Standard name
Human Ortholog
Description Catalytic subunit of the COP9 signalosome (CSN) complex; acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling; involved in modulation of genes controlling amino acid and lipid metabolism, and ergosterol biosynthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.98 0.97 0.94 0.87 0.8 0.77 0.73 0.72 0.64 0.62 1.0 0.99 0.99 0.98 0.96 0.96 0.84 0.95 0.62 0.89 0.85 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0.09 0.33 0.41 0.36 0.41 0.48 0.56 0.55 0 0 0 0 0 0 0 0 0.2 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 7 3 15 2 3 6
Bud 0 1 0 2 5 6 16 10 10 16 17 21 0 0 0 0 0 0 5 1 9 7 13 13
Bud Neck 2 0 9 2 4 4 6 1 2 2 0 0 1 3 3 0 0 3 5 0 3 1 2 3
Bud Site 0 0 0 0 0 1 1 1 1 1 3 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 10 2 2 1 5 0 4 3 2 2 5 2 1 8 9 11 5 1 13 3 1 3
Cytoplasm 625 524 641 344 517 418 538 417 362 485 383 458 459 293 420 400 410 431 504 503 387 488 451 600
Endoplasmic Reticulum 3 1 2 1 0 0 0 0 0 1 0 2 1 2 0 18 30 27 2 0 7 1 0 2
Endosome 0 0 1 0 1 2 1 6 4 1 3 1 0 2 3 4 5 8 8 2 11 4 4 5
Golgi 0 1 3 1 0 1 0 0 0 0 0 0 0 0 1 0 2 1 1 2 5 1 1 2
Mitochondria 39 3 1 15 49 160 275 192 204 323 337 405 0 0 0 2 10 8 21 4 124 11 17 16
Nucleus 0 0 0 0 4 2 3 2 1 4 4 3 1 2 0 2 2 0 0 0 0 3 3 1
Nuclear Periphery 1 1 1 1 1 1 1 0 1 0 1 4 0 0 0 0 0 0 0 0 2 0 0 1
Nucleolus 0 0 0 0 0 0 1 2 2 1 3 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 1 2 1 0 1 0 0 0 1 0 0 3 0 1 0 0 1
SpindlePole 0 0 3 2 9 5 3 8 18 10 11 9 0 3 1 0 3 1 7 1 16 5 10 17
Vac/Vac Membrane 0 0 3 0 0 2 2 4 9 10 21 16 0 0 0 2 6 9 4 1 4 5 6 6
Unique Cell Count 648 528 655 353 550 483 670 540 498 671 603 735 460 297 425 407 428 449 600 530 629 551 529 695
Labelled Cell Count 672 532 674 370 593 603 852 644 620 858 785 924 467 307 429 437 478 499 600 530 629 551 529 695


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 4.1 4.0 3.9 4.2 3.5 3.4 3.5 3.3 3.1 2.9 3.0 5.2 5.1 5.0 5.8 5.6 5.5 5.1 5.4 5.4
Std Deviation (1e-4) 0.4 0.7 1.1 1.9 2.5 1.9 1.9 1.6 1.2 1.1 1.2 0.8 1.1 0.9 0.9 1.2 1.4 1.8 1.1 1.6 1.2
Intensity Change (Log2) – – – -0.03 0.05 -0.2 -0.24 -0.21 -0.31 -0.38 -0.45 -0.41 0.36 0.34 0.31 0.52 0.47 0.44 0.34 0.42 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.215 1.1153 0.8251 0.5423 0.7581 0.8543 -0.6843 0.0604 -0.2729 -0.2571 0.0797 0.0032 -1.1904 -0.5918 -0.9099 -0.3451 -0.6334 -0.6641
Actin 0.0005 0.0015 0.0037 0.0002 0.0015 0.0025 0.016 0.0001 0.0003 0.0002 0.0098 0.0003 0.0106 0.0003 0.0007 0.0001 0.0001 0.0051
Bud 0.0003 0.0029 0.0008 0 0.0007 0.0001 0.0002 0.0005 0.0002 0.0002 0.0019 0.0025 0.0002 0.0007 0.0055 0 0 0.0016
Bud Neck 0.0002 0.0001 0.0005 0.0001 0.0005 0.0006 0.0004 0.0001 0.0001 0 0.0002 0.0068 0.0018 0.0004 0.0016 0 0 0.0018
