Standard name
Human Ortholog
Description Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.11 – 0.05 0 0 0 – 0 0.05 0 0 0.13 0.2 0.21 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0.16 0 0 0 – 0 0.07 0.07 0.11 0 0.05 0.05 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.09 0.2 – 0.11 0.1 0 0.1 – 0.13 0.23 0.18 0.14 0.37 0.53 0.59 0 0 0 0 0.08 0.1 0 0 0
Cytoplasm 0.25 0.32 – 0.79 0.47 0.72 0.79 – 0.87 0.89 0.95 0.88 0.45 0.35 0.27 0.15 0.25 0.19 0 0.05 0 0 0 0
Endoplasmic Reticulum 0.5 0.45 – 0.05 0.28 0.09 0.11 – 0.08 0 0 0 0.25 0.18 0.16 0 0 0.06 0.28 0.29 0.25 0.18 0.15 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.09 0 – 0 0.07 0.07 0 – 0 0 0 0 0 0 0 0.23 0.17 0.18 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0 – 0 0.26 0.13 0.09 – 0 0.07 0 0.06 0 0 0 0.62 0.58 0.64 0.58 0.45 0.35 0.7 0.69 0.83
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 28 – 1 0 3 7 – 3 10 2 5 25 68 77 2 2 1 3 10 1 3 13 1
Bud 1 3 – 3 1 2 1 – 4 12 7 13 4 18 18 3 0 0 0 1 0 0 2 3
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 4 23 1 4 5 2
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 18 50 – 2 7 1 21 – 17 41 18 17 69 184 219 2 1 2 9 27 2 0 7 0
Cytoplasm 50 79 – 15 34 55 161 – 109 162 93 107 84 120 102 15 40 26 3 15 1 5 6 8
Endoplasmic Reticulum 103 113 – 1 20 7 22 – 10 3 2 2 47 62 61 2 6 8 74 95 7 43 58 19
Endosome 1 0 – 0 0 0 0 – 0 0 0 0 2 2 1 0 5 2 1 3 1 4 18 21
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 3 8 6 3 0 0 0 2 3
Mitochondria 5 3 – 0 0 0 0 – 2 0 0 0 0 0 1 3 3 3 9 2 0 4 1 5
Nucleus 19 8 – 0 5 5 9 – 2 2 1 5 1 0 0 23 28 25 0 0 0 0 0 2
Nuclear Periphery 0 0 – 1 0 0 0 – 1 0 0 0 0 1 0 0 0 1 2 1 1 1 3 4
Nucleolus 0 0 – 0 0 1 1 – 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 1 – 0 1 0 0 1 4 1 0 1 1 0 0 0 0 0 0
Vac/Vac Membrane 27 6 – 0 19 10 19 – 2 12 1 7 2 4 1 61 94 89 153 151 9 168 276 368
Unique Cell Count 204 250 19 72 76 203 126 182 98 121 186 346 371 99 161 139 267 335 28 239 399 445
Labelled Cell Count 232 290 23 86 84 242 150 243 124 156 235 463 482 114 188 166 267 335 28 239 399 445


