Standard name
Human Ortholog
Description rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0.12 0.18 0.12 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0.16 0 0.1 0 0.15 0.05 – 0.14 0.11 0.17 0.1 0.26 0.13 0.11 0.08 0.1 0.09 0 0 0 0 0 0
Nucleus 0.13 0.14 0 0.17 0.19 0.17 0.15 – 0.18 0.17 0.17 0.15 0.11 0.08 0.09 0.15 0.14 0.15 0.05 0.11 0 0.06 0.05 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.97 0.93 1.0 0.88 0.78 0.81 0.83 – 0.77 0.8 0.79 0.8 0.94 0.88 0.78 0.69 0.76 0.8 0.89 0.84 0.91 0.87 0.8 0.75
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.07 – 0 0.07 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0.05 0.09
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 0 0 0 1 3 – 2 2 1 1 0 0 1 0 0 0 1 0 0 0 4 4
Bud Neck 0 0 0 0 0 2 5 – 4 4 3 3 1 1 1 0 0 0 0 0 0 0 2 2
Bud Site 0 1 0 0 0 5 1 – 8 9 11 13 0 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2 0 7 10 9 9 – 16 13 9 10 3 26 50 26 11 8 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 4 2 0 0 0 0 0 0 0
Endosome 0 1 0 1 6 3 27 – 5 13 17 5 1 0 4 2 0 0 0 0 0 0 2 6
Golgi 0 0 0 0 0 0 1 – 0 0 0 1 0 0 0 0 0 1 0 0 0 0 1 1
Mitochondria 22 18 0 21 13 46 26 – 55 53 74 42 53 53 47 11 9 9 0 0 0 2 2 7
Nucleus 19 16 1 34 57 53 79 – 74 82 77 62 23 31 37 22 13 15 7 11 2 15 14 4
Nuclear Periphery 0 0 0 1 0 1 1 – 9 8 3 4 0 0 1 0 0 0 0 0 0 0 0 1
Nucleolus 138 108 23 181 232 257 433 – 315 394 352 334 192 345 330 98 69 78 128 94 57 217 228 213
Peroxisomes 0 0 0 1 0 0 2 – 2 1 0 0 2 3 8 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 5 7 10 34 – 15 35 34 34 2 8 11 0 0 0 4 3 1 6 15 27
Vac/Vac Membrane 0 1 0 0 8 3 4 – 5 2 2 4 1 1 2 2 1 1 1 1 1 1 11 10
Unique Cell Count 143 116 23 205 297 317 522 407 492 445 417 205 394 423 142 91 97 145 113 63 251 286 286
Labelled Cell Count 179 148 24 251 333 390 625 510 617 583 513 278 469 493 165 105 112 145 113 63 251 286 286


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.0 22.1 15.1 18.0 15.6 9.8 12.1 – 10.0 10.1 9.3 9.6 23.7 21.6 20.8 28.3 28.0 34.7 21.8 23.6 22.6
Std Deviation (1e-4) 5.9 6.6 3.7 7.0 5.2 2.8 3.9 – 3.5 4.1 2.8 2.8 8.9 10.1 10.1 17.1 15.3 16.7 7.3 8.6 9.2
Intensity Change (Log2) – – – 0.25 0.05 -0.63 -0.32 – -0.59 -0.58 -0.7 -0.66 0.65 0.51 0.46 0.91 0.89 1.2 0.53 0.65 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 2.8 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.7 -2.5 -2.3 -2.2 – -2.6 -2.4 -2.4 -2.4 -1.2 -1.8 -2.5 -3.1 -2.6 -2.3
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.4091 14.179 13.2729 13.3591 13.0975 13.9148 18.1236 23.0818 20.9678 19.5752 17.8457 19.8031 21.3271 21.7793 20.9551 17.1073 19.3752 18.9799
Actin 0.0215 0 0.0068 0 0.0002 0.0005 0.0239 0 0.0021 0.0142 0.0001 0.0012 0.0002 0.0002 0 0 0 0
