Standard name | |
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Human Ortholog | |
Description | Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 7 | 5 | 5 | 0 | 2 | 2 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 14 | 10 | 6 | 46 | 15 | 18 | 17 | 31 | 55 |
Bud | 11 | 4 | 4 | 1 | 5 | 4 | 7 | 8 | 1 | 5 | 5 | 4 | 0 | 0 | 0 | 7 | 4 | 2 | 4 | 4 | 1 | 2 | 5 | 9 |
Bud Neck | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 11 | 11 | 6 | 9 | 11 | 17 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 4 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 13 | 11 | 19 | 17 | 9 | 10 | 2 | 3 | 1 | 1 | 4 | 11 |
Cytoplasm | 155 | 89 | 64 | 34 | 88 | 48 | 73 | 106 | 59 | 73 | 32 | 42 | 237 | 290 | 302 | 253 | 155 | 101 | 114 | 79 | 46 | 97 | 156 | 176 |
Endoplasmic Reticulum | 4 | 9 | 2 | 1 | 2 | 0 | 2 | 1 | 1 | 1 | 0 | 3 | 1 | 0 | 2 | 21 | 6 | 6 | 1 | 1 | 2 | 3 | 7 | 7 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 17 | 3 | 2 | 7 | 1 | 6 | 5 | 4 | 7 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 |
Mitochondria | 36 | 0 | 2 | 8 | 5 | 22 | 55 | 55 | 53 | 61 | 47 | 83 | 0 | 0 | 0 | 12 | 0 | 2 | 10 | 4 | 7 | 12 | 10 | 11 |
Nucleus | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 2 | 1 | 0 | 0 | 4 | 4 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 6 | 3 | 3 | 2 | 0 | 3 | 0 | 0 | 2 |
Vac/Vac Membrane | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 10 | 4 | 4 | 1 | 3 | 0 | 1 | 3 | 7 |
Unique Cell Count | 188 | 99 | 72 | 40 | 96 | 65 | 114 | 162 | 97 | 119 | 69 | 111 | 240 | 293 | 304 | 307 | 175 | 117 | 206 | 128 | 102 | 156 | 240 | 312 |
Labelled Cell Count | 223 | 112 | 81 | 47 | 105 | 77 | 143 | 183 | 116 | 151 | 88 | 139 | 254 | 304 | 323 | 368 | 197 | 138 | 206 | 128 | 102 | 156 | 240 | 312 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.5 | 5.6 | 4.6 | 4.2 | 4.9 | 4.6 | 3.3 | 3.7 | 3.7 | 3.6 | 3.3 | 3.0 | 6.6 | 7.2 | 7.9 | 6.7 | 7.7 | 7.7 | 4.9 | 5.5 | 6.4 |
Std Deviation (1e-4) | 0.4 | 0.6 | 1.1 | 1.0 | 2.0 | 2.5 | 1.0 | 1.3 | 1.6 | 2.0 | 0.9 | 0.8 | 1.1 | 1.1 | 1.5 | 1.6 | 1.6 | 1.9 | 1.2 | 1.2 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.1 | 0.02 | -0.45 | -0.31 | -0.31 | -0.35 | -0.48 | -0.6 | 0.53 | 0.66 | 0.8 | 0.55 | 0.75 | 0.75 | 0.11 | 0.28 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | 0.6 | -2.3 | -3.7 | -3.7 | -4.1 | -4.1 | -5.4 | -6.8 | 4.0 | 4.5 | 5.0 | -1.3 | -0.1 | -0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5639 | 2.6717 | 2.5412 | 2.2237 | 2.6625 | 2.8525 | 2.5018 | 2.6734 | 2.8231 | 3.2577 | 2.7889 | 2.9888 | 3.4521 | 3.9243 | 3.7916 | 4.4989 | 3.7276 | 3.8383 |
