Standard name | |
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Human Ortholog | |
Description | Protein related to mammalian high mobility group (HMG) proteins; nuclear protein; essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 8 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 1 | 1 | 0 | 5 | 0 | 4 | 6 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 1 | 5 | 8 | 4 | 7 | 10 | – | 8 | 18 | 11 | 13 | 3 | 11 | 38 | 11 | 9 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 1 | 2 | 0 | 2 | – | 3 | 3 | 3 | 2 | 0 | 1 | 6 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 1 |
Mitochondria | 97 | 76 | 65 | 129 | 92 | 91 | 135 | – | 160 | 157 | 135 | 129 | 162 | 304 | 305 | 31 | 23 | 19 | 0 | 1 | 0 | 3 | 6 | 8 |
Nucleus | 12 | 19 | 8 | 4 | 14 | 7 | 16 | – | 15 | 20 | 22 | 19 | 15 | 32 | 20 | 70 | 47 | 65 | 11 | 12 | 6 | 9 | 15 | 11 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 2 |
Nucleolus | 133 | 140 | 135 | 232 | 295 | 230 | 383 | – | 361 | 439 | 337 | 333 | 228 | 515 | 486 | 321 | 306 | 297 | 125 | 125 | 131 | 298 | 543 | 478 |
Peroxisomes | 0 | 0 | 0 | 2 | 4 | 0 | 0 | – | 1 | 2 | 2 | 1 | 0 | 2 | 5 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 4 | 2 |
SpindlePole | 0 | 1 | 1 | 5 | 9 | 10 | 13 | – | 10 | 11 | 12 | 12 | 0 | 7 | 18 | 2 | 0 | 1 | 2 | 0 | 1 | 6 | 21 | 14 |
Vac/Vac Membrane | 0 | 1 | 0 | 2 | 5 | 2 | 7 | – | 2 | 4 | 3 | 7 | 0 | 1 | 1 | 7 | 4 | 3 | 2 | 3 | 0 | 6 | 7 | 14 |
Unique Cell Count | 142 | 148 | 148 | 246 | 324 | 261 | 419 | 396 | 493 | 384 | 382 | 234 | 552 | 553 | 391 | 341 | 347 | 141 | 144 | 146 | 334 | 629 | 549 | |
Labelled Cell Count | 242 | 238 | 214 | 383 | 425 | 349 | 566 | 561 | 656 | 527 | 522 | 408 | 879 | 886 | 445 | 396 | 399 | 141 | 144 | 146 | 334 | 629 | 549 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 50.0 | 46.6 | 46.4 | 43.1 | 35.0 | 20.2 | 29.2 | – | 25.8 | 27.6 | 24.5 | 24.6 | 54.3 | 51.9 | 48.4 | 53.7 | 60.8 | 63.1 | 44.0 | 49.1 | 50.4 |
Std Deviation (1e-4) | 18.8 | 16.8 | 26.1 | 22.4 | 13.7 | 8.6 | 15.0 | – | 13.3 | 14.2 | 12.5 | 13.0 | 17.3 | 21.0 | 23.8 | 28.4 | 28.7 | 30.8 | 16.1 | 18.5 | 19.2 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.41 | -1.2 | -0.67 | – | -0.85 | -0.75 | -0.92 | -0.92 | 0.23 | 0.16 | 0.06 | 0.21 | 0.39 | 0.44 | -0.08 | 0.08 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | – | 0 | 0.2 | 0 | 0 | 0 | 0 | 1.6 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.6 | -3.3 | -1.8 | -2.6 | – | -0.7 | -2.7 | -1.9 | -2.2 | 4.9 | 2.4 | 2.4 | -9.7 | -9.8 | -10.4 |
Nucleus | 0 | -0.5 | -1.4 | -0.8 | – | -0.8 | -0.7 | 0.1 | -0.2 | 0.4 | 0.2 | -1.0 | 3.7 | 2.7 | 3.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.2 | -0.1 | -1.0 | 0.1 | – | 0 | -0.8 | -1.1 | -1.3 | 2.7 | 0.9 | -1.1 | -2.6 | -0.5 | -1.7 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 38.7786 | 45.2675 | 40.9245 | 37.2571 | 35.5886 | 37.6605 | 48.0537 | 59.8363 | 53.4794 | 48.2442 | 48.9385 | 51.5803 | 49.9978 | 54.9419 | 51.7756 | 43.7235 | 45.9184 | 48.7519 |
