Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0.08 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 – 1.0 1.0 0.96 0.94 0.79 0.74 0.77 0.75 0.74 0.68 0.98 1.0 0.99 0.99 0.97 0.98 0.96 0.89 0.92 0.87 0.96 0.94
Endoplasmic Reticulum 0 – 0 0 0.05 0.05 0.18 0.22 0.19 0.16 0.19 0.22 0 0 0 0.06 0.06 0.09 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 4 6 10 15 12 30 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 2 0 0 0 1 4 0 2 1 0 0 1 0 0 0 0 4 0 2 0 0 1
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 – 0 1 2 9 16 19 15 36 17 31 0 1 3 2 1 2 0 0 0 0 0 0
Cytoplasm 438 – 222 374 401 386 429 417 302 348 342 336 86 176 153 176 138 160 426 41 201 19 146 118
Endoplasmic Reticulum 2 – 0 1 19 21 96 127 73 73 87 109 1 0 0 10 8 15 2 0 4 0 2 2
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 9 3 12 11 8 16 0 0 1 0 1 0 0 0 1 0 0 0
Mitochondria 0 – 1 0 0 0 1 1 7 11 15 28 1 0 0 1 3 1 0 1 1 0 0 0
Nucleus 0 – 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 – 0 0 0 0 0 3 1 1 2 1 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 1 4 0 3 1 2 1 0 1 0 1 0 3 0 2 0 1 1
Unique Cell Count 442 223 375 419 410 541 565 393 461 463 495 88 176 155 177 142 163 447 47 219 22 153 126
Labelled Cell Count 442 225 376 422 416 558 584 421 500 485 553 90 178 158 189 152 178 447 47 219 22 153 126


