ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.99 0.93 0.89 0.87 0.73 0.72 0.63 0.68 0.63 0.56 0.99 0.99 1.0 0.93 0.93 0.93 0.95 0.92 0.93 0.93 0.95 0.98
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.21 0.15 0.32 0.27 0.41 0.36 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.07 0.1 0.06 0.1 0.2 0.18 0.09 0.06 0.11 0 0 0 0.07 0.11 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 1 0 0 0 1 0 3 5 5 2 6 1 0 0 0 0 0 0 0 1 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 4 2 2 3 1 6 2 2 1 2 0 14 14 11 0 0 0 0 0 0
Cytoplasm 297 323 165 167 293 203 228 266 110 165 114 64 156 176 160 414 324 311 286 313 175 129 187 293
Endoplasmic Reticulum 2 4 1 1 3 3 4 0 0 1 0 0 1 1 0 10 6 9 0 3 0 1 2 0
Endosome 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 1 0 2 5 0 1 0 0
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 2 0 0 0
Mitochondria 4 1 0 3 4 19 67 54 55 66 74 41 0 0 0 6 4 1 1 4 0 0 1 1
Nucleus 1 1 1 3 5 8 11 14 7 10 7 7 1 4 0 12 1 3 1 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 3 4 1 12 32 15 32 73 31 21 11 13 0 3 0 32 37 33 0 1 1 0 1 0
Unique Cell Count 303 332 166 179 328 234 313 372 174 244 182 114 157 178 160 443 349 335 300 341 189 139 198 299
Labelled Cell Count 309 334 169 187 342 250 348 416 210 273 214 130 159 186 160 488 388 369 300 341 189 139 198 299


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 9.3 8.0 7.9 8.2 6.8 6.3 7.1 6.1 6.4 5.6 6.0 8.6 8.9 9.2 10.7 11.6 11.7 8.2 8.7 9.3
Std Deviation (1e-4) 0.7 2.0 1.1 1.7 1.5 1.4 1.3 1.1 1.4 1.2 1.1 1.2 1.2 1.1 1.2 1.9 2.2 2.0 1.4 1.6 1.4
Intensity Change (Log2) – – – -0.02 0.03 -0.23 -0.34 -0.17 -0.39 -0.31 -0.5 -0.42 0.1 0.15 0.21 0.42 0.54 0.55 0.03 0.13 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.0 -4.1 -4.6 -7.2 -7.5 -8.5 -8.0 -8.6 -9.2 0 -0.5 1.0 -3.0 -3.2 -3.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.8 6.4 5.2 7.9 7.3 9.3 8.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.0 3.8 2.9 4.0 5.9 5.4 3.5 2.8 4.1 0 0 0 3.2 4.1 3.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0217 4.9731 4.3577 3.8165 4.071 4.4138 2.9327 4.273 3.581 2.9403 3.3136 3.6164 3.1166 3.6965 3.0886 3.3183 3.1163 3.3635
Actin 0.0327 0.0011 0.0171 0.0153 0.002 0.003 0.0084 0.0005 0.0016 0.0004 0.0095 0.0005 0.013 0.0015 0.0068 0.0048 0.1098 0.0013
Bud 0.002 0.0007 0.0011 0.0005 0.0004 0.0003 0.0007 0.0042 0.0002 0.0001 0.0047 0.0026 0.0012 0.0002 0.0003 0.0194 0.0152 0.0005
Bud Neck 0.0131 0.0004 0.0035 0.0014 0.0207 0.0036 0.0021 0.0007 0.0004 0.0012 0.0122 0.0013 0.0043 0.0002 0.0006 0.0013 0.0009 0.0032
Bud Periphery 0.0036 0.0009 0.0009 0.0005 0.0003 0.0003 0.0005 0.0006 0.0001 0.0001 0.0051 0.0005 0.001 0.0002 0.0003 0.0123 0.0124 0.0003
