Standard name
Human Ortholog
Description Epsin-like protein involved in endocytosis and actin patch assembly; functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; relocalizes from bud neck to cytoplasm upon DNA replication stress; ENT1 has a paralog, ENT2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.7 0.71 0.76 0.68 0.53 0.46 0.43 0.46 0.46 0.42 0.39 0.3 0.66 0.59 0.53 0.77 0.71 0.53 0.77 0.77 0.86 0.54 0.55 0.72
Bud 0.19 0.21 0.19 0.25 0.28 0.17 0.16 0.2 0.22 0.16 0.18 0.17 0.16 0.12 0.09 0.1 0.09 0.08 0.05 0.08 0 0.19 0.2 0.1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0 0.17 0.12 0.07
Bud Site 0.23 0.12 0.18 0.16 0.1 0.07 0 0.06 0.05 0.06 0.05 0 0.05 0 0 0.05 0 0 – – – – – –
Cell Periphery 0.14 0.14 0.19 0.13 0.08 0.12 0.11 0.14 0.13 0.11 0.13 0.1 0.21 0.22 0.25 0.18 0.2 0.15 0 0 0 0 0 0
Cytoplasm 0.07 0.08 0 0.08 0.1 0.07 0.05 0 0 0 0 0 0.14 0.2 0.2 0 0.14 0.16 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0.13 0 0.05 0.06 0.09 0.07 0.08 0.07 0.1 0.05 0.07 0.14 0.2 0.23 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0.05 0.08 0 0.33 0.32 0.27 0 0 0 0 0 0
Mitochondria 0.37 0.13 0.16 0.25 0.23 0.55 0.66 0.47 0.64 0.74 0.7 0.75 0 0 0 0.05 0.09 0.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.12 0.07 0.28 0.19 0.15 0.19 0.14 0.14 0.11 0.08 0.12 0.07 0.1 0 0.08 0.07 0.08 0.05 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 354 295 357 150 152 194 288 270 239 259 189 161 270 246 257 47 60 52 420 389 450 293 315 457
Bud 95 87 91 55 80 71 105 114 114 101 86 91 65 48 46 6 8 8 27 38 15 105 115 63
Bud Neck 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 42 30 9 93 67 47
Bud Site 115 52 83 35 30 31 29 34 26 38 24 21 19 2 1 3 1 0 – – – – – –
Cell Periphery 69 60 90 29 23 51 74 84 67 69 64 56 88 92 123 11 17 15 22 14 21 5 8 13
Cytoplasm 33 33 18 17 30 29 34 21 9 11 11 12 58 83 97 1 12 16 2 3 0 2 11 5
Endoplasmic Reticulum 23 55 15 10 18 38 48 45 39 59 26 40 56 82 112 4 2 3 2 8 4 7 9 12
Endosome 2 4 4 1 14 3 7 19 3 0 2 1 3 3 9 1 0 3 7 5 5 11 6 6
Golgi 2 6 18 3 2 7 12 30 24 11 13 6 21 33 21 20 27 26 3 4 3 1 1 5
Mitochondria 185 54 77 54 65 233 445 276 334 454 343 407 5 1 3 3 8 10 6 4 3 6 9 9
Nucleus 3 0 0 0 1 0 1 1 0 3 1 0 2 0 2 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1 0 8 10 9 4 8 4 8 0 2 0 0 0 0 3 1 0 1 3 1
Nucleolus 4 2 0 1 3 4 7 8 6 4 5 6 3 1 0 0 1 0 0 0 0 0 0 0
Peroxisomes 61 28 129 41 42 78 94 81 57 49 58 40 40 13 37 4 7 5 4 1 8 2 2 2
SpindlePole 8 2 14 6 8 3 6 18 6 3 4 5 13 23 10 1 1 3 1 0 1 0 0 0
Vac/Vac Membrane 2 0 2 2 4 2 3 5 7 4 3 8 8 14 8 2 3 3 1 2 0 9 11 5
Unique Cell Count 504 416 468 219 287 420 672 583 522 617 488 540 411 414 489 61 85 98 550 507 527 546 569 638
