Standard name
Human Ortholog
Description Subunit A of the V1 peripheral membrane domain of V-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits; involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0.05 0 0.06 0 0.09 0 – – 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 – 0.07 0.14 0.13 0.16 0.27 0.2 0.24 0.34 0.29 0.21 0.24 – – 0.12 0.14 0.25 0.07 0 0 0 0 0
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 – – 0.06 0.07 0.1 0 0 0.08 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0.06 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0.05 0.07 0.08 0.11 0.05 0.09 0.17 0.09 0.15 0.11 0 – – 0.09 0.11 0.2 0 0 0.06 0 0 0
Nucleus 0.07 – 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0.1 0.16 0 0 0.06 0.05
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0.08 0.06 0.05 0.05
Nucleolus 0.06 – 0.09 0.14 0.08 0.1 0.06 0.09 0.05 0 0.11 0.05 0 – – 0.11 0.09 0.13 0 0.07 0 0.06 0.09 0.05
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.72 – 0.86 0.79 0.76 0.68 0.63 0.63 0.63 0.53 0.51 0.58 0.76 – – 0.77 0.77 0.6 0.7 0.61 0.59 0.76 0.71 0.74
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 – – 2 0 0 0 0 1 0 0 0
Bud 0 – 1 0 0 2 2 0 0 0 1 0 0 – – 1 0 0 0 0 0 0 2 1
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 – – – – – –
Cell Periphery 0 – 1 0 2 0 6 11 5 10 6 16 0 – – 1 2 0 0 0 0 0 0 0
Cytoplasm 22 – 13 17 20 24 75 42 35 59 45 39 4 – – 51 46 64 8 1 5 0 1 1
Endoplasmic Reticulum 0 – 3 1 2 2 3 6 3 3 7 5 0 – – 24 23 26 0 0 15 0 1 1
Endosome 0 – 0 0 0 0 0 0 1 0 0 0 0 – – 0 0 0 0 0 1 1 0 0
Golgi 0 – 2 5 9 2 9 6 2 1 3 8 0 – – 18 14 4 1 0 6 1 0 4
Mitochondria 3 – 9 8 12 16 14 19 24 15 24 20 0 – – 37 35 52 0 0 11 2 1 1
Nucleus 8 – 1 0 2 2 1 2 2 3 0 1 0 – – 11 10 4 12 8 6 2 5 5
Nuclear Periphery 0 – 0 1 1 0 0 0 2 0 0 0 0 – – 1 0 0 4 0 15 5 4 6
Nucleolus 7 – 15 17 13 15 18 18 7 6 17 9 0 – – 46 28 32 3 3 5 4 8 6
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 2 0 0 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 78 – 150 96 118 100 175 130 90 93 80 106 13 – – 331 252 154 87 30 107 66 67 90
Unique Cell Count 109 174 121 156 146 277 206 143 176 157 183 17 428 326 255 126 50 184 87 96 122
Labelled Cell Count 118 195 145 179 165 303 234 171 190 183 204 17 523 410 336 126 50 184 87 96 122


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 147.1 – 151.6 146.0 141.9 149.8 142.5 125.0 120.6 112.6 109.5 115.0 120.2 – – 94.5 118.2 157.7 132.4 156.1 164.6
Std Deviation (1e-4) 38.3 – 44.6 48.6 46.3 56.9 48.4 47.6 39.8 32.5 30.5 29.8 28.8 – – 42.0 50.7 67.3 55.8 58.7 71.3
Intensity Change (Log2) – – – -0.05 -0.09 -0.02 -0.09 -0.28 -0.33 -0.43 -0.47 -0.4 -0.33 – – -0.68 -0.36 0.06 -0.2 0.04 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Cell Periphery 0 0 0 0 2.6 0 2.7 0 3.6 0 – – 0 0 0
