cell Count | 219 | 375 | 169 | 79 | 132 | 145 | 188 | 135 | 123 | 133 | 113 | 198 | 275 | 260 | 317 | 265 | 182 | 244 | 428 | 210 | 279 | 365 | 447 | |
Labelled Cell Count | 259 | 439 | 188 | 91 | 145 | 161 | 214 | 170 | 150 | 165 | 133 | 222 | 303 | 293 | 382 | 313 | 228 | 244 | 428 | 210 | 279 | 365 | 447 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.3 | 7.9 | 6.6 | 6.2 | 6.8 | 6.0 | 5.3 | – | 4.7 | 4.5 | 4.0 | 4.0 | 6.5 | 7.6 | 7.8 | 10.6 | 10.8 | 10.1 | 6.1 | 6.4 | 7.0 |
Std Deviation (1e-4) | 1.1 | 1.4 | 0.9 | 1.2 | 1.6 | 1.9 | 1.3 | – | 1.3 | 1.5 | 1.5 | 1.0 | 1.1 | 1.4 | 1.6 | 2.4 | 2.2 | 2.1 | 1.2 | 1.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.06 | -0.14 | -0.3 | – | -0.49 | -0.55 | -0.71 | -0.73 | -0.01 | 0.21 | 0.25 | 0.7 | 0.72 | 0.62 | -0.1 | -0.05 | 0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.8 | 6.0 | 6.3 | 7.7 | – | 7.5 | 8.2 | 7.5 | 6.3 | 1.6 | 2.9 | 4.1 | 4.0 | 3.9 | 4.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 3.2 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.9 | 6.2 | – | 0 | 0 | 0 | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -4.4 | -6.7 | -10.8 | -11.6 | – | -11.1 | -11.3 | -11.6 | -11.2 | -7.8 | -10.1 | -11.6 | -10.6 | -11.5 | -10.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.8 | 0.8 | 1.8 | -0.6 | – | -1.0 | -2.0 | -4.0 | -2.5 | 5.8 | 7.7 | 7.4 | 7.9 | 8.7 | 8.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.3815 | 5.1832 | 4.6097 | 4.0205 | 4.4241 | 4.9496 | 3.1037 | 4.0521 | 3.2731 | 2.8378 | 3.0084 | 3.3988 | 3.131 | 3.6324 | 3.0818 | 2.9081 | 3.2741 | 3.1912 |
Actin | 0.0766 | 0.0002 | 0.0107 | 0.0005 | 0.004 | 0.0041 | 0.0384 | 0.0001 | 0.0081 | 0.0304 | 0.0008 | 0.0037 | 0.0177 | 0.0001 | 0.01 | 0.0002 | 0.0469 | 0.0073 |
Bud | 0.0008 | 0.0001 | 0.0007 | 0.0005 | 0.0002 | 0.0003 | 0.001 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0001 |
Bud Neck | 0.0068 | 0.0006 | 0.0025 | 0.0008 | 0.0029 | 0.0048 | 0.005 | 0.0002 | 0.0008 | 0.0016 | 0.0026 | 0.0041 | 0.0072 | 0.0004 | 0.0006 | 0.001 | 0.0017 | 0.0021 |
Bud Periphery | 0.0011 | 0.0001 | 0.0008 | 0.0011 | 0.0003 | 0.0003 | 0.0017 | 0 | 0.0002 | 0.0004 | 0.0012 | 0.0003 | 0.0005 | 0 | 0.0004 | 0 | 0.0009 | 0.0002 |
Bud Site | 0.0124 | 0.0009 | 0.0039 | 0.0011 | 0.0006 | 0.0016 | 0.0056 | 0.0003 | 0.0016 | 0.0009 | 0.0004 | 0.0003 | 0.003 | 0.0003 | 0.0003 | 0.0003 | 0.0013 | 0.0003 |