Actin | 0.1151 | 0.0632 | 0.1553 | 0.0351 | 0.0723 | 0.0426 | 0.0558 | 0.0393 | 0.085 | 0.0141 | 0.0378 | 0.0392 | 0.0529 | 0.0286 | 0.1087 | 0.0396 | 0.0827 | 0.0551 |
Bud | 0.0039 | 0.0024 | 0.0175 | 0.0059 | 0.0017 | 0.0007 | 0.0017 | 0.0013 | 0.0134 | 0.0058 | 0.0024 | 0.0009 | 0.0026 | 0.0029 | 0.0074 | 0.013 | 0.002 | 0.0012 |
Bud Neck | 0.0163 | 0.0035 | 0.0067 | 0.0556 | 0.0199 | 0.1511 | 0.0045 | 0.0018 | 0.0078 | 0.0586 | 0.005 | 0.062 | 0.0026 | 0.0061 | 0.024 | 0.002 | 0.0049 | 0.0759 |
Bud Periphery | 0.0079 | 0.0032 | 0.0214 | 0.0377 | 0.0034 | 0.0012 | 0.0034 | 0.0016 | 0.0423 | 0.0166 | 0.0024 | 0.0022 | 0.0108 | 0.0034 | 0.0148 | 0.0509 | 0.0045 | 0.0038 |
Bud Site | 0.0168 | 0.0556 | 0.031 | 0.0124 | 0.0102 | 0.0018 | 0.0294 | 0.0442 | 0.0228 | 0.0152 | 0.0032 | 0.0008 | 0.0279 | 0.1184 | 0.0338 | 0.0039 | 0.0095 | 0.0014 |
Cell Periphery | 0.0019 | 0.0037 | 0.0019 | 0.0028 | 0.0013 | 0.0009 | 0.0045 | 0.0021 | 0.0021 | 0.0018 | 0.0006 | 0.0015 | 0.0064 | 0.0034 | 0.0033 | 0.0046 | 0.001 | 0.0024 |
Cytoplasm | 0.5776 | 0.681 | 0.5779 | 0.7584 | 0.6546 | 0.6504 | 0.626 | 0.6446 | 0.5277 | 0.6698 | 0.5388 | 0.6391 | 0.6086 | 0.5693 | 0.5485 | 0.548 | 0.5477 | 0.6609 |
Cytoplasmic Foci | 0.0462 | 0.0279 | 0.0235 | 0.0185 | 0.0429 | 0.0304 | 0.0311 | 0.0117 | 0.0194 | 0.0089 | 0.0635 | 0.0214 | 0.0203 | 0.0183 | 0.0228 | 0.0364 | 0.0314 | 0.0231 |
Eisosomes | 0.0008 | 0.0005 | 0.0004 | 0.0003 | 0.0009 | 0.0001 | 0.0021 | 0.0004 | 0.0005 | 0.0003 | 0.0008 | 0.0004 | 0.0008 | 0.0004 | 0.0007 | 0.0009 | 0.0008 | 0.0007 |
Endoplasmic Reticulum | 0.0083 | 0.0046 | 0.0045 | 0.0051 | 0.0107 | 0.0038 | 0.0065 | 0.0026 | 0.0027 | 0.0044 | 0.0024 | 0.0029 | 0.008 | 0.0047 | 0.0072 | 0.0065 | 0.0069 | 0.0032 |
Endosome | 0.0304 | 0.0079 | 0.0176 | 0.0166 | 0.0242 | 0.0099 | 0.0134 | 0.0079 | 0.0061 | 0.0116 | 0.0171 | 0.0061 | 0.0119 | 0.0054 | 0.0065 | 0.0076 | 0.0254 | 0.0062 |
Golgi | 0.013 | 0.0048 | 0.0075 | 0.0032 | 0.0181 | 0.0065 | 0.0077 | 0.0016 | 0.0062 | 0.0056 | 0.0081 | 0.0049 | 0.0074 | 0.002 | 0.0057 | 0.003 | 0.013 | 0.0021 |
Lipid Particles | 0.0113 | 0.001 | 0.0033 | 0.0004 | 0.0174 | 0.0008 | 0.0026 | 0.0002 | 0.0009 | 0.0011 | 0.0125 | 0.0014 | 0.0043 | 0.0009 | 0.0082 | 0.0012 | 0.0421 | 0.0018 |
Mitochondria | 0.0078 | 0.0013 | 0.0096 | 0.0023 | 0.0172 | 0.0013 | 0.002 | 0.0004 | 0.0015 | 0.0544 | 0.0278 | 0.0039 | 0.0022 | 0.0004 | 0.0023 | 0.0027 | 0.0506 | 0.0027 |
None | 0.1076 | 0.1311 | 0.1027 | 0.0364 | 0.0686 | 0.0906 | 0.1972 | 0.2368 | 0.2537 | 0.119 | 0.2595 | 0.206 | 0.2206 | 0.227 | 0.1874 | 0.2657 | 0.1375 | 0.1476 |
Nuclear Periphery | 0.0087 | 0.0007 | 0.0033 | 0.001 | 0.0018 | 0.0006 | 0.0015 | 0.0003 | 0.0006 | 0.0006 | 0.0011 | 0.0012 | 0.0011 | 0.0009 | 0.0029 | 0.0036 | 0.004 | 0.0012 |