Bud 0.0002 0 0.0002 0 0 0.0002 0.0004 0 0.0001 0.0003 0 0 0 0 0 0.0002 0 0
Bud Neck 0.0002 0 0.0001 0 0.0001 0.0001 0.0004 0 0.0001 0.0019 0.0003 0.0001 0.0002 0.0001 0 0.0001 0.0002 0.0001
Bud Periphery 0.0002 0 0.0004 0 0.0001 0.0003 0.0004 0 0.0001 0.0005 0.0001 0 0 0 0 0.0002 0.0001 0
Bud Site 0.0007 0 0.0006 0 0.0001 0.0001 0.0019 0 0.0001 0.0028 0.0001 0 0.0002 0.0001 0 0.0002 0.0001 0
Cell Periphery 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0.0001 0.0001 0.0003 0.0003 0.0001 0.0001 0 0 0.0003 0.0011 0
Cytoplasm 0.0028 0 0.0001 0 0.0001 0.0001 0.0117 0 0.0001 0.0002 0.0001 0 0.0003 0.0317 0 0 0 0
Cytoplasmic Foci 0.0377 0.0009 0.0061 0.0002 0.0439 0.0005 0.0145 0 0.0014 0.0017 0.0089 0.0082 0.0304 0.0002 0.0001 0.0002 0.0004 0.0003
Eisosomes 0.0003 0 0.0001 0 0 0 0.0002 0 0 0.0005 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0018 0 0 0 0 0.0003 0.0036 0 0.0001 0.0006 0.0001 0 0 0 0 0 0 0
Endosome 0.0183 0.0035 0.0005 0 0.0006 0.0049 0.0315 0 0.0028 0.0039 0.0058 0.0024 0.0015 0 0 0 0.0001 0
Golgi 0.0044 0.0014 0.0007 0 0.0008 0.0015 0.0143 0 0.0038 0.016 0.0016 0.0048 0.0006 0 0 0 0 0
Lipid Particles 0.0144 0.0072 0.0014 0.0005 0.0082 0.0031 0.0218 0 0.0011 0.0009 0.0508 0.0029 0.0076 0 0.0001 0.0003 0.002 0.0002
Mitochondria 0.0157 0.0022 0.0098 0.0008 0.0032 0.0086 0.0072 0.0001 0.002 0.0069 0.0029 0.0013 0.0014 0.0001 0.0001 0.001 0.0077 0.0002
None 0.0036 0 0.0004 0 0 0 0.0044 0 0.0001 0.0006 0.0001 0 0.0008 0 0 0 0 0
Nuclear Periphery 0.0035 0.0001 0 0 0.0001 0.0004 0.0114 0 0.0001 0.0008 0.0002 0 0.0003 0 0 0 0 0
Nucleolus 0.7819 0.8294 0.8929 0.9223 0.8789 0.9015 0.7586 0.8928 0.8992 0.8787 0.8624 0.9014 0.8635 0.8463 0.9198 0.8705 0.924 0.923
Nucleus 0.0636 0.1512 0.0731 0.0676 0.0493 0.0754 0.0773 0.1062 0.0848 0.0677 0.0616 0.0759 0.0766 0.1211 0.0797 0.1255 0.0595 0.0759
Peroxisomes 0.0215 0.0019 0.0061 0 0.013 0.0007 0.0041 0 0.0008 0.0007 0.0015 0.0012 0.0042 0 0 0 0.0005 0
Punctate Nuclear 0.0049 0.0002 0.0004 0.008 0.0008 0.0004 0.0044 0.0003 0.0005 0.0004 0.001 0.0003 0.0114 0.0001 0.0001 0.0003 0.0004 0.0002
Vacuole 0.0013 0.0009 0.0002 0.0002 0.0002 0.0007 0.0051 0.0002 0.0003 0.0005 0.0006 0.0001 0.0002 0.0001 0 0.0007 0.0025 0
Vacuole Periphery 0.0011 0.0008 0.0001 0.0002 0.0002 0.0006 0.0025 0.0002 0.0004 0.0003 0.0013 0.0001 0.0003 0 0 0.0003 0.0013 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 54.5192 59.6481 65.8524 65.7219 64.3564 43.9166 51.4843 62.5471 54.9334 55.115
Translational Efficiency 3.5202 3.189 2.5256 2.2592 2.6459 3.1332 2.3334 1.7184 1.9773 2.4484

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1962 1156 433 1900 121 1884 329 1885 2083 3040 762 3785