Bud Periphery 0.0002 0.0024 0.0024 0 0.0016 0.0001 0.0001 0.0005 0.0003 0.0003 0.0009 0.0021 0.0003 0.0005 0.0015 0 0 0.0013
Bud Site 0.0002 0.0045 0.0011 0 0.0004 0 0.0002 0.0052 0.0006 0 0.0015 0.0016 0.0041 0.0181 0.0105 0 0 0.0006
Cell Periphery 0.0001 0.0002 0.0001 0 0.0002 0 0.0001 0.0002 0 0 0.0001 0.0001 0.0001 0.0004 0.0002 0 0 0.0001
Cytoplasm 0.0313 0.0579 0.0361 0.0506 0.1079 0.0607 0.0276 0.0654 0.0617 0.059 0.028 0.0958 0.0634 0.1188 0.0896 0.152 0.0574 0.1486
Cytoplasmic Foci 0.0021 0.0043 0.0073 0.0033 0.0114 0.0091 0.0049 0.0024 0.0058 0.0029 0.052 0.0036 0.0016 0.0057 0.0038 0.0028 0.0015 0.0032
Eisosomes 0.0005 0.0002 0.0002 0.0001 0.0001 0.0001 0.0004 0.0001 0.0001 0 0.0004 0 0.0004 0.0001 0.0002 0 0 0.0001
Endoplasmic Reticulum 0.0011 0.0011 0.0006 0.0002 0.0022 0.0004 0.0006 0.0004 0.0006 0.0003 0.0012 0.0005 0.0006 0.0007 0.0007 0.0007 0.0002 0.0006
Endosome 0.0016 0.0006 0.0009 0.0001 0.0116 0.001 0.0014 0.0002 0.0007 0.0002 0.0054 0.0006 0.0005 0.0004 0.0003 0.0012 0.0001 0.0014
Golgi 0.0001 0.0002 0.0002 0 0.0015 0.0001 0.0008 0 0.0001 0 0.005 0 0.0005 0.0001 0.0001 0.0001 0 0.0005
Lipid Particles 0.0005 0.0013 0.0005 0 0.0005 0.0002 0.001 0.0001 0.0002 0.0001 0.0054 0.0001 0.0004 0.0001 0.0002 0.0001 0.0001 0.0016
Mitochondria 0.0003 0.0004 0.0004 0.0001 0.0199 0.0002 0.0006 0.0001 0.0003 0.0003 0.0111 0.0002 0.0004 0.0002 0.0003 0.0002 0 0.0004
None 0.9519 0.9164 0.9393 0.9442 0.8347 0.9209 0.9398 0.9217 0.9243 0.9335 0.842 0.8781 0.9134 0.8511 0.8761 0.8413 0.9401 0.8272
Nuclear Periphery 0.001 0.0006 0.0006 0.0001 0.0006 0.0001 0.0006 0.0002 0.0003 0.0005 0.0095 0.0004 0.0002 0.0002 0.0006 0.0002 0.0001 0.001
Nucleolus 0.0011 0.0004 0.0003 0 0.0001 0.0001 0.0009 0.0004 0.0002 0.0002 0.0014 0.0004 0.0002 0.0002 0.0004 0 0 0.0002
Nucleus 0.0021 0.0019 0.0005 0.0003 0.0008 0.0003 0.0017 0.001 0.0009 0.0007 0.0025 0.0048 0.0002 0.0006 0.0043 0.0004 0.0001 0.0019
Peroxisomes 0.0002 0.0003 0.0014 0.0002 0.0008 0.0007 0.0003 0 0.0003 0.0001 0.0012 0.0001 0.0005 0.0001 0.0002 0 0 0.0007
Punctate Nuclear 0.004 0.0024 0.0028 0.0002 0.0005 0.0027 0.0018 0.001 0.0024 0.0011 0.0185 0.0017 0.0002 0.0005 0.0026 0.0002 0.0001 0.0017
Vacuole 0.0004 0.0004 0.0002 0.0001 0.0017 0.0001 0.0004 0.0004 0.0003 0.0002 0.0005 0.0003 0.0002 0.0006 0.0005 0.0005 0.0001 0.0004
Vacuole Periphery 0.0001 0.0001 0 0 0.0008 0 0.0001 0 0 0 0.0017 0.0001 0 0 0.0001 0.0001 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.3361 2.2367 2.7662 4.0836 2.7033 2.5007 4.8999 3.8159 4.6477 5.6756
Translational Efficiency 1.0678 1.1318 0.8078 0.5231 1.1387 1.6581 1.02 0.9698 0.7842 0.6585

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1963 558 2579 112 1650 1950 1576 1133 3613 2508 4155 1245

