Standard name | |
---|---|
Human Ortholog | |
Description | Component of the Sec13p-Sec31p complex of the COPII vesicle coat; COPII coat is required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 131 | 112 | 237 | 192 | 146 | 194 | 200 | 121 | 121 | – | 66 | 101 | 88 | 102 | 137 | 114 | 59 | 74 | 170 | 95 | 243 | 71 | 146 | 161 |
Bud | 4 | 4 | 21 | 22 | 23 | 25 | 26 | 18 | 19 | – | 23 | 24 | 6 | 7 | 15 | 7 | 2 | 11 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 10 | 7 | 20 | 21 | 18 | 19 | 11 | 3 | 10 | – | 7 | 16 | 5 | 3 | 3 | 6 | 8 | 12 | 0 | 0 | 0 | 0 | 2 | 3 |
Cytoplasm | 13 | 19 | 10 | 9 | 17 | 15 | 30 | 24 | 12 | – | 6 | 10 | 10 | 6 | 11 | 25 | 20 | 37 | 2 | 2 | 0 | 0 | 2 | 0 |
Endoplasmic Reticulum | 209 | 138 | 275 | 116 | 124 | 157 | 221 | 198 | 112 | – | 45 | 60 | 104 | 113 | 99 | 16 | 5 | 17 | 66 | 57 | 42 | 16 | 35 | 16 |
Endosome | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 2 | – | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 1 | 0 | 0 |
Golgi | 328 | 124 | 263 | 181 | 235 | 204 | 262 | 190 | 80 | – | 56 | 60 | 128 | 183 | 159 | 254 | 159 | 198 | 139 | 64 | 115 | 45 | 56 | 46 |
Mitochondria | 42 | 18 | 118 | 194 | 153 | 230 | 311 | 382 | 351 | – | 256 | 377 | 32 | 51 | 126 | 15 | 25 | 16 | 4 | 3 | 0 | 0 | 1 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 1 | 0 | 2 | 3 | 8 | 7 | 4 | – | 5 | 11 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 1 | – | 0 | 1 | 0 | 0 | 2 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 1 | 1 | 5 | 1 | 0 | – | 3 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 2 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 9 | 1 | 1 | 1 | 0 | 1 | 1 | 6 | 0 | – | 0 | 0 | 0 | 0 | 1 | 6 | 1 | 3 | 0 | 2 | 0 | 1 | 0 | 0 |
Unique Cell Count | 378 | 226 | 418 | 309 | 332 | 390 | 546 | 552 | 410 | 286 | 417 | 182 | 231 | 268 | 288 | 181 | 253 | 390 | 231 | 413 | 140 | 248 | 235 | |
Labelled Cell Count | 748 | 423 | 947 | 738 | 721 | 849 | 1077 | 954 | 712 | 468 | 665 | 374 | 465 | 557 | 445 | 281 | 371 | 390 | 231 | 413 | 140 | 248 | 235 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 36.3 | 33.4 | 22.4 | 21.9 | 22.1 | 20.4 | 19.8 | 18.4 | 17.1 | – | 15.9 | 15.3 | 29.6 | 29.1 | 26.9 | 33.3 | 36.4 | 39.1 | 29.2 | 32.3 | 31.9 |
Std Deviation (1e-4) | 9.1 | 6.9 | 3.9 | 4.7 | 5.0 | 4.0 | 4.4 | 4.2 | 4.2 | – | 3.7 | 3.7 | 6.9 | 7.5 | 7.4 | 11.8 | 12.9 | 14.5 | 6.3 | 7.9 | 8.4 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.02 | -0.13 | -0.18 | -0.28 | -0.39 | – | -0.49 | -0.55 | 0.4 | 0.38 | 0.26 | 0.57 | 0.7 | 0.8 | 0.38 | 0.52 | 0.51 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.5 | -3.5 | -2.0 | -6.2 | -11.1 | -7.9 | – | -8.8 | -9.6 | -1.9 | -3.1 | -1.4 | -4.5 | -5.4 | -6.9 |
