Standard name
Human Ortholog
Description Phosphotyrosine-specific protein phosphatase; dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Cytoplasm 0.98 1.0 0.98 1.0 0.99 0.95 0.9 0.89 0.82 0.83 0.77 0.86 0.96 0.96 0.98 0.93 0.95 0.95 0.96 0.87 0.91 0.94 0.87 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0.17 0.16 0.26 0.24 0.36 0.19 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 4
Bud 0 0 0 0 1 2 6 0 10 7 9 5 0 1 0 0 0 0 0 0 0 2 6 14
Bud Neck 0 0 1 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 1 1 3 4 4 4 5 2 5 7 1 1 5 22 8 8 0 0 0 0 0 1
Cytoplasm 192 70 182 178 295 247 323 304 254 270 176 237 371 577 580 359 160 193 185 68 170 268 349 377
Endoplasmic Reticulum 1 0 0 0 0 1 2 0 2 0 5 2 0 3 0 7 4 6 0 1 1 0 1 1
Endosome 0 0 0 0 0 0 0 2 0 1 0 0 3 1 1 1 1 0 1 1 1 1 0 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 2 0 0 1 0 0 2 0
Mitochondria 8 0 0 0 5 24 59 53 81 79 81 52 3 0 0 1 0 3 3 2 6 3 6 6
Nucleus 1 1 1 0 0 1 2 3 3 1 1 1 12 24 7 9 2 1 0 0 0 5 13 4
Nuclear Periphery 0 0 0 0 0 0 0 2 0 2 0 0 0 0 1 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 1 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 4 3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
SpindlePole 0 0 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 3 5
Vac/Vac Membrane 1 0 1 0 1 3 12 14 3 6 1 5 1 0 0 33 11 6 0 0 0 0 2 1
Unique Cell Count 195 70 185 178 298 260 357 340 309 326 228 277 387 604 591 385 168 203 193 79 188 287 403 441
Labelled Cell Count 204 72 187 179 308 282 408 382 358 368 279 309 393 610 597 433 188 217 193 79 188 287 403 441


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 5.8 5.4 5.1 5.7 4.9 4.7 5.4 4.4 4.6 4.1 4.6 5.6 6.2 6.2 12.3 12.1 11.8 5.7 6.0 6.4
Std Deviation (1e-4) 0.6 1.2 1.2 1.0 1.6 0.9 1.3 1.6 0.8 0.9 0.8 0.6 1.1 1.5 1.2 2.3 2.0 2.2 1.8 1.8 2.0
Intensity Change (Log2) – – – -0.08 0.06 -0.16 -0.2 -0.01 -0.3 -0.25 -0.39 -0.24 0.06 0.2 0.19 1.19 1.16 1.13 0.07 0.16 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.9 0 0
Cytoplasm 1.7 0.6 -1.9 -3.5 -3.7 -5.4 -5.3 -6.3 -4.6 -1.6 -1.8 -0.2 -2.6 -1.7 -1.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 5.7 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 2.3 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.4 0 0 0 0 0 0 0 3.8 3.1 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5085 0.5612 0.2603 0.1744 -0.0321 -0.0205 0.7793 1.0129 0.696 0.8847 2.818 0.9859 -0.0584 0.5698 0.1439 0.6878 0.0787 0.1812
Actin 0.0256 0.0012 0.0138 0.005 0.0262 0.0015 0.0229 0.0003 0.0057 0.0028 0.071 0.0146 0.0195 0.0004 0.0216 0.1013 0.0029 0.0111
Bud 0.0009 0.0053 0.0046 0 0.0005 0.0002 0.0024 0.0012 0.0022 0.0005 0.0059 0.0046 0.0009 0.0073 0.0017 0.0041 0.0002 0.0046
Bud Neck 0.0009 0.0001 0.0002 0.0002 0.0005 0.0005 0.0241 0.0001 0.0007 0.0004 0.008 0.0053 0.003 0.0016 0.0003 0.0011 0.0002 0.0016
