Standard name | |
---|---|
Human Ortholog | |
Description | GTPase-activating protein for yeast Rab family members; members include Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking; contains a PH-like domain |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 1 | 5 | 5 | 4 | 5 | 15 | 11 | 2 | 6 | 2 | 9 | 1 | 0 | 1 | 1 | 6 | 1 | 3 | 1 | 3 | 2 | 3 | 6 |
Bud | 0 | 7 | 7 | 1 | 2 | 1 | 13 | 5 | 12 | 12 | 10 | 10 | 3 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 3 | 9 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 3 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 11 | 1 | 4 | 8 | 3 | 4 | 5 | 10 | 4 | 7 | 1 | 2 | 2 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 121 | 125 | 275 | 256 | 315 | 313 | 278 | 308 | 158 | 224 | 119 | 163 | 167 | 271 | 261 | 92 | 68 | 19 | 115 | 132 | 267 | 90 | 169 | 218 |
Endoplasmic Reticulum | 2 | 5 | 4 | 5 | 8 | 13 | 20 | 11 | 11 | 15 | 13 | 12 | 5 | 2 | 5 | 6 | 3 | 0 | 2 | 0 | 2 | 2 | 9 | 6 |
Endosome | 8 | 3 | 4 | 3 | 8 | 6 | 13 | 23 | 10 | 7 | 1 | 15 | 7 | 10 | 11 | 10 | 10 | 1 | 11 | 9 | 9 | 1 | 5 | 9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 5 | 1 | 0 | 0 | 1 | 0 | 2 | 2 |
Mitochondria | 2 | 4 | 1 | 1 | 5 | 26 | 58 | 29 | 116 | 110 | 133 | 99 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 6 | 17 | 3 | 7 | 4 |
Nucleus | 0 | 1 | 0 | 2 | 1 | 1 | 4 | 7 | 4 | 3 | 4 | 9 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 3 | 0 | 4 | 1 | 4 | 2 | 8 | 9 | 2 | 6 | 0 | 5 | 2 | 1 | 1 | 5 | 6 | 0 | 3 | 5 | 5 | 2 | 7 | 7 |
Vac/Vac Membrane | 0 | 4 | 2 | 3 | 5 | 13 | 22 | 99 | 54 | 61 | 25 | 36 | 1 | 9 | 1 | 16 | 15 | 6 | 0 | 3 | 7 | 4 | 8 | 4 |
Unique Cell Count | 136 | 145 | 294 | 269 | 340 | 362 | 389 | 454 | 315 | 392 | 268 | 312 | 183 | 289 | 278 | 120 | 99 | 25 | 143 | 167 | 322 | 114 | 227 | 280 |
Labelled Cell Count | 144 | 152 | 314 | 278 | 356 | 388 | 435 | 509 | 382 | 457 | 316 | 367 | 189 | 297 | 285 | 141 | 118 | 30 | 143 | 167 | 322 | 114 | 227 | 280 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.0 | 6.0 | 6.6 | 6.4 | 7.2 | 6.0 | 6.0 | 6.8 | 5.6 | 5.6 | 5.3 | 5.8 | 6.9 | 7.4 | 7.4 | 11.7 | 12.1 | 11.3 | 6.1 | 6.9 | 8.1 |
Std Deviation (1e-4) | 1.0 | 1.5 | 0.9 | 1.1 | 1.1 | 1.1 | 1.2 | 1.2 | 1.3 | 1.2 | 1.2 | 1.1 | 1.0 | 1.1 | 1.3 | 4.8 | 2.8 | 1.9 | 1.1 | 1.6 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.11 | -0.14 | -0.14 | 0.03 | -0.23 | -0.25 | -0.33 | -0.2 | 0.05 | 0.15 | 0.15 | 0.82 | 0.86 | 0.77 | -0.12 | 0.06 | 0.29 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 1.7 | 0.7 | 0 | 0 | 0 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0.7 | 0 | 1.0 | 0.5 | 0.9 | 0.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.8 | -2.1 | -1.2 | -2.7 | -2.7 | -1.7 | -0.9 | -1.7 | -1.1 | 0 | -2.5 | -2.4 | 0 | 0 | 0 |