Labelled Cell Count 957 678 899 405 472 752 1163 1016 935 1073 833 862 651 643 726 103 148 144 550 507 527 546 569 638


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 10.9 9.2 9.1 8.7 8.1 7.8 8.0 8.0 7.6 7.4 6.8 10.9 10.9 10.6 13.7 12.7 14.0 10.7 10.7 11.5
Std Deviation (1e-4) 1.4 2.0 1.6 2.2 2.2 2.0 1.9 1.8 1.9 1.5 1.7 1.7 1.7 1.9 1.8 3.1 2.4 3.3 1.7 1.6 1.8
Intensity Change (Log2) – – – -0.01 -0.09 -0.19 -0.24 -0.21 -0.2 -0.28 -0.31 -0.45 0.25 0.24 0.21 0.57 0.46 0.6 0.21 0.22 0.32

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP700051015WT3HU80HU120HU160051015WT3rpd3Δ_1rpd3Δ_2rpd3Δ_3051015WT1AF100AF140AF180051015
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.2 -6.6 -9.2 -11.2 -9.8 -9.8 -11.3 -11.7 -14.7 -3.5 -5.4 -7.7 0.1 -1.1 -4.7
Bud 1.7 2.7 -1.0 -1.7 0 0.9 -1.3 -0.7 -1.1 -1.4 -3.2 -4.4 -1.8 -2.2 -2.7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site -0.6 -2.7 -4.6 -7.5 -6.1 -6.4 -6.0 -6.3 -7.2 -6.1 -8.7 -9.6 -2.6 -3.9 -4.5
Cell Periphery -1.9 -4.2 -2.9 -3.9 -2.1 -2.8 -3.7 -2.6 -4.0 0.8 1.1 2.2 -0.2 0.2 -0.9
Cytoplasm 2.2 3.6 2.0 1.0 -0.2 -2.0 -2.1 -1.4 -1.5 5.4 7.5 7.6 0 0 4.7
Endoplasmic Reticulum 0.9 2.0 3.7 2.9 3.1 3.0 4.1 1.6 2.9 5.7 7.9 9.0 0 0 0
Endosome 0 3.5 0 0 2.6 0 0 0 0 0 0 1.3 0 0 0
Golgi -1.8 -2.6 -2.0 -2.1 1.0 0.6 -2.1 -1.0 -2.8 0.9 2.6 0.4 0 8.7 7.6
Mitochondria 2.6 2.1 12.2 16.6 10.5 15.2 18.6 16.8 18.7 -7.7 -8.5 -8.8 -2.4 -1.7 -1.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -2.5 -4.1 -3.2 -5.7 -5.5 -6.7 -8.6 -6.1 -8.5 -6.7 -9.8 -8.2 -3.6 -3.8 -4.8
SpindlePole -0.2 -0.2 -2.5 -2.7 0.1 -2.1 -3.3 -2.5 -2.4 0.1 1.9 -0.9 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 3.3 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.3067 7.983 8.5159 7.1315 7.4917 8.0315 9.0583 8.3466 9.7338 8.89 8.3584 9.1365 8.7729 8.3143 8.6337 8.4333 8.2709 8.4884
Actin 0.8135 0.7184 0.7912 0.8673 0.887 0.7541 0.6968 0.571 0.7027 0.794 0.7733 0.6994 0.7954 0.6164 0.8111 0.833 0.8995 0.802
Bud 0.0038 0.0058 0.0196 0.0117 0.0078 0.0018 0.0062 0.0094 0.0256 0.0154 0.0087 0.0044 0.0028 0.004 0.0177 0.0168 0.009 0.0014
Bud Neck 0.0162 0.0071 0.0208 0.0104 0.009 0.19 0.0327 0.0101 0.0346 0.0129 0.0149 0.2114 0.0268 0.0114 0.0268 0.0038 0.0046 0.1461
Bud Periphery 0.0092 0.007 0.0557 0.0607 0.0799 0.008 0.022 0.0171 0.0867 0.0807 0.0609 0.0217 0.0085 0.0044 0.0502 0.0918 0.0487 0.0061
Bud Site 0.0431 0.2037 0.0656 0.0023 0.0026 0.0037 0.082 0.3157 0.1076 0.0122 0.0322 0.011 0.0661 0.3001 0.0698 0.0089 0.0054 0.0038
Cell Periphery 0.0065 0.005 0.0071 0.0051 0.0084 0.0042 0.0237 0.0096 0.0086 0.0032 0.0059 0.0125 0.0185 0.0033 0.005 0.004 0.0049 0.0057
