Standard name
Human Ortholog
Description Catalytic subunit of protein phosphatase 2A, PP2A; functionally redundant with Pph21p; C-terminally methylated; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; dephosphorylates Tel1p/Mec1p-phosphorylated Cdc13p to promote telomerase release from telomeres at G2/M; PPH22 has a paralog, PPH21, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.91 0.92 0.86 0.87 0.8 0.73 0.75 0.65 0.7 0.64 0.7 0.9 0.94 0.96 0.53 0.55 0.62 0.88 0.82 0.83 0.79 0.63 0.45
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.14 0.12 0 0 0 0 0.08 0.14
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.15 0.22 0.17 0.35 0.21 0.47 0.17 0 0 0 0 0 0 0 0 0 0 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.06 0 0 0 0.06 0 0 0.05 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0 0 0 0 0.08 0.14
Vac/Vac Membrane 0.12 0.08 0.08 0.18 0.13 0.14 0.14 0.16 0.15 0.14 0.08 0.14 0.13 0.17 0.09 0.37 0.43 0.34 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 2 0 1 0 2 0 0 0 0 1 0 6 1 1 2 4 12
Bud 2 0 1 2 0 2 9 10 8 7 8 3 0 0 0 0 0 0 7 3 2 2 9 21
Bud Neck 0 0 2 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 3
Bud Site 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 0 0 0 0 2 5 5 8 3 6 0 0 2 2 1 0 1 0 0 0 0 0
Cytoplasm 673 171 305 363 510 462 487 483 379 430 222 294 233 286 315 91 113 136 656 155 298 181 235 185
Endoplasmic Reticulum 1 2 3 2 1 0 0 1 2 3 0 4 2 0 0 4 5 3 2 1 3 3 4 2
Endosome 5 1 2 10 6 2 0 0 1 2 0 0 5 2 2 25 29 26 8 1 4 6 28 59
Golgi 1 0 1 0 0 0 0 0 0 0 0 0 1 1 0 3 1 0 4 0 1 1 6 11
Mitochondria 13 0 2 11 29 90 148 112 207 128 161 73 0 1 0 3 5 5 3 2 13 5 10 19
Nucleus 4 3 8 8 6 18 20 25 15 23 14 24 2 0 1 10 5 4 20 11 8 9 16 15
Nuclear Periphery 0 1 0 0 1 0 0 0 0 1 1 1 0 0 0 1 1 1 2 1 1 0 2 1
Nucleolus 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 6 8 8 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 5
SpindlePole 1 0 1 1 0 1 0 0 0 0 0 0 1 0 0 9 16 8 13 1 4 4 29 56
Vac/Vac Membrane 89 15 27 76 78 80 96 101 85 89 29 58 34 50 31 64 88 75 9 4 15 6 12 11
Unique Cell Count 733 188 332 423 583 581 668 646 584 617 345 423 259 303 329 173 207 218 744 190 360 230 373 413
Labelled Cell Count 791 193 353 474 632 656 763 740 702 692 438 465 278 340 351 218 273 267 744 190 360 230 373 413


