Standard name
Human Ortholog
Description Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0.05 0.07 0.09 0.06 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0.09 0.12 0.14 0.11 0.09 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.09 0 – 0 0 0 0 0 0.11 0.08 0.1 0.09 0.07 0.05 0.06 0 0.05 0.07 0.06 0.07
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.05 0.09 0.08 0 0.08 – 0.06 0.09 0.07 0.08 0.08 0 0 0 0.08 0.08 0 0 0 0 0 0.06 0.06
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.76 0.83 0.48 0.61 0.5 0.53 – 0.5 0.57 0.51 0.54 0.55 0.67 0.57 0.58 0.68 0.8 0.74 0.12 0.13 0.06 0.07 0.1 0.11
Peroxisomes 0 0.05 0.12 0.25 0.26 0.29 – 0.18 0.22 0.15 0.18 0.18 0.13 0.13 0.14 0.09 0.07 0.06 0 0 0 0 0 0
SpindlePole 0.54 0.56 0.74 0.56 0.61 0.6 – 0.56 0.56 0.65 0.54 0.54 0.55 0.52 0.52 0.38 0.33 0.39 0.62 0.64 0.75 0.58 0.51 0.47
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1
Bud 0 0 15 4 5 5 – 24 9 9 16 13 0 0 0 0 0 1 5 2 3 1 5 3
Bud Neck 5 4 9 2 6 10 – 13 8 8 20 8 4 4 3 0 1 1 8 4 2 1 2 4
Bud Site 7 0 18 11 5 10 – 31 26 34 27 33 1 2 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 5 3 5 10 2 – 8 2 4 7 6 26 39 51 15 12 22 0 0 0 0 1 1
Endoplasmic Reticulum 1 0 0 0 0 2 – 0 0 0 1 0 0 0 1 2 0 1 0 0 0 0 0 0
Endosome 9 10 7 5 35 15 – 6 3 0 4 8 33 25 36 13 9 11 36 11 22 15 16 23
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 3 2 1 0 2 0
Mitochondria 37 13 45 29 7 32 – 37 34 26 34 34 4 4 2 11 11 7 17 5 8 7 15 22
Nucleus 20 6 17 11 9 14 – 16 17 12 9 11 6 11 3 6 8 13 11 3 5 4 2 2
Nuclear Periphery 4 1 2 0 1 0 – 4 2 1 2 1 1 1 0 1 1 0 2 0 0 1 1 1
Nucleolus 382 220 228 212 194 218 – 299 217 194 236 246 200 183 210 96 111 161 64 38 28 16 25 39
Peroxisomes 12 12 59 86 103 119 – 111 82 56 78 82 38 41 52 13 10 14 5 3 3 7 11 9
SpindlePole 272 148 353 194 239 249 – 337 213 249 236 243 166 166 188 54 46 86 349 186 371 126 131 169
Vac/Vac Membrane 5 1 0 1 2 0 – 1 1 0 0 1 2 1 2 2 3 4 1 1 1 2 3 2
Unique Cell Count 503 264 477 345 390 413 604 379 382 436 449 300 320 360 141 138 218 563 292 495 219 259 361
Labelled Cell Count 758 420 756 560 617 676 887 614 593 670 686 482 477 550 214 212 321 563 292 495 219 259 361


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 10.1 6.0 7.1 8.2 7.2 – 7.0 6.4 6.7 6.6 6.8 9.1 9.1 8.8 11.9 12.4 12.5 8.3 8.4 9.3
Std Deviation (1e-4) 2.2 2.2 1.5 2.0 4.5 2.6 – 2.3 1.9 2.3 2.0 2.0 2.3 3.0 2.6 4.1 3.6 3.9 1.9 2.1 1.8
Intensity Change (Log2) – – – 0.25 0.45 0.27 – 0.23 0.08 0.15 0.13 0.18 0.6 0.61 0.55 0.98 1.05 1.05 0.46 0.48 0.62

