Standard name
Human Ortholog
Description Cytoplasmic DEAD-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacting with both decapping and deadenylase complexes; role in translational repression, mRNA decay, and possibly mRNA export; interacts and cooperates with Ngr1p to promote specific mRNA decay; ATP- and RNA-bound form promotes processing body (PB) assembly, while ATPase stimulation by Not1p promotes PB disassembly; forms cytoplasmic foci on replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0.05 0 0 0.13 0.2 0.07 0.11 0.17 0.14 0.19 0.14 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0.11 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0.08 0 0.05 0.08 0.11 0.15 0.19 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.97 0.95 0.89 0.83 0.76 0.62 0.58 0.49 0.56 0.43 0.45 0.76 0.62 0.63 0.93 0.89 0.96 0.91 0.94 0.75 0.9 0.87 0.82
Endoplasmic Reticulum 0 0 0 0 0.07 0.1 0.15 0.13 0.24 0.17 0.17 0.18 0 0.06 0 0 0 0 0.06 0 0.15 0.07 0.09 0.14
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.22 0.19 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.06 0.06 0.14 0.17 0.23 0.16 0.23 0.37 0.26 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.07 0.07 0 0 0.06 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 8 2 4 17 19 9 13 20 31 53 37 1 0 0 0 1 0 0 0 0
Bud 0 2 1 1 2 2 0 2 5 3 3 2 8 18 29 3 2 1 0 2 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3 5 1 0 6 5 0 6 7 14 18 23 5 3 6 7 10 5 0 0 1 0 0 0
Cytoplasm 230 270 87 70 129 48 70 73 46 74 51 54 167 171 164 516 294 239 212 270 83 173 238 204
Endoplasmic Reticulum 2 2 0 1 11 6 17 17 22 23 20 22 7 16 7 11 9 7 13 5 16 13 24 35
Endosome 0 0 0 1 2 0 1 5 1 0 0 0 2 7 13 3 1 0 0 0 0 0 0 1
Golgi 0 1 0 0 3 0 1 4 3 1 0 2 28 60 49 4 7 0 0 0 0 0 1 0
Mitochondria 2 4 4 5 9 9 19 29 15 31 44 31 0 3 4 7 6 2 0 1 2 0 1 0
Nucleus 0 0 2 0 0 1 0 0 1 0 4 1 0 1 0 1 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 1 1 0 0 0 0 1 1 5 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 3 1 0 8 9 2 5 7 3 3 5 4 26 16 7 1 3 2 1 3 5
Unique Cell Count 234 277 92 79 155 63 112 126 93 133 120 121 221 278 260 554 329 250 233 288 111 193 273 251
Labelled Cell Count 237 285 95 82 171 73 121 163 121 160 160 160 254 339 321 579 346 261 233 288 111 193 273 251


