Standard name
Human Ortholog
Description Component of the Sec13p-Sec31p complex of the COPII vesicle coat; COPII coat is required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.35 0.5 0.57 0.62 0.44 0.5 0.37 0.22 0.3 – 0.23 0.24 0.48 0.44 0.51 0.4 0.33 0.29 0.44 0.41 0.59 0.51 0.59 0.69
Bud 0 0 0.05 0.07 0.07 0.06 0.05 0 0.05 – 0.08 0.06 0 0 0.06 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0.07 0.05 0.05 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0 0.08 0 0 0.05 0 0.05 0 0 – 0 0 0.05 0 0 0.09 0.11 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0.55 0.61 0.66 0.38 0.37 0.4 0.4 0.36 0.27 – 0.16 0.14 0.57 0.49 0.37 0.06 0 0.07 0.17 0.25 0.1 0.12 0.14 0.07
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.87 0.55 0.63 0.59 0.71 0.52 0.48 0.34 0.2 – 0.2 0.14 0.7 0.79 0.59 0.88 0.88 0.78 0.36 0.28 0.28 0.33 0.23 0.2
Mitochondria 0.11 0.08 0.28 0.63 0.46 0.59 0.57 0.69 0.86 – 0.9 0.9 0.18 0.22 0.47 0.05 0.14 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 131 112 237 192 146 194 200 121 121 – 66 101 88 102 137 114 59 74 170 95 243 71 146 161
Bud 4 4 21 22 23 25 26 18 19 – 23 24 6 7 15 7 2 11 1 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 10 7 20 21 18 19 11 3 10 – 7 16 5 3 3 6 8 12 0 0 0 0 2 3
Cytoplasm 13 19 10 9 17 15 30 24 12 – 6 10 10 6 11 25 20 37 2 2 0 0 2 0
Endoplasmic Reticulum 209 138 275 116 124 157 221 198 112 – 45 60 104 113 99 16 5 17 66 57 42 16 35 16
Endosome 0 0 1 0 1 0 1 1 2 – 0 0 1 0 1 1 0 2 0 0 1 1 0 0
Golgi 328 124 263 181 235 204 262 190 80 – 56 60 128 183 159 254 159 198 139 64 115 45 56 46
Mitochondria 42 18 118 194 153 230 311 382 351 – 256 377 32 51 126 15 25 16 4 3 0 0 1 0
Nucleus 0 0 0 0 0 0 1 1 0 – 1 0 0 0 1 0 0 0 1 0 0 0 0 0
Nuclear Periphery 1 0 1 0 2 3 8 7 4 – 5 11 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 1 1 0 0 2 1 – 0 1 0 0 2 1 2 1 0 0 0 0 0 0
Peroxisomes 0 0 0 1 1 1 5 1 0 – 3 4 0 0 1 0 0 0 0 0 4 0 0 2
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 9 1 1 1 0 1 1 6 0 – 0 0 0 0 1 6 1 3 0 2 0 1 0 0
Unique Cell Count 378 226 418 309 332 390 546 552 410 286 417 182 231 268 288 181 253 390 231 413 140 248 235
Labelled Cell Count 748 423 947 738 721 849 1077 954 712 468 665 374 465 557 445 281 371 390 231 413 140 248 235