Bud | 1.2 | 1.1 | 0.8 | -0.2 | -1.4 | -0.3 | – | 1.6 | 0.5 | -0.9 | -1.2 | 0.3 | -1.7 | -2.3 | -0.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.2 | 0.4 | 0.1 | -2.4 | -4.3 | -1.8 | – | -1.6 | -0.7 | -1.1 | -2.3 | -2.6 | -1.9 | -0.2 | 0 |
Cytoplasm | 0.4 | 2.0 | 1.2 | 2.4 | 1.6 | 0.5 | – | -0.3 | 0 | 1.9 | 0.2 | 1.3 | 3.8 | 4.5 | 6.0 |
Endoplasmic Reticulum | -7.6 | -7.8 | -7.3 | -7.8 | -9.2 | -11.1 | – | -13.1 | -15.2 | -2.0 | -4.2 | -7.4 | -16.0 | -14.2 | -15.0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -1.2 | 2.3 | -3.1 | -4.6 | -8.8 | -12.7 | – | -11.3 | -14.4 | 1.8 | 4.3 | -0.9 | 7.5 | 6.1 | 4.2 |
Mitochondria | 9.3 | 5.1 | 8.8 | 8.9 | 12.6 | 16.7 | – | 16.0 | 18.3 | -2.8 | -1.7 | 5.0 | -7.7 | -3.8 | -6.9 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 39.6986 | 47.6429 | 43.9242 | 47.6415 | 41.6402 | 47.3913 | 33.2173 | 44.5992 | 41.7452 | 39.8984 | 41.9118 | 41.1318 | 31.0069 | 39.8802 | 39.9198 | 37.8099 | 33.1209 | 36.3534 |
Actin | 0.1774 | 0.0943 | 0.0845 | 0.0064 | 0.0458 | 0.0341 | 0.1339 | 0.068 | 0.0628 | 0.0394 | 0.0113 | 0.0327 | 0.1888 | 0.1687 | 0.2098 | 0.3256 | 0.0424 | 0.1237 |
Bud | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0006 | 0.0007 | 0.0002 | 0 | 0 | 0.0002 | 0 |
Bud Neck | 0.001 | 0.0003 | 0.0008 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.0006 | 0.0009 | 0.0002 | 0.0002 | 0.0002 | 0.0005 | 0.0006 | 0.0005 | 0.0008 | 0.0003 | 0.0003 |
Bud Periphery | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0 | 0.0004 | 0.0011 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0001 |
Bud Site | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0.0026 | 0.0002 | 0.0003 | 0.002 | 0 | 0.0006 | 0.0029 | 0.0004 | 0.0001 | 0 | 0.0005 | 0.0001 |
Cell Periphery | 0.0009 | 0.0004 | 0.0007 | 0.0004 | 0.0002 | 0.0004 | 0.0006 | 0.0009 | 0.0005 | 0.0007 | 0.0007 | 0.0004 | 0.0005 | 0.0006 | 0.0005 | 0.0004 | 0.0004 | 0.0002 |
Cytoplasm | 0.0001 | 0.0004 | 0.0001 | 0 | 0 | 0 | 0.0036 | 0.0023 | 0.0029 | 0.0036 | 0 | 0.004 | 0.0021 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0012 |
Cytoplasmic Foci | 0.002 | 0.0024 | 0.0012 | 0.0005 | 0.0008 | 0.0005 | 0.0105 | 0.0029 | 0.0013 | 0.0033 | 0.0008 | 0.0012 | 0.0144 | 0.0022 | 0.0011 | 0.001 | 0.0146 | 0.0034 |
Eisosomes | 0.0597 | 0.0428 | 0.0459 | 0.0212 | 0.0519 | 0.0173 | 0.0641 | 0.0245 | 0.0552 | 0.0432 | 0.0459 | 0.0228 | 0.0393 | 0.0168 | 0.039 | 0.1851 | 0.0731 | 0.0098 |
Endoplasmic Reticulum | 0.0016 | 0.0021 | 0.0024 | 0.0013 | 0.001 | 0.0023 | 0.0053 | 0.0038 | 0.003 | 0.0056 | 0.0032 | 0.0035 | 0.0116 | 0.0012 | 0.0017 | 0.0017 | 0.0014 | 0.0053 |
Endosome | 0.0079 | 0.0057 | 0.0053 | 0.0027 | 0.0029 | 0.0034 | 0.0272 | 0.0091 | 0.0091 | 0.0074 | 0.003 | 0.0092 | 0.0238 | 0.0037 | 0.0026 | 0.0051 | 0.005 | 0.0094 |
Golgi | 0.7168 | 0.8453 | 0.8459 | 0.9625 | 0.8926 | 0.9279 | 0.6879 | 0.874 | 0.842 | 0.8731 | 0.9211 | 0.9177 | 0.6148 | 0.7567 | 0.7387 | 0.4747 | 0.7721 | 0.8365 |