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.1 20.1 15.1 13.2 14.5 14.1 11.6 12.4 13.9 12.8 13.0 12.9 21.0 21.5 21.2 20.1 23.5 23.9 19.0 18.7 21.5
Std Deviation (1e-4) 3.7 3.9 3.3 3.3 3.1 3.0 3.7 3.1 3.7 3.5 3.6 3.4 3.5 3.9 4.6 5.6 6.8 6.8 3.4 3.8 4.1
Intensity Change (Log2) -0.19 -0.06 -0.1 -0.38 -0.29 -0.13 -0.24 -0.22 -0.24 0.47 0.51 0.49 0.41 0.63 0.66 0.32 0.31 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 3.7 4.6 0 3.3 4.1 3.8 4.5 3.8 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 3.0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 2.8 3.5 4.1 0 0 0 0 0 0
Cytoplasm -1.4 -2.6 -3.4 -5.4 -6.0 -6.8 -6.4 -7.9 -7.6 -3.9 -6.0 -5.7 -0.5 -1.5 0.4
Endoplasmic Reticulum 0 0 0 3.9 3.7 5.0 4.2 4.1 4.3 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 3.6 4.9 4.5 0 0 0
Mitochondria 0 0 2.2 2.8 3.8 2.6 3.9 5.6 4.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.4409 25.0166 23.2144 20.5867 21.5982 23.1162 14.216 17.7092 15.7434 15.3061 16.2711 17.3215 17.374 22.7582 21.5385 19.9578 20.5743 21.2963
Actin 0.0494 0.0007 0.0033 0.025 0.0004 0.0019 0.0046 0.0094 0.0348 0.0018 0.0012 0.0013 0.009 0.001 0.0033 0.0014 0.0014 0.0017
Bud 0.0008 0.0004 0.0011 0.0008 0.0002 0.0005 0.0004 0.0015 0.0007 0.0008 0.0005 0.0003 0.0008 0.0062 0.0003 0.0005 0.0002 0.0011
Bud Neck 0.0009 0.0004 0.0011 0.0021 0.0012 0.0029 0.0007 0.0003 0.0007 0.0005 0.0004 0.001 0.0013 0.0003 0.0006 0.0007 0.001 0.0014
Bud Periphery 0.0011 0.0003 0.001 0.0007 0.0001 0.0003 0.0002 0.0005 0.0006 0.0003 0.0004 0.0002 0.0007 0.0023 0.0001 0.0003 0.0001 0.0003
Bud Site 0.0021 0.001 0.002 0.0012 0 0.0002 0.0019 0.0043 0.0118 0.0002 0.001 0.0001 0.0021 0.0038 0.0003 0.0007 0.0002 0.0001
Cell Periphery 0.0013 0.0006 0.0009 0.0003 0.0001 0.0002 0.0002 0.0002 0.0005 0.0001 0.0003 0.0001 0.0006 0.0006 0.0001 0.0001 0.0001 0.0002
Cytoplasm 0.5102 0.8763 0.8218 0.6689 0.9059 0.746 0.7128 0.9523 0.7855 0.8369 0.8432 0.8448 0.7 0.9447 0.9027 0.8531 0.8619 0.8028
Cytoplasmic Foci 0.0274 0.0028 0.0118 0.04 0.0043 0.0108 0.0336 0.0059 0.0244 0.0174 0.0422 0.0224 0.0341 0.0036 0.0104 0.0132 0.0126 0.015
Eisosomes 0.0007 0 0.0001 0.0002 0 0 0 0 0.0005 0 0.0001 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.134 0.0681 0.0536 0.0359 0.0382 0.1459 0.1292 0.014 0.03 0.0067 0.01 0.0448 0.0945 0.0196 0.0257 0.0127 0.0678 0.0922
Endosome 0.0693 0.0181 0.0292 0.0683 0.0232 0.0482 0.053 0.0049 0.0527 0.1028 0.0552 0.0529 0.0715 0.0061 0.0306 0.044 0.0246 0.0491
Golgi 0.0143 0.0024 0.0027 0.0141 0.001 0.0044 0.0074 0.0012 0.0173 0.0186 0.0043 0.0082 0.0084 0.0013 0.0107 0.0076 0.0017 0.0085
Lipid Particles 0.0195 0.0001 0.0021 0.0271 0 0.0002 0.0085 0.0001 0.0022 0.0011 0.013 0.0033 0.0083 0.0001 0.0006 0.0128 0.0037 0.0002
Mitochondria 0.0303 0.0031 0.0053 0.0112 0.0006 0.0047 0.0023 0.0004 0.0167 0.0016 0.0087 0.0109 0.0057 0.0034 0.0012 0.0269 0.0009 0.005
None 0.0098 0.0004 0.0019 0.0008 0.0003 0.0005 0.0021 0.0004 0.0024 0.0004 0.0041 0.0015 0.0053 0.0003 0.0004 0.0003 0.0039 0.0071
Nuclear Periphery 0.0483 0.011 0.0133 0.0178 0.0085 0.013 0.0174 0.0016 0.0051 0.0015 0.0021 0.0021 0.0164 0.0013 0.0039 0.005 0.0064 0.004
Nucleolus 0.0009 0.0001 0.0006 0.0004 0 0 0 0 0.0001 0 0.0007 0.0001 0.0011 0.0001 0 0.0002 0.0001 0.0005
Nucleus 0.0088 0.0029 0.0053 0.004 0.0033 0.0042 0.0032 0.0009 0.0015 0.0008 0.0013 0.001 0.0046 0.0011 0.0012 0.002 0.0061 0.002
Peroxisomes 0.0205 0 0.0008 0.0132 0 0.0001 0.0089 0.0004 0.0016 0.0002 0.0015 0.0004 0.003 0.0001 0.0002 0.0027 0.0002 0.0002
Punctate Nuclear 0.0084 0.0001 0.0077 0.0027 0.0001 0.0002 0.0009 0.0003 0.0018 0.0002 0.0022 0.0003 0.0081 0.0001 0.0001 0.0003 0.0007 0.0001
Vacuole 0.0317 0.008 0.026 0.0423 0.009 0.0106 0.0101 0.0012 0.0056 0.0052 0.0048 0.0026 0.0196 0.0034 0.0057 0.0095 0.0049 0.0054
Vacuole Periphery 0.0101 0.0036 0.0085 0.0232 0.0034 0.0052 0.0026 0.0003 0.0036 0.0032 0.0028 0.0019 0.0046 0.0007 0.0018 0.0058 0.0015 0.0032

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 95.3962 75.8751 90.9503 116.4351 102.5225 80.48 89.8313 102.4515 89.863 107.7986
Translational Efficiency 1.9706 2.0406 1.8255 1.292 1.5775 2.5443 1.7559 1.4827 1.8505 1.6672

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Cytoplasmic DEAD-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacting with both decapping and deadenylase complexes; role in translational repression, mRNA decay, and possibly mRNA export; interacts and cooperates with Ngr1p to promote specific mRNA decay; ATP- and RNA-bound form promotes processing body (PB) assembly, while ATPase stimulation by Not1p promotes PB disassembly; forms cytoplasmic foci on replication stress
Localization
Cell Percentages cytoplasm (90%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Dhh1

Dhh1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dhh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available