Nucleolus | 0.0008 | 0.0002 | 0.0003 | 0.0005 | 0.0016 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0.0005 | 0.0007 | 0.0001 | 0.0004 | 0.0002 | 0.0004 | 0.0003 | 0.001 | 0.001 |
Nucleus | 0.0027 | 0.001 | 0.0015 | 0.0018 | 0.0017 | 0.0015 | 0.0024 | 0.0007 | 0.0012 | 0.001 | 0.0012 | 0.0006 | 0.0014 | 0.0017 | 0.0032 | 0.0013 | 0.0027 | 0.0011 |
Peroxisomes | 0.0099 | 0.0036 | 0.0078 | 0.0018 | 0.0215 | 0.0027 | 0.0027 | 0.0006 | 0.0026 | 0.0014 | 0.0104 | 0.0034 | 0.004 | 0.0017 | 0.0088 | 0.0023 | 0.0252 | 0.0057 |
Punctate Nuclear | 0.0092 | 0.0008 | 0.0034 | 0.0006 | 0.0058 | 0.0011 | 0.0035 | 0.0003 | 0.0016 | 0.0003 | 0.0015 | 0.0009 | 0.0012 | 0.0011 | 0.0012 | 0.0042 | 0.0039 | 0.0019 |
Vacuole | 0.0025 | 0.0019 | 0.0021 | 0.003 | 0.0033 | 0.0014 | 0.0013 | 0.0014 | 0.0013 | 0.0076 | 0.0023 | 0.0009 | 0.0042 | 0.0029 | 0.0019 | 0.0016 | 0.0023 | 0.001 |
Vacuole Periphery | 0.0014 | 0.0003 | 0.0008 | 0.0006 | 0.0008 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0013 | 0.0011 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.0007 | 0.0009 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.8494 | 6.0145 | 4.9589 | 9.134 | 7.2694 | 15.6902 | 9.1219 | 13.9095 | 12.6168 | 12.7182 |
Translational Efficiency | 0.3365 | 0.515 | 0.6749 | 0.4858 | 0.3553 | 0.3648 | 0.4329 | 0.3701 | 0.3288 | 0.3504 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2206 | 1256 | 991 | 928 | 746 | 1876 | 228 | 2979 | 2952 | 3132 | 1219 | 3907 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 750.12 | 706.25 | 943.43 | 876.53 | 851.92 | 779.60 | 1059.37 | 935.51 | 775.85 | 750.19 | 965.12 | 921.50 |
Standard Deviation | 79.89 | 114.20 | 160.62 | 111.64 | 118.20 | 103.09 | 115.50 | 115.66 | 101.28 | 113.53 | 159.73 | 117.43 |
Intensity Change Log 2 | — | -0.086942 | 0.330794 | 0.224682 | — | -0.127984 | 0.314417 | 0.135035 | — | -0.108622 | 0.322108 | 0.177705 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.024618 | 0.011573 | 0.048570 | 0.054386 | 0.029147 | 0.006628 | 0.052694 | 0.036716 | 0.025763 | 0.008611 | 0.049341 | 0.040913 |
Bud Neck | 0.083143 | 0.035492 | 0.017363 | 0.015383 | 0.027117 | 0.053067 | 0.016977 | 0.046933 | 0.068985 | 0.046019 | 0.017290 | 0.039439 |
Bud Site | 0.077099 | 0.060375 | 0.227496* | 0.132747* | 0.104999* | 0.064011 | 0.164835* | 0.103266* | 0.084150 | 0.062553 | 0.215776* | 0.110268* |
Cell Periphery | 0.001674 | 0.000417 | 0.000810 | 0.001192 | 0.001259 | 0.000596 | 0.001411 | 0.000787 | 0.001569 | 0.000524 | 0.000923 | 0.000883 |