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 587.05 733.89 771.40 817.49 629.75 682.05 806.20 826.55 606.55 693.58 784.60 825.73
Standard Deviation 72.18 88.14 88.36 85.56 71.61 80.41 94.03 127.81 75.00 84.97 92.11 124.62
Intensity Change Log 2 — 0.322080 0.393996 0.477718 — 0.115098 0.356359 0.392323 — 0.218668 0.374639 0.434153

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000164 0.000432 0.000168 0.000549 0.000056 0.000855 0.000224 0.005833 0.000115 0.000761 0.000189 0.005358
Bud Neck 0.024082 0.028367 0.010079 0.002440 0.008934 0.036859 0.003065 0.011393 0.017164 0.034970 0.007419 0.010588
Bud Site 0.008972 0.013938 0.013035 0.014534 0.001289 0.021026 0.007745 0.089264 0.005463 0.019449 0.011028 0.082541
Cell Periphery 0.000223 0.000225 0.000117 0.000137 0.000129 0.000169 0.000123 0.000414 0.000180 0.000182 0.000119 0.000389
Cytoplasm 0.444014* 0.337070* 0.424804* 0.363339* 0.478092* 0.434450* 0.335419* 0.445494* 0.459577* 0.412784* 0.390900* 0.438103*
Cytoplasmic Foci 0.250541* 0.348332* 0.006851 0.035505 0.227299* 0.223032* 0.010285 0.032457 0.239927* 0.250910* 0.008153 0.032731
Eisosomes 0.000324 0.000236 0.000018 0.000080 0.000176 0.000151 0.000033 0.000061 0.000256 0.000170 0.000024 0.000063
Endoplasmic Reticulum 0.001470 0.000834 0.000785 0.002967 0.000691 0.000522 0.001714 0.001326 0.001114 0.000592 0.001137 0.001474
Endosome 0.008636 0.028455 0.001216 0.007738 0.006730 0.017762 0.001679 0.013250 0.007766 0.020141 0.001391 0.012755
Golgi 0.003784 0.009192 0.000370 0.003200 0.001654 0.005570 0.000387 0.010808 0.002811 0.006376 0.000377 0.010124
Lipid Particles 0.013734 0.011087 0.000462 0.003692 0.010127 0.006042 0.001117 0.001155 0.012086 0.007165 0.000710 0.001384
Mitochondria 0.007510 0.014628 0.001663 0.033809 0.003727 0.012852 0.000585 0.004221 0.005782 0.013247 0.001254 0.006883
Mitotic Spindle 0.001654 0.004009 0.005620 0.017832 0.000079 0.012590 0.003241 0.076057 0.000935 0.010681 0.004718 0.070819
None 0.006482 0.002071 0.004939 0.002211 0.002725 0.003419 0.005567 0.005535 0.004766 0.003119 0.005177 0.005236
Nuclear Periphery 0.000434 0.000486 0.001323 0.001663 0.000418 0.000484 0.001444 0.001343 0.000426 0.000484 0.001369 0.001372
Nuclear Periphery Foci 0.000259 0.000640 0.000161 0.001863 0.000206 0.000266 0.000602 0.001465 0.000235 0.000349 0.000328 0.001501
Nucleolus 0.001597 0.001959 0.000501 0.000763 0.001172 0.001914 0.000557 0.000505 0.001403 0.001924 0.000522 0.000529
Nucleus 0.181786* 0.109160 0.506805* 0.246357* 0.225367* 0.142439* 0.591417* 0.156516* 0.201689* 0.135035* 0.538898* 0.164598*
Peroxisomes 0.006206 0.024369 0.000410 0.000959 0.003723 0.015025 0.000439 0.004956 0.005072 0.017104 0.000421 0.004596
Vacuole 0.036914 0.062590 0.020172 0.259761* 0.026930 0.062802 0.034019 0.131880 0.032355 0.062755 0.025424 0.143384
