Standard name
Human Ortholog
Description Signal transducing MAP kinase kinase; involved in pheromone response where it phosphorylates Fus3p; involved in the pseudohyphal/invasive growth pathway where it phosphorylates of Kss1p; phosphorylated by Ste11p; degraded by ubiquitin pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.06 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.06 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 1.0 0.97 0.98 0.95 0.94 0.87 0.73 0.75 0.66 0.6 0.5 0.51 0.98 1.0 0.99 0.96 0.9 0.92 0.97 0.92 0.92 0.67 0.8 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.07 0.27 0.34 0.27 0.47 0.55 0.61 0.65 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 0 0 1 0 0 1 1 1 2 0 0 0 0 0 1 0 2 1 13 14 6
Bud 1 3 1 0 3 2 9 5 2 1 8 8 0 0 1 0 0 1 0 2 1 4 4 7
Bud Neck 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 3 2 7 7 4
Bud Site 0 0 1 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 13 7 12 3 10 6 8 13 17 13 13 2 5 7 3 11 14 0 0 3 0 1 0
Cytoplasm 238 383 327 202 220 309 316 292 193 220 159 180 204 262 322 359 253 249 234 376 300 80 184 160
Endoplasmic Reticulum 0 6 2 2 0 7 3 13 11 5 11 6 1 1 0 15 9 6 0 3 2 0 2 2
Endosome 0 0 0 0 4 1 5 10 0 2 1 1 0 0 0 4 1 3 1 5 2 3 1 0
Golgi 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1 1 3 1 0 1 0 0 0 1
Mitochondria 4 3 2 6 16 94 148 103 139 202 197 229 1 0 0 11 5 4 0 2 2 4 5 2
Nucleus 0 1 2 0 1 1 0 4 3 3 3 0 0 0 1 8 5 11 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 2 2 2 4 2 2 0 0 1 0 1 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 3 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 1 0 0 0 1 1 1 0 1
Vac/Vac Membrane 0 0 0 5 4 2 14 6 7 9 14 13 2 0 0 10 19 6 0 4 1 2 2 5
Unique Cell Count 239 395 332 212 234 354 432 388 294 366 321 350 208 262 326 375 280 272 242 410 325 120 230 197
Labelled Cell Count 244 413 342 227 251 427 505 446 371 466 410 457 211 268 334 412 307 297 242 410 325 120 230 197