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 7.3 6.6 6.0 6.0 5.3 5.4 6.0 5.3 5.7 5.0 5.7 6.8 7.4 7.3 8.9 8.8 9.0 6.7 6.6 7.7
Std Deviation (1e-4) 1.0 1.6 1.2 1.6 1.5 1.4 1.4 1.4 1.4 1.0 1.3 1.1 1.6 2.1 1.7 2.5 1.7 2.3 1.8 1.9 2.7
Intensity Change (Log2) – – – -0.13 -0.14 -0.3 -0.28 -0.14 -0.3 -0.22 -0.41 -0.21 0.04 0.16 0.15 0.43 0.42 0.45 0.02 0.0 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.6 -2.0 -4.9 -7.0 -6.4 -9.0 -7.8 -8.6 -7.5 -0.8 1.2 2.1 -10.2 -10.1 -8.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 1.9 0 0 0 0 0 0 0 0 0 0 0 6.6 6.5 5.9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.1 3.5 7.2 9.0 7.7 12.1 8.6 14.0 7.6 0 0 0 0 0 0
Nucleus -0.5 -1.6 0.6 0.5 1.2 0.1 1.1 1.2 2.2 0 0 0 1.9 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 4.8 0
Vacuole 3.9 2.4 2.5 2.8 3.3 2.9 2.8 0.1 2.4 2.0 3.2 0.6 8.0 9.5 7.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5962 2.9678 2.767 2.2605 2.2169 2.8108 1.696 1.9894 2.0008 2.4982 1.8029 1.8832 2.4267 2.0337 2.2378 1.9698 2.1389 1.7919
Actin 0.0183 0.0012 0.0031 0.0011 0.0054 0.0064 0.0159 0.0009 0.0149 0.0225 0.0201 0.0014 0.0399 0.0018 0.0078 0.0435 0.0144 0.0008
Bud 0.0006 0.0008 0.0012 0.0019 0.0002 0.0005 0.001 0.0006 0.0005 0.0038 0.003 0.0017 0.001 0.0054 0.0035 0.001 0.0026 0.0034
Bud Neck 0.0104 0.0007 0.0016 0.0014 0.002 0.0073 0.0064 0.0016 0.0046 0.0262 0.0043 0.0036 0.0086 0.0004 0.0056 0.0029 0.0011 0.0024
Bud Periphery 0.0014 0.0007 0.0017 0.0053 0.0001 0.0005 0.0011 0.0003 0.0005 0.0043 0.0048 0.001 0.0011 0.006 0.0011 0.0007 0.0049 0.0022
Bud Site 0.0057 0.0038 0.0034 0.0012 0.0003 0.0005 0.0064 0.0104 0.0031 0.0509 0.0095 0.0005 0.0073 0.0078 0.0022 0.0014 0.0036 0.0004
Cell Periphery 0.0006 0.0001 0.0001 0.0001 0 0.0001 0.0002 0.0001 0.0002 0.0007 0.0001 0.0001 0.0004 0.0002 0.0001 0.0002 0.0002 0.0001
Cytoplasm 0.5112 0.6511 0.5117 0.6303 0.5954 0.5997 0.5713 0.5921 0.5606 0.513 0.4805 0.6112 0.5692 0.7962 0.6883 0.6609 0.5735 0.7265
Cytoplasmic Foci 0.0181 0.0108 0.0178 0.0097 0.0111 0.0159 0.0189 0.0087 0.0124 0.0289 0.0225 0.0136 0.0254 0.0087 0.0097 0.0125 0.0344 0.0087
Eisosomes 0.0005 0 0 0 0.0001 0.0002 0.0001 0 0.0002 0.0006 0.0001 0 0.0003 0 0.0001 0.0008 0.0002 0
Endoplasmic Reticulum 0.006 0.0033 0.0037 0.0046 0.0039 0.0046 0.0044 0.0023 0.0033 0.0082 0.0054 0.0021 0.0063 0.0033 0.0062 0.0043 0.0055 0.0026
Endosome 0.0145 0.0059 0.0086 0.0058 0.0095 0.014 0.0132 0.0031 0.0096 0.0242 0.0195 0.0049 0.0344 0.0028 0.0108 0.0301 0.0543 0.0041
Golgi 0.0027 0.0003 0.0013 0.0003 0.0007 0.0013 0.0027 0.0003 0.0028 0.0044 0.0049 0.0011 0.01 0.0004 0.0017 0.0096 0.0118 0.0003
Lipid Particles 0.0082 0.0002 0.001 0.0003 0.0009 0.0009 0.004 0.0001 0.0027 0.0079 0.007 0.0003 0.0173 0.0007 0.001 0.0108 0.0095 0.0001
Mitochondria 0.0057 0.0006 0.0011 0.0022 0.0007 0.0012 0.0041 0.0006 0.0056 0.0024 0.0012 0.0014 0.0059 0.0007 0.0102 0.0108 0.0119 0.0014
None 0.2865 0.209 0.3541 0.268 0.1849 0.2301 0.1969 0.297 0.2307 0.1066 0.3201 0.182 0.1573 0.091 0.1551 0.0772 0.1648 0.1239
Nuclear Periphery 0.0104 0.0056 0.0061 0.007 0.0099 0.0086 0.0082 0.0036 0.0078 0.0197 0.0175 0.0059 0.0122 0.0036 0.0158 0.0065 0.0311 0.0046
Nucleolus 0.0011 0.0005 0.0007 0.0004 0.0008 0.001 0.001 0.0008 0.0015 0.0047 0.0008 0.0009 0.0038 0.0007 0.0009 0.0004 0.0016 0.0003
Nucleus 0.078 0.0965 0.0617 0.0503 0.1397 0.0916 0.1146 0.0669 0.1146 0.072 0.0453 0.1368 0.0615 0.0628 0.0673 0.0771 0.0459 0.1104
Peroxisomes 0.009 0.0003 0.0074 0.0008 0.0007 0.0008 0.0139 0.0005 0.0097 0.0149 0.0057 0.0019 0.0149 0.0006 0.0006 0.0397 0.0102 0.0005
Punctate Nuclear 0.0065 0.0062 0.0112 0.0075 0.0318 0.0123 0.0127 0.0084 0.0127 0.081 0.0256 0.0279 0.0144 0.0048 0.0075 0.0035 0.0097 0.0057
Vacuole 0.0038 0.0021 0.0019 0.0014 0.0013 0.0018 0.0026 0.0016 0.0015 0.0022 0.0015 0.0014 0.0063 0.002 0.0034 0.0036 0.005 0.0014
Vacuole Periphery 0.0008 0.0003 0.0004 0.0004 0.0003 0.0006 0.0006 0.0002 0.0005 0.0008 0.0005 0.0003 0.0024 0.0003 0.001 0.0023 0.0038 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.0296 44.3608 41.9539 70.6331 56.1395 57.3764 58.0453 63.2169 69.5607 63.4217
Translational Efficiency 1.1744 1.2224 1.4098 0.9739 1.0601 1.2101 1.1561 1.0379 0.9675 0.9399

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Catalytic subunit of protein phosphatase 2A, PP2A; functionally redundant with Pph21p; C-terminally methylated; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; dephosphorylates Tel1p/Mec1p-phosphorylated Cdc13p to promote telomerase release from telomeres at G2/M; PPH22 has a paralog, PPH21, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (41%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pph22

Pph22


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pph22-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available