Standard name | |
---|---|
Human Ortholog | |
Description | Catalytic subunit of protein phosphatase 2A, PP2A; functionally redundant with Pph22p; C-terminally methylated; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 3 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 4 | 3 | 3 | 5 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 6 | 10 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 4 | 0 | 2 | 1 | 3 | 6 | 3 | 5 | 0 | 0 | 0 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 123 | 160 | 102 | 97 | 172 | 167 | 202 | 185 | 139 | 152 | 132 | 155 | 229 | 297 | 335 | 148 | 100 | 105 | 114 | 150 | 134 | 185 | 190 | 157 |
Endoplasmic Reticulum | 1 | 2 | 1 | 0 | 2 | 0 | 1 | 3 | 3 | 3 | 0 | 3 | 1 | 0 | 0 | 9 | 4 | 4 | 1 | 1 | 2 | 0 | 2 | 3 |
Endosome | 0 | 0 | 2 | 2 | 5 | 2 | 0 | 2 | 0 | 1 | 1 | 1 | 3 | 1 | 2 | 15 | 14 | 13 | 1 | 2 | 4 | 2 | 25 | 39 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 7 | 5 | 6 | 2 | 0 | 0 | 1 | 12 | 5 |
Mitochondria | 4 | 1 | 0 | 4 | 7 | 26 | 60 | 46 | 59 | 48 | 65 | 30 | 0 | 0 | 0 | 1 | 3 | 9 | 1 | 1 | 3 | 3 | 6 | 4 |
Nucleus | 0 | 1 | 2 | 3 | 9 | 9 | 7 | 27 | 12 | 9 | 12 | 15 | 10 | 5 | 4 | 10 | 5 | 9 | 4 | 15 | 9 | 24 | 17 | 14 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 12 | 8 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 4 |
SpindlePole | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 5 | 4 | 0 | 0 | 2 | 1 | 24 | 27 |
Vac/Vac Membrane | 2 | 13 | 14 | 90 | 110 | 59 | 71 | 106 | 42 | 74 | 38 | 66 | 89 | 101 | 59 | 170 | 136 | 101 | 3 | 8 | 6 | 9 | 6 | 15 |
Unique Cell Count | 127 | 170 | 115 | 173 | 269 | 243 | 306 | 339 | 222 | 259 | 221 | 247 | 289 | 346 | 365 | 301 | 226 | 218 | 134 | 184 | 169 | 236 | 303 | 300 |
Labelled Cell Count | 131 | 177 | 122 | 200 | 311 | 264 | 350 | 378 | 261 | 300 | 256 | 279 | 335 | 405 | 400 | 382 | 286 | 260 | 134 | 184 | 169 | 236 | 303 | 300 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.8 | 9.8 | 7.6 | 8.4 | 8.4 | 7.3 | 7.0 | 7.4 | 6.8 | 7.2 | 6.8 | 7.6 | 9.0 | 9.5 | 9.6 | 10.9 | 11.9 | 11.5 | 8.7 | 8.6 | 9.2 |
Std Deviation (1e-4) | 0.9 | 2.0 | 1.2 | 1.9 | 1.9 | 1.8 | 1.7 | 1.3 | 1.6 | 1.5 | 1.6 | 1.6 | 1.4 | 1.3 | 1.3 | 2.6 | 2.6 | 2.8 | 1.5 | 1.4 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.14 | 0.14 | -0.07 | -0.12 | -0.05 | -0.15 | -0.07 | -0.17 | -0.01 | 0.24 | 0.32 | 0.34 | 0.52 | 0.64 | 0.59 | 0.19 | 0.17 | 0.28 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -5.9 | -4.9 | -4.1 | -4.6 | -6.6 | -5.0 | -5.7 | -5.5 | -5.1 | -2.2 | -0.8 | 1.0 | -7.4 | -7.9 | -7.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.8 | 1.8 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.6 | 5.1 | 4.2 | 6.1 | 4.9 | 6.5 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 6.9 | 5.5 | 2.7 | 2.5 | 4.0 | 1.6 | 3.4 | 1.2 | 3.1 | 3.9 | 3.7 | 1.0 | 8.1 | 8.4 | 6.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.528 | 6.9575 | 6.066 | 5.9281 | 4.5333 | 6.0194 | 4.2038 | 5.5641 | 5.0511 | 4.4233 | 4.2625 | 5.0375 | 3.6237 | 4.9197 | 3.8897 | 3.6869 | 3.3094 | 4.1266 |
