Standard name
Human Ortholog
Description RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication; Air2p and Air1p have nonredundant roles in regulation of substrate specificity of the exosome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0.05 0.05 0 0 0.07 0 0 0 0 0 0.08 0.15 0 0.07 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.07 0 0.05 0 0.25 0.32 0.26 0.52 0.34 0.55 0.36 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.95 0.89 0.96 0.93 0.94 0.91 0.89 0.84 0.78 0.84 0.84 0.83 0.95 0.91 0.81 0.93 0.85 0.79 0.9 0.9 0.89 0.77 0.62 0.52
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0.06 0 0 0 0 0.06 0.08 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0.07 0.1 0.21 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 1 1 4 1 4 3 5 2 0 1 0 0 0 0 3 1 0 3 2 3
Bud Neck 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3
Bud Site 0 0 0 0 0 2 4 5 19 9 13 11 0 0 0 1 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 7 0 9 16 6 14 24 16 19 11 8 7 19 37 4 7 14 0 0 0 3 0 3
Endoplasmic Reticulum 0 0 0 0 1 0 1 0 0 0 0 0 0 1 0 1 2 2 0 0 0 0 1 1
Endosome 0 0 0 0 1 1 0 1 0 0 0 0 0 1 1 0 0 0 1 2 0 2 4 3
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0 0 0
Mitochondria 31 8 2 9 4 62 110 96 213 175 213 144 3 2 1 3 4 6 5 0 0 0 4 8
Nucleus 294 105 55 153 284 230 305 304 323 434 325 336 205 220 198 100 88 99 281 102 77 147 178 167
Nuclear Periphery 4 1 0 0 3 2 3 6 3 4 7 3 2 2 3 2 0 0 0 0 0 0 1 6
Nucleolus 2 2 0 3 0 1 5 10 13 12 26 21 5 1 4 4 6 2 4 0 0 11 22 35
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 0 0 0 6 2 1 1 4 0 2 1 0 0 0 2 0 1 2 6 5
Vac/Vac Membrane 2 2 0 0 1 3 2 2 1 7 4 6 2 1 5 2 6 8 6 3 5 18 59 76
Unique Cell Count 310 118 57 164 303 253 342 364 412 515 386 403 215 243 245 107 103 126 314 114 87 192 286 321
Labelled Cell Count 340 126 57 176 312 308 449 457 594 664 605 535 224 250 251 117 113 132 314 114 87 192 286 321