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1463.72 1653.87 1992.88 2197.23 1613.71 1723.55 2206.17 1959.28 1472.43 1697.05 2084.97 2078.73
Standard Deviation 357.46 492.13 439.98 584.99 352.50 387.82 463.76 465.21 358.90 431.81 462.63 541.96
Intensity Change Log 2 — 0.176206 0.445215 0.586046 — 0.095002 0.451163 0.279942 — 0.134196 0.448337 0.433647

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000243 0.000320 0.000270 0.000514 0.000193 0.000288 0.000218 0.000334 0.000240 0.000300 0.000247 0.000424
Bud Neck 0.000805 0.001275 0.001782 0.003416 0.001635 0.001737 0.001170 0.001660 0.000853 0.001561 0.001518 0.002541
Bud Site 0.000181 0.000758 0.000579 0.000701 0.000280 0.000504 0.000333 0.000628 0.000187 0.000601 0.000473 0.000665
Cell Periphery 0.000017 0.000081 0.000013 0.000049 0.000008 0.000033 0.000007 0.000020 0.000017 0.000052 0.000010 0.000034
Cytoplasm 0.000101 0.000894 0.000039 0.000711 0.000030 0.000369 0.000013 0.000061 0.000097 0.000569 0.000028 0.000388
Cytoplasmic Foci 0.001945 0.006274 0.004764 0.003772 0.010472 0.003076 0.003774 0.001881 0.002440 0.004292 0.004336 0.002830
Eisosomes 0.000474 0.000334 0.000217 0.000314 0.000223 0.000373 0.000227 0.000338 0.000460 0.000358 0.000221 0.000326
Endoplasmic Reticulum 0.000131 0.000167 0.000085 0.000114 0.000293 0.000426 0.000054 0.000086 0.000141 0.000327 0.000071 0.000100
Endosome 0.000066 0.000210 0.000013 0.000072 0.000025 0.000148 0.000007 0.000026 0.000063 0.000172 0.000011 0.000049
Golgi 0.000091 0.000222 0.000282 0.000188 0.000163 0.000186 0.000156 0.000112 0.000095 0.000200 0.000228 0.000150
Lipid Particles 0.000018 0.000241 0.000012 0.000026 0.000042 0.000436 0.000006 0.000010 0.000020 0.000362 0.000009 0.000018
Mitochondria 0.000789 0.001446 0.001460 0.003067 0.000522 0.001028 0.000776 0.001114 0.000774 0.001187 0.001164 0.002094
Mitotic Spindle 0.000580 0.001166 0.010708 0.004534 0.001259 0.000831 0.002420 0.002027 0.000619 0.000958 0.007130 0.003286
None 0.000525 0.001121 0.000292 0.000583 0.000269 0.000454 0.000267 0.000307 0.000511 0.000707 0.000281 0.000446
Nuclear Periphery 0.000050 0.000048 0.000086 0.000266 0.000046 0.000104 0.000033 0.000073 0.000050 0.000083 0.000063 0.000170
Nuclear Periphery Foci 0.000178 0.000190 0.000063 0.000143 0.000863 0.000187 0.000031 0.000062 0.000218 0.000188 0.000049 0.000103
Nucleolus 0.650525* 0.621496* 0.633815* 0.642518* 0.665734* 0.614528* 0.664511* 0.676730* 0.651409* 0.617178* 0.647069* 0.659556*
Nucleus 0.255748* 0.261184* 0.138911* 0.279512* 0.177826* 0.301048* 0.159113* 0.232129* 0.251222* 0.285889* 0.147634* 0.255914*
Peroxisomes 0.086669 0.100354* 0.205930* 0.057648 0.139432* 0.072908 0.166169* 0.081287 0.089734 0.083345 0.188763* 0.069421