Cytoplasm | 0.423068* | 0.322193* | 0.376678* | 0.360452* | 0.432250* | 0.388897* | 0.260669* | 0.543490* | 0.425388* | 0.362147* | 0.354980* | 0.500015* |
Cytoplasmic Foci | 0.197488* | 0.320853* | 0.153200 | 0.086566 | 0.265998* | 0.271368* | 0.127267 | 0.026076 | 0.214801* | 0.291213* | 0.148349 | 0.040443 |
Eisosomes | 0.008437 | 0.001185 | 0.000440 | 0.001001 | 0.001797 | 0.001260 | 0.000717 | 0.000744 | 0.006759 | 0.001230 | 0.000492 | 0.000805 |
Endoplasmic Reticulum | 0.001882 | 0.002245 | 0.003251 | 0.003220 | 0.003771 | 0.000782 | 0.003552 | 0.000644 | 0.002359 | 0.001369 | 0.003307 | 0.001256 |
Endosome | 0.016793 | 0.056645 | 0.016512 | 0.007151 | 0.019243 | 0.031017 | 0.013567 | 0.004311 | 0.017412 | 0.041294 | 0.015961 | 0.004986 |
Golgi | 0.019743 | 0.026536 | 0.006942 | 0.003964 | 0.021742 | 0.023823 | 0.001177 | 0.000866 | 0.020248 | 0.024911 | 0.005864 | 0.001602 |
Lipid Particles | 0.009220 | 0.017404 | 0.004546 | 0.003282 | 0.006926 | 0.005448 | 0.005892 | 0.001595 | 0.008640 | 0.010242 | 0.004798 | 0.001995 |
Mitochondria | 0.009511 | 0.010222 | 0.000467 | 0.001876 | 0.004746 | 0.014062 | 0.000115 | 0.003597 | 0.008307 | 0.012522 | 0.000401 | 0.003188 |
Mitotic Spindle | 0.001000 | 0.002835 | 0.004524 | 0.006100 | 0.000531 | 0.007322 | 0.000864 | 0.014239 | 0.000881 | 0.005523 | 0.003839 | 0.012306 |
None | 0.001234 | 0.003562 | 0.000609 | 0.000871 | 0.001137 | 0.001019 | 0.000248 | 0.001057 | 0.001209 | 0.002039 | 0.000542 | 0.001013 |
Nuclear Periphery | 0.000058 | 0.000342 | 0.000090 | 0.000226 | 0.000051 | 0.000077 | 0.000223 | 0.000215 | 0.000057 | 0.000183 | 0.000114 | 0.000218 |
Nuclear Periphery Foci | 0.000180 | 0.000745 | 0.003145 | 0.002336 | 0.000818 | 0.000198 | 0.005213 | 0.000139 | 0.000341 | 0.000418 | 0.003532 | 0.000661 |
Nucleolus | 0.000301 | 0.000956 | 0.000124 | 0.000099 | 0.000202 | 0.000507 | 0.000115 | 0.000228 | 0.000276 | 0.000687 | 0.000122 | 0.000197 |
Nucleus | 0.036727 | 0.018804 | 0.017917 | 0.050561 | 0.017195 | 0.021956 | 0.029743 | 0.057704 | 0.031791 | 0.020692 | 0.020129 | 0.056007 |
Peroxisomes | 0.033185 | 0.034001 | 0.024647 | 0.030733 | 0.021728 | 0.031534 | 0.028004 | 0.023896 | 0.030290 | 0.032523 | 0.025274 | 0.025520 |
Vacuole | 0.053600 | 0.070094 | 0.092125 | 0.236791* | 0.037838 | 0.074042 | 0.286486* | 0.132750 | 0.049617 | 0.072459 | 0.128478 | 0.157462 |
Vacuole Periphery | 0.001039 | 0.003522 | 0.000547 | 0.001063 | 0.001505 | 0.002385 | 0.000232 | 0.000748 | 0.001157 | 0.002841 | 0.000488 | 0.000823 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 5.99 | -4.87 | -2.93 | -6.46 | 1.68 | 6.69 | -1.38 | 0.69 | -10.59 | 1.77 | 10.54 | -4.89 | -1.38 | -11.67 | 4.04 |