WT3RAP60RAP140RAP220RAP380RAP460RAP540RAP620RAP700051015WT3HU80HU120HU160051015WT3rpd3Δ_1rpd3Δ_2rpd3Δ_3051015WT1AF100AF140AF180051015
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud -1.9 -1.8 -1.9 – 0.7 -0.7 -0.7 0.4 -0.2 -3.1 -3.2 -3.4 0 0 -2.2
Bud Neck 0 -0.4 0.6 – 0.3 0.2 0.2 2.3 -0.1 -0.6 -0.7 -1.3 0 0 0
Bud Site -0.4 -2.3 -1.2 – 1.1 2.0 3.1 1.7 2.4 -3.0 -2.8 -3.0 0 0 -2.9
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.3 0 – 0 0 0 0 0 5.8 7.2 7.9 0 0 6.2
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 5.1 2.1 – -0.7 0 0 -0.8 0.4 5.9 4.5 5.5 0 0 2.8
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.5 -4.7 -0.9 – -2.0 -0.2 -1.4 -0.9 -1.0 -4.5 -4.7 -5.5 -0.6 -0.5 -2.9
Nucleus -0.3 -1.1 -0.1 – -0.9 0.7 -0.3 -1.4 -1.0 -1.3 -0.1 -2.6 0.4 1.2 1.4
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.9 0.6 1.5 – 0.6 2.8 0.9 1.9 2.1 5.1 2.6 3.0 4.2 6.8 6.4
Peroxisomes 4.7 5.3 6.1 – 2.7 3.6 1.0 2.3 2.5 0.1 0.2 0.9 -1.0 -1.7 -2.4
SpindlePole -5.3 -4.0 -4.4 – -6.2 -5.5 -2.8 -6.3 -6.3 -5.4 -6.4 -6.5 -7.9 -8.8 -8.8
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2612 5.5172 5.5289 4.9827 5.5419 5.4357 5.3338 4.6392 5.027 4.6793 3.8285 4.6362 7.1679 7.004 6.5044 7.3134 6.2429 6.641
Actin 0.0578 0 0.0037 0.0085 0.0016 0.0073 0.0035 0 0.018 0.0001 0 0.0003 0.0479 0 0.0256 0.0001 0.0267 0
Bud 0.0008 0.0001 0.0003 0.0009 0.0002 0.0002 0.0002 0.0001 0.0002 0 0 0 0.0003 0.0002 0.0005 0.0003 0.0024 0
Bud Neck 0.0018 0.0003 0.0056 0.0225 0.0006 0.0007 0.0005 0.0003 0.0009 0.0002 0 0.0004 0.0032 0.0013 0.0005 0.0011 0.001 0.0003
Bud Periphery 0.0011 0.0001 0.0004 0.0014 0.0006 0.0003 0.0002 0.0001 0.0004 0 0 0.0001 0.0004 0.0003 0.0007 0.0002 0.0061 0
Bud Site 0.0077 0.0004 0.0016 0.0025 0.0004 0.002 0.0024 0.0009 0.0015 0.0001 0 0.0001 0.0014 0.0012 0.0033 0.0024 0.0033 0.0001
Cell Periphery 0.0004 0 0.0002 0.0003 0.0002 0.0001 0.0001 0 0.0001 0 0 0 0.0001 0.0002 0.0001 0.0001 0.0008 0
Cytoplasm 0.0171 0.0001 0.0008 0.0023 0.0003 0.0003 0.0067 0 0.001 0 0 0.0001 0.0031 0.0003 0.0003 0.0022 0.001 0
Cytoplasmic Foci 0.0424 0.0055 0.0159 0.0515 0.0107 0.005 0.0159 0.0007 0.0032 0.0076 0.0017 0.0013 0.0258 0.0063 0.0054 0.0137 0.0045 0.0016
Eisosomes 0.0008 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0.0003 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0083 0 0.0004 0.0015 0.0007 0.0008 0.0035 0 0.0014 0 0 0.0004 0.0018 0.0002 0.0003 0.0002 0.0045 0
Endosome 0.0523 0.0001 0.0092 0.0426 0.0024 0.0056 0.0091 0 0.0058 0.0003 0 0.0013 0.0145 0.0016 0.0033 0.0007 0.0365 0
Golgi 0.0146 0.0002 0.0071 0.017 0.0023 0.0044 0.0038 0 0.003 0.0007 0 0.0005 0.008 0.0001 0.0028 0.0003 0.0099 0.0001
Lipid Particles 0.0363 0.0017 0.0206 0.0645 0.0253 0.0101 0.0119 0.0001 0.004 0.0232 0.0004 0.0029 0.0144 0.0004 0.0043 0.0014 0.0097 0.0011
Mitochondria 0.0393 0.0001 0.0169 0.0112 0.0031 0.004 0.0042 0.0002 0.012 0.0008 0 0.0004 0.002 0.0002 0.0127 0.001 0.0104 0
None 0.0104 0 0.0002 0.0026 0.0005 0.0002 0.0013 0 0.0004 0.0001 0 0.0001 0.0062 0.0001 0.0004 0.0011 0.002 0
Nuclear Periphery 0.0275 0.0006 0.0032 0.0068 0.0179 0.0101 0.0142 0.0012 0.0099 0.0005 0.0004 0.0119 0.0312 0.001 0.0026 0.0015 0.0374 0.0003
Nucleolus 0.0334 0.166 0.1815 0.0968 0.1438 0.1195 0.0674 0.1458 0.0696 0.0714 0.0057 0.106 0.0926 0.1859 0.1854 0.1937 0.1921 0.0746
Nucleus 0.0354 0.0816 0.0406 0.0189 0.0172 0.028 0.0188 0.088 0.0366 0.0311 0.0011 0.0264 0.0222 0.1436 0.0553 0.0813 0.0526 0.0122
Peroxisomes 0.0163 0.0009 0.0035 0.0128 0.001 0.0021 0.0027 0 0.0006 0.0023 0 0.0002 0.0183 0 0.0044 0.0004 0.0023 0.0001
Punctate Nuclear 0.5833 0.7419 0.6868 0.6164 0.7675 0.798 0.8319 0.7622 0.8296 0.8612 0.9906 0.8473 0.7049 0.648 0.6826 0.6972 0.5708 0.9096
Vacuole 0.0086 0.0003 0.0009 0.0117 0.0011 0.0005 0.0009 0.0001 0.0005 0.0001 0 0.0002 0.0005 0.0089 0.0056 0.0008 0.0187 0
Vacuole Periphery 0.0043 0.0001 0.0006 0.0071 0.0025 0.0007 0.0008 0.0001 0.001 0.0001 0 0.0003 0.0008 0.0003 0.0039 0.0004 0.0071 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.1012 3.461 17.9773 17.2175 12.5492 30.9556 27.9951 46.056 36.9859 27.7875
Translational Efficiency 1.0079 11.9073 1.103 1.1034 2.0283 1.1796 1.2841 0.7922 0.7593 0.7956