Cytoplasm 0.0149 0.0224 0.0189 0.0012 0.0012 0.0062 0.0147 0.0374 0.0122 0.0677 0.0031 0.0032 0.0145 0.0436 0.0064 0.0237 0.0021 0.001
Cytoplasmic Foci 0.0078 0.0082 0.0023 0.0013 0.0002 0.0013 0.0078 0.0056 0.0028 0.0011 0.0082 0.0022 0.0022 0.0036 0.0006 0.0011 0.0007 0.0007
Eisosomes 0.0078 0.003 0.0025 0.0022 0.0015 0.0048 0.0758 0.0087 0.0102 0.0065 0.0114 0.0171 0.0467 0.0026 0.0085 0.0117 0.0219 0.028
Endoplasmic Reticulum 0.0047 0.0055 0.0019 0.0003 0.0005 0.0011 0.0054 0.0013 0.0009 0.0012 0.0019 0.0008 0.002 0.0004 0.0002 0.0006 0.0004 0.0002
Endosome 0.0116 0.0032 0.0022 0.0019 0.0001 0.0038 0.0058 0.0014 0.0011 0.0003 0.0052 0.0014 0.0021 0.0007 0.0001 0.0003 0.0002 0.0002
Golgi 0.0151 0.0071 0.0033 0.0026 0.0008 0.0049 0.0096 0.0078 0.0023 0.0019 0.0046 0.0051 0.0043 0.0076 0.0019 0.0023 0.0017 0.0028
Lipid Particles 0.0051 0.0007 0.0022 0.0088 0 0.001 0.003 0.0009 0.0004 0.0001 0.0218 0.0015 0.0013 0.0003 0.0001 0.0001 0.0003 0.0002
Mitochondria 0.0083 0.0006 0.0009 0.0173 0.0002 0.0028 0.0031 0.0004 0.0005 0.0008 0.0047 0.0012 0.001 0.0002 0.0001 0.0001 0.0002 0.0006
None 0.0151 0.0002 0.0016 0.0002 0.0001 0.0014 0.0011 0.0003 0.0004 0.0004 0.0049 0.0002 0.0011 0.0002 0.0002 0.0001 0.0002 0.0001
Nuclear Periphery 0.0071 0.0002 0.0009 0.0005 0.0001 0.0064 0.0017 0.0005 0.0003 0.0002 0.0045 0.0015 0.0022 0.0001 0.0001 0.0003 0.0001 0
Nucleolus 0.0007 0 0.0001 0.0001 0 0.0003 0.0002 0.0001 0.0001 0 0.0064 0.0006 0.0002 0 0.0001 0 0 0
Nucleus 0.0016 0.0001 0.0004 0.0001 0 0.0013 0.0004 0.0004 0.0004 0.0003 0.0077 0.0014 0.001 0.0002 0.0001 0.0003 0 0
Peroxisomes 0.0035 0.0015 0.0014 0.0052 0.0005 0.0008 0.0051 0.0011 0.0019 0.0007 0.0136 0.0016 0.0007 0.0008 0.0008 0.0002 0.0002 0.0007
Punctate Nuclear 0.0026 0.0002 0.0007 0.0003 0 0.0012 0.0007 0.0005 0.0002 0.0001 0.0036 0.0006 0.0016 0.0002 0.0001 0.0001 0 0
Vacuole 0.0011 0.0001 0.0006 0.0002 0.0001 0.0003 0.0019 0.0006 0.0004 0.0002 0.0015 0.0009 0.0008 0.0001 0.0003 0.0007 0.0001 0.0001
Vacuole Periphery 0.0006 0 0.0001 0.0002 0 0.0005 0.0003 0.0001 0.0001 0 0.0009 0.0002 0.0001 0 0 0.0001 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.4005 16.6203 10.1881 29.7757 25.1536 18.5819 17.17 23.4238 26.3847 26.6452
Translational Efficiency 2.9712 2.174 4.0416 1.6068 1.8435 3.5488 1.7439 1.4749 1.9304 2.0313

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1830 391 1666 1546 1695 1885 130 345 3525 2276 1796 1891

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 837.40 1160.36 1152.71 1321.99 929.45 1055.17 1333.74 1462.26 881.66 1073.24 1165.81 1347.58
Standard Deviation 126.47 161.92 193.68 227.99 134.80 159.10 194.44 256.68 138.41 164.45 199.34 239.69
