Standard name
Human Ortholog
Description Cullin; structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation; human homolog CUL1 can complement yeast cdc53 null mutant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0.12 0.15 0.19 0.46 0.48 0.54 0.56 0.61 0.56 0.5 0.21 0.27 0.32 0.32 0.31 0.35 0.07 0.07 0.1 0.06 0.1 0.09
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.13 0 0.19 0.26 0.4 0.4 0.5 0.47 0.09 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.53 0.51 0.78 0.49 0.39 0.17 0.16 0.13 0.11 0.11 0.1 0.37 0.28 0.21 0.27 0.22 0.19 0.68 0.68 0.66 0.51 0.32 0.32
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.5 0.52 0.16 0.32 0.2 0.24 0.14 0.12 0.08 0 0.06 0.44 0.53 0.52 0.53 0.58 0.58 0.12 0.14 0.14 0.27 0.35 0.3
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 2
Bud 0 0 0 2 0 0 4 3 0 3 1 0 0 1 0 0 0 6 15 3 24 18 27
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 4 9
Bud Site 0 0 0 0 0 0 2 2 2 3 2 0 0 0 0 0 0
Cell Periphery 0 0 1 0 1 0 0 0 1 0 1 0 0 1 0 1 1 0 0 0 0 0 0
Cytoplasm 29 46 25 15 61 69 101 75 75 74 56 42 73 84 100 83 64 16 31 20 18 35 38
Endoplasmic Reticulum 0 0 0 0 1 1 1 0 0 0 0 1 1 0 17 11 11 0 1 1 1 8 13
Endosome 3 2 0 0 0 4 1 1 0 0 0 0 2 13 6 4 9 6 4 2 3 9 15
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 2 2 0 0 3 2
Mitochondria 3 5 4 10 4 28 48 54 49 67 53 18 4 5 4 1 1 2 2 5 1 10 13
Nucleus 115 192 131 39 52 24 31 18 13 14 11 73 78 54 86 57 35 165 290 137 142 116 141
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 4 1 0 0 4 4
Nucleolus 0 0 0 0 0 0 0 0 0 1 1 1 0 1 0 0 0 3 4 1 3 4 8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 8 4 4 8 17
Vac/Vac Membrane 109 194 27 25 26 35 26 16 10 3 7 87 145 134 167 154 105 28 61 28 74 127 134
Unique Cell Count 219 375 169 79 132 145 188 135 123 133 113 198 275 260 317 265 182 244 428 210 279 365 447
Labelled Cell Count 259 439 188 91 145 161 214 170 150 165 133 222 303 293 382 313 228 244 428 210 279 365 447