Vacuole Periphery 0.001215 0.001918 0.000502 0.000602 0.000475 0.001768 0.000339 0.006065 0.000877 0.001802 0.000440 0.005574

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.77 -0.11 -2.71 0.50 -2.45 -2.43 -3.26 -2.74 -1.46 -2.50 -2.52 -2.15 -2.70 -1.57 -2.58
Bud Neck -1.27 8.17 12.64 8.73 5.00 -13.37 5.98 -1.23 12.71 -7.40 -9.56 9.07 6.58 14.19 -2.56
Bud Site -2.25 -1.57 -0.34 0.75 0.26 -10.69 -4.28 -10.24 -4.54 -8.44 -8.66 -3.82 -9.06 -4.76 -7.70
Cell Periphery -0.08 5.91 4.92 5.06 -0.01 -2.98 1.38 -1.21 -0.38 -1.48 -0.04 6.02 0.02 0.03 -1.57
Cytoplasm 8.92 6.60 6.90 2.51 4.83 5.20 21.44 11.75 7.31 -5.67 6.95 17.78 12.20 6.53 0.79
Cytoplasmic Foci -8.76 50.82 31.08 28.32 -5.53 0.59 43.26 38.50 38.63 -8.83 -1.88 66.73 56.38 47.39 -11.50
Eisosomes 3.04 12.91 8.57 6.27 -9.78 2.53 16.99 12.09 10.50 -9.28 5.90 17.11 13.08 12.80 -15.85
Endoplasmic Reticulum 1.32 0.67 -3.49 -5.01 -4.63 1.14 -11.47 -9.93 -13.09 0.20 1.99 -2.32 -5.58 -13.72 -6.63
Endosome -7.32 14.42 0.40 6.11 -3.20 -8.95 6.60 -0.14 8.95 -7.04 -11.56 14.39 1.04 11.17 -8.34
Golgi -2.95 10.09 2.89 4.04 -0.88 -4.82 4.18 -1.41 1.95 -2.88 -4.78 10.31 -0.11 2.97 -2.95
Lipid Particles 1.55 15.56 6.97 3.78 -3.08 4.80 11.41 10.73 12.10 -4.40 6.79 19.37 16.99 11.56 -8.79
Mitochondria -2.63 9.05 -0.38 0.50 -1.18 -5.72 3.78 2.06 7.94 -4.01 -5.70 8.73 3.48 7.53 -2.01
Mitotic Spindle -1.67 -4.28 -1.50 -0.79 -0.68 -7.12 -3.69 -11.21 -8.32 -10.37 -6.81 -5.91 -11.10 -8.63 -10.20
None 5.12 2.26 3.55 -0.56 2.40 -0.92 -3.23 -4.14 -2.70 -1.35 2.51 0.23 -0.68 -3.25 -1.11
Nuclear Periphery -0.66 -20.29 -4.35 -4.22 -2.25 -0.60 -20.83 -17.69 -13.70 -5.67 -0.67 -28.68 -18.31 -15.03 -7.61
Nuclear Periphery Foci -1.82 1.17 -3.53 -2.99 -3.57 -0.82 -8.95 -7.15 -6.87 -5.34 -1.61 -6.25 -7.73 -7.21 -7.08
Nucleolus -1.22 4.01 0.29 1.13 -1.56 -1.45 1.18 -1.70 -0.73 -3.52 -1.83 3.27 -1.64 -0.47 -3.98
Nucleus 9.79 -49.72 -1.83 -4.80 13.05 13.82 -45.63 1.29 -8.87 39.40 14.92 -65.92 -1.81 -10.56 42.32
Peroxisomes -6.73 12.30 7.95 8.68 -1.33 -9.38 8.63 3.08 10.65 -4.00 -10.79 14.91 6.61 13.34 -4.20
Vacuole -5.83 3.75 -11.89 -10.92 -12.14 -14.63 -10.10 -28.07 -23.02 -22.82 -13.99 -4.61 -29.58 -25.17 -28.28
Vacuole Periphery -3.31 5.31 5.84 7.86 0.57 -7.92 1.40 -2.47 -0.10 -2.78 -6.40 4.92 -1.62 0.21 -2.65
Standard name
Human Ortholog
Description Catalytic subunit of the COP9 signalosome (CSN) complex; acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling; involved in modulation of genes controlling amino acid and lipid metabolism, and ergosterol biosynthesis
Localization
Cell Percentages cytoplasm (19%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rri1

Rri1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rri1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available