Actin | 0.0011 | 0 | 0 | 0 | 0 | 0 | 0.0112 | 0 | 0.0052 | 0.001 | 0.0046 | 0.0014 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0012 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0012 | 0 | 0 | 0.0015 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0.0024 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0.0214 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 |
Cytoplasm | 0.0009 | 0 | 0 | 0 | 0 | 0 | 0.001 | 0 | 0 | 0.0001 | 0.0007 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0004 | 0 | 0 | 0 | 0.0285 | 0.0001 | 0.0144 | 0 | 0.0006 | 0.0023 | 0.0011 | 0.0017 | 0.0024 | 0 | 0.0024 | 0.0039 | 0.0006 | 0.0001 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0009 | 0 | 0 | 0 | 0 | 0 | 0.0018 | 0 | 0 | 0 | 0.0021 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0042 | 0 | 0 | 0 | 0 | 0 | 0.0123 | 0 | 0.0003 | 0.0004 | 0.0195 | 0.0055 | 0 | 0 | 0 | 0.0014 | 0 | 0 |
Golgi | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0033 | 0 | 0.0002 | 0.0003 | 0.0021 | 0.0015 | 0 | 0 | 0 | 0.0016 | 0 | 0 |
Lipid Particles | 0.0003 | 0 | 0 | 0 | 0.001 | 0.0003 | 0.0134 | 0 | 0.0009 | 0.0007 | 0.0043 | 0.0022 | 0.0011 | 0 | 0.0003 | 0.0426 | 0.0029 | 0.0002 |
Mitochondria | 0.0008 | 0 | 0.0012 | 0 | 0.0082 | 0.0001 | 0.013 | 0.0001 | 0.0045 | 0.0036 | 0.0721 | 0.0014 | 0.0094 | 0.0001 | 0.0014 | 0.0057 | 0.0605 | 0.001 |
None | 0.0005 | 0 | 0 | 0 | 0.0002 | 0 | 0.0012 | 0 | 0 | 0.0002 | 0.0018 | 0.0002 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 |
Nuclear Periphery | 0.0046 | 0 | 0 | 0 | 0 | 0 | 0.005 | 0 | 0 | 0 | 0.0294 | 0.0024 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.94 | 0.9159 | 0.9552 | 0.9438 | 0.9217 | 0.9517 | 0.8745 | 0.9275 | 0.9236 | 0.8631 | 0.8206 | 0.9323 | 0.9518 | 0.9409 | 0.9395 | 0.9095 | 0.9058 | 0.9501 |
Nucleus | 0.0438 | 0.0839 | 0.0432 | 0.056 | 0.0393 | 0.0476 | 0.0366 | 0.0722 | 0.062 | 0.0984 | 0.0316 | 0.0491 | 0.0331 | 0.0587 | 0.0561 | 0.032 | 0.0268 | 0.0483 |
Peroxisomes | 0.0001 | 0 | 0 | 0 | 0.0005 | 0 | 0.0058 | 0 | 0.002 | 0.0005 | 0.0029 | 0.0003 | 0.0004 | 0 | 0.0001 | 0.002 | 0.0009 | 0 |
Punctate Nuclear | 0.0007 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0.0017 | 0 | 0.0001 | 0.0001 | 0.002 | 0.0005 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
Vacuole | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 | 0.001 | 0.0001 | 0.0001 | 0.0014 | 0.001 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 |
Vacuole Periphery | 0.0004 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0014 | 0 | 0.0001 | 0.0004 | 0.0026 | 0.0004 | 0.0005 | 0.0001 | 0.0001 | 0.0005 | 0.0016 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 237.7825 | 190.5737 | 177.1559 | 190.7906 | 195.9118 | 147.5074 | 190.5715 | 160.7786 | 140.2482 | 175.2402 |
Translational Efficiency | 2.5015 | 3.5114 | 3.1538 | 2.5092 | 2.7995 | 3.3775 | 2.1243 | 2.7725 | 2.6124 | 2.5414 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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