Standard name
Human Ortholog
Description ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; required for prospore membrane formation; regulates phospholipase Spo14p; shares functional similarity with Glo3p; GCS1 has a paralog, SPS18, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.05 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.06 0.05 0 0 0.1 0 0 0 0.05 0 0.08 0 0 0 0 0 0
Cytoplasm 0.91 0.91 0.86 0.88 0.82 0.82 0.77 0.67 0.73 0.63 0.65 0.55 0.94 0.99 0.98 0.93 0.96 0.96 0.65 0.87 0.71 0.87 0.89 0.91
Endoplasmic Reticulum 0.05 0.08 0.11 0.08 0.06 0.13 0.12 0.15 0.09 0.07 0.06 0.07 0 0 0 0 0 0 0.14 0 0.08 0.06 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.08 0 0.16 0.14 0.25 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.1 0 0 0.1 0 0.15 0.07 0.19 0 0 0 0.05 0 0 0 0 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 4 0 2 5 2 9 12 8 1 1 13 0 1 0 0 0 0 9 3 1 0 1 0
Bud 4 3 1 0 0 0 3 1 5 2 1 0 0 0 0 0 0 2 13 1 2 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 5 1 2 5 2 4 17 8 3 3 15 2 4 2 14 3 23 0 0 0 0 0 0
Cytoplasm 197 243 105 162 178 187 196 201 122 76 97 83 107 148 164 273 243 293 152 238 127 91 160 184
Endoplasmic Reticulum 11 21 14 14 12 30 30 46 15 8 9 10 4 0 1 2 5 7 32 9 13 6 6 4
Endosome 0 0 1 2 0 0 0 1 0 1 1 2 0 0 2 0 0 1 3 3 5 0 0 1
Golgi 4 4 2 2 6 1 2 1 0 0 0 0 0 1 0 3 1 2 4 2 3 0 0 0
Mitochondria 3 2 0 0 2 3 20 12 26 17 37 20 0 0 0 2 1 1 5 1 4 2 0 1
Nucleus 0 0 0 2 0 5 1 6 2 2 2 0 0 0 2 1 0 0 0 1 0 0 0 2
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 3 0 0 0 0 0
Vac/Vac Membrane 0 6 2 6 22 7 11 31 2 18 11 29 3 0 0 16 7 2 1 5 12 1 5 4
Unique Cell Count 217 266 122 185 217 229 255 299 167 120 150 151 114 149 168 293 252 305 235 273 179 105 181 204
Labelled Cell Count 226 288 126 192 230 237 276 329 188 128 163 174 116 155 171 311 260 331 235 273 179 105 181 204