Cytoplasm 1.8 1.6 2.5 5.1 3.6 4.2 6.0 5.1 3.7 0 – – 1.6 2.2 4.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 – – 2.1 2.6 3.4
Endosome 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Golgi 0 2.3 0 0 0 0 0 0 0 0 – – 1.9 1.9 0
Mitochondria 0.5 0.9 1.9 -0.1 1.5 3.4 1.2 3.1 2.0 0 – – 1.5 2.1 4.4
Nucleus 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Nucleolus 1.5 -0.1 0.5 -0.8 0 -1.3 -2.1 0.7 -1.4 0 – – 0.8 0 1.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 – – 0 0 0
Vacuole -1.6 -2.5 -3.8 -5.3 -5.1 -4.8 -6.8 -7.0 -5.9 -1.1 – – -2.5 -2.4 -5.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0059 0 0.0033 0.0004 0.0024 0.0011 0.0001 0.0003 0.0001 0.0008 0.0005 0 – – – – – –
Bud 0.0002 0 0.0005 0.0186 0.0011 0.0002 0 0.0001 0.0002 0.0002 0.0041 0.0001 – – – – – –
Bud Neck 0.0001 0 0.0002 0.0002 0.0001 0.0005 0 0 0.0001 0.0002 0.0001 0 – – – – – –
Bud Periphery 0.0004 0 0.0004 0.0136 0.0013 0.0005 0.0001 0.0004 0.0008 0.0007 0.0223 0.0002 – – – – – –
Bud Site 0.0003 0 0.0003 0.0223 0.0081 0.001 0.0001 0.0001 0.0001 0.0004 0.0003 0 – – – – – –
Cell Periphery 0.009 0.0039 0.0086 0.0172 0.0085 0.0044 0.0072 0.0075 0.0122 0.0202 0.0275 0.0059 – – – – – –
Cytoplasm 0.0008 0.0012 0.0011 0.0088 0.0005 0.0001 0 0 0 0.0723 0.0001 0 – – – – – –
Cytoplasmic Foci 0.0123 0 0.0017 0.0031 0.0158 0.0026 0 0 0.0001 0.0051 0.0001 0.0001 – – – – – –
Eisosomes 0.0001 0 0.0001 0 0.0002 0 0.0002 0.0002 0.0001 0.0001 0 0 – – – – – –
Endoplasmic Reticulum 0.0002 0.0063 0.0003 0.0002 0.0001 0 0 0.0001 0 0.0068 0.0008 0 – – – – – –
Endosome 0.0055 0.0012 0.0028 0.0032 0.0026 0.004 0.0005 0.0004 0.0006 0.0168 0.0011 0.0005 – – – – – –
Golgi 0.0019 0 0.0016 0.0006 0.0012 0.0047 0.0001 0.0001 0.0001 0.0005 0.0001 0.0001 – – – – – –
Lipid Particles 0.0047 0.0005 0.0071 0.0005 0.0059 0.0026 0.0045 0.0014 0.0059 0.0031 0.0022 0.0047 – – – – – –
Mitochondria 0.0211 0.0016 0.033 0.0033 0.0174 0.007 0.1632 0.0196 0.1421 0.0103 0.0206 0.0279 – – – – – –
None 0.0008 0 0.0005 0.0006 0.0011 0 0 0.0001 0 0.0433 0.0001 0 – – – – – –
Nuclear Periphery 0.0092 0.0052 0.0074 0.0004 0.0019 0.0179 0.0056 0.0099 0.0036 0.0056 0.014 0.0194 – – – – – –
Nucleolus 0.0291 0.0055 0.026 0.0117 0.0486 0.0132 0.0166 0.0358 0.055 0.0466 0.0981 0.039 – – – – – –
Nucleus 0.006 0.0078 0.0042 0.0061 0.0068 0.0038 0.0039 0.0073 0.0071 0.0116 0.0113 0.0102 – – – – – –
Peroxisomes 0.001 0 0.0006 0.0004 0.0035 0.0017 0.0003 0.0001 0.0003 0.0005 0.0002 0.0001 – – – – – –
Punctate Nuclear 0.0023 0 0.0003 0.0002 0.0023 0.0001 0 0 0 0.0034 0 0 – – – – – –
Vacuole 0.1676 0.1681 0.1648 0.3077 0.1666 0.1204 0.1618 0.1354 0.1427 0.26 0.1893 0.1077 – – – – – –
Vacuole Periphery 0.7217 0.7984 0.7354 0.5811 0.704 0.8144 0.6358 0.7812 0.6288 0.4913 0.6072 0.7839 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 567.3132 579.0666 617.0001 607.8102 535.8123 485.5584 585.6487 568.5783 627.6197 579.8575