Cell Periphery | 0.0005 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0716 | 0.089 | 0.0803 | 0.0629 | 0.0388 | 0.0542 | 0.0405 | 0.0771 | 0.0567 | 0.0762 | 0.0959 | 0.0347 | 0.0322 | 0.0342 | 0.0329 | 0.0899 | 0.1174 | 0.0374 |
Cytoplasmic Foci | 0.0261 | 0.0005 | 0.0057 | 0.0024 | 0.0213 | 0.0072 | 0.0189 | 0.0002 | 0.007 | 0.0071 | 0.0006 | 0.0025 | 0.0154 | 0.0002 | 0.0004 | 0.0289 | 0.0061 | 0.0022 |
Eisosomes | 0.0006 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.001 | 0.0001 |
Endoplasmic Reticulum | 0.0048 | 0.0039 | 0.0063 | 0.0016 | 0.0045 | 0.0051 | 0.016 | 0.0009 | 0.0067 | 0.0041 | 0.0026 | 0.0019 | 0.0064 | 0.001 | 0.0012 | 0.0013 | 0.0048 | 0.0008 |
Endosome | 0.0243 | 0.0017 | 0.0089 | 0.0292 | 0.0397 | 0.0132 | 0.0436 | 0.0005 | 0.0218 | 0.0126 | 0.0055 | 0.0052 | 0.02 | 0.0006 | 0.0018 | 0.0069 | 0.0158 | 0.0024 |
Golgi | 0.0086 | 0 | 0.0021 | 0.0045 | 0.008 | 0.0049 | 0.0094 | 0 | 0.0079 | 0.0064 | 0.0002 | 0.0027 | 0.0035 | 0 | 0.0008 | 0.0008 | 0.0063 | 0.0009 |
Lipid Particles | 0.0139 | 0.0001 | 0.0019 | 0.0023 | 0.0613 | 0.0042 | 0.0096 | 0 | 0.0025 | 0.001 | 0.0003 | 0.0034 | 0.0041 | 0 | 0.0012 | 0.0034 | 0.0045 | 0.0006 |
Mitochondria | 0.0142 | 0.0003 | 0.0094 | 0.0257 | 0.0041 | 0.0144 | 0.0078 | 0.0002 | 0.0032 | 0.0022 | 0.0015 | 0.0013 | 0.0012 | 0.0002 | 0.0107 | 0.0003 | 0.0028 | 0.0039 |
None | 0.0444 | 0.014 | 0.0326 | 0.0621 | 0.0316 | 0.0169 | 0.0169 | 0.0004 | 0.0015 | 0.0081 | 0.0011 | 0.0004 | 0.0087 | 0.0012 | 0.0123 | 0.0028 | 0.0037 | 0.0013 |
Nuclear Periphery | 0.022 | 0.0232 | 0.0165 | 0.0089 | 0.0282 | 0.0118 | 0.0496 | 0.0183 | 0.0262 | 0.0296 | 0.0474 | 0.017 | 0.0214 | 0.0095 | 0.0114 | 0.0143 | 0.035 | 0.0078 |
Nucleolus | 0.0058 | 0.0024 | 0.0034 | 0.0024 | 0.006 | 0.0072 | 0.0027 | 0.0019 | 0.0019 | 0.0024 | 0.0062 | 0.0028 | 0.0026 | 0.0012 | 0.002 | 0.0017 | 0.0025 | 0.0022 |
Nucleus | 0.61 | 0.853 | 0.7878 | 0.7757 | 0.6946 | 0.828 | 0.7012 | 0.8902 | 0.8416 | 0.8016 | 0.8218 | 0.9067 | 0.8349 | 0.945 | 0.9085 | 0.7952 | 0.731 | 0.9188 |
Peroxisomes | 0.0265 | 0 | 0.0035 | 0.0005 | 0.0134 | 0.002 | 0.0085 | 0 | 0.0008 | 0.0005 | 0.0003 | 0.0031 | 0.0043 | 0 | 0.0008 | 0.0048 | 0.003 | 0.0012 |
Punctate Nuclear | 0.0227 | 0.0046 | 0.0121 | 0.0073 | 0.0328 | 0.0166 | 0.0106 | 0.0083 | 0.0074 | 0.0106 | 0.0067 | 0.0078 | 0.0135 | 0.0052 | 0.0024 | 0.047 | 0.0117 | 0.0095 |