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 179.0 – 103.2 145.0 118.0 83.4 68.1 62.0 57.9 51.5 48.9 45.5 173.1 177.2 165.3 259.0 237.9 241.8 – 211.2 211.5
Std Deviation (1e-4) 39.5 – 31.9 26.3 22.9 15.8 16.1 14.9 13.7 13.0 10.8 10.8 37.2 39.4 36.0 56.0 77.2 67.3 – 40.6 48.4
Intensity Change (Log2) – – – 0.49 0.19 -0.31 -0.6 -0.74 -0.83 -1.0 -1.08 -1.18 0.75 0.78 0.68 1.33 1.2 1.23 – 1.03 1.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 3.8 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 2.8 3.0 4.3 2.9 3.8 0 0 0 0 0 0
Cytoplasm 0.4 -2.7 -3.3 -7.2 -8.3 -7.6 -7.9 -8.2 -9.4 -1.5 0.9 -0.9 -0.2 -1.9 -1.3
Endoplasmic Reticulum 0 3.2 3.4 6.7 7.7 6.9 6.3 6.9 7.6 0 0 0 0 0 4.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 2.3 3.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 184.2252 253.9436 223.0844 219.3716 204.7563 106.1831 191.4682 225.4407 203.9917 191.9626 199.3631 213.8326 212.4937 281.701 246.9897 239.7586 160.4199 221.3297
Actin 0.0042 0.0002 0.0006 0.0017 0.0041 0.0002 0.0035 0.001 0.0017 0.0095 0.0004 0.0003 0.0017 0.0003 0.0172 0.0004 0.0059 0.001
Bud 0.0018 0.0008 0.0013 0.0018 0.005 0.0016 0.0023 0.0024 0.0027 0.0096 0.0029 0.0008 0.003 0.0014 0.002 0.0013 0.0032 0.0017
Bud Neck 0.0008 0.0009 0.001 0.0008 0.0016 0.0008 0.0009 0.0006 0.001 0.0008 0.0011 0.0008 0.0009 0.0005 0.0011 0.0009 0.0012 0.0005
Bud Periphery 0.0031 0.0008 0.0012 0.0017 0.0052 0.0023 0.0025 0.0012 0.0012 0.0225 0.0012 0.0002 0.0029 0.0005 0.0012 0.0008 0.0027 0.0008
Bud Site 0.0008 0.0009 0.0006 0.0007 0.0254 0.0004 0.001 0.0013 0.0011 0.002 0.0008 0 0.001 0.0007 0.0015 0.0005 0.0018 0.0002
Cell Periphery 0.0572 0.0625 0.0395 0.0299 0.0075 0.012 0.036 0.0199 0.0181 0.0182 0.0059 0.0002 0.03 0.014 0.0109 0.0442 0.0046 0.0025
Cytoplasm 0.5541 0.7464 0.6942 0.549 0.6007 0.2775 0.645 0.8701 0.7643 0.6399 0.6645 0.954 0.6509 0.8972 0.7745 0.6334 0.5664 0.722
Cytoplasmic Foci 0.0178 0.0007 0.0061 0.0012 0.0033 0.0033 0.0027 0.0018 0.0032 0.0009 0.0027 0.0017 0.0108 0.0008 0.0083 0.0013 0.0192 0.0011
Eisosomes 0.0006 0 0.0005 0.0003 0.0001 0.0004 0.0007 0 0.0005 0.0001 0 0 0.0003 0.0001 0.0002 0.0001 0.0011 0
Endoplasmic Reticulum 0.0361 0.014 0.0199 0.0031 0.002 0.0065 0.0301 0.01 0.009 0.0051 0.0026 0.005 0.0337 0.002 0.0054 0.0054 0.0048 0.0024
Endosome 0.0081 0.0018 0.0049 0.0034 0.0156 0.0115 0.0068 0.0021 0.0045 0.006 0.0156 0.0058 0.0058 0.0015 0.0031 0.0029 0.0679 0.0026
Golgi 0.0016 0.0004 0.0007 0.0003 0.0034 0.0005 0.0016 0.0007 0.0007 0.0007 0.0011 0.0002 0.0012 0.0002 0.0009 0.0002 0.0226 0.0002
Lipid Particles 0.0068 0.0023 0.0043 0.0011 0.0033 0.0027 0.0024 0.0008 0.0026 0.0007 0.0005 0 0.0026 0.0004 0.0022 0.0025 0.0128 0.0001
Mitochondria 0.0123 0.0011 0.0074 0.0294 0.0057 0.0168 0.0141 0.005 0.0137 0.0089 0.0016 0.0009 0.0226 0.0014 0.006 0.0013 0.0466 0.0033
None 0.0056 0.0002 0.0043 0.0012 0.001 0.0062 0.0023 0.0005 0.0018 0.0004 0.0005 0.0002 0.0042 0.0004 0.0028 0.0021 0.0175 0.0013
Nuclear Periphery 0.0324 0.0087 0.0117 0.0066 0.0134 0.0175 0.0428 0.0036 0.0095 0.0118 0.0079 0.0046 0.0352 0.0034 0.012 0.0283 0.0241 0.0359
Nucleolus 0.0159 0.0006 0.0094 0.0067 0.0042 0.1541 0.0028 0.0006 0.0065 0.0021 0.0015 0 0.0053 0.0011 0.0103 0.0045 0.0406 0.0054
Nucleus 0.0135 0.0038 0.0093 0.0098 0.029 0.0387 0.0142 0.0028 0.0087 0.0067 0.0158 0.004 0.0183 0.004 0.0113 0.0152 0.0116 0.0771
Peroxisomes 0.0015 0 0.0024 0.0004 0.0011 0.0003 0.0002 0.0001 0.0005 0.0001 0.0001 0 0.0008 0.0001 0.0031 0.0001 0.0021 0.0001
Punctate Nuclear 0.0016 0 0.0008 0.0001 0.0007 0.0004 0.0002 0 0.0003 0 0.0001 0 0.0063 0 0.0004 0.0002 0.001 0.0002
Vacuole 0.1907 0.1412 0.1535 0.2745 0.1905 0.2382 0.152 0.0666 0.1225 0.1906 0.2018 0.0151 0.1262 0.0578 0.0922 0.2243 0.084 0.0687
Vacuole Periphery 0.0335 0.0125 0.0267 0.0764 0.0773 0.2081 0.0361 0.0088 0.0257 0.0633 0.0713 0.0061 0.0361 0.0121 0.0334 0.0301 0.0582 0.0728

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8381.6299 10597.2209 15089.0943 7495.2046 13865.9326 14802.8833 9087.7947 9980.3939 14795.6837 7201.948
Translational Efficiency 2.4131 2.1354 1.287 4.1145 1.6052 1.1915 1.9337 2.0407 1.1932 2.1524

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rpl41b

Rpl41b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl41b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available