Standard name | |
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Human Ortholog | |
Description | Cytoplasmic DEAD-box helicase, stimulates mRNA decapping; coordinates distinct steps in mRNA function and decay, interacting with both decapping and deadenylase complexes; role in translational repression, mRNA decay, and possibly mRNA export; interacts and cooperates with Ngr1p to promote specific mRNA decay; ATP- and RNA-bound form promotes processing body (PB) assembly, while ATPase stimulation by Not1p promotes PB disassembly; forms cytoplasmic foci on replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 8 | 2 | 4 | 17 | 19 | 9 | 13 | 20 | 31 | 53 | 37 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 0 | 2 | 1 | 1 | 2 | 2 | 0 | 2 | 5 | 3 | 3 | 2 | 8 | 18 | 29 | 3 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 5 | 1 | 0 | 6 | 5 | 0 | 6 | 7 | 14 | 18 | 23 | 5 | 3 | 6 | 7 | 10 | 5 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 230 | 270 | 87 | 70 | 129 | 48 | 70 | 73 | 46 | 74 | 51 | 54 | 167 | 171 | 164 | 516 | 294 | 239 | 212 | 270 | 83 | 173 | 238 | 204 |
Endoplasmic Reticulum | 2 | 2 | 0 | 1 | 11 | 6 | 17 | 17 | 22 | 23 | 20 | 22 | 7 | 16 | 7 | 11 | 9 | 7 | 13 | 5 | 16 | 13 | 24 | 35 |
Endosome | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 5 | 1 | 0 | 0 | 0 | 2 | 7 | 13 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Golgi | 0 | 1 | 0 | 0 | 3 | 0 | 1 | 4 | 3 | 1 | 0 | 2 | 28 | 60 | 49 | 4 | 7 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 2 | 4 | 4 | 5 | 9 | 9 | 19 | 29 | 15 | 31 | 44 | 31 | 0 | 3 | 4 | 7 | 6 | 2 | 0 | 1 | 2 | 0 | 1 | 0 |
Nucleus | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 4 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 3 | 1 | 0 | 8 | 9 | 2 | 5 | 7 | 3 | 3 | 5 | 4 | 26 | 16 | 7 | 1 | 3 | 2 | 1 | 3 | 5 |
Unique Cell Count | 234 | 277 | 92 | 79 | 155 | 63 | 112 | 126 | 93 | 133 | 120 | 121 | 221 | 278 | 260 | 554 | 329 | 250 | 233 | 288 | 111 | 193 | 273 | 251 |
Labelled Cell Count | 237 | 285 | 95 | 82 | 171 | 73 | 121 | 163 | 121 | 160 | 160 | 160 | 254 | 339 | 321 | 579 | 346 | 261 | 233 | 288 | 111 | 193 | 273 | 251 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 20.1 | 20.1 | 15.1 | 13.2 | 14.5 | 14.1 | 11.6 | 12.4 | 13.9 | 12.8 | 13.0 | 12.9 | 21.0 | 21.5 | 21.2 | 20.1 | 23.5 | 23.9 | 19.0 | 18.7 | 21.5 |
Std Deviation (1e-4) | 3.7 | 3.9 | 3.3 | 3.3 | 3.1 | 3.0 | 3.7 | 3.1 | 3.7 | 3.5 | 3.6 | 3.4 | 3.5 | 3.9 | 4.6 | 5.6 | 6.8 | 6.8 | 3.4 | 3.8 | 4.1 |
Intensity Change (Log2) | – | – | – | -0.19 | -0.06 | -0.1 | -0.38 | -0.29 | -0.13 | -0.24 | -0.22 | -0.24 | 0.47 | 0.51 | 0.49 | 0.41 | 0.63 | 0.66 | 0.32 | 0.31 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 3.7 | 4.6 | 0 | 3.3 | 4.1 | 3.8 | 4.5 | 3.8 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 3.5 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.4 | -2.6 | -3.4 | -5.4 | -6.0 | -6.8 | -6.4 | -7.9 | -7.6 | -3.9 | -6.0 | -5.7 | -0.5 | -1.5 | 0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 3.9 | 3.7 | 5.0 | 4.2 | 4.1 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.6 | 4.9 | 4.5 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.2 | 2.8 | 3.8 | 2.6 | 3.9 | 5.6 | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 17.4409 | 25.0166 | 23.2144 | 20.5867 | 21.5982 | 23.1162 | 14.216 | 17.7092 | 15.7434 | 15.3061 | 16.2711 | 17.3215 | 17.374 | 22.7582 | 21.5385 | 19.9578 | 20.5743 | 21.2963 |
Actin | 0.0494 | 0.0007 | 0.0033 | 0.025 | 0.0004 | 0.0019 | 0.0046 | 0.0094 | 0.0348 | 0.0018 | 0.0012 | 0.0013 | 0.009 | 0.001 | 0.0033 | 0.0014 | 0.0014 | 0.0017 |
Bud | 0.0008 | 0.0004 | 0.0011 | 0.0008 | 0.0002 | 0.0005 | 0.0004 | 0.0015 | 0.0007 | 0.0008 | 0.0005 | 0.0003 | 0.0008 | 0.0062 | 0.0003 | 0.0005 | 0.0002 | 0.0011 |