Bud Neck | 12.88 | 20.38 | 21.98 | 10.01 | 2.55 | -7.03 | 3.26 | -3.04 | 5.61 | -6.87 | 7.85 | 19.87 | 13.79 | 7.13 | -9.26 |
Bud Site | 3.99 | -15.28 | -2.90 | -5.51 | 11.54 | 5.85 | -1.74 | 3.90 | -3.43 | 3.78 | 6.41 | -14.39 | 0.70 | -5.89 | 14.84 |
Cell Periphery | 7.24 | 5.02 | 4.46 | -4.82 | -0.80 | 4.11 | 0.40 | 4.33 | -0.02 | 1.88 | 7.59 | 4.85 | 6.74 | -2.53 | 2.10 |
Cytoplasm | 11.25 | 3.68 | 7.92 | -1.71 | 3.33 | 3.67 | 9.12 | -8.03 | -16.45 | -15.67 | 9.55 | 6.84 | -8.28 | -17.51 | -12.57 |
Cytoplasmic Foci | -16.78 | 7.29 | 25.51 | 35.59 | 12.54 | -0.54 | 13.71 | 31.96 | 48.48 | 10.56 | -14.61 | 12.40 | 48.42 | 62.31 | 21.89 |
Eisosomes | 14.61 | 16.89 | 16.10 | 2.71 | -3.10 | 2.35 | 5.28 | 5.64 | 5.50 | 0.39 | 14.91 | 17.36 | 16.82 | 5.94 | -1.98 |
Endoplasmic Reticulum | -0.75 | -3.31 | -3.60 | -1.99 | 0.67 | 4.05 | 0.33 | 4.22 | 1.03 | 6.22 | 3.10 | -2.52 | 3.94 | 0.67 | 6.94 |
Endosome | -13.19 | 0.78 | 10.25 | 17.55 | 6.22 | -5.18 | 3.26 | 8.43 | 20.05 | 6.12 | -14.48 | 1.76 | 15.01 | 25.82 | 8.30 |
Golgi | -3.68 | 11.31 | 15.78 | 14.06 | 3.44 | -1.01 | 12.71 | 12.93 | 18.68 | 1.42 | -3.67 | 14.66 | 24.66 | 24.14 | 5.70 |
Lipid Particles | -5.40 | 7.67 | 11.87 | 9.62 | 3.28 | 1.66 | 0.83 | 6.30 | 9.53 | 2.28 | -2.24 | 6.55 | 16.09 | 12.60 | 5.93 |
Mitochondria | -0.44 | 12.37 | 8.81 | 6.74 | -1.26 | -5.33 | 3.31 | 1.33 | 10.21 | -8.66 | -4.05 | 12.15 | 8.31 | 11.98 | -7.27 |
Mitotic Spindle | -2.09 | -2.97 | -3.21 | -1.49 | -0.51 | -5.46 | -1.36 | -8.53 | -2.38 | -7.82 | -5.31 | -3.15 | -8.44 | -3.43 | -4.94 |
None | -2.36 | 2.69 | 1.33 | 2.71 | -0.74 | 0.23 | 2.79 | -0.02 | -0.31 | -6.72 | -1.81 | 3.33 | 0.88 | 2.17 | -2.98 |
Nuclear Periphery | -1.94 | -4.67 | -6.70 | -0.26 | -5.61 | -2.26 | -7.56 | -17.71 | -15.31 | -2.44 | -2.15 | -8.20 | -17.62 | -3.42 | -12.22 |
Nuclear Periphery Foci | -4.37 | -9.18 | -5.48 | -3.31 | 2.78 | 5.64 | -4.83 | 6.11 | 0.79 | 5.57 | -1.75 | -10.22 | -3.35 | -1.67 | 9.09 |
Nucleolus | -4.96 | 4.21 | 4.72 | 6.42 | 0.75 | -1.76 | 3.06 | -2.43 | 1.29 | -7.69 | -3.64 | 4.74 | 0.98 | 3.99 | -5.82 |
Nucleus | 8.95 | 8.54 | -7.20 | -12.37 | -12.05 | -3.27 | -4.30 | -25.27 | -23.33 | -8.76 | 8.00 | 4.96 | -19.52 | -26.73 | -22.72 |
Peroxisomes | -0.31 | 4.37 | 4.65 | 4.69 | 0.94 | -4.31 | -0.09 | 1.50 | 6.31 | 0.82 | -1.24 | 3.72 | 6.71 | 8.22 | 1.78 |
Vacuole | -5.12 | -11.63 | -30.12 | -27.43 | -21.23 | -10.27 | -16.18 | -32.56 | -24.22 | 8.12 | -9.64 | -17.70 | -42.20 | -34.92 | -9.41 |
Vacuole Periphery | -7.24 | 3.72 | 1.69 | 7.04 | -0.48 | -3.03 | 6.61 | 4.34 | 7.72 | -3.07 | -8.47 | 5.64 | 5.00 | 10.81 | -0.78 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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