Cytoplasm, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.6 10.3 – 7.6 12.0 7.8 10.5 – 8.6 9.2 8.1 9.0 9.4 9.9 10.2 15.2 16.2 17.4 8.8 8.8 9.4
Std Deviation (1e-4) 1.4 1.5 – 1.2 1.8 1.6 1.8 – 1.5 1.6 1.4 1.8 1.3 2.0 1.6 4.6 4.9 4.8 1.7 2.0 2.3
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4445 3.435 2.9387 3.0019 2.5293 3.0943 4.922 6.8387 6.1237 5.8693 5.7976 6.511 6.104 7.0021 6.1659 6.1434 5.8969 6.815
Actin 0.0141 0.0001 0.0021 0.0003 0.0009 0.0004 0.024 0.0001 0.0032 0.0142 0.0004 0.0051 0.0163 0.0001 0.0011 0.0172 0 0.0001
Bud 0.0008 0.0036 0.0013 0.0022 0.001 0.0006 0.0008 0.0004 0.0009 0.0012 0.0009 0.0004 0.0004 0.0008 0.001 0.0005 0.0005 0.0004
Bud Neck 0.004 0.0019 0.0016 0.0008 0.0009 0.0037 0.0131 0.0061 0.0035 0.0024 0.0037 0.0094 0.0027 0.0024 0.0025 0.002 0.0013 0.0046
Bud Periphery 0.0024 0.0031 0.0021 0.0135 0.0024 0.0027 0.0028 0.0016 0.0035 0.0043 0.0057 0.003 0.0016 0.0027 0.0027 0.0013 0.0029 0.0021
Bud Site 0.004 0.0128 0.0042 0.0013 0.0024 0.0014 0.0096 0.0143 0.0099 0.0121 0.0038 0.0011 0.0035 0.0089 0.0089 0.0031 0.0013 0.0009
Cell Periphery 0.0211 0.0233 0.0168 0.0086 0.0094 0.018 0.109 0.0802 0.0693 0.0584 0.0852 0.1015 0.0361 0.0216 0.0152 0.0148 0.0119 0.0226
Cytoplasm 0.0206 0.0299 0.0155 0.0045 0.013 0.0124 0.0486 0.0573 0.0678 0.0355 0.0134 0.0219 0.0368 0.0261 0.017 0.0027 0.0041 0.0263
Cytoplasmic Foci 0.0105 0.0051 0.0101 0.0029 0.0099 0.0083 0.0343 0.006 0.0092 0.0079 0.0119 0.0046 0.0112 0.0051 0.0086 0.0049 0.0009 0.0034
Eisosomes 0.0003 0 0 0 0 0 0.0002 0 0 0.0001 0 0 0.0003 0 0 0.0002 0 0
Endoplasmic Reticulum 0.0237 0.0229 0.0131 0.0044 0.0041 0.0107 0.0196 0.0324 0.034 0.0183 0.008 0.0144 0.0354 0.0239 0.0204 0.0043 0.0068 0.0178
Endosome 0.1328 0.1061 0.1111 0.0908 0.1144 0.0812 0.0859 0.0407 0.0726 0.0591 0.0983 0.0302 0.1052 0.0845 0.1349 0.0725 0.0604 0.0658
Golgi 0.0085 0.0034 0.0041 0.0029 0.0072 0.0046 0.0138 0.0014 0.0058 0.0173 0.0151 0.0076 0.01 0.0033 0.0059 0.0059 0.0008 0.0019
Lipid Particles 0.0265 0.004 0.0086 0.0016 0.014 0.0074 0.0797 0.0323 0.028 0.0292 0.0094 0.0175 0.0367 0.0082 0.0121 0.0172 0.0026 0.0089
Mitochondria 0.0131 0.0011 0.0046 0.0088 0.0051 0.0064 0.0155 0.0002 0.0019 0.0025 0.0034 0.0035 0.0042 0.0036 0.0058 0.0038 0.0005 0.0009
None 0.0086 0.0005 0.0116 0.001 0.0006 0.0031 0.0054 0.0005 0.0009 0.0003 0.0001 0.0001 0.0134 0.0003 0.0007 0.0004 0.0001 0.007
Nuclear Periphery 0.0068 0.0066 0.0063 0.0027 0.0032 0.0044 0.0064 0.0038 0.0055 0.0024 0.0018 0.002 0.0116 0.0066 0.0053 0.0026 0.0061 0.0031
Nucleolus 0.0016 0.0014 0.0021 0.0013 0.0008 0.0013 0.0012 0.0017 0.0017 0.0006 0.001 0.0009 0.0027 0.0029 0.002 0.0016 0.0012 0.001
Nucleus 0.0032 0.004 0.0069 0.0027 0.0021 0.0036 0.0032 0.003 0.0031 0.0017 0.0018 0.0019 0.0118 0.0043 0.0031 0.002 0.0048 0.0035
Peroxisomes 0.0056 0.0001 0.0033 0.0003 0.0019 0.0004 0.0055 0.0001 0.0009 0.0016 0.0012 0.0007 0.0029 0.0002 0.0019 0.0019 0.0001 0.0001
Punctate Nuclear 0.0031 0.0001 0.001 0.0001 0.001 0.0003 0.0111 0.0002 0.0003 0.0003 0.0002 0.0002 0.0018 0.0001 0.0003 0.0009 0.0001 0.0001
Vacuole 0.6456 0.7306 0.7121 0.78 0.7697 0.7848 0.5028 0.7119 0.6667 0.7204 0.7282 0.7675 0.6317 0.7545 0.701 0.7895 0.8374 0.7954
Vacuole Periphery 0.0433 0.0395 0.0615 0.0693 0.0358 0.0445 0.0074 0.0059 0.0114 0.0103 0.0064 0.0062 0.0236 0.04 0.0497 0.0506 0.0564 0.0341