Bud Neck | 0.0009 | 0.0004 | 0.0011 | 0.0021 | 0.0012 | 0.0029 | 0.0007 | 0.0003 | 0.0007 | 0.0005 | 0.0004 | 0.001 | 0.0013 | 0.0003 | 0.0006 | 0.0007 | 0.001 | 0.0014 |
Bud Periphery | 0.0011 | 0.0003 | 0.001 | 0.0007 | 0.0001 | 0.0003 | 0.0002 | 0.0005 | 0.0006 | 0.0003 | 0.0004 | 0.0002 | 0.0007 | 0.0023 | 0.0001 | 0.0003 | 0.0001 | 0.0003 |
Bud Site | 0.0021 | 0.001 | 0.002 | 0.0012 | 0 | 0.0002 | 0.0019 | 0.0043 | 0.0118 | 0.0002 | 0.001 | 0.0001 | 0.0021 | 0.0038 | 0.0003 | 0.0007 | 0.0002 | 0.0001 |
Cell Periphery | 0.0013 | 0.0006 | 0.0009 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0006 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Cytoplasm | 0.5102 | 0.8763 | 0.8218 | 0.6689 | 0.9059 | 0.746 | 0.7128 | 0.9523 | 0.7855 | 0.8369 | 0.8432 | 0.8448 | 0.7 | 0.9447 | 0.9027 | 0.8531 | 0.8619 | 0.8028 |
Cytoplasmic Foci | 0.0274 | 0.0028 | 0.0118 | 0.04 | 0.0043 | 0.0108 | 0.0336 | 0.0059 | 0.0244 | 0.0174 | 0.0422 | 0.0224 | 0.0341 | 0.0036 | 0.0104 | 0.0132 | 0.0126 | 0.015 |
Eisosomes | 0.0007 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.134 | 0.0681 | 0.0536 | 0.0359 | 0.0382 | 0.1459 | 0.1292 | 0.014 | 0.03 | 0.0067 | 0.01 | 0.0448 | 0.0945 | 0.0196 | 0.0257 | 0.0127 | 0.0678 | 0.0922 |
Endosome | 0.0693 | 0.0181 | 0.0292 | 0.0683 | 0.0232 | 0.0482 | 0.053 | 0.0049 | 0.0527 | 0.1028 | 0.0552 | 0.0529 | 0.0715 | 0.0061 | 0.0306 | 0.044 | 0.0246 | 0.0491 |
Golgi | 0.0143 | 0.0024 | 0.0027 | 0.0141 | 0.001 | 0.0044 | 0.0074 | 0.0012 | 0.0173 | 0.0186 | 0.0043 | 0.0082 | 0.0084 | 0.0013 | 0.0107 | 0.0076 | 0.0017 | 0.0085 |
Lipid Particles | 0.0195 | 0.0001 | 0.0021 | 0.0271 | 0 | 0.0002 | 0.0085 | 0.0001 | 0.0022 | 0.0011 | 0.013 | 0.0033 | 0.0083 | 0.0001 | 0.0006 | 0.0128 | 0.0037 | 0.0002 |
Mitochondria | 0.0303 | 0.0031 | 0.0053 | 0.0112 | 0.0006 | 0.0047 | 0.0023 | 0.0004 | 0.0167 | 0.0016 | 0.0087 | 0.0109 | 0.0057 | 0.0034 | 0.0012 | 0.0269 | 0.0009 | 0.005 |
None | 0.0098 | 0.0004 | 0.0019 | 0.0008 | 0.0003 | 0.0005 | 0.0021 | 0.0004 | 0.0024 | 0.0004 | 0.0041 | 0.0015 | 0.0053 | 0.0003 | 0.0004 | 0.0003 | 0.0039 | 0.0071 |
Nuclear Periphery | 0.0483 | 0.011 | 0.0133 | 0.0178 | 0.0085 | 0.013 | 0.0174 | 0.0016 | 0.0051 | 0.0015 | 0.0021 | 0.0021 | 0.0164 | 0.0013 | 0.0039 | 0.005 | 0.0064 | 0.004 |
Nucleolus | 0.0009 | 0.0001 | 0.0006 | 0.0004 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0007 | 0.0001 | 0.0011 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0005 |
Nucleus | 0.0088 | 0.0029 | 0.0053 | 0.004 | 0.0033 | 0.0042 | 0.0032 | 0.0009 | 0.0015 | 0.0008 | 0.0013 | 0.001 | 0.0046 | 0.0011 | 0.0012 | 0.002 | 0.0061 | 0.002 |
Peroxisomes | 0.0205 | 0 | 0.0008 | 0.0132 | 0 | 0.0001 | 0.0089 | 0.0004 | 0.0016 | 0.0002 | 0.0015 | 0.0004 | 0.003 | 0.0001 | 0.0002 | 0.0027 | 0.0002 | 0.0002 |
Punctate Nuclear | 0.0084 | 0.0001 | 0.0077 | 0.0027 | 0.0001 | 0.0002 | 0.0009 | 0.0003 | 0.0018 | 0.0002 | 0.0022 | 0.0003 | 0.0081 | 0.0001 | 0.0001 | 0.0003 | 0.0007 | 0.0001 |
Vacuole | 0.0317 | 0.008 | 0.026 | 0.0423 | 0.009 | 0.0106 | 0.0101 | 0.0012 | 0.0056 | 0.0052 | 0.0048 | 0.0026 | 0.0196 | 0.0034 | 0.0057 | 0.0095 | 0.0049 | 0.0054 |
Vacuole Periphery | 0.0101 | 0.0036 | 0.0085 | 0.0232 | 0.0034 | 0.0052 | 0.0026 | 0.0003 | 0.0036 | 0.0032 | 0.0028 | 0.0019 | 0.0046 | 0.0007 | 0.0018 | 0.0058 | 0.0015 | 0.0032 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 95.3962 | 75.8751 | 90.9503 | 116.4351 | 102.5225 | 80.48 | 89.8313 | 102.4515 | 89.863 | 107.7986 |
Translational Efficiency | 1.9706 | 2.0406 | 1.8255 | 1.292 | 1.5775 | 2.5443 | 1.7559 | 1.4827 | 1.8505 | 1.6672 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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