Standard name
Human Ortholog
Description Subunit of a complex that inhibits sister chromatid cohesion; also negatively regulates chromosome condensation; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; binds Smc3p ATPase head of cohesin; related to the human Wapl protein that controls the association of cohesin with chromatin
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.33 0.26 0.46 0.46 0.52 0.3 0.36 0.37 0.28 0.27 0.17 0.26 0.44 0.51 0.52 0.38 0.44 0.48 0.17 0.13 0.15 0.35 0.38 0.51
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 0 0.06 0.06 0.06 0 0 0 0 0 0 0.06 0 0 0.07 0.13 0.08 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.38 0.05 0.05 0.05 0.06 0.36 0.25 0.27 0.5 0.37 0.74 0.53 0 0 0 0.11 0.05 0.08 0 0 0.09 0 0 0
Nucleus 0.32 0.56 0.37 0.37 0.17 0.3 0.32 0.33 0.23 0.34 0.25 0.24 0.42 0.43 0.39 0.28 0.24 0.24 0.38 0.5 0.24 0.27 0.2 0.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0.06 0.09 0 0.06 0.06 0.05 0.05 0 0 0 0 0 0 0 0.05 0.11 0.1 0.17 0 0 0
Vac/Vac Membrane 0.1 0.24 0.11 0.11 0.19 0.14 0.12 0.08 0.09 0.17 0.06 0.17 0.14 0 0.08 0.24 0.19 0.16 0.19 0.13 0.18 0.27 0.28 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 1 1 0 1 1 1 3 2 2 1 0 0 0 1 0 0 3 16 5 2 4 6
Bud Neck 1 1 1 1 0 2 0 1 3 2 0 0 0 2 1 0 0 1 2 1 0 3 3 5
Bud Site 0 0 0 1 0 3 1 3 4 3 5 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 47 127 72 72 88 48 67 88 68 68 28 45 58 91 119 136 143 144 32 76 33 87 108 145
Endoplasmic Reticulum 0 2 0 0 0 1 2 0 2 0 2 0 2 1 1 11 19 16 0 0 1 1 0 2
Endosome 0 3 7 9 10 10 6 6 10 6 0 2 8 6 7 26 41 23 5 15 5 1 5 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 1 0 0 1
Mitochondria 55 25 8 8 10 57 46 64 120 93 120 92 1 1 2 40 17 25 4 11 18 4 5 12
Nucleus 46 270 59 59 29 48 59 79 56 87 41 41 56 76 89 100 77 73 74 302 53 67 55 28
Nuclear Periphery 0 0 0 0 0 0 2 1 0 0 2 2 0 0 0 1 1 1 0 0 2 0 0 0
Nucleolus 2 2 1 1 2 2 1 3 3 1 0 0 1 0 1 8 4 2 6 29 11 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0
SpindlePole 3 9 8 9 15 7 12 15 11 12 2 3 2 5 8 10 10 14 21 59 36 7 10 9
Vac/Vac Membrane 14 115 17 17 32 22 22 18 22 43 10 29 18 8 18 84 61 48 36 80 40 66 78 60
Unique Cell Count 143 482 158 158 169 160 186 239 240 254 163 172 133 178 227 355 325 299 195 606 220 250 283 283
Labelled Cell Count 168 554 174 178 186 201 219 279 303 318 212 215 146 190 248 419 375 348 195 606 220 250 283 283