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.4313 20.2573 18.5007 24.0389 21.4705 36.5856 22.0577 29.2432 31.6396 29.0996
Translational Efficiency 0.8016 0.9167 0.7899 0.8561 0.6953 0.5031 1.0395 1.1834 1.0009 0.6295

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
249 1235 206 1707 1684 2286 323 1873 1933 3521 529 3580

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 917.44 1040.55 1215.71 932.61 797.41 986.40 1207.90 1049.20 812.87 1005.39 1210.94 993.61
Standard Deviation 185.35 344.03 130.02 130.32 110.89 123.73 141.35 135.42 129.44 228.30 137.10 145.20
Intensity Change Log 2 — 0.181661 0.406113 0.023660 — 0.306851 0.599107 0.395896 — 0.241228 0.499075 0.208736

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000069 0.001772 0.041640 0.004633 0.000145 0.001354 0.016711 0.005591 0.000135 0.001500 0.026419 0.005134
Bud Neck 0.002440 0.058279 0.076470 0.016571 0.028346 0.073574 0.066778 0.044906 0.025009 0.068209 0.070552 0.031396
Bud Site 0.016020 0.042656 0.125769* 0.076055 0.018616 0.026365 0.076238 0.096618 0.018282 0.032079 0.095526 0.086813
Cell Periphery 0.007842 0.145525* 0.295604* 0.001297 0.009644 0.251330* 0.197678* 0.015427 0.009412 0.214219* 0.235812* 0.008690
Cytoplasm 0.016720 0.132795* 0.105615* 0.077921 0.060462 0.083881 0.142850* 0.105758* 0.054827 0.101038* 0.128351* 0.092485
Cytoplasmic Foci 0.006488 0.007815 0.008995 0.063928 0.015240 0.004944 0.011071 0.030935 0.014112 0.005951 0.010262 0.046667
Eisosomes 0.000014 0.000975 0.010672 0.000089 0.000109 0.001092 0.004782 0.000668 0.000097 0.001051 0.007075 0.000392
Endoplasmic Reticulum 0.002790 0.031674 0.095684 0.005587 0.009471 0.036707 0.070116 0.016532 0.008610 0.034941 0.080073 0.011313
Endosome 0.019007 0.017279 0.009751 0.204907* 0.037311 0.012164 0.014253 0.107187 0.034953 0.013958 0.012500 0.153782
Golgi 0.002245 0.002300 0.005186 0.020700 0.001553 0.002946 0.003101 0.009585 0.001642 0.002720 0.003913 0.014885
Lipid Particles 0.001886 0.003429 0.043522 0.002722 0.001516 0.004755 0.024314 0.009950 0.001563 0.004290 0.031794 0.006504
Mitochondria 0.000818 0.000340 0.000124 0.002121 0.000673 0.002851 0.000040 0.001708 0.000691 0.001970 0.000073 0.001905
Mitotic Spindle 0.000347 0.000050 0.000193 0.001297 0.000024 0.000111 0.000150 0.002866 0.000065 0.000089 0.000167 0.002118
None 0.000110 0.000421 0.000015 0.000094 0.000067 0.000037 0.000004 0.000236 0.000072 0.000171 0.000008 0.000168
Nuclear Periphery 0.000138 0.000069 0.000207 0.000084 0.000065 0.000091 0.000151 0.000102 0.000075 0.000083 0.000173 0.000093
Nuclear Periphery Foci 0.001863 0.000648 0.006031 0.000613 0.000148 0.000552 0.005766 0.000869 0.000369 0.000586 0.005869 0.000747
Nucleolus 0.000371 0.000253 0.000023 0.000214 0.000214 0.000286 0.000057 0.000317 0.000234 0.000274 0.000044 0.000268
Nucleus 0.005938 0.003554 0.003558 0.001322 0.002572 0.001419 0.002254 0.002189 0.003006 0.002168 0.002762 0.001776
Peroxisomes 0.000375 0.001045 0.001632 0.005109 0.000654 0.001786 0.001630 0.003885 0.000618 0.001526 0.001631 0.004469
Vacuole 0.864643* 0.545178* 0.168798 0.486885* 0.803105* 0.489966* 0.361175* 0.528936* 0.811032* 0.509332* 0.286261* 0.508885*
Vacuole Periphery 0.049878 0.003945 0.000513 0.027850 0.010065 0.003790 0.000881 0.015734 0.015193 0.003844 0.000737 0.021511