Cytoplasm | 0.8 | -0.4 | -3.0 | -7.3 | -8.3 | -11.8 | -10.6 | -12.7 | -11.3 | -0.9 | 0.1 | 0.2 | -4.9 | -6.4 | -3.1 |
Endoplasmic Reticulum | 0 | 0.9 | 1.8 | 2.7 | 1.0 | 1.7 | 1.9 | 2.4 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0.9 | 0 | 1.6 | 2.7 | 1.5 | 0 | 0 | 2.4 | 0 | 1.7 | 1.9 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.4 | 6.7 | 4.1 | 11.4 | 9.8 | 13.7 | 10.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0.7 | 0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 2.5 | 3.5 | 8.3 | 7.0 | 6.7 | 4.8 | 5.5 | 0 | 2.2 | 0 | 5.7 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.8532 | 2.2601 | 1.7421 | 1.388 | 1.9702 | 1.9564 | 1.9324 | 2.4708 | 2.1566 | 1.938 | 1.2574 | 2.2513 | 1.8021 | 2.7028 | 1.9979 | 2.1095 | 1.4947 | 2.1286 |
Actin | 0.0394 | 0.0079 | 0.0286 | 0.0362 | 0.0508 | 0.0351 | 0.0184 | 0.014 | 0.0158 | 0.0077 | 0.0001 | 0.0021 | 0.0239 | 0.0137 | 0.0051 | 0.0013 | 0.0058 | 0.0079 |
Bud | 0.005 | 0.0015 | 0.0008 | 0.0022 | 0.001 | 0.0009 | 0.0005 | 0.0012 | 0.0017 | 0.0003 | 0.0001 | 0.0001 | 0.0006 | 0.0007 | 0.0006 | 0.0003 | 0.0005 | 0.0001 |
Bud Neck | 0.0025 | 0.0039 | 0.0183 | 0.0019 | 0.0029 | 0.0242 | 0.0006 | 0.0004 | 0.0028 | 0.0005 | 0.0004 | 0.0031 | 0.0014 | 0.0003 | 0.0004 | 0.0003 | 0.0005 | 0.0013 |
Bud Periphery | 0.0029 | 0.0011 | 0.0006 | 0.0018 | 0.0012 | 0.0008 | 0.0004 | 0.0006 | 0.0064 | 0.0002 | 0 | 0 | 0.0005 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Bud Site | 0.0067 | 0.0115 | 0.0081 | 0.02 | 0.0048 | 0.0019 | 0.002 | 0.0077 | 0.0051 | 0.0007 | 0.0001 | 0.0001 | 0.0032 | 0.0118 | 0.0009 | 0.0003 | 0.0003 | 0.0001 |
Cell Periphery | 0.0006 | 0.0003 | 0.0004 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0006 | 0 | 0 | 0.0001 | 0.0008 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.5258 | 0.7563 | 0.6351 | 0.5887 | 0.5332 | 0.6738 | 0.6869 | 0.8892 | 0.8259 | 0.7931 | 0.9736 | 0.8571 | 0.7278 | 0.8589 | 0.7752 | 0.675 | 0.8055 | 0.8134 |
Cytoplasmic Foci | 0.1227 | 0.1078 | 0.1175 | 0.0883 | 0.064 | 0.1057 | 0.0903 | 0.0256 | 0.037 | 0.1099 | 0.0067 | 0.0696 | 0.0797 | 0.0407 | 0.1118 | 0.1338 | 0.0353 | 0.061 |
Eisosomes | 0.0004 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0121 | 0.0053 | 0.0051 | 0.0148 | 0.0102 | 0.0058 | 0.0086 | 0.0026 | 0.0024 | 0.0025 | 0.002 | 0.0032 | 0.0144 | 0.0028 | 0.0063 | 0.0043 | 0.0081 | 0.0041 |
Endosome | 0.0511 | 0.0178 | 0.0391 | 0.0221 | 0.0742 | 0.0306 | 0.0346 | 0.0091 | 0.0095 | 0.0275 | 0.0032 | 0.0201 | 0.0267 | 0.0132 | 0.0315 | 0.0437 | 0.0373 | 0.0147 |
Golgi | 0.0141 | 0.0061 | 0.009 | 0.0128 | 0.0246 | 0.0086 | 0.0221 | 0.0029 | 0.0046 | 0.0086 | 0.0001 | 0.0016 | 0.0051 | 0.0063 | 0.0055 | 0.0196 | 0.0281 | 0.003 |
Lipid Particles | 0.0183 | 0.0018 | 0.0162 | 0.0056 | 0.0142 | 0.0071 | 0.0102 | 0.0002 | 0.0025 | 0.0082 | 0 | 0.0003 | 0.0101 | 0.0008 | 0.0019 | 0.0035 | 0.0032 | 0.0113 |