Translational Efficiency 1.2778 1.3443 1.2224 1.3675 1.3285 1.3843 1.2793 1.059 1.0324 1.0815

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
161 431 1949 442 189 226 2325 927 350 657 4274 1369

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 8486.64 7573.07 12410.93 12290.21 8824.60 8190.81 11290.08 11010.38 8669.14 7785.57 11801.20 11423.59
Standard Deviation 2640.33 2847.62 3070.04 4801.14 2937.11 2527.82 2970.95 3378.86 2809.55 2757.48 3067.76 3940.95
Intensity Change Log 2 — -0.164315 0.548346 0.534244 — -0.107525 0.355453 0.319261 — -0.135086 0.453239 0.428657

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000167 0.000152 0.000085 0.000745 0.000173 0.000048 0.000125 0.000270 0.000170 0.000116 0.000107 0.000424
Bud Neck 0.003935 0.000287 0.000759 0.001295 0.000398 0.000391 0.001008 0.001158 0.002025 0.000323 0.000895 0.001202
Bud Site 0.005353 0.000146 0.000280 0.003321 0.001463 0.000131 0.000686 0.001411 0.003252 0.000141 0.000501 0.002028
Cell Periphery 0.000856 0.001184 0.001523 0.002168 0.001285 0.000619 0.004285 0.001416 0.001088 0.000990 0.003025 0.001659
Cytoplasm 0.000251 0.000051 0.000029 0.000400 0.000383 0.000035 0.000096 0.000864 0.000323 0.000045 0.000066 0.000714
Cytoplasmic Foci 0.007714 0.002082 0.001103 0.006263 0.001890 0.005703 0.001657 0.004167 0.004569 0.003327 0.001404 0.004844
Eisosomes 0.000047 0.000063 0.000480 0.000176 0.000051 0.000046 0.000430 0.000105 0.000049 0.000057 0.000453 0.000128
Endoplasmic Reticulum 0.000134 0.000071 0.000100 0.000139 0.000091 0.000014 0.000296 0.000132 0.000110 0.000051 0.000207 0.000134
Endosome 0.001459 0.000197 0.000067 0.000898 0.000161 0.000063 0.000290 0.000768 0.000758 0.000151 0.000188 0.000810
Golgi 0.009116 0.003856 0.000241 0.003881 0.004680 0.002532 0.000619 0.004002 0.006721 0.003401 0.000447 0.003963
Lipid Particles 0.001160 0.001816 0.001781 0.004256 0.001808 0.000567 0.004011 0.006231 0.001510 0.001386 0.002994 0.005593
Mitochondria 0.002390 0.002231 0.001399 0.019198 0.007710 0.001909 0.004232 0.007101 0.005262 0.002120 0.002940 0.011007
Mitotic Spindle 0.000438 0.000500 0.000065 0.000461 0.001971 0.000162 0.000035 0.000249 0.001266 0.000384 0.000049 0.000317
None 0.000368 0.000556 0.000332 0.001282 0.001696 0.000253 0.000512 0.000882 0.001085 0.000452 0.000430 0.001011
Nuclear Periphery 0.001836 0.001126 0.000619 0.006282 0.002999 0.000399 0.001121 0.002594 0.002464 0.000876 0.000892 0.003785
Nuclear Periphery Foci 0.001369 0.000561 0.000317 0.002193 0.001142 0.000411 0.000591 0.001684 0.001247 0.000509 0.000466 0.001848
Nucleolus 0.015847 0.002929 0.015749 0.006203 0.010486 0.005239 0.008613 0.005842 0.012952 0.003724 0.011867 0.005959
Nucleus 0.001917 0.000436 0.001950 0.002842 0.004829 0.000206 0.001445 0.001240 0.003489 0.000357 0.001675 0.001757