Bud Periphery 0.003 0.0042 0.002 0 0.001 0.0003 0.004 0.0013 0.0017 0.001 0.0074 0.0111 0.001 0.0011 0.0022 0.0031 0.0002 0.0118
Bud Site 0.009 0.0073 0.0008 0.0002 0.0012 0.0001 0.0079 0.0053 0.0097 0.0009 0.0154 0.0013 0.0093 0.024 0.0015 0.0018 0.0002 0.0012
Cell Periphery 0.0015 0.0002 0.0001 0 0.0002 0.0001 0.0006 0.0002 0.0002 0.0002 0.0017 0.0002 0.0004 0.0007 0.0001 0.0002 0.0001 0.0002
Cytoplasm 0.2219 0.3648 0.3878 0.268 0.486 0.4632 0.235 0.5179 0.4496 0.6897 0.1939 0.5713 0.4923 0.6216 0.5969 0.5922 0.5621 0.6933
Cytoplasmic Foci 0.0139 0.0047 0.0179 0.0093 0.0139 0.0216 0.0671 0.0043 0.015 0.0134 0.0453 0.0293 0.0239 0.0097 0.0181 0.0204 0.0245 0.0204
Eisosomes 0.0009 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0 0.0001 0.0001 0.001 0.0001 0.0004 0 0.0001 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.0157 0.0035 0.0016 0.0016 0.0027 0.003 0.0033 0.0011 0.0039 0.0017 0.005 0.0021 0.0066 0.0024 0.0041 0.0021 0.0039 0.0025
Endosome 0.0278 0.0022 0.0024 0.0019 0.0311 0.0106 0.0313 0.0007 0.0114 0.029 0.1109 0.0096 0.0191 0.0072 0.0233 0.0148 0.0431 0.0104
Golgi 0.0102 0.0002 0.0012 0.0012 0.0097 0.001 0.0121 0.0001 0.0037 0.0089 0.0546 0.0055 0.0093 0.0027 0.0114 0.032 0.0189 0.0043
Lipid Particles 0.0063 0.0005 0.0042 0.0032 0.0082 0.0057 0.0177 0.0001 0.0049 0.005 0.0392 0.0038 0.0155 0.0007 0.0054 0.0428 0.0032 0.005
Mitochondria 0.0143 0.0007 0.0324 0.0015 0.0099 0.0007 0.0091 0.0002 0.0173 0.0172 0.0462 0.0055 0.0078 0.0005 0.0017 0.0138 0.0028 0.0028
None 0.6 0.5979 0.513 0.6535 0.3557 0.4765 0.5064 0.4639 0.4503 0.2196 0.2951 0.3199 0.3697 0.3119 0.291 0.1222 0.3284 0.2086
Nuclear Periphery 0.0307 0.0012 0.0009 0.001 0.0016 0.0008 0.0021 0.0004 0.0021 0.0005 0.0073 0.0015 0.0037 0.0007 0.0013 0.0015 0.0022 0.0073
Nucleolus 0.0003 0.0006 0.0006 0.0002 0.0007 0.0002 0.0026 0.0001 0.0007 0.0002 0.0156 0.0002 0.0006 0.0008 0.0001 0.0003 0.0001 0.001
Nucleus 0.0045 0.0021 0.0018 0.0039 0.0021 0.0025 0.0026 0.0015 0.0038 0.0015 0.0095 0.0038 0.0034 0.0026 0.0011 0.0009 0.0014 0.0029
Peroxisomes 0.0032 0.0002 0.0078 0.0437 0.0195 0.0078 0.022 0.0001 0.0092 0.0023 0.0066 0.0061 0.0065 0.0013 0.0154 0.0423 0.0018 0.0052
Punctate Nuclear 0.0014 0.0012 0.0052 0.0048 0.0009 0.0022 0.0203 0.0005 0.0061 0.0004 0.0186 0.0025 0.0041 0.0008 0.0009 0.0014 0.0016 0.0037
Vacuole 0.0047 0.0014 0.0013 0.0005 0.0186 0.0014 0.0049 0.0007 0.0013 0.0035 0.0317 0.0014 0.0026 0.002 0.0014 0.0008 0.0016 0.0013
Vacuole Periphery 0.0032 0.0003 0.0005 0.0001 0.0097 0.0003 0.0011 0.0001 0.0004 0.0012 0.01 0.0003 0.0005 0.0002 0.0004 0.0007 0.0008 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.3251 15.6568 7.2612 14.6041 10.6444 6.5642 15.0885 13.6896 14.2917 13.9673