Mitochondria | 0.0103 | 0.0018 | 0.0202 | 0.0132 | 0.0645 | 0.0024 | 0.0087 | 0.0005 | 0.0073 | 0.0011 | 0.0001 | 0.0003 | 0.0012 | 0.0068 | 0.0125 | 0.0004 | 0.011 | 0.0049 |
None | 0.1096 | 0.055 | 0.0734 | 0.1078 | 0.0141 | 0.0602 | 0.0906 | 0.0399 | 0.0715 | 0.0216 | 0.0099 | 0.0352 | 0.0703 | 0.024 | 0.0396 | 0.1086 | 0.0222 | 0.0578 |
Nuclear Periphery | 0.0207 | 0.0013 | 0.0033 | 0.0028 | 0.0382 | 0.0023 | 0.0037 | 0.0007 | 0.0006 | 0.0011 | 0.0005 | 0.0003 | 0.0125 | 0.0006 | 0.0011 | 0.0011 | 0.0016 | 0.0006 |
Nucleolus | 0.0029 | 0.0004 | 0.001 | 0.0003 | 0.0011 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 |
Nucleus | 0.0213 | 0.0029 | 0.0025 | 0.0033 | 0.0207 | 0.0077 | 0.0017 | 0.0014 | 0.0011 | 0.001 | 0.0021 | 0.0008 | 0.0029 | 0.0008 | 0.0012 | 0.0016 | 0.0016 | 0.0016 |
Peroxisomes | 0.0151 | 0.011 | 0.0148 | 0.0691 | 0.0545 | 0.0068 | 0.0122 | 0.0009 | 0.0023 | 0.0109 | 0 | 0.0043 | 0.0091 | 0.0164 | 0.0034 | 0.0036 | 0.0357 | 0.0036 |
Punctate Nuclear | 0.0126 | 0.0042 | 0.0022 | 0.0065 | 0.0135 | 0.0226 | 0.0059 | 0.0008 | 0.0013 | 0.004 | 0.0003 | 0.0007 | 0.0058 | 0.0005 | 0.0007 | 0.0009 | 0.0006 | 0.0137 |
Vacuole | 0.004 | 0.0019 | 0.002 | 0.0019 | 0.0079 | 0.0021 | 0.0014 | 0.0018 | 0.0013 | 0.0008 | 0.0006 | 0.0007 | 0.0024 | 0.0007 | 0.0016 | 0.0012 | 0.0017 | 0.0005 |
Vacuole Periphery | 0.0018 | 0.0003 | 0.0012 | 0.0004 | 0.0038 | 0.0004 | 0.0007 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0012 | 0.0003 | 0.0003 | 0.0003 | 0.0005 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.3551 | 7.0424 | 7.8386 | 12.231 | 9.8949 | 19.1919 | 13.2398 | 16.2199 | 13.7975 | 12.277 |
Translational Efficiency | 1.0211 | 1.206 | 1.092 | 0.7653 | 0.6297 | 0.6783 | 0.9497 | 0.6597 | 0.961 | 0.763 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1258 | 1247 | 227 | 1326 | 1547 | 1564 | 1539 | 437 | 2805 | 2811 | 1766 | 1763 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 745.90 | 850.21 | 1243.08 | 1085.62 | 791.04 | 858.91 | 1022.70 | 1529.19 | 770.80 | 855.05 | 1051.03 | 1195.57 |
Standard Deviation | 84.64 | 129.35 | 157.39 | 168.38 | 102.76 | 134.06 | 124.15 | 405.51 | 97.68 | 132.06 | 148.51 | 314.27 |
Intensity Change Log 2 | — | 0.188837 | 0.736865 | 0.541465 | — | 0.118756 | 0.370560 | 0.950945 | — | 0.153193 | 0.559947 | 0.766645 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.002014 | 0.004786 | 0.056717 | 0.034877 | 0.002605 | 0.003248 | 0.007697 | 0.008299 | 0.002340 | 0.003930 | 0.013998 | 0.028289 |
Bud Neck | 0.018579 | 0.015638 | 0.013273 | 0.010385 | 0.018379 | 0.023328 | 0.009426 | 0.022098 | 0.018469 | 0.019917 | 0.009920 | 0.013288 |
Bud Site | 0.018462 | 0.053481 | 0.113845* | 0.118604* | 0.026735 | 0.048925 | 0.035004 | 0.049887 | 0.023025 | 0.050947 | 0.045138 | 0.101571* |
