Standard name
Human Ortholog
Description Protein related to mammalian high mobility group (HMG) proteins; nuclear protein; essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.68 0.51 0.44 0.52 0.28 0.35 0.32 – 0.4 0.32 0.35 0.34 0.69 0.55 0.55 0.08 0.07 0.05 0 0 0 0 0 0
Nucleus 0.08 0.13 0.05 0 0 0 0 – 0 0 0.06 0.05 0.06 0.06 0 0.18 0.14 0.19 0.08 0.09 0.05 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.94 0.95 0.91 0.94 0.91 0.88 0.91 – 0.91 0.89 0.88 0.87 0.97 0.93 0.88 0.82 0.9 0.86 0.89 0.87 0.9 0.89 0.86 0.87
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 2 1 0 1 1 0 0 0 0 0 0 0 4 0
Bud Neck 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 1 8 6
Bud Site 0 0 0 0 0 2 0 – 1 1 0 5 0 4 6 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1 5 8 4 7 10 – 8 18 11 13 3 11 38 11 9 9 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 3 5 5 0 0 0 0 0 0
Endosome 0 0 0 1 2 0 2 – 3 3 3 2 0 1 6 0 1 0 0 0 1 0 2 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 5 1
Mitochondria 97 76 65 129 92 91 135 – 160 157 135 129 162 304 305 31 23 19 0 1 0 3 6 8
Nucleus 12 19 8 4 14 7 16 – 15 20 22 19 15 32 20 70 47 65 11 12 6 9 15 11
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 2 3 2
Nucleolus 133 140 135 232 295 230 383 – 361 439 337 333 228 515 486 321 306 297 125 125 131 298 543 478
Peroxisomes 0 0 0 2 4 0 0 – 1 2 2 1 0 2 5 0 1 0 0 0 1 0 4 2
SpindlePole 0 1 1 5 9 10 13 – 10 11 12 12 0 7 18 2 0 1 2 0 1 6 21 14
Vac/Vac Membrane 0 1 0 2 5 2 7 – 2 4 3 7 0 1 1 7 4 3 2 3 0 6 7 14
Unique Cell Count 142 148 148 246 324 261 419 396 493 384 382 234 552 553 391 341 347 141 144 146 334 629 549
Labelled Cell Count 242 238 214 383 425 349 566 561 656 527 522 408 879 886 445 396 399 141 144 146 334 629 549


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 50.0 46.6 46.4 43.1 35.0 20.2 29.2 – 25.8 27.6 24.5 24.6 54.3 51.9 48.4 53.7 60.8 63.1 44.0 49.1 50.4
Std Deviation (1e-4) 18.8 16.8 26.1 22.4 13.7 8.6 15.0 – 13.3 14.2 12.5 13.0 17.3 21.0 23.8 28.4 28.7 30.8 16.1 18.5 19.2
Intensity Change (Log2) – – – -0.11 -0.41 -1.2 -0.67 – -0.85 -0.75 -0.92 -0.92 0.23 0.16 0.06 0.21 0.39 0.44 -0.08 0.08 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0.2 0 0 0 0 1.6 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.6 -3.3 -1.8 -2.6 – -0.7 -2.7 -1.9 -2.2 4.9 2.4 2.4 -9.7 -9.8 -10.4
Nucleus 0 -0.5 -1.4 -0.8 – -0.8 -0.7 0.1 -0.2 0.4 0.2 -1.0 3.7 2.7 3.8
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.2 -0.1 -1.0 0.1 – 0 -0.8 -1.1 -1.3 2.7 0.9 -1.1 -2.6 -0.5 -1.7
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 38.7786 45.2675 40.9245 37.2571 35.5886 37.6605 48.0537 59.8363 53.4794 48.2442 48.9385 51.5803 49.9978 54.9419 51.7756 43.7235 45.9184 48.7519