Translational Efficiency 0.6971 0.7482 1.5664 0.7736 1.3823 2.2269 0.8351 1.0722 1.0728 0.8507

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
556 566 2838 777 1900 1565 1994 1060 2456 2131 4832 1837

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 725.97 832.69 899.76 982.78 706.07 774.73 991.97 952.86 710.58 790.12 937.81 965.52
Standard Deviation 83.58 456.52 101.26 138.00 79.50 90.28 125.09 504.75 80.87 248.99 120.58 394.06
Intensity Change Log 2 — 0.197870 0.309630 0.436959 — 0.133882 0.490485 0.432453 — 0.166675 0.401634 0.434739

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000126 0.000318 0.000133 0.000864 0.000069 0.000292 0.000172 0.000856 0.000082 0.000299 0.000149 0.000859
Bud Neck 0.015884 0.016442 0.004175 0.005221 0.010627 0.022132 0.002092 0.003769 0.011817 0.020621 0.003316 0.004383
Bud Site 0.004218 0.014083 0.010559 0.017136 0.004055 0.019647 0.006462 0.020951 0.004092 0.018169 0.008868 0.019337
Cell Periphery 0.000116 0.000092 0.000072 0.000127 0.000105 0.000097 0.000094 0.000217 0.000107 0.000096 0.000081 0.000179
Cytoplasm 0.506309* 0.521636* 0.601540* 0.477531* 0.576547* 0.579839* 0.598765* 0.561744* 0.560646* 0.564380* 0.600395* 0.526124*
Cytoplasmic Foci 0.236268* 0.204271* 0.005905 0.028244 0.164745 0.164935 0.010192 0.033696 0.180937* 0.175382 0.007674 0.031390
Eisosomes 0.000121 0.000072 0.000014 0.000050 0.000095 0.000073 0.000024 0.000046 0.000101 0.000073 0.000018 0.000048
Endoplasmic Reticulum 0.000549 0.000886 0.000873 0.002806 0.000649 0.000633 0.001581 0.001093 0.000626 0.000701 0.001165 0.001818
Endosome 0.017906 0.020226 0.000696 0.011683 0.006823 0.013510 0.001320 0.014575 0.009332 0.015293 0.000954 0.013352
Golgi 0.000979 0.001696 0.000036 0.018349 0.001067 0.003757 0.000056 0.012520 0.001047 0.003210 0.000044 0.014985
Lipid Particles 0.004953 0.003440 0.000328 0.001838 0.004161 0.002114 0.000662 0.003753 0.004340 0.002467 0.000466 0.002943
Mitochondria 0.004816 0.001236 0.000696 0.001430 0.002506 0.002907 0.000334 0.009993 0.003029 0.002463 0.000546 0.006371
Mitotic Spindle 0.002830 0.005265 0.002166 0.061127 0.002125 0.006978 0.004177 0.032484 0.002284 0.006523 0.002996 0.044599
None 0.005469 0.002229 0.004097 0.003045 0.002828 0.002453 0.006729 0.008261 0.003426 0.002393 0.005183 0.006055
Nuclear Periphery 0.000370 0.000394 0.001036 0.001992 0.000339 0.000266 0.001008 0.000703 0.000346 0.000300 0.001024 0.001248
Nuclear Periphery Foci 0.000489 0.000961 0.000384 0.009055 0.000286 0.000471 0.001507 0.002231 0.000332 0.000601 0.000847 0.005117
Nucleolus 0.000885 0.001540 0.000162 0.000320 0.000656 0.000838 0.000165 0.001378 0.000708 0.001025 0.000163 0.000931
Nucleus 0.122331 0.084718 0.331209* 0.096521 0.168074* 0.091865 0.299199* 0.107782 0.157719* 0.089966 0.318000* 0.103019