Cell Periphery | 0.000296 | 0.000144 | 0.000716 | 0.000190 | 0.000213 | 0.000167 | 0.000200 | 0.000052 | 0.000250 | 0.000157 | 0.000266 | 0.000156 |
Cytoplasm | 0.372911* | 0.377377* | 0.312244* | 0.251676* | 0.404125* | 0.313342* | 0.502865* | 0.373199* | 0.390126* | 0.341749* | 0.478363* | 0.281799* |
Cytoplasmic Foci | 0.381424* | 0.322505* | 0.097737 | 0.208033* | 0.339527* | 0.320853* | 0.112749 | 0.181445* | 0.358317* | 0.321586* | 0.110819 | 0.201443* |
Eisosomes | 0.000314 | 0.000142 | 0.000162 | 0.000107 | 0.000282 | 0.000188 | 0.000086 | 0.000046 | 0.000296 | 0.000167 | 0.000096 | 0.000092 |
Endoplasmic Reticulum | 0.002961 | 0.002124 | 0.009749 | 0.001148 | 0.004039 | 0.001921 | 0.002521 | 0.000126 | 0.003556 | 0.002011 | 0.003450 | 0.000895 |
Endosome | 0.022347 | 0.042616 | 0.015642 | 0.037750 | 0.032357 | 0.067378 | 0.017069 | 0.039526 | 0.027868 | 0.056393 | 0.016886 | 0.038190 |
Golgi | 0.022102 | 0.020056 | 0.002588 | 0.010695 | 0.017355 | 0.027538 | 0.002476 | 0.020055 | 0.019484 | 0.024219 | 0.002491 | 0.013015 |
Lipid Particles | 0.015730 | 0.004750 | 0.012545 | 0.006433 | 0.009649 | 0.007152 | 0.003885 | 0.003710 | 0.012377 | 0.006086 | 0.004998 | 0.005758 |
Mitochondria | 0.014967 | 0.005313 | 0.000355 | 0.001973 | 0.006872 | 0.010685 | 0.000495 | 0.007066 | 0.010503 | 0.008302 | 0.000477 | 0.003235 |
Mitotic Spindle | 0.003333 | 0.013177 | 0.054549 | 0.077701 | 0.003376 | 0.009859 | 0.020495 | 0.076588 | 0.003357 | 0.011331 | 0.024873 | 0.077425 |
None | 0.003592 | 0.001175 | 0.000861 | 0.000253 | 0.001908 | 0.001258 | 0.001954 | 0.000447 | 0.002663 | 0.001221 | 0.001814 | 0.000301 |
Nuclear Periphery | 0.000274 | 0.000186 | 0.000821 | 0.000337 | 0.000224 | 0.000194 | 0.000762 | 0.000220 | 0.000246 | 0.000191 | 0.000770 | 0.000308 |
Nuclear Periphery Foci | 0.000885 | 0.001831 | 0.013555 | 0.002015 | 0.001110 | 0.001193 | 0.005230 | 0.000236 | 0.001009 | 0.001476 | 0.006300 | 0.001574 |
Nucleolus | 0.001541 | 0.001346 | 0.000210 | 0.000296 | 0.000558 | 0.001560 | 0.000865 | 0.000725 | 0.000999 | 0.001465 | 0.000781 | 0.000403 |
Nucleus | 0.049892 | 0.018565 | 0.109256 | 0.007632 | 0.033803 | 0.018646 | 0.082632 | 0.010975 | 0.041019 | 0.018610 | 0.086054 | 0.008461 |
Peroxisomes | 0.030672 | 0.033520 | 0.016532 | 0.117250* | 0.023480 | 0.035924 | 0.030817 | 0.101608* | 0.026706 | 0.034857 | 0.028981 | 0.113373* |
Vacuole | 0.034282 | 0.077452 | 0.168276 | 0.108790 | 0.069795 | 0.101203 | 0.162313 | 0.101996 | 0.053868 | 0.090667 | 0.163080 | 0.107106 |
Vacuole Periphery | 0.003420 | 0.003816 | 0.000366 | 0.003853 | 0.003607 | 0.005436 | 0.000460 | 0.001697 | 0.003523 | 0.004718 | 0.000448 | 0.003319 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.09 | -4.88 | -9.42 | -8.13 | 1.50 | -1.01 | -3.67 | -1.96 | -1.66 | -0.39 | -2.99 | -6.16 | -9.54 | -8.63 | -4.85 |