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 7.1 6.4 6.6 6.3 5.5 5.0 5.2 5.1 4.5 4.9 4.7 6.8 6.7 6.7 7.8 9.4 9.0 6.0 6.2 7.2
Std Deviation (1e-4) 0.6 0.8 1.5 2.7 1.6 1.1 1.4 1.4 1.1 1.1 1.5 1.4 0.9 0.9 1.2 1.6 1.6 1.5 1.1 1.1 1.4
Intensity Change (Log2) – – – 0.04 -0.03 -0.23 -0.36 -0.3 -0.33 -0.5 -0.39 -0.45 0.07 0.05 0.05 0.28 0.54 0.48 -0.11 -0.05 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.2 0 0.6 -0.8 0 1.6 0 1.4 0 0 -0.2 0 0 1.3 2.0
Cytoplasm -2.2 -2.9 -5.6 -9.5 -8.9 -10.9 -12.3 -14.3 -14.1 -0.4 2.0 0.3 -2.2 -4.5 -4.0
Endoplasmic Reticulum 0 0 0 0 2.6 2.7 0 2.6 0 0 0 0 2.9 2.4 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 4.2 9.8 11.6 9.8 14.0 0 16.9 0 0 0 0 2.3 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.3 0 0 0 3.8 3.5 0 0 0 0 4.8 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.249 1.5613 1.7823 1.4577 1.7366 1.9755 3.9406 4.5403 4.2166 4.3554 4.317 4.2688 4.8786 5.3232 5.2782 5.7642 5.2913 5.3003
Actin 0.0197 0.0018 0.0098 0.0009 0.0002 0.001 0.0174 0.004 0.0097 0.0023 0.0295 0.0152 0.0263 0.001 0.0206 0.0259 0.0258 0.0029
Bud 0.0004 0.0001 0.0011 0.0002 0 0.0001 0.0012 0.0012 0.0009 0.0021 0.0016 0.0019 0.0009 0.0019 0.0105 0.0005 0.0005 0.0013
Bud Neck 0.0021 0.0004 0.0014 0.0002 0.0001 0.0059 0.0059 0.0005 0.003 0.0005 0.002 0.0052 0.009 0.0002 0.0064 0.0037 0.001 0.0031
Bud Periphery 0.0011 0.0001 0.0015 0.0003 0 0.0001 0.0022 0.0015 0.0009 0.0037 0.0029 0.0026 0.0009 0.0015 0.0079 0.0008 0.0005 0.0019
Bud Site 0.0023 0.0097 0.0013 0.0003 0 0.0001 0.0105 0.0089 0.0067 0.002 0.0059 0.0013 0.0033 0.0101 0.0102 0.0016 0.0007 0.002
Cell Periphery 0.0031 0.001 0.0005 0.0005 0.0001 0.0003 0.001 0.0008 0.0003 0.0002 0.0006 0.0002 0.0006 0.0004 0.0008 0.0004 0.0001 0.0002
Cytoplasm 0.518 0.7499 0.6553 0.8468 0.8213 0.838 0.7077 0.7292 0.7438 0.812 0.6539 0.7428 0.6192 0.8119 0.7113 0.6691 0.7157 0.772
Cytoplasmic Foci 0.0077 0.0066 0.0098 0.0037 0.0046 0.0063 0.0156 0.0109 0.0095 0.0172 0.0545 0.0239 0.0247 0.0081 0.0118 0.0172 0.0333 0.0049
Eisosomes 0.0016 0.0001 0.0001 0 0 0 0.0002 0.0003 0.0002 0 0.0003 0.0001 0.0005 0.0001 0.0005 0.0002 0.0002 0.0003
Endoplasmic Reticulum 0.025 0.0136 0.0089 0.0064 0.0024 0.0062 0.0072 0.0026 0.0041 0.0017 0.0035 0.0024 0.0096 0.0025 0.0062 0.0031 0.0044 0.0029
Endosome 0.0081 0.0176 0.0072 0.0024 0.0026 0.0026 0.0156 0.0037 0.0067 0.0189 0.0195 0.0066 0.0313 0.0019 0.0074 0.023 0.0277 0.0023
Golgi 0.0033 0.0014 0.0014 0.0003 0.0002 0.0003 0.0031 0.0006 0.0037 0.0011 0.0053 0.0039 0.0055 0.0003 0.0023 0.0015 0.0098 0.0003
Lipid Particles 0.0034 0.0003 0.0009 0.0001 0.0001 0.0003 0.0021 0.0003 0.0017 0.0001 0.0113 0.0024 0.0044 0.0003 0.0014 0.0009 0.0037 0.0004
Mitochondria 0.0016 0.0002 0.0004 0.0001 0.0001 0.0002 0.007 0.0004 0.0029 0.0008 0.0025 0.0011 0.0022 0.0025 0.0062 0.0003 0.0021 0.0002
None 0.3922 0.1909 0.2919 0.1335 0.1669 0.1363 0.19 0.2295 0.1954 0.1312 0.157 0.1814 0.2071 0.1489 0.182 0.2446 0.1591 0.2006
Nuclear Periphery 0.0038 0.0017 0.0021 0.0009 0.0002 0.0004 0.0025 0.0006 0.001 0.0006 0.0025 0.0011 0.025 0.0004 0.0015 0.0006 0.0021 0.0008
Nucleolus 0.0005 0.0001 0.0001 0 0 0 0.0004 0.0002 0.0003 0.0001 0.0018 0.0001 0.001 0.0039 0.0006 0.0001 0.0001 0.0005
Nucleus 0.0021 0.0014 0.002 0.0012 0.0003 0.0006 0.0025 0.0017 0.0015 0.0014 0.0028 0.0014 0.0089 0.0016 0.0021 0.0007 0.001 0.0013
Peroxisomes 0.0005 0.0001 0.0003 0 0 0.0001 0.0014 0.0004 0.003 0.0006 0.0154 0.0034 0.002 0.0003 0.0051 0.0009 0.006 0.0001
Punctate Nuclear 0.001 0.0003 0.0008 0.0003 0.0001 0.0002 0.003 0.0006 0.0028 0.0009 0.0243 0.0018 0.0125 0.0005 0.001 0.0018 0.0011 0.001
Vacuole 0.002 0.0024 0.0026 0.0015 0.0007 0.001 0.0033 0.0021 0.0015 0.0021 0.0027 0.0009 0.004 0.0014 0.0035 0.0027 0.0041 0.0008
Vacuole Periphery 0.0006 0.0004 0.0004 0.0001 0.0001 0.0001 0.0004 0.0002 0.0003 0.0004 0.0005 0.0002 0.0012 0.0002 0.0007 0.0005 0.0011 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.1971 13.3811 10.875 13.6722 11.09 12.1108 10.3083 13.0451 15.3212 14.6656
Translational Efficiency 1.6161 0.9711 0.9976 0.7269 0.9821 1.0846 1.0276 0.8457 0.671 0.8889

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Signal transducing MAP kinase kinase; involved in pheromone response where it phosphorylates Fus3p; involved in the pseudohyphal/invasive growth pathway where it phosphorylates of Kss1p; phosphorylated by Ste11p; degraded by ubiquitin pathway
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ste7

Ste7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ste7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available