Actin 0.0011 0 0 0 0 0 0.0112 0 0.0052 0.001 0.0046 0.0014 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.0001 0 0 0.0012 0.0002 0 0 0 0 0 0 0
Bud Neck 0.0001 0 0 0 0 0 0.0012 0 0 0.0015 0.0002 0.0001 0 0 0 0 0 0
Bud Periphery 0 0 0 0 0 0 0.0003 0 0 0.0024 0.0004 0 0 0 0 0 0 0
Bud Site 0.0001 0 0 0 0 0 0.0004 0 0.0001 0.0214 0.0004 0 0.0001 0 0 0 0 0
Cell Periphery 0.0001 0 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0.0008 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001
Cytoplasm 0.0009 0 0 0 0 0 0.001 0 0 0.0001 0.0007 0.0001 0 0 0 0 0 0
Cytoplasmic Foci 0.0004 0 0 0 0.0285 0.0001 0.0144 0 0.0006 0.0023 0.0011 0.0017 0.0024 0 0.0024 0.0039 0.0006 0.0001
Eisosomes 0 0 0 0 0 0 0.0002 0 0 0.0001 0.0002 0 0.0002 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0009 0 0 0 0 0 0.0018 0 0 0 0.0021 0.0004 0 0 0 0 0 0
Endosome 0.0042 0 0 0 0 0 0.0123 0 0.0003 0.0004 0.0195 0.0055 0 0 0 0.0014 0 0
Golgi 0.0004 0 0 0 0 0 0.0033 0 0.0002 0.0003 0.0021 0.0015 0 0 0 0.0016 0 0
Lipid Particles 0.0003 0 0 0 0.001 0.0003 0.0134 0 0.0009 0.0007 0.0043 0.0022 0.0011 0 0.0003 0.0426 0.0029 0.0002
Mitochondria 0.0008 0 0.0012 0 0.0082 0.0001 0.013 0.0001 0.0045 0.0036 0.0721 0.0014 0.0094 0.0001 0.0014 0.0057 0.0605 0.001
None 0.0005 0 0 0 0.0002 0 0.0012 0 0 0.0002 0.0018 0.0002 0.0003 0 0 0.0001 0 0
Nuclear Periphery 0.0046 0 0 0 0 0 0.005 0 0 0 0.0294 0.0024 0.0001 0 0 0 0 0
Nucleolus 0.94 0.9159 0.9552 0.9438 0.9217 0.9517 0.8745 0.9275 0.9236 0.8631 0.8206 0.9323 0.9518 0.9409 0.9395 0.9095 0.9058 0.9501
Nucleus 0.0438 0.0839 0.0432 0.056 0.0393 0.0476 0.0366 0.0722 0.062 0.0984 0.0316 0.0491 0.0331 0.0587 0.0561 0.032 0.0268 0.0483
Peroxisomes 0.0001 0 0 0 0.0005 0 0.0058 0 0.002 0.0005 0.0029 0.0003 0.0004 0 0.0001 0.002 0.0009 0
Punctate Nuclear 0.0007 0.0001 0 0 0.0002 0.0001 0.0017 0 0.0001 0.0001 0.002 0.0005 0.0002 0 0.0001 0.0003 0.0001 0.0001
Vacuole 0.0004 0 0 0 0.0001 0 0.001 0.0001 0.0001 0.0014 0.001 0.0003 0.0002 0.0001 0.0001 0.0002 0.0003 0.0001
Vacuole Periphery 0.0004 0 0.0001 0 0.0002 0 0.0014 0 0.0001 0.0004 0.0026 0.0004 0.0005 0.0001 0.0001 0.0005 0.0016 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 237.7825 190.5737 177.1559 190.7906 195.9118 147.5074 190.5715 160.7786 140.2482 175.2402
Translational Efficiency 2.5015 3.5114 3.1538 2.5092 2.7995 3.3775 2.1243 2.7725 2.6124 2.5414

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1928 470 169 232 1857 2551 194 1572 3785 3021 363 1804

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2637.15 3553.79 4117.58 4726.40 2703.90 3283.30 4364.97 4260.53 2669.90 3325.38 4249.79 4320.44