Lipid Particles | 0.0206 | 0.0034 | 0.0094 | 0.0038 | 0.0039 | 0.002 | 0.0224 | 0.0072 | 0.0039 | 0.0125 | 0.0127 | 0.003 | 0.016 | 0.0121 | 0.0043 | 0.003 | 0.0142 | 0.0035 |
Mitochondria | 0.009 | 0.0011 | 0.0015 | 0.0004 | 0.0004 | 0.0099 | 0.0266 | 0.0039 | 0.0028 | 0.0035 | 0.0004 | 0.0018 | 0.0371 | 0.0147 | 0.001 | 0.0016 | 0.0581 | 0.0044 |
None | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0009 | 0.0001 | 0.008 | 0.001 | 0 | 0.0002 | 0.0069 | 0.0003 | 0 | 0 | 0.0013 | 0.0001 |
Nuclear Periphery | 0.0005 | 0.0002 | 0.0007 | 0.0003 | 0.0001 | 0.0006 | 0.0041 | 0.0009 | 0.0027 | 0.0015 | 0.0002 | 0.0005 | 0.0095 | 0.0028 | 0.0002 | 0.0001 | 0.0002 | 0.0009 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0.0011 | 0 | 0.0001 | 0 | 0 | 0 | 0.0007 | 0.0057 | 0 | 0 | 0.0029 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0 | 0.0009 | 0.0002 | 0 | 0.0003 | 0.0013 | 0.0105 | 0 | 0 | 0.0006 | 0.0001 |
Peroxisomes | 0.0004 | 0.0006 | 0 | 0 | 0 | 0 | 0.0017 | 0.0004 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0.0034 | 0.0004 | 0.0001 | 0.0001 | 0.0103 | 0.0004 |
Punctate Nuclear | 0 | 0 | 0 | 0 | 0 | 0 | 0.0008 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0214 | 0.0012 | 0 | 0 | 0.0004 | 0 |
Vacuole | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0017 | 0.0003 | 0.0021 | 0.0003 | 0 | 0.0002 | 0.0013 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.0002 |
Vacuole Periphery | 0.0012 | 0.0007 | 0.0013 | 0.0004 | 0.0002 | 0.0013 | 0.0025 | 0.0006 | 0.0011 | 0.0009 | 0.0003 | 0.0005 | 0.0019 | 0.0007 | 0.0002 | 0.0003 | 0.001 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 147.9578 | 142.7174 | 113.3712 | 136.6231 | 129.8855 | 120.3205 | 133.3418 | 116.4146 | 115.973 | 149.8794 |
Translational Efficiency | 1.3922 | 1.3061 | 1.3091 | 1.0282 | 1.2094 | 1.5319 | 1.1107 | 1.0361 | 1.2065 | 1.1244 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1848 | 125 | 191 | 1239 | 1687 | 1857 | 1623 | 2873 | 3535 | 1982 | 1814 | 4112 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1907.02 | 2333.64 | 3447.05 | 1782.54 | 1557.01 | 1649.04 | 3238.40 | 2301.43 | 1739.99 | 1692.22 | 3260.37 | 2145.08 |
Standard Deviation | 826.44 | 598.93 | 1673.12 | 1205.47 | 622.35 | 848.31 | 780.58 | 1415.26 | 756.61 | 851.22 | 918.69 | 1376.22 |
Intensity Change Log 2 | — | 0.291262 | 0.854042 | -0.097386 | — | 0.082848 | 1.056503 | 0.563752 | — | 0.201288 | 0.948573 | 0.237521 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.153858* | 0.175965* | 0.116673* | 0.104489* | 0.298538* | 0.291269* | 0.090835 | 0.226227* | 0.222903* | 0.283997* | 0.093555 | 0.189546* |
Bud Neck | 0.002638 | 0.005237 | 0.033411 | 0.006664 | 0.003539 | 0.007303 | 0.003912 | 0.004454 | 0.003068 | 0.007173 | 0.007018 | 0.005120 |