Intensity Change Log 2 — 0.470584 0.461041 0.658722 — 0.183026 0.521028 0.653751 — 0.326472 0.492908 0.656109

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.679693 0.637991 0.454984 0.518856 0.661998 0.633782 0.500902 0.385805 0.671184 0.634505 0.458308 0.494582
Bud Neck 0.024617 0.049194 0.051411 0.034766 0.035026 0.035793 0.080382 0.033277 0.029623 0.038095 0.053508 0.034494
Bud Site 0.097320 0.140673 0.358345 0.205295 0.116119 0.150902 0.258362 0.193503 0.106360 0.149145 0.351108 0.203144
Cell Periphery 0.042444 0.093794 0.061679 0.126832 0.092459 0.106376 0.059538 0.182435 0.066494 0.104215 0.061524 0.136976
Cytoplasm 0.010413 0.002954 0.015373 0.002124 0.008979 0.006983 0.007066 0.001178 0.009724 0.006291 0.014772 0.001952
Cytoplasmic Foci 0.011463 0.003902 0.010294 0.001029 0.006009 0.005594 0.009526 0.001353 0.008840 0.005303 0.010238 0.001088
Eisosomes 0.024752 0.036285 0.013560 0.083214 0.037060 0.029808 0.030814 0.165499 0.030670 0.030920 0.014809 0.098226
Endoplasmic Reticulum 0.005986 0.006850 0.004740 0.008598 0.004625 0.003204 0.009953 0.011946 0.005332 0.003830 0.005117 0.009209
Endosome 0.014019 0.007202 0.004341 0.003219 0.005135 0.004907 0.008664 0.004481 0.009747 0.005301 0.004654 0.003449
Golgi 0.015761 0.007145 0.003316 0.003685 0.004897 0.004635 0.002765 0.005607 0.010537 0.005066 0.003276 0.004035
Lipid Particles 0.015048 0.001303 0.004634 0.001525 0.003654 0.001833 0.007123 0.006630 0.009570 0.001742 0.004814 0.002456
Mitochondria 0.010720 0.005754 0.004914 0.005779 0.006542 0.007548 0.000802 0.004940 0.008711 0.007239 0.004616 0.005626
Mitotic Spindle 0.000295 0.000565 0.001631 0.000786 0.000348 0.000281 0.000538 0.000665 0.000320 0.000329 0.001552 0.000764
None 0.001024 0.000062 0.000092 0.000076 0.000192 0.000326 0.000027 0.000091 0.000624 0.000281 0.000088 0.000079
Nuclear Periphery 0.000018 0.000021 0.000023 0.000092 0.000019 0.000013 0.000044 0.000090 0.000019 0.000015 0.000024 0.000091
Nuclear Periphery Foci 0.000097 0.000101 0.000391 0.000159 0.000058 0.000089 0.000675 0.000101 0.000078 0.000091 0.000412 0.000148
Nucleolus 0.000041 0.000007 0.000020 0.000033 0.000008 0.000246 0.000006 0.000007 0.000025 0.000205 0.000019 0.000028
Nucleus 0.001023 0.000130 0.000639 0.000240 0.000366 0.000420 0.001442 0.000175 0.000707 0.000370 0.000697 0.000228
Peroxisomes 0.044439 0.004917 0.005333 0.000736 0.015815 0.006415 0.004933 0.000346 0.030675 0.006158 0.005304 0.000665
Vacuole 0.000488 0.000928 0.004177 0.001533 0.000550 0.000549 0.016360 0.001435 0.000518 0.000614 0.005059 0.001515
Vacuole Periphery 0.000339 0.000221 0.000103 0.001424 0.000140 0.000298 0.000077 0.000436 0.000243 0.000285 0.000101 0.001244