Standard Deviation 762.84 1019.71 919.70 1474.20 787.90 954.18 1063.12 1480.86 775.96 969.64 1006.51 1488.20
Intensity Change Log 2 — 0.430379 0.642817 0.841762 — 0.280105 0.690930 0.655991 — 0.356259 0.667375 0.750705

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000295 0.000277 0.000324 0.000685 0.000306 0.000389 0.000275 0.000680 0.000300 0.000372 0.000298 0.000681
Bud Neck 0.000780 0.000960 0.002187 0.005397 0.000719 0.001410 0.001550 0.005190 0.000750 0.001340 0.001846 0.005217
Bud Site 0.000275 0.000417 0.000967 0.001545 0.000263 0.000436 0.000619 0.001359 0.000269 0.000433 0.000781 0.001383
Cell Periphery 0.000012 0.000023 0.000019 0.000056 0.000011 0.000024 0.000013 0.000122 0.000012 0.000023 0.000016 0.000113
Cytoplasm 0.000064 0.000535 0.000040 0.000155 0.000045 0.000181 0.000025 0.000597 0.000054 0.000236 0.000032 0.000540
Cytoplasmic Foci 0.008402 0.012326 0.022745 0.015427 0.008978 0.007912 0.016076 0.011654 0.008685 0.008598 0.019181 0.012139
Eisosomes 0.000444 0.000508 0.000295 0.000319 0.000690 0.000571 0.000300 0.000478 0.000565 0.000561 0.000298 0.000457
Endoplasmic Reticulum 0.000057 0.000068 0.000063 0.000079 0.000057 0.000072 0.000038 0.000104 0.000057 0.000071 0.000050 0.000101
Endosome 0.000006 0.000139 0.000016 0.000029 0.000008 0.000014 0.000007 0.000838 0.000007 0.000034 0.000012 0.000734
Golgi 0.000080 0.000110 0.000457 0.000313 0.000093 0.000092 0.000226 0.002025 0.000087 0.000095 0.000333 0.001805
Lipid Particles 0.000012 0.000158 0.000029 0.000034 0.000011 0.000025 0.000011 0.000110 0.000012 0.000046 0.000020 0.000100
Mitochondria 0.000910 0.001080 0.001440 0.004987 0.000991 0.001142 0.001517 0.003373 0.000949 0.001133 0.001481 0.003580
Mitotic Spindle 0.001374 0.000928 0.035689 0.007546 0.001058 0.001731 0.008938 0.002669 0.001219 0.001606 0.021392 0.003296
None 0.000358 0.000546 0.000280 0.000724 0.000368 0.000485 0.000300 0.000913 0.000363 0.000495 0.000291 0.000889
Nuclear Periphery 0.000049 0.000051 0.000404 0.000513 0.000036 0.000056 0.000091 0.000371 0.000043 0.000055 0.000237 0.000389
Nuclear Periphery Foci 0.000116 0.000043 0.000223 0.000136 0.000056 0.000065 0.000038 0.000177 0.000086 0.000062 0.000124 0.000171
Nucleolus 0.621831* 0.583173* 0.442754* 0.607672* 0.578838* 0.559731* 0.553318* 0.602116* 0.600738* 0.563378* 0.501843* 0.602831*
Nucleus 0.167729* 0.183718* 0.064353 0.185370* 0.175420* 0.280049* 0.080940 0.297508* 0.171503* 0.265062* 0.073218 0.283087*
Peroxisomes 0.196522* 0.213304* 0.426904* 0.165527* 0.231201* 0.144624* 0.334798* 0.064236 0.213536* 0.155309* 0.377679* 0.077262
Vacuole 0.000243 0.000879 0.000373 0.001238 0.000337 0.000328 0.000317 0.002197 0.000289 0.000414 0.000343 0.002073