Peroxisomes 0.004574 0.009542 0.000060 0.000873 0.001750 0.006966 0.000324 0.002450 0.002389 0.007650 0.000169 0.001783
Vacuole 0.069933 0.109791 0.035764 0.257676* 0.051990 0.079051 0.065059 0.178769 0.056052 0.087216 0.047853 0.212145*
Vacuole Periphery 0.000877 0.001165 0.000094 0.004114 0.000503 0.001175 0.000079 0.002731 0.000588 0.001172 0.000088 0.003316

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.74 -0.55 -4.70 -1.39 -4.27 -7.58 -6.96 -2.83 -1.09 -1.76 -8.59 -4.26 -4.42 -1.58 -3.17
Bud Neck -0.25 4.62 4.98 6.32 1.30 -5.72 9.47 9.06 10.87 -0.19 -5.22 9.21 10.00 12.39 1.49
Bud Site -6.30 -3.03 -3.43 1.91 -0.90 -8.27 -0.74 -4.00 3.58 -3.50 -9.67 -2.98 -5.18 4.26 -2.77
Cell Periphery 2.99 5.86 1.79 -1.62 -5.03 0.99 2.36 -0.10 -0.46 -0.82 1.89 5.22 0.19 -0.75 -2.08
Cytoplasm -0.41 -2.14 6.65 6.80 11.34 0.11 11.20 10.08 9.23 1.52 0.22 7.57 14.01 12.73 9.05
Cytoplasmic Foci 2.90 26.54 25.24 21.33 -10.55 -0.06 37.83 33.97 30.29 -7.26 1.14 45.95 42.23 37.28 -11.86
Eisosomes 6.12 15.25 10.36 4.99 -15.00 3.91 12.47 10.23 8.89 -5.45 5.93 18.54 13.07 9.89 -15.70
Endoplasmic Reticulum -2.67 -8.06 -8.31 -5.39 -3.60 0.09 -14.33 -3.83 -2.18 10.51 -0.80 -16.37 -8.94 -5.20 4.03
Endosome -0.66 7.08 5.79 8.49 -4.15 -5.98 11.83 1.84 6.24 -3.92 -5.29 12.70 5.69 10.16 -5.54
Golgi -2.53 7.53 -1.41 -1.00 -1.98 -3.94 6.17 -2.02 0.14 -2.90 -4.23 7.72 -2.40 -0.25 -3.45
Lipid Particles 2.49 9.27 6.91 4.63 -12.26 5.15 10.08 4.06 0.75 -1.60 5.57 13.49 6.67 2.29 -3.35
Mitochondria 1.61 1.84 1.91 2.70 0.54 -0.41 3.44 -0.34 -0.06 -2.57 0.78 3.51 1.23 0.72 -2.31
Mitotic Spindle -0.91 0.21 -5.25 -4.70 -5.71 -3.02 -2.43 -5.29 -3.46 -4.25 -3.10 -1.47 -7.60 -5.71 -7.30
None 1.82 0.70 0.77 -3.56 0.40 0.58 -5.30 -2.03 -2.43 0.87 1.59 -2.66 -1.53 -3.29 0.46
Nuclear Periphery -0.87 -17.43 -6.85 -6.50 -0.48 1.14 -26.67 -12.49 -10.13 9.57 0.63 -34.62 -11.31 -10.64 3.75
Nuclear Periphery Foci -3.88 -0.77 -5.31 -4.42 -5.24 -2.97 -6.63 -5.37 -4.37 1.22 -4.88 -7.35 -7.17 -6.14 -4.50
Nucleolus -2.26 5.54 5.17 4.74 -1.07 -1.65 8.48 0.66 1.46 -1.58 -2.82 10.00 2.44 4.05 -1.72
Nucleus 4.95 -33.39 -1.47 -7.09 35.00 15.85 -29.00 7.06 -6.51 32.97 16.51 -44.18 5.96 -9.50 48.86
Peroxisomes -3.36 6.50 6.18 7.23 -3.77 -8.47 8.84 2.81 9.12 -1.71 -8.91 11.54 6.85 12.09 -2.34
Vacuole -6.33 2.38 -26.11 -20.23 -29.24 -9.05 -15.51 -30.24 -25.84 -21.05 -11.30 -10.36 -41.22 -34.21 -36.56
Vacuole Periphery -1.29 6.10 0.19 0.88 -2.29 -3.70 11.80 -1.52 1.34 -3.78 -4.05 12.35 -1.22 1.54 -4.18
Standard name
Human Ortholog
Description Phosphotyrosine-specific protein phosphatase; dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation
Localization
Cell Percentages cytoplasm (69%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ptp1

Ptp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ptp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available