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.27 19.61 13.87 6.26 -5.24 2.52 5.53 13.55 12.23 2.98 4.10 21.39 18.07 13.03 -3.28
Bud Neck -4.22 -8.33 -4.07 2.49 5.22 -0.24 -3.58 0.41 0.61 3.64 -3.40 -7.88 -2.27 1.42 6.18
Bud Site -3.57 -28.08 -13.10 -4.90 14.38 -4.70 -5.50 -4.94 -2.70 2.13 -7.23 -28.90 -13.64 -6.87 14.83
Cell Periphery -5.61 -4.57 -14.62 -3.22 -10.56 -2.37 3.18 -6.27 -5.30 -7.32 -8.43 1.34 -12.77 -5.24 -12.85
Cytoplasm 6.38 -3.16 9.10 0.94 8.31 1.44 -0.43 6.67 5.90 1.99 3.42 -3.59 10.39 5.37 8.65
Cytoplasmic Foci 5.52 0.41 10.36 2.94 6.46 0.39 -1.29 4.55 4.37 2.47 3.82 -1.25 11.23 6.01 6.81
Eisosomes -2.79 6.03 -14.24 -8.66 -17.57 2.61 0.97 -10.15 -10.76 -9.51 -0.12 9.34 -16.34 -15.79 -20.43
Endoplasmic Reticulum -0.59 1.26 -1.94 -0.98 -2.70 2.30 -1.77 -3.11 -3.70 -0.48 2.59 0.21 -3.42 -4.64 -3.12
Endosome 3.13 7.63 8.21 2.04 1.51 0.24 -1.20 0.40 0.26 1.30 4.76 5.90 7.06 2.16 1.68
Golgi 3.19 8.68 7.17 1.36 -0.34 0.23 2.03 -0.23 -0.31 -0.92 4.93 7.91 5.09 0.82 -0.71
Lipid Particles 10.20 6.58 9.56 -0.41 3.37 2.22 -1.47 -0.88 -1.44 0.20 9.30 4.16 6.69 -0.90 2.34
Mitochondria 2.07 5.01 3.89 -0.03 -0.86 -0.85 6.99 1.09 1.74 -3.37 1.44 4.63 3.21 1.46 -1.11
Mitotic Spindle -1.55 -2.45 -1.92 -0.76 1.45 0.59 -1.01 -1.37 -1.85 -0.41 -0.13 -2.43 -2.05 -2.04 1.49
None 1.87 1.79 1.85 -1.62 1.07 -0.77 5.21 3.27 1.33 -2.94 1.09 1.97 2.04 1.38 0.81
Nuclear Periphery -0.56 -0.96 -1.63 -1.58 -1.49 2.15 -2.10 -3.88 -4.16 -1.39 1.38 -1.50 -1.98 -2.08 -1.74
Nuclear Periphery Foci -0.18 -3.59 -0.60 -0.55 2.04 -0.70 -2.13 -1.03 -0.18 2.03 -0.36 -4.31 -0.83 -0.62 2.68
Nucleolus 1.45 0.67 0.26 -1.23 -0.40 -1.01 0.03 0.64 1.01 0.46 -0.92 0.17 -0.15 0.91 -0.29
Nucleus 1.75 0.60 1.53 -1.85 3.30 -0.35 -1.59 3.10 1.40 1.73 1.08 -0.43 1.76 0.95 3.77
Peroxisomes 15.57 16.16 18.63 4.13 7.03 5.77 5.23 10.91 7.51 3.03 15.61 16.57 21.11 7.76 7.61
Vacuole -1.54 -5.25 -3.13 -1.41 3.76 0.05 -2.62 -2.20 -2.33 2.53 -0.67 -5.63 -3.51 -3.16 4.53
Vacuole Periphery 0.74 2.62 -2.32 -2.52 -2.87 -2.05 1.46 -1.06 -0.48 -1.28 -0.53 2.82 -2.62 -2.49 -3.00
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Epsin-like protein involved in endocytosis and actin patch assembly; functionally redundant with Ent2p; binds clathrin via a clathrin-binding domain motif at C-terminus; relocalizes from bud neck to cytoplasm upon DNA replication stress; ENT1 has a paralog, ENT2, that arose from the whole genome duplication
Localization
Cell Percentages actin (83%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ent1

Ent1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ent1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available