Bud Site | 0.029877 | 0.010937 | 0.018813 | 0.076229 | 0.026666 | 0.066753 | 0.007732 | 0.080615 | 0.028345 | 0.063233 | 0.008899 | 0.079294 |
Cell Periphery | 0.002507 | 0.001181 | 0.013014 | 0.007811 | 0.001595 | 0.001641 | 0.004269 | 0.007890 | 0.002072 | 0.001612 | 0.005190 | 0.007866 |
Cytoplasm | 0.063473 | 0.020629 | 0.025639 | 0.132744* | 0.040644 | 0.120800* | 0.004849 | 0.097490 | 0.052578 | 0.114482* | 0.007038 | 0.108112* |
Cytoplasmic Foci | 0.011555 | 0.021437 | 0.009273 | 0.009617 | 0.011866 | 0.036907 | 0.000885 | 0.004515 | 0.011704 | 0.035931 | 0.001769 | 0.006053 |
Eisosomes | 0.116328* | 0.300593* | 0.106333* | 0.070864 | 0.236446* | 0.108450* | 0.169327* | 0.072090 | 0.173652* | 0.120568* | 0.162694* | 0.071721 |
Endoplasmic Reticulum | 0.083058 | 0.088030 | 0.185294* | 0.129224* | 0.039803 | 0.061026 | 0.207977* | 0.131723* | 0.062416 | 0.062729 | 0.205588* | 0.130970* |
Endosome | 0.008647 | 0.007931 | 0.016471 | 0.021316 | 0.005495 | 0.012883 | 0.011983 | 0.019756 | 0.007143 | 0.012571 | 0.012455 | 0.020226 |
Golgi | 0.398184* | 0.294884* | 0.184996* | 0.175307* | 0.240089* | 0.189550* | 0.377612* | 0.187224* | 0.322737* | 0.196193* | 0.357331* | 0.183633* |
Lipid Particles | 0.093549 | 0.059023 | 0.127802* | 0.157366* | 0.068331 | 0.063232 | 0.109299* | 0.116717* | 0.081514 | 0.062966 | 0.111247* | 0.128965* |
Mitochondria | 0.007163 | 0.001911 | 0.004644 | 0.008793 | 0.004848 | 0.005317 | 0.001939 | 0.017584 | 0.006059 | 0.005102 | 0.002224 | 0.014936 |
Mitotic Spindle | 0.000674 | 0.000380 | 0.003232 | 0.021142 | 0.001049 | 0.005225 | 0.000511 | 0.010945 | 0.000853 | 0.004920 | 0.000797 | 0.014017 |
None | 0.015896 | 0.001684 | 0.000997 | 0.003767 | 0.009058 | 0.002494 | 0.000545 | 0.003808 | 0.012633 | 0.002443 | 0.000592 | 0.003795 |
Nuclear Periphery | 0.000207 | 0.000250 | 0.006011 | 0.000885 | 0.000195 | 0.000203 | 0.000806 | 0.000479 | 0.000201 | 0.000206 | 0.001354 | 0.000601 |
Nuclear Periphery Foci | 0.000347 | 0.000194 | 0.001967 | 0.000763 | 0.000308 | 0.000285 | 0.000159 | 0.000283 | 0.000329 | 0.000279 | 0.000349 | 0.000428 |
Nucleolus | 0.000254 | 0.000148 | 0.000618 | 0.000454 | 0.000334 | 0.000315 | 0.000013 | 0.000223 | 0.000292 | 0.000305 | 0.000077 | 0.000293 |
Nucleus | 0.007826 | 0.001400 | 0.078406 | 0.040649 | 0.008346 | 0.013311 | 0.002665 | 0.008672 | 0.008074 | 0.012560 | 0.010640 | 0.018307 |
Peroxisomes | 0.000668 | 0.004163 | 0.000230 | 0.000888 | 0.000745 | 0.002354 | 0.000405 | 0.001619 | 0.000705 | 0.002469 | 0.000386 | 0.001399 |
Vacuole | 0.002136 | 0.003294 | 0.055138 | 0.028260 | 0.001454 | 0.009273 | 0.003016 | 0.006196 | 0.001811 | 0.008896 | 0.008504 | 0.012844 |
Vacuole Periphery | 0.001154 | 0.000730 | 0.011038 | 0.002766 | 0.000652 | 0.001408 | 0.001262 | 0.001489 | 0.000914 | 0.001366 | 0.002291 | 0.001873 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.93 | 3.46 | 8.34 | 4.10 | 1.26 | 0.68 | 20.11* | 7.89 | 7.41 | -15.34 | -6.19 | 17.52 | 6.70 | 11.82 | -12.12 |