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.2 23.6 17.0 16.4 15.5 13.2 12.1 12.7 10.9 9.8 9.9 10.2 17.5 20.0 19.2 26.7 26.5 27.0 18.2 19.0 20.6
Std Deviation (1e-4) 5.6 4.0 3.2 2.6 2.7 2.4 2.4 2.2 2.2 1.7 1.9 2.0 4.0 3.3 3.7 6.9 6.1 5.8 3.2 3.8 4.0
Intensity Change (Log2) – – – -0.05 -0.13 -0.36 -0.49 -0.42 -0.64 -0.79 -0.77 -0.73 0.04 0.24 0.18 0.65 0.64 0.67 0.1 0.16 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 3.3 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 2.2 0 0 0 3.2 0 0 0 0 0 2.7
Cytoplasm 0.4 -1.0 -1.1 -2.1 -3.9 -2.7 -4.1 -4.0 -5.5 2.0 4.4 3.7 2.3 3.7 3.7
Endoplasmic Reticulum -1.2 -2.0 0.4 0.1 1.0 -0.7 -1.3 -1.6 -1.4 -2.3 -4.2 -4.1 0 -3.9 -4.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.2 0 4.6 4.3 5.9 4.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.9 0 0 3.0 0 3.8 2.2 4.5 0 0 0 1.7 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0293 0.0035 0.0134 0.0126 0.0021 0.0036 0.0042 0.0008 0.0029 0.0026 0.0013 0.0023 0.1099 0.002 0.0516 0.6818 0.0054 0.0143
Bud 0.0024 0.0008 0.0033 0.002 0.0005 0.0003 0.0003 0.0003 0.0008 0.001 0.0002 0.0003 0.0004 0.0003 0.0018 0.0158 0.0009 0.0009
Bud Neck 0.0011 0.0004 0.001 0.0013 0.0006 0.0024 0.0006 0.0005 0.0012 0.0009 0.0008 0.002 0.0008 0.0003 0.0016 0.0019 0.0027 0.0038
Bud Periphery 0.003 0.0002 0.0014 0.0022 0.0003 0.0001 0.0001 0.0001 0.0003 0.0007 0 0.0002 0.0004 0 0.0012 0.0614 0.0001 0.0011
Bud Site 0.0034 0.0055 0.0045 0.0255 0.0004 0.0002 0.0004 0.0018 0.0006 0.0003 0.0001 0.001 0.0017 0.0008 0.0068 0.0034 0.0002 0.0005
Cell Periphery 0.0014 0.0001 0.0002 0.0005 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0 0.0002 0.0005 0.0001 0.0005 0.0023 0 0.0001
Cytoplasm 0.6573 0.9256 0.8048 0.8507 0.7362 0.8197 0.8753 0.8878 0.8776 0.8437 0.8643 0.8561 0.5259 0.8803 0.7434 0.0008 0.7883 0.8326
Cytoplasmic Foci 0.033 0.021 0.0396 0.0171 0.04 0.0394 0.0229 0.0371 0.0318 0.037 0.0263 0.0303 0.0377 0.0383 0.0406 0.0018 0.0571 0.0333
Eisosomes 0.001 0 0 0.0003 0 0 0 0 0 0 0 0.0001 0.0007 0 0.0008 0.0057 0 0
Endoplasmic Reticulum 0.0574 0.0098 0.0266 0.0047 0.0155 0.0188 0.0349 0.0244 0.0231 0.0181 0.0347 0.0358 0.0811 0.0285 0.0137 0.0145 0.0095 0.0178
Endosome 0.0941 0.0203 0.0709 0.0511 0.1452 0.0809 0.0324 0.0313 0.0391 0.0598 0.0561 0.048 0.0632 0.0385 0.0741 0.0254 0.0977 0.0624
Golgi 0.0254 0.0026 0.016 0.0109 0.0304 0.015 0.0075 0.004 0.0076 0.0051 0.0051 0.011 0.0211 0.0054 0.0172 0.0698 0.0242 0.0174
Lipid Particles 0.0093 0.0003 0.0015 0.0007 0.0013 0.0006 0.0012 0.003 0.0006 0.0013 0.0003 0.0005 0.0067 0.0001 0.0029 0.0036 0.0002 0.0018
Mitochondria 0.0228 0.0007 0.0047 0.007 0.0112 0.0045 0.0059 0.0004 0.0013 0.0009 0.001 0.0044 0.0041 0.0005 0.0065 0.0901 0.0026 0.0038
None 0.0044 0.0008 0.0006 0.0008 0.0005 0.0005 0.0013 0.0004 0.0009 0.0004 0.0006 0.0007 0.0377 0.0003 0.0009 0.0018 0.0006 0.0008
Nuclear Periphery 0.0194 0.0032 0.0028 0.0017 0.0031 0.0039 0.0039 0.0015 0.0021 0.0033 0.0022 0.0016 0.0709 0.0008 0.0036 0.0097 0.0022 0.0037
Nucleolus 0.0005 0 0 0.0002 0 0 0.0001 0 0.0001 0 0 0.0001 0.0006 0 0.0001 0.0002 0 0
Nucleus 0.0178 0.0022 0.0012 0.0021 0.0017 0.0025 0.0018 0.0018 0.0026 0.0019 0.002 0.0016 0.019 0.0008 0.0011 0.0008 0.0022 0.0012
Peroxisomes 0.004 0.0005 0.002 0.0013 0.0005 0.0006 0.0014 0.0005 0.001 0.0016 0.0002 0.0004 0.0019 0.001 0.0236 0.0008 0.0009 0.0006
Punctate Nuclear 0.002 0.0005 0.0004 0.0003 0.0004 0.0004 0.0005 0.0004 0.001 0.0003 0.0003 0.0002 0.0087 0.0001 0.0005 0.0004 0.0005 0.0007
Vacuole 0.0071 0.0015 0.0035 0.0049 0.0062 0.0043 0.0039 0.0032 0.0043 0.0168 0.0031 0.0023 0.0033 0.0013 0.004 0.0029 0.0027 0.0019
Vacuole Periphery 0.0037 0.0004 0.0014 0.0021 0.0037 0.0023 0.0011 0.0004 0.001 0.004 0.0015 0.0009 0.0037 0.0005 0.0036 0.0051 0.0019 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 93.2528 99.6622 76.0271 86.4004 102.669 85.391 84.7584 96.9327 97.7124 99.2665
Translational Efficiency 2.2115 1.7051 2.107 1.7504 1.9191 2.4823 1.842 1.791 1.6995 1.8822

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; required for prospore membrane formation; regulates phospholipase Spo14p; shares functional similarity with Glo3p; GCS1 has a paralog, SPS18, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (90%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Gcs1

Gcs1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gcs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available