Vacuole | 0.0053 | 0.0048 | 0.0085 | 0.0078 | 0.0064 | 0.0026 | 0.008 | 0.001 | 0.0032 | 0.0031 | 0.0035 | 0.0014 | 0.0024 | 0.0006 | 0.001 | 0.001 | 0.0018 | 0.0007 |
Vacuole Periphery | 0.001 | 0.0004 | 0.0019 | 0.0026 | 0.0012 | 0.0005 | 0.0043 | 0.0002 | 0.0006 | 0.0007 | 0.001 | 0.0004 | 0.0006 | 0.0001 | 0.0006 | 0.0002 | 0.0009 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 36.7351 | 37.4726 | 38.271 | 54.6072 | 38.5704 | 39.1924 | 42.7774 | 51.5569 | 48.0832 | 46.8725 |
Translational Efficiency | 0.9422 | 0.9982 | 1.0553 | 0.6714 | 0.7977 | 1.0044 | 0.988 | 0.7207 | 0.8591 | 0.8456 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
233 | 920 | 1225 | 1289 | 2320 | 1562 | 1982 | 71 | 2553 | 2482 | 3207 | 1360 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 668.22 | 842.57 | 1240.37 | 1422.27 | 755.95 | 790.85 | 1272.37 | 1469.49 | 747.94 | 810.02 | 1260.15 | 1424.74 |
Standard Deviation | 69.51 | 123.09 | 170.89 | 203.35 | 118.27 | 112.42 | 180.65 | 231.80 | 117.44 | 119.14 | 177.67 | 205.20 |
Intensity Change Log 2 | — | 0.334473 | 0.892375 | 1.089800 | — | 0.065113 | 0.751156 | 0.958953 | — | 0.197774 | 0.819140 | 1.021826 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000082 | 0.000310 | 0.000562 | 0.001006 | 0.000075 | 0.000276 | 0.000306 | 0.000799 | 0.000075 | 0.000288 | 0.000404 | 0.000995 |
Bud Neck | 0.000895 | 0.002475 | 0.013118 | 0.017363 | 0.002029 | 0.004887 | 0.014734 | 0.007868 | 0.001925 | 0.003993 | 0.014117 | 0.016868 |
Bud Site | 0.001670 | 0.001455 | 0.001396 | 0.008625 | 0.000878 | 0.003396 | 0.001722 | 0.002299 | 0.000950 | 0.002677 | 0.001597 | 0.008295 |
Cell Periphery | 0.000127 | 0.000147 | 0.000114 | 0.000189 | 0.000102 | 0.000159 | 0.000102 | 0.000178 | 0.000104 | 0.000155 | 0.000106 | 0.000189 |
Cytoplasm | 0.299974* | 0.173156* | 0.012954 | 0.061460 | 0.208654* | 0.203424* | 0.041846 | 0.052338 | 0.216988* | 0.192205* | 0.030810 | 0.060984 |
Cytoplasmic Foci | 0.045136 | 0.011486 | 0.000137 | 0.003765 | 0.011841 | 0.017969 | 0.000070 | 0.000290 | 0.014879 | 0.015566 | 0.000095 | 0.003583 |
Eisosomes | 0.000045 | 0.000032 | 0.000016 | 0.000020 | 0.000015 | 0.000033 | 0.000014 | 0.000021 | 0.000018 | 0.000033 | 0.000015 | 0.000020 |
Endoplasmic Reticulum | 0.001125 | 0.003824 | 0.003038 | 0.003448 | 0.001205 | 0.003033 | 0.003365 | 0.004369 | 0.001198 | 0.003326 | 0.003240 | 0.003496 |