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 7.9 6.6 6.2 6.8 6.0 5.3 4.7 4.5 4.0 4.0 6.5 7.6 7.8 10.6 10.8 10.1 6.1 6.4 7.0
Std Deviation (1e-4) 1.1 1.4 0.9 1.2 1.6 1.9 1.3 1.3 1.5 1.5 1.0 1.1 1.4 1.6 2.4 2.2 2.1 1.2 1.5 1.6
Intensity Change (Log2) -0.09 0.06 -0.14 -0.3 -0.49 -0.55 -0.71 -0.73 -0.01 0.21 0.25 0.7 0.72 0.62 -0.1 -0.05 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 6.0 6.3 7.7 7.5 8.2 7.5 6.3 1.6 2.9 4.1 4.0 3.9 4.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 3.1 0 3.2
Endosome 0 0 0 0 0 0 0 0 0 0 3.0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.9 6.2 0 0 0 0 2.7 0 0 0 0 0
Nucleus -4.4 -6.7 -10.8 -11.6 -11.1 -11.3 -11.6 -11.2 -7.8 -10.1 -11.6 -10.6 -11.5 -10.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.8 0.8 1.8 -0.6 -1.0 -2.0 -4.0 -2.5 5.8 7.7 7.4 7.9 8.7 8.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.3815 5.1832 4.6097 4.0205 4.4241 4.9496 3.1037 4.0521 3.2731 2.8378 3.0084 3.3988 3.131 3.6324 3.0818 2.9081 3.2741 3.1912
Actin 0.0766 0.0002 0.0107 0.0005 0.004 0.0041 0.0384 0.0001 0.0081 0.0304 0.0008 0.0037 0.0177 0.0001 0.01 0.0002 0.0469 0.0073
Bud 0.0008 0.0001 0.0007 0.0005 0.0002 0.0003 0.001 0.0001 0.0003 0.0003 0.0003 0.0002 0.0004 0.0001 0.0002 0.0001 0.0005 0.0001
Bud Neck 0.0068 0.0006 0.0025 0.0008 0.0029 0.0048 0.005 0.0002 0.0008 0.0016 0.0026 0.0041 0.0072 0.0004 0.0006 0.001 0.0017 0.0021
Bud Periphery 0.0011 0.0001 0.0008 0.0011 0.0003 0.0003 0.0017 0 0.0002 0.0004 0.0012 0.0003 0.0005 0 0.0004 0 0.0009 0.0002
Bud Site 0.0124 0.0009 0.0039 0.0011 0.0006 0.0016 0.0056 0.0003 0.0016 0.0009 0.0004 0.0003 0.003 0.0003 0.0003 0.0003 0.0013 0.0003
Cell Periphery 0.0005 0.0001 0.0004 0.0002 0.0001 0.0001 0.0003 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0
Cytoplasm 0.0716 0.089 0.0803 0.0629 0.0388 0.0542 0.0405 0.0771 0.0567 0.0762 0.0959 0.0347 0.0322 0.0342 0.0329 0.0899 0.1174 0.0374
Cytoplasmic Foci 0.0261 0.0005 0.0057 0.0024 0.0213 0.0072 0.0189 0.0002 0.007 0.0071 0.0006 0.0025 0.0154 0.0002 0.0004 0.0289 0.0061 0.0022
Eisosomes 0.0006 0 0.0002 0 0.0001 0.0001 0.0003 0 0 0.0001 0 0 0.0001 0 0.0006 0 0.001 0.0001
Endoplasmic Reticulum 0.0048 0.0039 0.0063 0.0016 0.0045 0.0051 0.016 0.0009 0.0067 0.0041 0.0026 0.0019 0.0064 0.001 0.0012 0.0013 0.0048 0.0008
Endosome 0.0243 0.0017 0.0089 0.0292 0.0397 0.0132 0.0436 0.0005 0.0218 0.0126 0.0055 0.0052 0.02 0.0006 0.0018 0.0069 0.0158 0.0024
Golgi 0.0086 0 0.0021 0.0045 0.008 0.0049 0.0094 0 0.0079 0.0064 0.0002 0.0027 0.0035 0 0.0008 0.0008 0.0063 0.0009
Lipid Particles 0.0139 0.0001 0.0019 0.0023 0.0613 0.0042 0.0096 0 0.0025 0.001 0.0003 0.0034 0.0041 0 0.0012 0.0034 0.0045 0.0006
Mitochondria 0.0142 0.0003 0.0094 0.0257 0.0041 0.0144 0.0078 0.0002 0.0032 0.0022 0.0015 0.0013 0.0012 0.0002 0.0107 0.0003 0.0028 0.0039
None 0.0444 0.014 0.0326 0.0621 0.0316 0.0169 0.0169 0.0004 0.0015 0.0081 0.0011 0.0004 0.0087 0.0012 0.0123 0.0028 0.0037 0.0013
Nuclear Periphery 0.022 0.0232 0.0165 0.0089 0.0282 0.0118 0.0496 0.0183 0.0262 0.0296 0.0474 0.017 0.0214 0.0095 0.0114 0.0143 0.035 0.0078
Nucleolus 0.0058 0.0024 0.0034 0.0024 0.006 0.0072 0.0027 0.0019 0.0019 0.0024 0.0062 0.0028 0.0026 0.0012 0.002 0.0017 0.0025 0.0022
Nucleus 0.61 0.853 0.7878 0.7757 0.6946 0.828 0.7012 0.8902 0.8416 0.8016 0.8218 0.9067 0.8349 0.945 0.9085 0.7952 0.731 0.9188
Peroxisomes 0.0265 0 0.0035 0.0005 0.0134 0.002 0.0085 0 0.0008 0.0005 0.0003 0.0031 0.0043 0 0.0008 0.0048 0.003 0.0012
Punctate Nuclear 0.0227 0.0046 0.0121 0.0073 0.0328 0.0166 0.0106 0.0083 0.0074 0.0106 0.0067 0.0078 0.0135 0.0052 0.0024 0.047 0.0117 0.0095
Vacuole 0.0053 0.0048 0.0085 0.0078 0.0064 0.0026 0.008 0.001 0.0032 0.0031 0.0035 0.0014 0.0024 0.0006 0.001 0.001 0.0018 0.0007
Vacuole Periphery 0.001 0.0004 0.0019 0.0026 0.0012 0.0005 0.0043 0.0002 0.0006 0.0007 0.001 0.0004 0.0006 0.0001 0.0006 0.0002 0.0009 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.7351 37.4726 38.271 54.6072 38.5704 39.1924 42.7774 51.5569 48.0832 46.8725
Translational Efficiency 0.9422 0.9982 1.0553 0.6714 0.7977 1.0044 0.988 0.7207 0.8591 0.8456

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Cullin; structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation; human homolog CUL1 can complement yeast cdc53 null mutant
Localization
Cell Percentages nucleus (42%), cytoplasm (9%), mixed (47%)
Cell Cycle Regulation Yes
cytoplasm - MA (1.1e-06)
nucleus - T/G1 (1.1e-06)
Subcompartmental Group N/A

Cdc53

Cdc53


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc53-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available