Vacuole 0.000410 0.001340 0.000269 0.000614 0.000379 0.000660 0.000258 0.000404 0.000408 0.000919 0.000264 0.000509
Vacuole Periphery 0.000452 0.000880 0.000410 0.001237 0.000307 0.000674 0.000457 0.000712 0.000444 0.000752 0.000430 0.000976

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.94 -1.63 -10.65 -6.20 -7.95 -3.71 -0.83 -4.06 -1.49 -4.01 -4.94 -0.77 -9.75 -5.98 -8.26
Bud Neck -4.63 -5.30 -10.45 -8.24 -5.64 0.07 0.95 0.19 0.36 -3.23 -5.43 -5.45 -10.93 -5.38 -6.03
Bud Site -3.28 -6.90 -8.34 0.24 -1.60 -2.36 -0.96 -1.20 -0.14 -1.03 -4.64 -7.86 -3.46 -0.13 -1.34
Cell Periphery -1.49 0.68 -4.04 0.66 -4.95 -3.75 0.78 -3.18 1.99 -3.72 -2.06 1.25 -3.33 0.93 -5.84
Cytoplasm -1.54 1.36 -1.55 0.54 -1.70 -1.91 2.04 -1.98 1.79 -3.17 -2.20 1.66 -1.47 1.04 -1.81
Cytoplasmic Foci -5.09 -4.45 -3.29 2.19 0.96 1.09 1.00 1.27 1.50 2.94 -2.91 -3.36 -1.13 2.09 2.51
Eisosomes 4.70 9.37 5.34 0.89 -4.37 -4.77 -0.19 -3.82 1.34 -5.38 3.76 9.63 5.12 1.79 -6.86
Endoplasmic Reticulum -1.31 4.15 0.81 1.69 -2.23 -0.44 1.93 1.68 1.28 -7.37 -1.13 5.67 2.88 1.36 -3.99
Endosome -1.25 1.12 -0.16 1.25 -2.62 -2.21 2.21 -0.50 1.95 -1.48 -1.67 1.20 0.17 2.16 -2.92
Golgi -2.73 -10.70 -2.55 0.52 2.20 -0.46 0.17 1.88 1.26 3.22 -2.62 -10.25 -2.75 1.08 3.35
Lipid Particles -1.25 0.90 -0.84 1.19 -1.51 -1.36 1.43 1.26 1.47 -1.94 -1.79 1.54 -0.03 1.79 -1.77
Mitochondria -2.39 -1.79 -3.27 -2.21 -2.09 -3.25 -3.14 -4.95 -0.48 -3.00 -2.67 -1.74 -3.67 -2.43 -2.32
Mitotic Spindle -0.88 -5.49 -4.20 -2.93 2.93 0.73 -1.14 -1.40 -3.17 0.33 -1.19 -5.76 -5.28 -4.10 3.04
None -2.95 2.08 -0.68 2.85 -5.17 -3.30 0.20 -0.87 2.54 -1.11 -1.73 2.22 0.49 3.26 -4.86
Nuclear Periphery 0.05 -1.62 -2.88 -2.94 -2.41 -1.18 1.09 -1.87 0.64 -5.23 -1.01 -0.76 -3.02 -1.88 -2.81
Nuclear Periphery Foci -0.29 1.98 0.40 0.69 -1.59 1.13 1.46 1.39 1.55 -2.13 0.11 2.69 1.59 1.50 -2.13
Nucleolus 3.68 2.03 1.54 -2.07 -0.79 3.07 0.03 -0.71 -8.57 -1.16 5.59 0.80 -1.02 -7.23 -1.68
Nucleus -0.62 12.98 -3.32 -2.30 -14.91 -6.96 1.08 -3.07 9.02 -6.83 -5.27 13.58 -0.92 4.88 -15.42
Peroxisomes -3.58 -15.23 10.47 11.78 19.06* 5.65 -2.08 4.91 -3.01 11.58 2.33 -17.39 8.00 6.22 21.76*
Vacuole -2.21 2.03 -2.84 1.61 -5.49 -1.77 1.82 -0.49 1.45 -2.32 -2.74 2.33 -1.92 2.02 -5.36
Vacuole Periphery -3.57 0.93 -6.66 -2.13 -7.20 -3.68 -3.53 -8.90 -0.31 -5.89 -3.88 0.35 -7.79 -2.27 -8.85
Standard name
Human Ortholog
Description rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes
Localization
Cell Percentages nucleolus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Nop6

Nop6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nop6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available