Vacuole Periphery 0.000442 0.000757 0.000439 0.002249 0.000514 0.000662 0.000602 0.003284 0.000477 0.000677 0.000526 0.003151

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.87 -0.86 -4.73 -4.86 -3.95 -5.49 1.16 -14.49 -11.00 -12.07 -5.90 -0.01 -15.69 -12.17 -11.97
Bud Neck -1.35 -7.36 -6.97 -6.64 -4.85 -9.60 -7.02 -9.31 -8.02 -7.71 -9.33 -9.91 -10.40 -9.15 -8.03
Bud Site -1.25 -8.33 -5.77 -4.78 -2.70 -2.47 -5.85 -6.81 -5.25 -4.91 -2.66 -9.99 -7.73 -6.05 -4.61
Cell Periphery -1.91 -2.12 -4.71 -3.16 -3.71 -4.73 -0.82 -4.36 -3.89 -4.30 -5.07 -2.22 -4.60 -4.07 -4.40
Cytoplasm -0.98 0.81 -1.95 0.76 -2.78 -2.25 1.38 -2.13 -1.83 -2.17 -2.06 1.35 -2.14 -1.69 -2.19
Cytoplasmic Foci -1.41 -5.58 -3.42 -1.07 2.24 0.63 -3.11 -2.16 -2.50 1.51 -0.10 -6.07 -2.96 -2.61 3.24
Eisosomes -0.43 6.56 2.62 1.23 -0.53 3.04 10.54 4.69 2.48 -4.41 0.11 12.29 3.24 2.62 -4.70
Endoplasmic Reticulum -0.42 -0.82 -2.06 -0.63 -1.58 -2.79 5.90 -6.34 -3.78 -8.75 -2.06 1.03 -5.92 -3.64 -7.14
Endosome -1.12 -2.13 -5.65 0.91 -1.97 -1.29 0.19 -2.58 -2.56 -2.58 -1.42 -1.73 -2.59 -2.49 -2.57
Golgi -1.13 -12.56 -7.82 -5.16 3.37 0.04 -4.95 -2.38 -2.38 -2.21 -0.64 -12.15 -2.44 -2.42 -2.07
Lipid Particles -1.05 -1.67 -3.40 0.90 -0.24 -1.04 -0.03 -2.10 -1.75 -2.09 -1.41 -1.57 -2.16 -1.18 -1.94
Mitochondria -1.64 -3.01 -4.65 -4.44 -3.98 -2.06 -1.97 -7.57 -7.05 -4.69 -3.09 -3.26 -8.90 -8.20 -6.35
Mitotic Spindle 1.05 -3.68 -3.49 -3.78 2.97 -1.53 -2.12 -3.31 -1.80 1.61 -1.01 -4.19 -4.23 -3.16 3.70
None -1.98 2.11 -4.64 -1.57 -5.47 -2.70 2.06 -9.15 -6.28 -9.93 -3.37 2.93 -10.05 -6.45 -11.12
Nuclear Periphery -0.16 -1.22 -3.05 -3.03 -0.38 -2.96 -1.34 -2.85 -2.68 -2.28 -2.12 -1.42 -3.32 -3.19 -0.99
Nuclear Periphery Foci 1.10 -0.62 -0.20 -2.90 0.57 -0.53 1.19 -2.65 -2.41 -3.05 0.67 -0.45 -1.72 -2.68 -0.71
Nucleolus 3.77 10.82 1.31 -0.92 -7.05 3.00 2.02 -1.95 -4.20 -3.04 7.19 9.25 0.54 -4.22 -7.78
Nucleus -1.60 11.77 -1.42 -0.44 -7.00 -15.75 11.53 -14.33 -2.27 -21.38 -17.22 16.40 -15.12 -2.61 -24.56
Peroxisomes -2.01 -13.28 2.87 3.75 13.16 17.27* -7.41 34.98* 22.29 19.96* 15.26 -14.60 36.59* 21.22 26.78*
Vacuole -1.61 -3.55 -4.92 -0.98 -4.26 0.05 0.60 -4.72 -4.73 -4.78 -1.94 -2.30 -5.22 -4.76 -5.05
Vacuole Periphery -4.05 0.11 -5.38 -4.33 -5.37 -4.88 -1.15 -8.72 -8.23 -8.23 -7.68 -1.10 -9.56 -8.80 -9.28
Standard name
Human Ortholog
Description Protein related to mammalian high mobility group (HMG) proteins; nuclear protein; essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing
Localization
Cell Percentages nucleolus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Nhp2

Nhp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nhp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available