Actin | 0.0102 | 0.0012 | 0.0013 | 0.0004 | 0.0314 | 0.0008 | 0.0311 | 0.0004 | 0.0079 | 0.003 | 0.0029 | 0.0034 | 0.0538 | 0.0002 | 0.0167 | 0.1251 | 0.0242 | 0.0006 |
Bud | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0067 | 0.0001 | 0.0092 | 0.0004 | 0.0051 | 0.001 | 0.0003 | 0.0002 | 0.001 | 0.0001 | 0.0008 | 0.0009 | 0.0002 | 0.0014 |
Bud Neck | 0.0023 | 0.0006 | 0.0011 | 0.0007 | 0.0043 | 0.0021 | 0.0015 | 0.0008 | 0.0028 | 0.0017 | 0.0012 | 0.0024 | 0.0041 | 0.0003 | 0.001 | 0.0031 | 0.0009 | 0.0025 |
Bud Periphery | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0032 | 0.0001 | 0.0034 | 0.0001 | 0.0019 | 0.0003 | 0.0003 | 0.0001 | 0.002 | 0 | 0.0009 | 0.0006 | 0.0002 | 0.001 |
Bud Site | 0.0049 | 0.0016 | 0.0006 | 0.0001 | 0.0039 | 0.0002 | 0.0037 | 0.001 | 0.0023 | 0.0008 | 0.0008 | 0.0002 | 0.0058 | 0.0005 | 0.0145 | 0.002 | 0.0006 | 0.0005 |
Cell Periphery | 0.0004 | 0 | 0 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0006 | 0 | 0.0003 | 0.0002 | 0 | 0.0001 |
Cytoplasm | 0.4974 | 0.5262 | 0.4337 | 0.3189 | 0.4728 | 0.497 | 0.5131 | 0.5882 | 0.5442 | 0.3688 | 0.5397 | 0.518 | 0.6127 | 0.7442 | 0.559 | 0.4914 | 0.3438 | 0.6643 |
Cytoplasmic Foci | 0.0186 | 0.0025 | 0.0105 | 0.0019 | 0.0356 | 0.0066 | 0.0185 | 0.0036 | 0.0127 | 0.0394 | 0.0376 | 0.0057 | 0.0099 | 0.0013 | 0.0235 | 0.0048 | 0.0282 | 0.0055 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0.0004 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0094 | 0.0015 | 0.0031 | 0.001 | 0.0049 | 0.0027 | 0.0095 | 0.0025 | 0.005 | 0.0042 | 0.0027 | 0.0031 | 0.0034 | 0.0019 | 0.0036 | 0.0015 | 0.0013 | 0.0053 |
Endosome | 0.0337 | 0.0013 | 0.0068 | 0.001 | 0.0236 | 0.0069 | 0.032 | 0.0031 | 0.0124 | 0.0405 | 0.0264 | 0.0088 | 0.0126 | 0.0008 | 0.0264 | 0.0026 | 0.0066 | 0.0135 |
Golgi | 0.0068 | 0.0001 | 0.0012 | 0 | 0.0067 | 0.0007 | 0.0086 | 0.0001 | 0.0021 | 0.0039 | 0.0247 | 0.0013 | 0.0116 | 0 | 0.0265 | 0.0066 | 0.02 | 0.0009 |
Lipid Particles | 0.011 | 0 | 0.0022 | 0 | 0.011 | 0.0004 | 0.0103 | 0 | 0.0028 | 0.0082 | 0.0315 | 0.0034 | 0.038 | 0 | 0.0278 | 0.0016 | 0.0509 | 0 |
Mitochondria | 0.0063 | 0.0003 | 0.0124 | 0.0002 | 0.0149 | 0.0043 | 0.012 | 0.0009 | 0.0053 | 0.0014 | 0.0056 | 0.0011 | 0.0287 | 0.0002 | 0.0293 | 0.0015 | 0.0046 | 0.0015 |
None | 0.0264 | 0.0137 | 0.0118 | 0.0664 | 0.0345 | 0.0089 | 0.0067 | 0.0072 | 0.0043 | 0.0043 | 0.0054 | 0.0029 | 0.0023 | 0.0022 | 0.0021 | 0.0026 | 0.0006 | 0.0026 |