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.52 -7.11 -5.19 -3.11 6.27 -3.78 -9.15 -9.11 -6.22 5.87 -6.00 -10.17 -9.54 -6.34 8.10
Bud Neck -16.62 -8.68 -13.07 11.87 6.95 -13.88 -6.53 -6.27 8.49 3.73 -16.55 -9.10 -3.34 14.98 7.98
Bud Site -5.43 -8.41 -11.92 -7.39 3.84 -3.35 -7.99 -19.40 -16.63 -2.49 -6.65 -11.52 -24.15 -18.15 1.25
Cell Periphery -23.65* -15.58 4.63 25.49* 15.99 -46.13* -16.10 -3.25 44.08* 15.56 -50.38 -21.93 0.79 50.66* 22.02
Cytoplasm -15.44 -8.88 -10.74 7.57 2.67 -5.05 -9.48 -9.48 -4.75 3.91 -11.10 -10.94 -10.11 1.65 5.09
Cytoplasmic Foci -0.58 -0.96 -17.06 -20.73 -17.13 7.41 1.70 -7.96 -15.52 -8.08 6.56 1.98 -17.49 -25.95 -17.85
Eisosomes -6.15 -8.81 -6.30 5.66 8.75 -12.14 -9.10 -5.69 3.50 7.90 -12.51 -12.06 -5.59 7.33 11.52
Endoplasmic Reticulum -12.24 -8.16 -3.86 11.01 7.91 -13.40 -8.55 -4.52 9.19 7.52 -16.13 -11.46 -2.50 14.79 11.05
Endosome 0.39 2.02 -27.15* -34.09* -35.22* 11.82 9.08 -17.17 -25.79 -23.59* 10.83 10.50 -32.05 -41.90* -40.85*
Golgi -0.05 -2.58 -10.37 -12.22 -9.81 -2.24 -3.12 -7.69 -5.81 -6.02 -2.28 -5.13 -14.24 -12.71 -11.65
Lipid Particles -1.00 -6.80 -0.58 1.70 6.85 -6.76 -7.00 -9.47 -5.26 4.26 -6.99 -9.81 -9.32 -3.94 8.13
Mitochondria 1.12 1.62 -2.71 -7.97 -8.89 -3.43 3.16 -3.36 1.80 -7.24 -2.95 3.36 -4.98 0.20 -11.44
Mitotic Spindle 0.99 0.52 -2.05 -3.24 -2.90 -2.48 -1.44 -4.36 -4.24 -4.12 -0.52 -1.53 -5.27 -5.23 -4.97
None -1.59 1.43 0.17 1.70 -1.52 0.88 1.86 -1.23 -1.51 -1.76 -1.41 2.11 -1.21 0.10 -2.20
Nuclear Periphery 0.95 -0.89 0.67 -1.58 5.52 -2.07 -5.58 -2.84 -1.73 2.81 -0.59 -5.81 -1.58 -2.01 5.98
Nuclear Periphery Foci 0.81 -2.61 0.78 -0.28 10.45 -4.50 -12.87 -6.26 -2.44 10.69 -1.05 -14.40 -2.03 -2.02 14.84
Nucleolus 0.65 2.06 0.85 0.32 -13.65 -2.35 11.30 -4.11 -1.12 -10.26 -1.01 7.78 -1.65 -0.19 -15.00
Nucleus 0.52 0.69 1.39 2.98 3.77 2.86 0.66 0.33 -3.30 -0.39 0.93 0.30 1.86 1.24 2.74
Peroxisomes -3.36 -4.72 -10.67 -9.55 -7.55 -5.81 -5.22 -6.96 -4.27 -4.62 -6.42 -6.77 -12.12 -8.83 -8.43
Vacuole 18.51 36.78* 22.68 4.79 -22.78 37.22* 30.05 29.39 -4.00 -10.87 40.50* 45.11* 39.73 0.05 -19.27
Vacuole Periphery 6.86 7.38 3.29 -19.43 -23.18* 8.93 13.98 -4.77 -11.31 -14.53 10.45 13.57 -4.67 -21.67 -26.44*
Standard name
Human Ortholog
Description Vacuolar protein; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (74%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rcr2

Rcr2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rcr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available