Peroxisomes 0.000343 0.000097 0.000158 0.000231 0.000936 0.000541 0.000144 0.000154 0.000663 0.000250 0.000150 0.000179
Vacuole 0.198609 0.133796 0.355677* 0.250901* 0.056011 0.154677 0.419447* 0.280849* 0.121606 0.140979 0.390367* 0.271180*
Vacuole Periphery 0.746692* 0.847862* 0.617285* 0.686864* 0.899839* 0.826054* 0.550357* 0.678881* 0.829391* 0.840360* 0.580877* 0.681458*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.25 2.29 -2.28 -2.30 -2.60 1.81 1.05 -0.27 -2.93 -1.89 1.23 1.67 -2.02 -2.92 -3.26
Bud Neck 2.30 2.01 1.63 -4.33 -2.46 0.73 -1.96 -2.26 -3.18 -0.70 2.41 1.67 1.24 -5.67 -2.12
Bud Site 1.60 1.57 0.60 -1.55 -1.48 0.99 0.54 -0.00 -2.62 -1.40 1.86 1.65 0.72 -2.56 -2.05
Cell Periphery -1.02 -2.50 -1.40 -1.05 -0.71 0.80 -3.25 -0.17 -3.47 6.05 0.21 -3.76 -1.05 -1.84 3.27
Cytoplasm 2.57 2.93 -1.26 -2.70 -2.86 1.36 1.24 0.10 -1.43 -1.29 1.60 1.51 -0.20 -1.75 -1.67
Cytoplasmic Foci 2.71 3.28 0.14 -2.33 -2.83 -1.28 0.49 -2.14 0.47 -2.79 0.93 3.21 -0.46 -1.41 -4.06
Eisosomes -0.69 -6.73 -2.84 -2.44 3.84 0.32 -8.84 -2.43 -2.57 6.99 -0.53 -10.78 -3.73 -3.11 7.73
Endoplasmic Reticulum 0.97 0.67 -0.10 -1.31 -1.14 1.55 -1.28 0.34 -6.12 5.81 1.58 -0.90 0.28 -2.65 3.36
Endosome 1.18 1.31 0.49 -1.71 -2.08 1.43 -1.63 -2.26 -2.74 -1.87 1.24 1.16 -0.12 -2.95 -2.87
Golgi 1.83 4.12 2.28 -0.02 -4.30 1.19 2.77 0.60 -1.03 -6.74 1.87 4.92 2.13 -0.41 -8.09
Lipid Particles -0.86 -1.95 -5.60 -2.96 -5.31 1.66 -2.90 -2.92 -4.23 -1.69 0.21 -3.40 -4.06 -4.13 -2.86
Mitochondria 0.25 1.64 -4.97 -5.04 -5.34 3.25 1.96 0.25 -3.97 -2.28 3.04 2.32 -3.64 -6.40 -5.94
Mitotic Spindle -0.09 1.04 -0.33 -0.16 -1.84 0.99 1.05 0.96 -0.38 -1.09 0.90 1.16 0.94 0.06 -1.89
None -1.61 0.48 -4.72 -3.86 -5.37 5.82 4.82 2.92 -4.45 -2.68 4.22 4.61 0.37 -4.73 -5.37
Nuclear Periphery 1.06 3.10 -2.94 -3.30 -3.87 2.65 1.98 0.53 -3.06 -2.03 2.45 2.79 -1.27 -3.79 -4.25
Nuclear Periphery Foci 2.16 3.12 -1.78 -4.57 -5.92 2.11 1.77 -0.49 -3.25 -2.89 2.81 3.16 -1.16 -4.49 -5.00
Nucleolus 2.37 0.05 1.71 -2.61 5.22 0.99 0.44 0.92 -0.34 3.46 2.40 0.37 1.83 -2.68 6.73
Nucleus 1.97 0.03 -0.95 -2.25 -1.10 1.83 1.63 1.65 -2.13 0.33 2.02 1.62 1.52 -2.86 -0.50
Peroxisomes 1.46 1.15 0.59 -1.97 -1.50 0.54 1.30 1.28 0.97 -0.45 1.13 1.52 1.42 0.46 -1.33
Vacuole 4.20 -10.50 -3.09 -9.72 9.60 -7.31 -46.47* -25.19 -9.04 15.91 -1.84 -30.14 -15.39 -15.21 17.46
Vacuole Periphery -5.18 6.67 2.81 11.29 -5.15 3.21 22.54 13.24 9.26 -13.44 -1.26 19.27 10.59 16.19 -13.02
Standard name
Human Ortholog
Description Subunit A of the V1 peripheral membrane domain of V-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits; involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Vma1

Vma1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vma1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available