Nuclear Periphery | 0.0169 | 0.0104 | 0.0144 | 0.0087 | 0.0193 | 0.0126 | 0.023 | 0.0106 | 0.0137 | 0.0163 | 0.0123 | 0.0171 | 0.0193 | 0.0091 | 0.0185 | 0.0107 | 0.0173 | 0.0219 |
Nucleolus | 0.0008 | 0.0004 | 0.0005 | 0.0002 | 0.003 | 0.0004 | 0.0006 | 0.0003 | 0.0024 | 0.0015 | 0.0006 | 0.0004 | 0.0004 | 0.0001 | 0.0009 | 0.0014 | 0.0002 | 0.0002 |
Nucleus | 0.3296 | 0.4344 | 0.4801 | 0.5956 | 0.2881 | 0.4414 | 0.298 | 0.3737 | 0.359 | 0.4622 | 0.2362 | 0.4203 | 0.1679 | 0.2368 | 0.2087 | 0.2777 | 0.4316 | 0.2665 |
Peroxisomes | 0.005 | 0.0003 | 0.0081 | 0.0002 | 0.0094 | 0.0021 | 0.0046 | 0.0003 | 0.0014 | 0.0086 | 0.0267 | 0.0016 | 0.0132 | 0 | 0.0215 | 0.0014 | 0.0644 | 0.0002 |
Punctate Nuclear | 0.0158 | 0.0038 | 0.0102 | 0.0041 | 0.0224 | 0.0107 | 0.0092 | 0.0051 | 0.0108 | 0.0307 | 0.0426 | 0.0078 | 0.0028 | 0.0016 | 0.0066 | 0.0631 | 0.0034 | 0.0046 |
Vacuole | 0.0025 | 0.001 | 0.0013 | 0.0003 | 0.0029 | 0.0015 | 0.003 | 0.0014 | 0.0027 | 0.0023 | 0.0017 | 0.0016 | 0.0063 | 0.0005 | 0.0086 | 0.0008 | 0.0007 | 0.0055 |
Vacuole Periphery | 0.0009 | 0.0002 | 0.0003 | 0.0001 | 0.0009 | 0.0003 | 0.0015 | 0.0002 | 0.0009 | 0.0007 | 0.0007 | 0.0006 | 0.0031 | 0.0001 | 0.0025 | 0.0003 | 0.0003 | 0.0016 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 67.0989 | 61.3153 | 52.0926 | 75.1968 | 62.5087 | 67.5585 | 76.147 | 77.925 | 85.4675 | 66.537 |
Translational Efficiency | 1.079 | 1.1616 | 1.3931 | 0.944 | 0.9433 | 1.1988 | 0.963 | 0.9871 | 0.8812 | 0.9703 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
16 | 490 | 2125 | 1840 | 1504 | 1925 | 292 | 1418 | 1520 | 2415 | 2417 | 3258 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1209.19 | 995.99 | 1152.37 | 1340.77 | 831.06 | 990.49 | 1204.25 | 1335.03 | 835.04 | 991.61 | 1158.64 | 1338.27 |
Standard Deviation | 166.44 | 135.82 | 146.72 | 215.84 | 133.25 | 133.51 | 180.66 | 209.19 | 139.10 | 134.00 | 152.17 | 212.99 |
Intensity Change Log 2 | — | -0.279838 | -0.069437 | 0.149021 | — | 0.253190 | 0.535110 | 0.683848 | — | -0.038532 | 0.207973 | 0.391224 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002492 | 0.000938 | 0.000400 | 0.003002 | 0.000240 | 0.000123 | 0.000929 | 0.000727 | 0.000264 | 0.000288 | 0.000464 | 0.002012 |
Bud Neck | 0.024067 | 0.004124 | 0.004529 | 0.019304 | 0.001666 | 0.003068 | 0.003477 | 0.016736 | 0.001902 | 0.003282 | 0.004402 | 0.018186 |
Bud Site | 0.101341* | 0.010795 | 0.003352 | 0.033086 | 0.003282 | 0.004233 | 0.005488 | 0.037980 | 0.004314 | 0.005565 | 0.003610 | 0.035216 |
Cell Periphery | 0.000463 | 0.000157 | 0.000104 | 0.000220 | 0.000130 | 0.000049 | 0.000306 | 0.000118 | 0.000134 | 0.000071 | 0.000128 | 0.000176 |