Bud Neck | -2.45 | -8.07 | -6.57 | -1.33 | 6.82 | -6.53 | -0.90 | -3.27 | 3.73 | -1.90 | -7.71 | -6.35 | -6.71 | 2.94 | 2.01 |
Bud Site | 2.75 | 0.63 | -9.01 | -8.60 | -6.46 | -9.08 | 6.03 | -14.77 | -4.20 | -21.97* | -9.00 | 7.50 | -17.43 | -4.92 | -24.07 |
Cell Periphery | 3.38 | -6.23 | -5.41 | -7.07 | 3.48 | -0.31 | -7.97 | -15.40 | -15.67 | -6.57 | 2.68 | -8.53 | -14.53 | -16.58 | -4.35 |
Cytoplasm | 4.38 | 2.09 | -14.31 | -14.52 | -13.21 | -12.48 | 9.50 | -15.35 | -1.05 | -25.33 | -10.56 | 13.47 | -19.33 | -4.73 | -32.16 |
Cytoplasmic Foci | -1.03 | -1.13 | -0.52 | 0.95 | 0.96 | -8.86 | 7.51 | 4.50 | 12.83 | -9.00 | -9.52 | 8.40 | 4.16 | 11.95 | -7.54 |
Eisosomes | -6.50 | 1.94 | 9.93 | 8.80 | 3.00 | 14.65 | 7.08 | 21.36 | 7.35 | 14.93 | 8.12 | 1.86 | 21.95 | 10.49 | 15.89 |
Endoplasmic Reticulum | -0.43 | -5.26 | -2.06 | -0.53 | 4.33 | -5.73 | -29.88 | -19.49 | -13.07 | 14.01 | 0.06 | -26.58 | -14.44 | -12.54 | 15.96 |
Endosome | 0.73 | -5.60 | -7.29 | -6.45 | -0.10 | -8.27 | -13.17 | -17.57 | -5.04 | -8.77 | -6.72 | -12.66 | -17.82 | -5.32 | -8.36 |
Golgi | 3.89 | 14.45 | 26.55 | 6.48 | 1.91 | 5.52 | -13.29 | 8.27 | 2.29 | 23.43* | 15.43 | -3.38 | 24.52 | 5.16 | 24.64* |
Lipid Particles | 3.03 | -1.42 | -2.95 | -4.56 | -0.30 | 1.15 | -7.51 | -7.21 | -8.99 | 0.56 | 4.64 | -6.01 | -6.53 | -10.56 | 0.08 |
Mitochondria | 3.88 | 0.88 | -0.83 | -4.38 | -1.49 | -0.52 | 3.44 | -9.04 | -8.42 | -11.33 | 1.11 | 4.88 | -7.90 | -8.37 | -11.68 |
Mitotic Spindle | 1.25 | -2.34 | -9.70 | -9.80 | -5.37 | -3.95 | 0.39 | -10.52 | -5.78 | -10.74 | -4.25 | -1.41 | -14.48 | -8.58 | -13.43 |
None | 6.22 | 7.34 | 5.89 | -1.49 | -3.18 | 4.80 | 6.38 | 3.56 | -2.15 | -5.85 | 8.19 | 9.94 | 6.83 | -2.57 | -7.11 |
Nuclear Periphery | -1.19 | -8.74 | -16.29 | -15.59 | 7.09 | -0.43 | -11.12 | -12.00 | -11.45 | 4.77 | -0.27 | -12.54 | -18.69 | -18.75 | 6.39 |
Nuclear Periphery Foci | 1.23 | -5.15 | -4.25 | -4.17 | 4.10 | 0.38 | 2.39 | 0.36 | -0.05 | -2.93 | 1.05 | -1.40 | -2.20 | -3.59 | -0.22 |
Nucleolus | 0.88 | -2.50 | -2.09 | -2.24 | 1.58 | 0.21 | 4.01 | 0.56 | 0.46 | -2.10 | -0.20 | 3.02 | -0.44 | -0.30 | -2.23 |
Nucleus | 5.14 | -5.98 | -15.55 | -17.03 | 1.57 | -2.53 | 2.80 | -4.10 | -1.62 | -7.21 | -2.91 | -3.07 | -14.50 | -11.28 | -7.47 |
Peroxisomes | -1.16 | 1.83 | -0.23 | 1.14 | -1.25 | -4.79 | 2.62 | -2.09 | 1.96 | -3.09 | -4.83 | 2.56 | -2.09 | 2.86 | -3.26 |
Vacuole | -0.75 | -7.60 | -15.27 | -14.39 | -0.08 | -6.96 | -3.72 | -9.93 | -1.83 | -7.22 | -6.69 | -7.45 | -17.29 | -11.47 | -9.63 |
Vacuole Periphery | 1.28 | -4.96 | -2.32 | -4.02 | 4.37 | -2.31 | -4.97 | -5.39 | -0.06 | -1.17 | -1.34 | -4.80 | -3.63 | -0.89 | 2.14 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|