ER to Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 36.3 33.4 22.4 21.9 22.1 20.4 19.8 18.4 17.1 – 15.9 15.3 29.6 29.1 26.9 33.3 36.4 39.1 29.2 32.3 31.9
Std Deviation (1e-4) 9.1 6.9 3.9 4.7 5.0 4.0 4.4 4.2 4.2 – 3.7 3.7 6.9 7.5 7.4 11.8 12.9 14.5 6.3 7.9 8.4
Intensity Change (Log2) – – – -0.03 -0.02 -0.13 -0.18 -0.28 -0.39 – -0.49 -0.55 0.4 0.38 0.26 0.57 0.7 0.8 0.38 0.52 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.5 -3.5 -2.0 -6.2 -11.1 -7.9 – -8.8 -9.6 -1.9 -3.1 -1.4 -4.5 -5.4 -6.9
Bud 1.2 1.1 0.8 -0.2 -1.4 -0.3 – 1.6 0.5 -0.9 -1.2 0.3 -1.7 -2.3 -0.4
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 1.2 0.4 0.1 -2.4 -4.3 -1.8 – -1.6 -0.7 -1.1 -2.3 -2.6 -1.9 -0.2 0
Cytoplasm 0.4 2.0 1.2 2.4 1.6 0.5 – -0.3 0 1.9 0.2 1.3 3.8 4.5 6.0
Endoplasmic Reticulum -7.6 -7.8 -7.3 -7.8 -9.2 -11.1 – -13.1 -15.2 -2.0 -4.2 -7.4 -16.0 -14.2 -15.0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi -1.2 2.3 -3.1 -4.6 -8.8 -12.7 – -11.3 -14.4 1.8 4.3 -0.9 7.5 6.1 4.2
Mitochondria 9.3 5.1 8.8 8.9 12.6 16.7 – 16.0 18.3 -2.8 -1.7 5.0 -7.7 -3.8 -6.9
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 2.9 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 39.6986 47.6429 43.9242 47.6415 41.6402 47.3913 33.2173 44.5992 41.7452 39.8984 41.9118 41.1318 31.0069 39.8802 39.9198 37.8099 33.1209 36.3534
Actin 0.1774 0.0943 0.0845 0.0064 0.0458 0.0341 0.1339 0.068 0.0628 0.0394 0.0113 0.0327 0.1888 0.1687 0.2098 0.3256 0.0424 0.1237
Bud 0.0001 0 0 0 0 0 0.0005 0.0001 0.0001 0.0004 0 0.0006 0.0007 0.0002 0 0 0.0002 0
Bud Neck 0.001 0.0003 0.0008 0.0001 0.0002 0.0002 0.0006 0.0006 0.0009 0.0002 0.0002 0.0002 0.0005 0.0006 0.0005 0.0008 0.0003 0.0003
Bud Periphery 0.0002 0 0 0 0 0 0.0005 0.0001 0.0002 0.0003 0 0.0004 0.0011 0.0002 0 0.0001 0.0002 0.0001
Bud Site 0.0003 0.0001 0 0 0 0 0.0026 0.0002 0.0003 0.002 0 0.0006 0.0029 0.0004 0.0001 0 0.0005 0.0001
Cell Periphery 0.0009 0.0004 0.0007 0.0004 0.0002 0.0004 0.0006 0.0009 0.0005 0.0007 0.0007 0.0004 0.0005 0.0006 0.0005 0.0004 0.0004 0.0002
Cytoplasm 0.0001 0.0004 0.0001 0 0 0 0.0036 0.0023 0.0029 0.0036 0 0.004 0.0021 0.0002 0.0001 0.0001 0.0003 0.0012
Cytoplasmic Foci 0.002 0.0024 0.0012 0.0005 0.0008 0.0005 0.0105 0.0029 0.0013 0.0033 0.0008 0.0012 0.0144 0.0022 0.0011 0.001 0.0146 0.0034
Eisosomes 0.0597 0.0428 0.0459 0.0212 0.0519 0.0173 0.0641 0.0245 0.0552 0.0432 0.0459 0.0228 0.0393 0.0168 0.039 0.1851 0.0731 0.0098
Endoplasmic Reticulum 0.0016 0.0021 0.0024 0.0013 0.001 0.0023 0.0053 0.0038 0.003 0.0056 0.0032 0.0035 0.0116 0.0012 0.0017 0.0017 0.0014 0.0053
Endosome 0.0079 0.0057 0.0053 0.0027 0.0029 0.0034 0.0272 0.0091 0.0091 0.0074 0.003 0.0092 0.0238 0.0037 0.0026 0.0051 0.005 0.0094
Golgi 0.7168 0.8453 0.8459 0.9625 0.8926 0.9279 0.6879 0.874 0.842 0.8731 0.9211 0.9177 0.6148 0.7567 0.7387 0.4747 0.7721 0.8365
Lipid Particles 0.0206 0.0034 0.0094 0.0038 0.0039 0.002 0.0224 0.0072 0.0039 0.0125 0.0127 0.003 0.016 0.0121 0.0043 0.003 0.0142 0.0035
Mitochondria 0.009 0.0011 0.0015 0.0004 0.0004 0.0099 0.0266 0.0039 0.0028 0.0035 0.0004 0.0018 0.0371 0.0147 0.001 0.0016 0.0581 0.0044
None 0.0001 0 0 0 0 0 0.0009 0.0001 0.008 0.001 0 0.0002 0.0069 0.0003 0 0 0.0013 0.0001
Nuclear Periphery 0.0005 0.0002 0.0007 0.0003 0.0001 0.0006 0.0041 0.0009 0.0027 0.0015 0.0002 0.0005 0.0095 0.0028 0.0002 0.0001 0.0002 0.0009
Nucleolus 0 0 0 0 0 0 0.0011 0 0.0001 0 0 0 0.0007 0.0057 0 0 0.0029 0
Nucleus 0 0 0 0 0 0 0.0008 0 0.0009 0.0002 0 0.0003 0.0013 0.0105 0 0 0.0006 0.0001
Peroxisomes 0.0004 0.0006 0 0 0 0 0.0017 0.0004 0.0001 0.0005 0.0001 0.0001 0.0034 0.0004 0.0001 0.0001 0.0103 0.0004
Punctate Nuclear 0 0 0 0 0 0 0.0008 0.0001 0.0001 0.0003 0 0.0002 0.0214 0.0012 0 0 0.0004 0
Vacuole 0.0002 0.0001 0.0001 0 0 0.0001 0.0017 0.0003 0.0021 0.0003 0 0.0002 0.0013 0.0001 0 0.0001 0.0006 0.0002
Vacuole Periphery 0.0012 0.0007 0.0013 0.0004 0.0002 0.0013 0.0025 0.0006 0.0011 0.0009 0.0003 0.0005 0.0019 0.0007 0.0002 0.0003 0.001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 147.9578 142.7174 113.3712 136.6231 129.8855 120.3205 133.3418 116.4146 115.973 149.8794
Translational Efficiency 1.3922 1.3061 1.3091 1.0282 1.2094 1.5319 1.1107 1.0361 1.2065 1.1244

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of the Sec13p-Sec31p complex of the COPII vesicle coat; COPII coat is required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
Localization
Cell Percentages ER (74%)
Cell Cycle Regulation No
Subcompartmental Group ER-1

Sec31

Sec31


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec31-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available