Bud Site 0.0129 0.0025 0.0025 0.0009 0.002 0.0002 0.0016 0.0067 0.0006 0.0005 0.0035 0.0005 0.0145 0.0031 0.0014 0.0176 0.0041 0.0001
Cell Periphery 0.0006 0.0001 0.0001 0.0001 0.0001 0 0.0001 0.0001 0 0.0001 0.0012 0 0.0005 0.0001 0.0001 0.0005 0.0003 0
Cytoplasm 0.7126 0.9083 0.847 0.8184 0.7992 0.8973 0.8633 0.9416 0.9126 0.725 0.7505 0.9158 0.6228 0.9257 0.8687 0.7895 0.6993 0.9156
Cytoplasmic Foci 0.0281 0.0088 0.0162 0.0194 0.0438 0.0125 0.0137 0.0078 0.007 0.0128 0.0223 0.0096 0.0468 0.018 0.0291 0.0201 0.0124 0.012
Eisosomes 0.0005 0 0 0.0001 0 0 0.0003 0 0 0 0.0002 0 0.0003 0 0.0002 0 0.0007 0
Endoplasmic Reticulum 0.0097 0.0019 0.0027 0.0028 0.0019 0.0021 0.0046 0.0018 0.0033 0.0077 0.0052 0.0023 0.0117 0.0021 0.0019 0.0044 0.0084 0.0044
Endosome 0.0327 0.0113 0.0108 0.0231 0.0267 0.0107 0.0061 0.0022 0.0035 0.018 0.0257 0.005 0.0585 0.0061 0.02 0.0437 0.0517 0.0101
Golgi 0.0149 0.0007 0.0054 0.0124 0.0043 0.004 0.0016 0.0002 0.001 0.0004 0.0055 0.0002 0.0255 0.0013 0.0081 0.0061 0.0116 0.0045
Lipid Particles 0.0114 0.0012 0.003 0.0022 0.001 0.0013 0.0028 0 0.0024 0.0004 0.0211 0.0001 0.0269 0.0022 0.0048 0.0009 0.0045 0.0003
Mitochondria 0.0147 0.0028 0.0072 0.0371 0.0009 0.0037 0.0014 0.0002 0.0009 0.0005 0.016 0.0004 0.011 0.0007 0.0135 0.0275 0.0147 0.0012
None 0.0648 0.04 0.0521 0.0412 0.0668 0.0383 0.063 0.0197 0.0395 0.1311 0.0641 0.0249 0.0792 0.0274 0.0311 0.0075 0.0066 0.0273
Nuclear Periphery 0.0056 0.0019 0.0022 0.0017 0.001 0.0017 0.003 0.0012 0.0022 0.0076 0.0153 0.0024 0.0113 0.0011 0.0012 0.0058 0.0201 0.0028
Nucleolus 0.0006 0.0004 0.0004 0.0002 0.0001 0.0001 0.0003 0.0001 0.0001 0.0025 0.001 0.0002 0.0018 0.0001 0.0001 0.0004 0.0004 0
Nucleus 0.0124 0.0127 0.0159 0.0156 0.0126 0.0151 0.0155 0.0096 0.0159 0.0679 0.0186 0.0269 0.0276 0.0035 0.0031 0.01 0.0091 0.0126
Peroxisomes 0.0186 0.0005 0.005 0.0025 0.0098 0.0019 0.0047 0.0002 0.0055 0.0001 0.0058 0.0002 0.0141 0.0044 0.0065 0.0026 0.0026 0.0016
Punctate Nuclear 0.0028 0.0016 0.0053 0.0024 0.0031 0.0026 0.0044 0.0015 0.0015 0.0174 0.0058 0.0056 0.0175 0.0007 0.0011 0.0024 0.0033 0.0008
Vacuole 0.0044 0.0017 0.0013 0.0016 0.0026 0.0009 0.0013 0.001 0.0014 0.0058 0.0048 0.0009 0.0083 0.0011 0.0009 0.0193 0.0077 0.0011
Vacuole Periphery 0.0015 0.0005 0.0003 0.0005 0.0005 0.0002 0.0003 0.0001 0.0002 0.0006 0.0019 0.0002 0.0022 0.0002 0.0003 0.0037 0.004 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.5144 27.6296 32.8038 30.9611 24.7118 32.9499 32.3595 30.7966 35.924 24.0456
Translational Efficiency 1.2446 0.8685 0.7678 1.2658 1.0378 0.7595 0.8521 1.1028 0.9247 1.0112