Cytoplasm | 0.247879* | 0.579385* | 0.298892* | 0.094375 | 0.596252* | 0.581961* | 0.261854* | 0.130949* | 0.592585* | 0.581438* | 0.294417* | 0.110293* |
Cytoplasmic Foci | 0.040041 | 0.030637 | 0.002324 | 0.037057 | 0.038395 | 0.014208 | 0.009933 | 0.040007 | 0.038413 | 0.017541 | 0.003244 | 0.038341 |
Eisosomes | 0.000072 | 0.000026 | 0.000013 | 0.000017 | 0.000025 | 0.000011 | 0.000092 | 0.000012 | 0.000025 | 0.000014 | 0.000023 | 0.000015 |
Endoplasmic Reticulum | 0.024585 | 0.004017 | 0.003039 | 0.001497 | 0.002658 | 0.001445 | 0.007869 | 0.000686 | 0.002888 | 0.001967 | 0.003623 | 0.001144 |
Endosome | 0.013872 | 0.002170 | 0.001155 | 0.003981 | 0.001848 | 0.002298 | 0.003772 | 0.009249 | 0.001974 | 0.002272 | 0.001471 | 0.006274 |
Golgi | 0.002229 | 0.001997 | 0.000419 | 0.006349 | 0.000384 | 0.000397 | 0.000178 | 0.013853 | 0.000403 | 0.000722 | 0.000390 | 0.009615 |
Lipid Particles | 0.004314 | 0.000902 | 0.000413 | 0.001268 | 0.001152 | 0.000509 | 0.002719 | 0.001055 | 0.001185 | 0.000588 | 0.000692 | 0.001175 |
Mitochondria | 0.001076 | 0.001357 | 0.001055 | 0.002030 | 0.001257 | 0.001872 | 0.000640 | 0.002691 | 0.001255 | 0.001768 | 0.001005 | 0.002318 |
Mitotic Spindle | 0.012379 | 0.005535 | 0.014932 | 0.560282* | 0.002496 | 0.016112 | 0.016227 | 0.583879* | 0.002600 | 0.013966 | 0.015088 | 0.570552* |
None | 0.001252 | 0.005007 | 0.008585 | 0.005278 | 0.007766 | 0.005338 | 0.011045 | 0.004961 | 0.007698 | 0.005271 | 0.008882 | 0.005140 |
Nuclear Periphery | 0.003429 | 0.000647 | 0.002031 | 0.003416 | 0.000649 | 0.001152 | 0.001889 | 0.001311 | 0.000678 | 0.001049 | 0.002014 | 0.002500 |
Nuclear Periphery Foci | 0.043012 | 0.003335 | 0.001153 | 0.005218 | 0.002312 | 0.000938 | 0.003964 | 0.002756 | 0.002740 | 0.001425 | 0.001492 | 0.004146 |
Nucleolus | 0.004696 | 0.000388 | 0.000811 | 0.004517 | 0.000542 | 0.000382 | 0.000883 | 0.002507 | 0.000586 | 0.000384 | 0.000819 | 0.003642 |
Nucleus | 0.304564* | 0.305906* | 0.631433* | 0.187810* | 0.312111* | 0.335234* | 0.550837* | 0.112495 | 0.312031* | 0.329283* | 0.621696* | 0.155030* |
Peroxisomes | 0.001379 | 0.000885 | 0.000301 | 0.005581 | 0.000430 | 0.000379 | 0.000368 | 0.006493 | 0.000440 | 0.000482 | 0.000309 | 0.005978 |
Vacuole | 0.154979 | 0.041278 | 0.024901 | 0.019220 | 0.026105 | 0.029059 | 0.117310 | 0.027809 | 0.027461 | 0.031538 | 0.036065 | 0.022958 |
Vacuole Periphery | 0.011878 | 0.000514 | 0.000157 | 0.006491 | 0.000302 | 0.001232 | 0.000219 | 0.003725 | 0.000424 | 0.001086 | 0.000165 | 0.005287 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.95 | 3.89 | 2.54 | -0.97 | -2.92 | 3.71 | -9.83 | -6.54 | -9.57 | 5.69 | 0.01 | -4.90 | -5.53 | -5.07 | -2.27 |