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.6 5.2 5.2 5.7 4.4 4.9 4.9 4.3 4.5 3.8 4.1 5.6 5.7 5.6 6.1 6.0 5.7 5.0 5.2 5.7
Std Deviation (1e-4) 0.8 0.7 1.8 1.8 1.8 1.5 1.9 1.4 1.7 1.3 1.3 1.1 1.1 1.2 1.4 1.6 1.6 1.7 1.0 1.5 1.5
Intensity Change (Log2) – – – 0.0 0.13 -0.24 -0.1 -0.08 -0.28 -0.21 -0.47 -0.34 0.1 0.13 0.1 0.21 0.2 0.12 -0.07 -0.0 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.4 2.2 1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.4984 0.0642 -0.1696 -0.422 0.3172 -0.355 0.0437 -0.1071 0.2043 0.1955 -0.3762 0.1308 0.8517 0.284 0.0999 1.022 0.5277 0.0475
Actin 0.0454 0.0003 0.0111 0.0003 0.0091 0.0015 0.0219 0.0012 0.0097 0.0024 0.001 0.0088 0.0294 0.0004 0.0128 0.0709 0.0509 0.0027
Bud 0.0012 0.0011 0.0007 0 0.0057 0.0027 0.0008 0.0001 0.0002 0.0002 0.0001 0.0003 0.0011 0.0002 0.0017 0.0007 0.0008 0.0033
Bud Neck 0.0075 0.0009 0.0026 0.0002 0.0013 0.0044 0.0161 0.0011 0.0036 0.0256 0.0004 0.0041 0.007 0.0008 0.0024 0.01 0.0016 0.0038
Bud Periphery 0.0028 0.0007 0.0011 0 0.0098 0.0059 0.0016 0.0002 0.0003 0.0004 0.0001 0.0006 0.0024 0.0001 0.0007 0.0013 0.0016 0.0008
Bud Site 0.0128 0.0083 0.0019 0.0001 0.0199 0.0015 0.0139 0.004 0.004 0.002 0.0003 0.0006 0.0055 0.007 0.0045 0.0029 0.0016 0.0008
Cell Periphery 0.0008 0.0001 0.0002 0 0.0006 0.0001 0.0007 0.0002 0.0002 0.0003 0 0.0001 0.0006 0 0.0002 0.0006 0.0002 0.0001
Cytoplasm 0.0791 0.1142 0.0435 0.0213 0.0345 0.0595 0.0708 0.0596 0.035 0.0331 0.0521 0.0652 0.0574 0.0768 0.0189 0.0232 0.0208 0.0588
Cytoplasmic Foci 0.0376 0.0041 0.0067 0.0116 0.0122 0.0105 0.0378 0.0015 0.0142 0.0051 0.0292 0.0112 0.0171 0.0035 0.004 0.0119 0.0141 0.0102
Eisosomes 0.0005 0 0.0001 0 0.0003 0 0.0005 0.0001 0.0002 0.0001 0 0.0001 0.0005 0 0.0001 0.0005 0.0005 0
Endoplasmic Reticulum 0.0079 0.0026 0.0041 0.0007 0.0028 0.0016 0.0044 0.0016 0.0012 0.0011 0.0016 0.0015 0.0079 0.0028 0.0011 0.0055 0.0047 0.0016
Endosome 0.0437 0.0032 0.0154 0.0062 0.0338 0.0093 0.0255 0.0014 0.0139 0.0037 0.0476 0.0076 0.0312 0.0024 0.004 0.021 0.0375 0.0059
Golgi 0.0157 0.0001 0.0069 0.0011 0.0085 0.0058 0.0067 0.0003 0.0069 0.0016 0.0184 0.0049 0.007 0.0001 0.0016 0.0139 0.0254 0.0009
Lipid Particles 0.0412 0.0005 0.0029 0.0578 0.0186 0.0099 0.0306 0.0002 0.0064 0.0137 0.0328 0.0125 0.0174 0.0002 0.0013 0.0287 0.0274 0.0019
Mitochondria 0.0226 0.0007 0.0109 0.0006 0.054 0.0109 0.0205 0.0022 0.0148 0.0011 0.0048 0.0111 0.0179 0.0007 0.0017 0.009 0.0812 0.0009
None 0.244 0.1518 0.0868 0.0704 0.1102 0.0619 0.2184 0.1379 0.0931 0.0981 0.1569 0.1552 0.0917 0.0685 0.0381 0.0136 0.0579 0.0923
Nuclear Periphery 0.0672 0.0198 0.0302 0.0091 0.0315 0.0161 0.0318 0.0158 0.0212 0.007 0.041 0.0112 0.0552 0.0211 0.014 0.012 0.0607 0.0199
Nucleolus 0.0095 0.0111 0.0159 0.0065 0.009 0.0194 0.0094 0.0116 0.0252 0.0137 0.0052 0.0109 0.0133 0.0088 0.012 0.0058 0.0068 0.0131
Nucleus 0.2767 0.6051 0.643 0.6754 0.5224 0.6968 0.4217 0.7139 0.6323 0.7287 0.5201 0.6224 0.5519 0.7391 0.7596 0.5939 0.4741 0.695
Peroxisomes 0.022 0.0002 0.0028 0.0766 0.0064 0.003 0.0267 0.0002 0.011 0.0354 0.006 0.0091 0.0095 0.0002 0.0009 0.0125 0.01 0.0009
Punctate Nuclear 0.0466 0.0723 0.11 0.0613 0.0637 0.0772 0.0334 0.0453 0.1029 0.0244 0.0793 0.0595 0.0544 0.065 0.1184 0.1596 0.1169 0.085
Vacuole 0.0076 0.0024 0.0019 0.0006 0.0337 0.0016 0.0051 0.0015 0.0029 0.0019 0.0016 0.0022 0.015 0.002 0.0013 0.0014 0.0026 0.0015
Vacuole Periphery 0.0074 0.0005 0.0013 0.0002 0.0118 0.0005 0.0016 0.0003 0.0007 0.0003 0.0014 0.0009 0.0066 0.0004 0.0007 0.0009 0.0027 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.7977 3.5518 2.2591 6.6291 6.2552 6.8074 6.4128 5.8864 5.1512 8.6606
Translational Efficiency 0.934 0.963 1.6205 0.7134 0.7314 1.2561 0.6244 0.6724 0.9684 0.7705

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of a complex that inhibits sister chromatid cohesion; also negatively regulates chromosome condensation; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; binds Smc3p ATPase head of cohesin; related to the human Wapl protein that controls the association of cohesin with chromatin
Localization
Cell Percentages nucleus (73%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rad61

Rad61


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad61-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available