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 6.0 5.3 5.7 5.8 4.7 4.4 5.0 4.2 4.5 4.1 4.5 6.3 6.6 6.3 8.3 9.0 9.2 5.6 6.1 6.5
Std Deviation (1e-4) 0.7 1.1 0.6 1.6 1.5 1.2 1.1 1.6 0.9 1.2 1.0 0.9 1.1 1.4 1.4 1.7 2.0 2.0 1.3 1.6 1.2
Intensity Change (Log2) – – – 0.12 0.14 -0.17 -0.28 -0.08 -0.35 -0.23 -0.36 -0.23 0.26 0.33 0.24 0.64 0.76 0.79 0.09 0.22 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 3.1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 3.8 0 0 0 0 0 0 0 0 0 0
Nucleus -0.9 -0.8 -1.4 -1.7 -2.6 -3.2 -2.5 -2.5 -2.6 -0.4 -1.5 -2.9 -0.8 -2.2 -3.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7817 2.9134 2.6315 2.5495 2.9027 2.5433 2.0906 2.4186 2.1561 1.593 1.6601 1.9728 1.1219 1.1046 0.801 0.952 0.5929 0.7886
Actin 0.0271 0.0001 0.0143 0.016 0.0116 0.0012 0.0109 0 0.0046 0 0.0045 0.0002 0.0636 0.0002 0.0057 0.016 0.0269 0.0017
Bud 0.0005 0 0.0002 0.0017 0.0013 0.0002 0.0001 0 0.0001 0 0.0002 0.0001 0.0013 0.0003 0.0003 0.0083 0.0002 0.0002
Bud Neck 0.0012 0.0002 0.0007 0.0009 0.001 0.0021 0.0011 0.0001 0.0007 0.0001 0.0032 0.0012 0.0039 0.0002 0.0005 0.0013 0.0008 0.0012
Bud Periphery 0.0006 0.0001 0.0004 0.0025 0.0073 0.0007 0.0002 0 0.0002 0 0.0008 0.0003 0.0025 0.0005 0.0005 0.0401 0.0003 0.0005
Bud Site 0.0013 0.0002 0.001 0.0117 0.0009 0.0003 0.0006 0 0.0003 0 0.0009 0.0001 0.0124 0.0004 0.0124 0.0065 0.0009 0.0002
Cell Periphery 0.0003 0 0.0002 0.0004 0.0005 0.0002 0.0001 0 0.0001 0 0.0002 0.0001 0.0008 0.0001 0.0003 0.0018 0.0001 0.0001
Cytoplasm 0.0096 0.0051 0.0072 0.0021 0.002 0.0008 0.0023 0.0001 0.0007 0.0001 0.0015 0.0001 0.001 0.001 0.0008 0.0016 0.0661 0.0001
Cytoplasmic Foci 0.0129 0.0004 0.0054 0.0105 0.0095 0.001 0.009 0 0.0001 0 0.0008 0 0.0046 0 0.0019 0.0063 0.0224 0.0001
Eisosomes 0.0006 0 0.0002 0.0002 0.0001 0 0.0002 0 0.0001 0 0.0001 0 0.0004 0 0 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0027 0.0003 0.0019 0.0029 0.0045 0.0006 0.0008 0 0.0004 0 0.0012 0.0001 0.0014 0.0001 0.0006 0.0016 0.0019 0.0003
Endosome 0.0168 0.0004 0.0087 0.0357 0.0277 0.0066 0.0082 0 0.001 0 0.0141 0.001 0.017 0.0006 0.009 0.0187 0.0243 0.0022
Golgi 0.0075 0 0.0039 0.0091 0.02 0.0016 0.0021 0 0.0006 0 0.0011 0.0001 0.0141 0.0002 0.0049 0.0152 0.006 0.0031
Lipid Particles 0.0191 0 0.0125 0.0222 0.0918 0.0067 0.0069 0 0.0006 0 0.0023 0.0001 0.0076 0.0002 0.0012 0.0056 0.0277 0.0006
Mitochondria 0.024 0.0005 0.0125 0.0736 0.0164 0.0108 0.0012 0.0002 0.0009 0.0001 0.0054 0.0013 0.0306 0.0106 0.0147 0.0138 0.0061 0.0224
None 0.013 0.0017 0.0116 0.0021 0.0043 0.0006 0.0044 0 0.0003 0 0.0014 0.0003 0.001 0.0007 0.0002 0.0006 0.0032 0.0001
Nuclear Periphery 0.0139 0.0055 0.0074 0.0157 0.0584 0.0072 0.0105 0.0065 0.006 0.0007 0.0406 0.0031 0.0079 0.0069 0.0051 0.012 0.0144 0.0016
Nucleolus 0.0512 0.0728 0.122 0.0699 0.0267 0.1192 0.0527 0.0544 0.1005 0.0361 0.0372 0.1199 0.0347 0.039 0.091 0.1395 0.0205 0.1154
Nucleus 0.7593 0.9071 0.7776 0.6937 0.6609 0.8318 0.8731 0.9348 0.8789 0.9301 0.8327 0.8695 0.7754 0.9353 0.8437 0.6897 0.7439 0.848
Peroxisomes 0.0165 0 0.0058 0.0109 0.0265 0.0006 0.0033 0 0.0001 0 0.0007 0 0.0097 0.0001 0.0014 0.0032 0.0173 0.0003
Punctate Nuclear 0.0166 0.005 0.004 0.0036 0.0075 0.0045 0.0106 0.0033 0.0024 0.0327 0.0431 0.0015 0.0013 0.0008 0.0019 0.0013 0.0105 0.0006
Vacuole 0.0033 0.0003 0.0015 0.0075 0.0102 0.0019 0.0009 0.0001 0.0008 0 0.0054 0.0006 0.0055 0.0016 0.0021 0.0127 0.0038 0.0005
Vacuole Periphery 0.0019 0.0001 0.0011 0.0071 0.0109 0.0011 0.0006 0.0002 0.0004 0 0.0024 0.0005 0.0033 0.0013 0.0016 0.0039 0.0024 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.9957 7.6242 16.2652 10.6315 11.0579 9.5895 15.8994 10.4058 11.0332 13.7729
Translational Efficiency 2.6283 2.5856 0.9898 1.1932 1.6585 2.7033 1.4672 1.8302 1.6504 1.4297

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication; Air2p and Air1p have nonredundant roles in regulation of substrate specificity of the exosome
Localization
Cell Percentages nucleus (96%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Air2

Air2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Air2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available