Bud Neck | 1.63 | 1.49 | -0.58 | -17.29 | -17.58 | -4.84 | -7.95 | -17.42 | -15.98 | -12.33 | -4.18 | -10.63 | -26.23 | -24.52 | -21.52 |
Bud Site | 1.95 | 2.08 | 1.27 | -10.26 | -17.99 | -1.37 | -3.13 | -15.06 | -14.49 | -11.02 | -1.27 | -0.65 | -21.71 | -21.18 | -22.20 |
Cell Periphery | 3.78 | 4.31 | 3.04 | -4.05 | -10.19 | 11.50 | -7.88 | -2.21 | -13.18 | 6.74 | 8.41 | 0.23 | -6.21 | -16.26 | -7.38 |
Cytoplasm | -5.08 | 0.18 | 2.58 | 37.53 | 25.54 | 2.51 | 31.97 | 60.02* | 60.98* | 4.80 | 2.47 | 45.07 | 67.75 | 77.41* | 25.11 |
Cytoplasmic Foci | 0.99 | 3.87 | 0.64 | -1.02 | -13.65 | 11.27 | 15.68 | 2.56 | -6.10 | -9.57 | 9.76 | 19.12 | 2.14 | -7.64 | -17.61 |
Eisosomes | 3.56 | 4.23 | 4.05 | 2.81 | -2.31 | 9.26 | -10.44 | 2.28 | -9.50 | 11.14 | 7.31 | -1.38 | 2.83 | -7.72 | 5.28 |
Endoplasmic Reticulum | 3.47 | 3.51 | 3.93 | 5.57 | 11.37 | 5.15 | -10.03 | 5.72 | -0.02 | 11.95 | 3.57 | -6.86 | 6.01 | 2.35 | 16.28 |
Endosome | 2.92 | 3.22 | 2.75 | -2.16 | -8.04 | -0.97 | -5.02 | -6.25 | -4.04 | -1.96 | -0.70 | 3.82 | -6.03 | -3.60 | -9.81 |
Golgi | 0.30 | 1.58 | 0.21 | -0.19 | -3.47 | 0.00 | 1.08 | -3.33 | -3.36 | -3.58 | -0.90 | 0.95 | -4.43 | -3.54 | -4.88 |
Lipid Particles | 3.12 | 3.37 | 2.11 | -8.68 | -12.70 | 5.97 | -6.44 | -6.68 | -13.26 | 2.36 | 5.69 | 3.36 | -8.19 | -16.44 | -14.11 |
Mitochondria | -0.32 | -0.91 | -3.54 | -2.21 | -8.98 | -0.96 | 0.66 | -0.99 | 0.16 | -4.48 | -0.80 | -0.12 | -1.78 | -1.36 | -7.64 |
Mitotic Spindle | 0.67 | -0.47 | -33.92* | -47.29* | -46.21* | -5.54 | -3.25 | -43.71* | -41.52* | -33.15* | -5.52 | -7.32 | -64.98 | -61.44* | -61.13* |
None | -4.60 | -8.72 | -9.41 | -5.92 | -1.03 | 2.41 | -0.13 | 1.06 | -2.29 | 0.98 | 2.56 | -0.16 | -0.02 | -5.04 | 0.28 |
Nuclear Periphery | 1.12 | 0.05 | -0.94 | -18.65 | -8.84 | -3.74 | -13.48 | -17.82 | -11.78 | -3.93 | -3.40 | -25.31 | -24.62 | -18.17 | -9.81 |
Nuclear Periphery Foci | 1.62 | 1.73 | 1.31 | -6.60 | -9.61 | 3.66 | -2.38 | -6.33 | -8.74 | -1.83 | 2.81 | 3.19 | -8.40 | -11.15 | -11.54 |
Nucleolus | 1.87 | 1.72 | -4.47 | -13.95 | -13.51 | 0.18 | -1.95 | -10.47 | -10.44 | -9.90 | 0.78 | -0.75 | -17.00 | -17.08 | -16.49 |
Nucleus | -0.42 | -5.15 | 0.73 | 6.60 | 48.94 | -3.24 | -12.56 | 14.07 | 17.69 | 19.74 | -2.74 | -40.91 | 13.24 | 18.00 | 60.45 |
Peroxisomes | 1.56 | 4.26 | -3.54 | -4.95 | -7.97 | -0.01 | 1.09 | -6.18 | -6.27 | -6.56 | -0.63 | 1.82 | -9.26 | -9.18 | -10.29 |
Vacuole | 2.54 | 2.76 | 2.63 | 0.64 | -1.75 | -2.01 | -12.22 | -12.32 | -11.06 | 7.35 | -3.01 | -9.70 | -11.69 | -9.27 | -1.37 |
Vacuole Periphery | 1.18 | 1.22 | 1.08 | -1.60 | -2.37 | -2.26 | 1.44 | -2.31 | 0.64 | -2.71 | -1.90 | 2.51 | -2.22 | -0.25 | -3.25 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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