Standard name | |
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Human Ortholog | |
Description | Phosphotyrosine-specific protein phosphatase; dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 4 |
Bud | 0 | 0 | 0 | 0 | 1 | 2 | 6 | 0 | 10 | 7 | 9 | 5 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 6 | 14 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 1 | 1 | 1 | 3 | 4 | 4 | 4 | 5 | 2 | 5 | 7 | 1 | 1 | 5 | 22 | 8 | 8 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 192 | 70 | 182 | 178 | 295 | 247 | 323 | 304 | 254 | 270 | 176 | 237 | 371 | 577 | 580 | 359 | 160 | 193 | 185 | 68 | 170 | 268 | 349 | 377 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 5 | 2 | 0 | 3 | 0 | 7 | 4 | 6 | 0 | 1 | 1 | 0 | 1 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 3 | 1 | 1 | 1 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 2 | 0 |
Mitochondria | 8 | 0 | 0 | 0 | 5 | 24 | 59 | 53 | 81 | 79 | 81 | 52 | 3 | 0 | 0 | 1 | 0 | 3 | 3 | 2 | 6 | 3 | 6 | 6 |
Nucleus | 1 | 1 | 1 | 0 | 0 | 1 | 2 | 3 | 3 | 1 | 1 | 1 | 12 | 24 | 7 | 9 | 2 | 1 | 0 | 0 | 0 | 5 | 13 | 4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 5 |
Vac/Vac Membrane | 1 | 0 | 1 | 0 | 1 | 3 | 12 | 14 | 3 | 6 | 1 | 5 | 1 | 0 | 0 | 33 | 11 | 6 | 0 | 0 | 0 | 0 | 2 | 1 |
Unique Cell Count | 195 | 70 | 185 | 178 | 298 | 260 | 357 | 340 | 309 | 326 | 228 | 277 | 387 | 604 | 591 | 385 | 168 | 203 | 193 | 79 | 188 | 287 | 403 | 441 |
Labelled Cell Count | 204 | 72 | 187 | 179 | 308 | 282 | 408 | 382 | 358 | 368 | 279 | 309 | 393 | 610 | 597 | 433 | 188 | 217 | 193 | 79 | 188 | 287 | 403 | 441 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 5.8 | 5.4 | 5.1 | 5.7 | 4.9 | 4.7 | 5.4 | 4.4 | 4.6 | 4.1 | 4.6 | 5.6 | 6.2 | 6.2 | 12.3 | 12.1 | 11.8 | 5.7 | 6.0 | 6.4 |
Std Deviation (1e-4) | 0.6 | 1.2 | 1.2 | 1.0 | 1.6 | 0.9 | 1.3 | 1.6 | 0.8 | 0.9 | 0.8 | 0.6 | 1.1 | 1.5 | 1.2 | 2.3 | 2.0 | 2.2 | 1.8 | 1.8 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.06 | -0.16 | -0.2 | -0.01 | -0.3 | -0.25 | -0.39 | -0.24 | 0.06 | 0.2 | 0.19 | 1.19 | 1.16 | 1.13 | 0.07 | 0.16 | 0.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 | 0 |
Cytoplasm | 1.7 | 0.6 | -1.9 | -3.5 | -3.7 | -5.4 | -5.3 | -6.3 | -4.6 | -1.6 | -1.8 | -0.2 | -2.6 | -1.7 | -1.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 5.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.8 | 3.1 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.5085 | 0.5612 | 0.2603 | 0.1744 | -0.0321 | -0.0205 | 0.7793 | 1.0129 | 0.696 | 0.8847 | 2.818 | 0.9859 | -0.0584 | 0.5698 | 0.1439 | 0.6878 | 0.0787 | 0.1812 |
Actin | 0.0256 | 0.0012 | 0.0138 | 0.005 | 0.0262 | 0.0015 | 0.0229 | 0.0003 | 0.0057 | 0.0028 | 0.071 | 0.0146 | 0.0195 | 0.0004 | 0.0216 | 0.1013 | 0.0029 | 0.0111 |
Bud | 0.0009 | 0.0053 | 0.0046 | 0 | 0.0005 | 0.0002 | 0.0024 | 0.0012 | 0.0022 | 0.0005 | 0.0059 | 0.0046 | 0.0009 | 0.0073 | 0.0017 | 0.0041 | 0.0002 | 0.0046 |
Bud Neck | 0.0009 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0005 | 0.0241 | 0.0001 | 0.0007 | 0.0004 | 0.008 | 0.0053 | 0.003 | 0.0016 | 0.0003 | 0.0011 | 0.0002 | 0.0016 |
Bud Periphery | 0.003 | 0.0042 | 0.002 | 0 | 0.001 | 0.0003 | 0.004 | 0.0013 | 0.0017 | 0.001 | 0.0074 | 0.0111 | 0.001 | 0.0011 | 0.0022 | 0.0031 | 0.0002 | 0.0118 |