Endosome | 0.000765 | 0.001262 | 0.000251 | 0.006817 | 0.000515 | 0.002070 | 0.000241 | 0.000439 | 0.000538 | 0.001770 | 0.000245 | 0.006484 |
Golgi | 0.000093 | 0.000223 | 0.000056 | 0.007693 | 0.000158 | 0.001186 | 0.000035 | 0.000087 | 0.000152 | 0.000829 | 0.000043 | 0.007296 |
Lipid Particles | 0.002319 | 0.001242 | 0.000130 | 0.000980 | 0.000753 | 0.002207 | 0.000084 | 0.000352 | 0.000896 | 0.001850 | 0.000102 | 0.000947 |
Mitochondria | 0.000363 | 0.002892 | 0.001714 | 0.003268 | 0.001081 | 0.004124 | 0.001681 | 0.001450 | 0.001016 | 0.003667 | 0.001694 | 0.003173 |
Mitotic Spindle | 0.000453 | 0.004343 | 0.002060 | 0.101344* | 0.000600 | 0.007628 | 0.008330 | 0.107927* | 0.000587 | 0.006410 | 0.005935 | 0.101687* |
None | 0.006177 | 0.008314 | 0.002540 | 0.006330 | 0.012288 | 0.009243 | 0.004024 | 0.006537 | 0.011730 | 0.008899 | 0.003457 | 0.006341 |
Nuclear Periphery | 0.000695 | 0.000974 | 0.000890 | 0.002312 | 0.000978 | 0.001501 | 0.001765 | 0.001399 | 0.000952 | 0.001306 | 0.001431 | 0.002264 |
Nuclear Periphery Foci | 0.000394 | 0.001136 | 0.000161 | 0.004362 | 0.000425 | 0.001122 | 0.000124 | 0.000226 | 0.000422 | 0.001127 | 0.000138 | 0.004146 |
Nucleolus | 0.001001 | 0.001928 | 0.000740 | 0.000876 | 0.001813 | 0.002895 | 0.000513 | 0.000339 | 0.001739 | 0.002536 | 0.000600 | 0.000848 |
Nucleus | 0.631446* | 0.773509* | 0.956970* | 0.744965* | 0.752485* | 0.720970* | 0.916390* | 0.801029* | 0.741438* | 0.740444* | 0.931891* | 0.747891* |
Peroxisomes | 0.000528 | 0.000991 | 0.000067 | 0.000563 | 0.000406 | 0.002833 | 0.000024 | 0.000066 | 0.000417 | 0.002150 | 0.000040 | 0.000537 |
Vacuole | 0.006612 | 0.009981 | 0.003007 | 0.019236 | 0.003586 | 0.010732 | 0.004424 | 0.011904 | 0.003862 | 0.010453 | 0.003883 | 0.018853 |
Vacuole Periphery | 0.000099 | 0.000322 | 0.000080 | 0.005379 | 0.000115 | 0.000314 | 0.000206 | 0.000082 | 0.000113 | 0.000317 | 0.000158 | 0.005102 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.06 | -12.89 | -12.87 | -9.27 | -5.80 | -6.71 | -16.07 | -7.95 | -6.73 | -5.86 | -7.78 | -21.24 | -14.65 | -11.36 | -8.57 |
Bud Neck | -4.56 | -12.39 | -17.92 | -16.23 | -4.65 | -4.06 | -14.58 | -4.52 | -3.97 | -1.78 | -4.45 | -18.50 | -17.72 | -15.26 | -5.27 |
Bud Site | 0.27 | 0.01 | -3.69 | -7.61 | -7.29 | -4.14 | -5.59 | -4.24 | -2.91 | -3.53 | -4.11 | -5.12 | -8.89 | -6.22 | -7.46 |
Cell Periphery | -1.16 | 0.66 | -5.99 | -4.97 | -8.55 | -4.02 | -0.45 | -4.94 | -3.98 | -4.90 | -4.73 | -0.98 | -11.47 | -6.03 | -12.06 |