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1767 1328 2145 1547 1741 1988 74 1228 3508 3316 2219 2775

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 837.35 933.60 1014.77 1097.56 835.88 928.66 1370.50 1101.88 836.62 930.64 1026.63 1099.47
Standard Deviation 120.16 152.90 136.97 166.96 114.50 119.57 243.84 160.29 117.39 133.94 155.56 164.05
Intensity Change Log 2 — 0.156974 0.277250 0.390397 — 0.151855 0.713335 0.398599 — 0.154419 0.511517 0.394500

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000149 0.000231 0.000093 0.001995 0.000105 0.000355 0.001683 0.002712 0.000127 0.000305 0.000146 0.002312
Bud Neck 0.004093 0.002421 0.001000 0.005756 0.001869 0.004464 0.022516 0.005462 0.002989 0.003646 0.001718 0.005626
Bud Site 0.005118 0.006976 0.001491 0.036789 0.004909 0.014451 0.096799 0.045595 0.005015 0.011457 0.004670 0.040686
Cell Periphery 0.000083 0.000091 0.000055 0.000120 0.000078 0.000061 0.000333 0.000104 0.000081 0.000073 0.000064 0.000113
Cytoplasm 0.695022* 0.759911* 0.630293* 0.583720* 0.737222* 0.725124* 0.231920* 0.559242* 0.715966* 0.739056* 0.617008* 0.572888*
Cytoplasmic Foci 0.112006 0.072595 0.005072 0.013950 0.079284 0.064531 0.022743 0.033426 0.095766 0.067761 0.005661 0.022569
Eisosomes 0.000036 0.000020 0.000009 0.000017 0.000021 0.000020 0.000051 0.000022 0.000028 0.000020 0.000010 0.000019
Endoplasmic Reticulum 0.001276 0.001190 0.001111 0.001365 0.001377 0.000692 0.003935 0.001055 0.001326 0.000891 0.001205 0.001228
Endosome 0.005118 0.004864 0.000629 0.003948 0.003216 0.005997 0.016711 0.015966 0.004174 0.005543 0.001165 0.009266
Golgi 0.001690 0.001753 0.000045 0.003857 0.000677 0.002737 0.001052 0.021223 0.001187 0.002343 0.000079 0.011542
Lipid Particles 0.001931 0.001073 0.000338 0.000943 0.001088 0.000992 0.005193 0.000823 0.001513 0.001025 0.000500 0.000890
Mitochondria 0.001023 0.000559 0.000168 0.002250 0.001020 0.001458 0.000302 0.003292 0.001022 0.001098 0.000172 0.002711
Mitotic Spindle 0.001612 0.003192 0.002140 0.078140 0.001849 0.016043 0.029946 0.106446* 0.001729 0.010896 0.003067 0.090666
None 0.003696 0.007627 0.008894 0.006994 0.004702 0.005605 0.000850 0.006244 0.004195 0.006415 0.008625 0.006662
Nuclear Periphery 0.000216 0.000240 0.000636 0.000833 0.000274 0.000250 0.001081 0.000547 0.000245 0.000246 0.000650 0.000706
Nuclear Periphery Foci 0.001604 0.003056 0.001226 0.002245 0.001699 0.000957 0.007822 0.002040 0.001651 0.001798 0.001446 0.002154
Nucleolus 0.000654 0.000588 0.000175 0.000266 0.000330 0.000848 0.002347 0.000865 0.000493 0.000744 0.000247 0.000531
Nucleus 0.104574 0.084974 0.318390* 0.160090* 0.119627 0.085586 0.141789* 0.073272 0.112045 0.085341 0.312501* 0.121671