Bud Neck | 1.64 | 1.05 | 5.59 | 4.35 | 3.63 | -2.55 | 6.00 | -1.57 | 0.40 | -6.01 | -0.93 | 6.86 | 4.80 | 6.17 | -2.62 |
Bud Site | -10.76 | -7.31 | -17.72 | -9.02 | -0.58 | -7.41 | -1.33 | -3.23 | 1.61 | -2.34 | -12.66 | -5.93 | -17.23 | -8.68 | -11.34 |
Cell Periphery | 3.46 | -6.39 | 2.67 | -2.46 | 10.30 | 2.50 | 1.89 | 11.57 | 9.47 | 8.94 | 4.21 | 0.11 | 4.68 | 0.95 | 6.14 |
Cytoplasm | -0.46 | 3.96 | 7.13 | 7.22 | 0.55 | 8.95 | -5.68 | 0.68 | -4.66 | 4.15 | 6.26 | -6.01 | 8.11 | 2.61 | 12.43 |
Cytoplasmic Foci | 6.51 | 29.62 | 24.51 | 17.53 | -10.55 | 2.28 | 34.04 | 16.52 | 14.69 | -6.12 | 6.08 | 46.04 | 29.90 | 23.91 | -13.08 |
Eisosomes | 5.76 | 6.55 | 10.95 | 2.04 | 2.51 | 2.26 | 5.08 | 6.16 | 8.56 | 6.33 | 4.88 | 8.73 | 9.30 | 6.04 | 1.93 |
Endoplasmic Reticulum | 1.08 | -5.18 | 3.31 | 1.70 | 7.54 | 3.89 | 2.82 | 7.95 | 7.55 | 15.04 | 3.33 | 0.31 | 7.07 | 3.70 | 11.08 |
Endosome | -7.25 | 4.22 | -2.53 | 4.98 | -6.14 | -10.78 | 9.57 | -0.25 | 7.64 | -5.57 | -12.94 | 10.01 | -0.66 | 11.22 | -8.95 |
Golgi | 1.25 | 13.29 | 9.42 | 7.93 | -7.44 | -4.83 | 12.49 | 1.05 | 4.12 | -4.77 | -2.90 | 17.44 | 8.78 | 10.84 | -7.82 |
Lipid Particles | 9.32 | 1.95 | 7.68 | -2.78 | 4.28 | 2.65 | 7.49 | 6.83 | 4.58 | 0.25 | 8.51 | 10.70 | 8.69 | 0.24 | -1.98 |
Mitochondria | 5.29 | 8.45 | 7.78 | 5.37 | -3.08 | -2.48 | 6.71 | 0.72 | 2.76 | -3.86 | 1.98 | 10.69 | 7.92 | 7.10 | -4.75 |
Mitotic Spindle | -4.65 | -3.72 | -13.20 | -10.50 | -3.32 | -3.75 | -7.30 | -7.50 | -6.64 | -5.06 | -5.94 | -8.27 | -15.25 | -12.93 | -9.10 |
None | 3.25 | 3.78 | 4.56 | 3.27 | 4.68 | 1.10 | 0.20 | 3.06 | 1.72 | 5.78 | 3.11 | 2.26 | 5.65 | 3.27 | 7.15 |
Nuclear Periphery | 1.47 | -8.72 | -2.08 | -3.73 | 7.41 | 0.53 | -14.34 | -2.33 | -2.67 | 6.58 | 1.44 | -16.29 | -3.40 | -4.72 | 10.98 |
Nuclear Periphery Foci | -2.60 | -11.17 | -4.19 | -1.40 | 9.64 | -0.45 | -7.51 | 6.43 | 3.80 | 8.95 | -2.25 | -10.59 | -3.45 | -1.48 | 8.03 |
Nucleolus | 0.41 | 4.06 | 3.84 | 2.31 | -1.01 | -2.19 | -3.06 | -3.56 | 1.07 | -0.92 | -1.37 | 0.94 | 3.20 | 2.93 | 2.96 |
Nucleus | 12.35 | -7.80 | 15.20 | 3.30 | 11.23 | 8.12 | -17.19 | 7.44 | 1.09 | 21.31 | 14.55 | -17.32 | 17.44 | 3.81 | 26.35 |
Peroxisomes | -0.73 | 5.79 | -10.10 | -9.80 | -14.25 | -3.99 | 0.55 | -7.30 | -5.72 | -7.49 | -3.46 | 2.53 | -13.28 | -11.36 | -14.37 |
Vacuole | -12.04 | -14.12 | -23.26 | -14.41 | 3.22 | -7.56 | -23.13 | -7.97 | -4.47 | 6.22 | -13.12 | -29.42 | -22.06 | -14.05 | 5.96 |
Vacuole Periphery | -0.55 | 7.52 | 1.42 | 1.92 | -3.56 | -2.47 | 7.45 | 4.61 | 6.60 | -4.59 | -2.40 | 10.47 | 2.57 | 4.41 | -4.15 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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