Cytoplasm | 6.93 | 16.92 | 14.86 | 16.35 | -13.85 | 1.00 | 33.50 | 13.76 | 12.48 | -1.12 | 4.01 | 39.16 | 32.88 | 27.61 | -8.09 |
Cytoplasmic Foci | 6.67 | 9.31 | 9.11 | 8.55 | -4.26 | -4.20 | 12.40 | 11.36 | 12.58 | -3.67 | -1.08 | 14.97 | 13.76 | 14.82 | -4.73 |
Eisosomes | 2.12 | 5.47 | 1.99 | -0.58 | -9.58 | -9.31 | -3.62 | -5.71 | -3.22 | -5.27 | -9.27 | -1.36 | -12.10 | -1.38 | -12.25 |
Endoplasmic Reticulum | -7.70 | -9.13 | -11.97 | -2.02 | -3.86 | -8.78 | -16.57 | -6.35 | -4.10 | -3.16 | -12.35 | -20.51 | -17.58 | -5.28 | -4.09 |
Endosome | -2.16 | 3.46 | -2.17 | -0.75 | -3.62 | -5.21 | 4.34 | -2.85 | 2.74 | -3.96 | -6.08 | 4.77 | -3.10 | 0.62 | -3.82 |
Golgi | -2.06 | 0.60 | -1.89 | -1.66 | -1.93 | -1.93 | 1.41 | -1.28 | 1.74 | -4.75 | -2.00 | 1.35 | -1.80 | -0.57 | -1.96 |
Lipid Particles | 2.41 | 5.26 | 2.71 | 0.65 | -9.61 | -3.75 | 6.05 | -2.53 | 2.38 | -6.00 | -3.79 | 7.68 | -2.34 | 2.62 | -10.35 |
Mitochondria | -1.83 | -11.67 | -23.88 | -0.65 | -8.45 | -2.93 | -2.79 | -3.15 | 1.53 | -1.28 | -3.03 | -3.11 | -6.57 | 0.01 | -10.00 |
Mitotic Spindle | -1.55 | -1.83 | -9.82 | -8.68 | -9.08 | -4.08 | -5.75 | -2.52 | -2.16 | -2.01 | -4.44 | -6.19 | -10.40 | -8.77 | -8.44 |
None | -1.60 | 3.72 | -0.45 | 1.73 | -10.61 | 2.19 | 7.13 | 1.76 | 0.58 | -1.42 | 2.46 | 8.07 | 4.96 | 3.09 | -8.02 |
Nuclear Periphery | -1.89 | -2.96 | -15.39 | -16.35 | -15.51 | -1.44 | -12.89 | -5.76 | -4.04 | -2.78 | -1.60 | -12.29 | -17.37 | -11.69 | -12.44 |
Nuclear Periphery Foci | -2.43 | 1.13 | -2.92 | -1.32 | -4.32 | -3.72 | 3.55 | -1.97 | 1.60 | -3.39 | -5.05 | 3.97 | -3.64 | -1.29 | -4.42 |
Nucleolus | -2.79 | -0.16 | -4.68 | -0.47 | -4.49 | -2.34 | 3.35 | 1.88 | 3.52 | -1.72 | -2.32 | 3.14 | -1.04 | 1.68 | -5.46 |
Nucleus | -6.88 | -16.53 | -7.70 | -1.14 | 21.17 | 3.78 | -24.25 | -0.16 | -1.29 | 4.98 | 0.47 | -31.23 | -4.72 | -5.00 | 19.08 |
Peroxisomes | -1.55 | 3.57 | 2.39 | 2.77 | -2.08 | -4.08 | 3.33 | 1.78 | 4.51 | -3.52 | -4.38 | 3.49 | 1.79 | 5.03 | -3.09 |
Vacuole | -0.35 | 1.49 | -8.65 | -12.51 | -15.56 | -8.00 | -6.94 | -4.40 | -3.78 | -4.01 | -8.71 | -4.76 | -16.44 | -13.31 | -15.24 |
Vacuole Periphery | -2.00 | 0.03 | -2.07 | -1.42 | -2.08 | -4.82 | -0.33 | -2.38 | 2.70 | -0.78 | -4.08 | -0.21 | -2.05 | -1.47 | -2.02 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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