Peroxisomes 0.001030 0.001392 0.000050 0.002370 0.000356 0.001910 0.002248 0.002683 0.000696 0.001703 0.000123 0.002508
Vacuole 0.058453 0.046501 0.028115 0.089685 0.039669 0.066601 0.408120* 0.105330 0.049131 0.058551 0.040788 0.096609
Vacuole Periphery 0.000616 0.000744 0.000071 0.004667 0.000627 0.001317 0.002559 0.013651 0.000621 0.001088 0.000154 0.008643

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.13 1.19 -3.99 -3.52 -4.11 -2.83 -5.73 -2.99 -1.87 1.84 -3.40 -2.37 -4.99 -3.68 -4.72
Bud Neck 2.93 6.10 0.63 -2.68 -6.88 -4.78 -3.54 -3.25 1.44 3.21 -1.58 3.35 -1.69 0.06 -4.96
Bud Site -2.10 5.71 -4.82 -3.47 -6.91 -4.97 -3.26 -4.41 -0.28 2.57 -5.53 1.96 -6.48 -1.74 -7.44
Cell Periphery -1.25 4.31 -3.45 -1.82 -7.68 2.06 -4.02 0.14 -4.05 4.10 1.58 1.94 -1.97 -4.96 -4.91
Cytoplasm -7.28 14.05 22.72 28.45 10.31 2.35 20.20 23.54 20.93 -11.44 -3.12 21.41 33.09 34.44 9.22
Cytoplasmic Foci 8.65 31.91 30.51 19.98 -5.56 3.80 14.91 21.53 19.08 0.55 9.50 40.64 37.14 28.33 -8.07
Eisosomes 6.64 11.22 6.28 -1.14 -8.74 0.21 -5.26 -0.39 -0.61 5.17 5.65 11.22 4.97 -1.24 -8.70
Endoplasmic Reticulum 0.29 -1.02 -0.14 -0.64 1.46 3.10 -4.65 2.92 -0.61 6.47 2.76 -1.39 1.79 -1.85 4.79
Endosome 0.38 9.22 7.33 7.89 -2.82 -4.64 -3.82 -0.59 3.12 3.59 -3.06 9.71 4.46 7.51 -4.19
Golgi -0.01 3.62 1.58 1.38 -2.06 -3.07 -0.41 -2.71 -0.85 -2.57 -2.15 4.40 -1.50 0.29 -3.82
Lipid Particles 2.93 5.57 1.95 -2.47 -14.12 0.61 -2.87 -1.69 -1.68 2.77 2.72 5.46 1.11 -2.68 -8.04
Mitochondria 1.37 2.69 0.12 -1.87 -4.69 -0.64 1.14 0.30 1.87 -2.72 -0.12 2.56 0.30 0.71 -6.04
Mitotic Spindle -1.41 -0.68 -8.77 -7.74 -8.44 -6.06 -2.44 -9.31 -5.39 0.06 -6.20 -1.74 -12.76 -8.18 -11.45
None -3.11 -7.28 -5.92 0.41 1.77 -0.67 5.40 -3.42 -1.94 -10.23 -2.58 -6.31 -6.20 -1.21 1.13
Nuclear Periphery -0.80 -10.81 -20.16 -21.63 -10.72 0.48 -6.09 -18.85 -20.10 1.88 -0.42 -12.94 -27.51 -29.52 -10.94
Nuclear Periphery Foci -4.06 -0.44 -2.16 2.87 -1.96 4.68 -3.33 -0.57 -5.98 3.29 -0.98 -1.52 -2.09 -0.77 -0.06
Nucleolus 0.08 1.90 1.30 2.03 -2.23 -1.85 -2.30 -0.69 1.52 2.11 -1.27 2.08 0.95 2.20 -2.35
Nucleus 4.33 -42.21 -19.94 -22.78 19.89 8.53 -0.08 -1.85 -9.50 -0.40 9.13 -42.07 -16.51 -24.10 27.84
Peroxisomes -1.03 6.38 1.83 2.18 -2.85 -4.91 -2.14 -1.78 3.71 1.80 -4.25 6.70 0.58 4.28 -3.76
Vacuole 2.44 3.30 -23.43 -24.50 -25.19 -9.55 -13.01 -28.20 -23.12 6.68 -5.52 -3.75 -36.52 -32.70 -29.97
Vacuole Periphery -0.50 3.77 -0.70 -0.43 -2.08 -1.61 -1.29 -2.54 -1.52 -1.31 -1.73 3.84 -2.42 -1.21 -3.94
ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YDL144C

YDL144C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ydl144c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available