Bud Site | 0.009 | 0.0073 | 0.0008 | 0.0002 | 0.0012 | 0.0001 | 0.0079 | 0.0053 | 0.0097 | 0.0009 | 0.0154 | 0.0013 | 0.0093 | 0.024 | 0.0015 | 0.0018 | 0.0002 | 0.0012 |
Cell Periphery | 0.0015 | 0.0002 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0017 | 0.0002 | 0.0004 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0002 |
Cytoplasm | 0.2219 | 0.3648 | 0.3878 | 0.268 | 0.486 | 0.4632 | 0.235 | 0.5179 | 0.4496 | 0.6897 | 0.1939 | 0.5713 | 0.4923 | 0.6216 | 0.5969 | 0.5922 | 0.5621 | 0.6933 |
Cytoplasmic Foci | 0.0139 | 0.0047 | 0.0179 | 0.0093 | 0.0139 | 0.0216 | 0.0671 | 0.0043 | 0.015 | 0.0134 | 0.0453 | 0.0293 | 0.0239 | 0.0097 | 0.0181 | 0.0204 | 0.0245 | 0.0204 |
Eisosomes | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0157 | 0.0035 | 0.0016 | 0.0016 | 0.0027 | 0.003 | 0.0033 | 0.0011 | 0.0039 | 0.0017 | 0.005 | 0.0021 | 0.0066 | 0.0024 | 0.0041 | 0.0021 | 0.0039 | 0.0025 |
Endosome | 0.0278 | 0.0022 | 0.0024 | 0.0019 | 0.0311 | 0.0106 | 0.0313 | 0.0007 | 0.0114 | 0.029 | 0.1109 | 0.0096 | 0.0191 | 0.0072 | 0.0233 | 0.0148 | 0.0431 | 0.0104 |
Golgi | 0.0102 | 0.0002 | 0.0012 | 0.0012 | 0.0097 | 0.001 | 0.0121 | 0.0001 | 0.0037 | 0.0089 | 0.0546 | 0.0055 | 0.0093 | 0.0027 | 0.0114 | 0.032 | 0.0189 | 0.0043 |
Lipid Particles | 0.0063 | 0.0005 | 0.0042 | 0.0032 | 0.0082 | 0.0057 | 0.0177 | 0.0001 | 0.0049 | 0.005 | 0.0392 | 0.0038 | 0.0155 | 0.0007 | 0.0054 | 0.0428 | 0.0032 | 0.005 |
Mitochondria | 0.0143 | 0.0007 | 0.0324 | 0.0015 | 0.0099 | 0.0007 | 0.0091 | 0.0002 | 0.0173 | 0.0172 | 0.0462 | 0.0055 | 0.0078 | 0.0005 | 0.0017 | 0.0138 | 0.0028 | 0.0028 |
None | 0.6 | 0.5979 | 0.513 | 0.6535 | 0.3557 | 0.4765 | 0.5064 | 0.4639 | 0.4503 | 0.2196 | 0.2951 | 0.3199 | 0.3697 | 0.3119 | 0.291 | 0.1222 | 0.3284 | 0.2086 |
Nuclear Periphery | 0.0307 | 0.0012 | 0.0009 | 0.001 | 0.0016 | 0.0008 | 0.0021 | 0.0004 | 0.0021 | 0.0005 | 0.0073 | 0.0015 | 0.0037 | 0.0007 | 0.0013 | 0.0015 | 0.0022 | 0.0073 |
Nucleolus | 0.0003 | 0.0006 | 0.0006 | 0.0002 | 0.0007 | 0.0002 | 0.0026 | 0.0001 | 0.0007 | 0.0002 | 0.0156 | 0.0002 | 0.0006 | 0.0008 | 0.0001 | 0.0003 | 0.0001 | 0.001 |
Nucleus | 0.0045 | 0.0021 | 0.0018 | 0.0039 | 0.0021 | 0.0025 | 0.0026 | 0.0015 | 0.0038 | 0.0015 | 0.0095 | 0.0038 | 0.0034 | 0.0026 | 0.0011 | 0.0009 | 0.0014 | 0.0029 |
Peroxisomes | 0.0032 | 0.0002 | 0.0078 | 0.0437 | 0.0195 | 0.0078 | 0.022 | 0.0001 | 0.0092 | 0.0023 | 0.0066 | 0.0061 | 0.0065 | 0.0013 | 0.0154 | 0.0423 | 0.0018 | 0.0052 |
Punctate Nuclear | 0.0014 | 0.0012 | 0.0052 | 0.0048 | 0.0009 | 0.0022 | 0.0203 | 0.0005 | 0.0061 | 0.0004 | 0.0186 | 0.0025 | 0.0041 | 0.0008 | 0.0009 | 0.0014 | 0.0016 | 0.0037 |
Vacuole | 0.0047 | 0.0014 | 0.0013 | 0.0005 | 0.0186 | 0.0014 | 0.0049 | 0.0007 | 0.0013 | 0.0035 | 0.0317 | 0.0014 | 0.0026 | 0.002 | 0.0014 | 0.0008 | 0.0016 | 0.0013 |
Vacuole Periphery | 0.0032 | 0.0003 | 0.0005 | 0.0001 | 0.0097 | 0.0003 | 0.0011 | 0.0001 | 0.0004 | 0.0012 | 0.01 | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0.0007 | 0.0008 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.3251 | 15.6568 | 7.2612 | 14.6041 | 10.6444 | 6.5642 | 15.0885 | 13.6896 | 14.2917 | 13.9673 |
Translational Efficiency | 0.6971 | 0.7482 | 1.5664 | 0.7736 | 1.3823 | 2.2269 | 0.8351 | 1.0722 | 1.0728 | 0.8507 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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