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
38 1247 158 950 1154 1538 4 1230 1192 2785 162 2180

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 947.85 971.23 1224.94 990.12 693.77 951.92 547.94 998.63 701.87 960.57 1208.22 994.92
Standard Deviation 135.51 169.94 152.03 174.08 163.21 157.06 125.76 172.51 168.42 163.24 184.31 173.25
Intensity Change Log 2 — 0.035154 0.369980 0.062945 — 0.456383 -0.340440 0.525493 — 0.228351 0.110975 0.276742

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000875 0.000217 0.000214 0.000514 0.000811 0.000354 0.004284 0.000241 0.000813 0.000292 0.000314 0.000360
Bud Neck 0.002003 0.001110 0.001402 0.007421 0.003220 0.001996 0.000978 0.002351 0.003181 0.001599 0.001391 0.004561
Bud Site 0.001824 0.000772 0.000886 0.004867 0.004356 0.001413 0.002155 0.001765 0.004276 0.001126 0.000918 0.003117
Cell Periphery 0.000055 0.000042 0.000018 0.000074 0.000270 0.000073 0.003797 0.000087 0.000263 0.000059 0.000111 0.000081
Cytoplasm 0.001124 0.000453 0.000075 0.011556 0.006948 0.001795 0.109401 0.002151 0.006762 0.001194 0.002775 0.006250
Cytoplasmic Foci 0.006122 0.008733 0.004676 0.005744 0.025871 0.007510 0.002753 0.003016 0.025241 0.008058 0.004629 0.004205
Eisosomes 0.000066 0.000145 0.000046 0.000024 0.000297 0.000110 0.000419 0.000017 0.000290 0.000126 0.000055 0.000020
Endoplasmic Reticulum 0.003940 0.001048 0.000741 0.000898 0.004006 0.001310 0.121893 0.000439 0.004004 0.001193 0.003733 0.000639
Endosome 0.000505 0.000630 0.000083 0.003313 0.003917 0.001698 0.002062 0.002029 0.003808 0.001220 0.000132 0.002589
Golgi 0.001302 0.001814 0.000481 0.001747 0.007881 0.001650 0.001153 0.001312 0.007671 0.001724 0.000497 0.001502
Lipid Particles 0.010917 0.002621 0.000192 0.001507 0.009263 0.002579 0.002729 0.000556 0.009315 0.002598 0.000255 0.000970
Mitochondria 0.006541 0.001678 0.000447 0.006954 0.001873 0.002020 0.005502 0.001206 0.002022 0.001867 0.000571 0.003711
Mitotic Spindle 0.851124 0.781794 0.885796 0.854317 0.501133 0.735227 0.000016 0.910093 0.512290 0.756078 0.863925 0.885787
None 0.000269 0.001095 0.000100 0.004336 0.020397 0.003187 0.261212 0.000770 0.019755 0.002251 0.006547 0.002324
Nuclear Periphery 0.000631 0.000442 0.000239 0.001109 0.000546 0.000661 0.002844 0.000423 0.000549 0.000563 0.000303 0.000722
Nuclear Periphery Foci 0.005843 0.017710 0.001745 0.003746 0.102685 0.015708 0.026260 0.002625 0.099598 0.016605 0.002350 0.003114
Nucleolus 0.060692 0.106509 0.058396 0.038775 0.201812 0.130417 0.005707 0.034943 0.197313 0.119712 0.057095 0.036613
Nucleus 0.017663 0.031589 0.015750 0.040361 0.047786 0.061573 0.436259 0.028088 0.046826 0.048147 0.026133 0.033436
Peroxisomes 0.026903 0.040734 0.028128 0.006967 0.055179 0.029067 0.000893 0.004928 0.054278 0.034291 0.027456 0.005816
Vacuole 0.001006 0.000681 0.000467 0.004243 0.001515 0.001339 0.006515 0.002432 0.001498 0.001044 0.000616 0.003221
Vacuole Periphery 0.000594 0.000183 0.000118 0.001527 0.000234 0.000311 0.003167 0.000527 0.000246 0.000254 0.000193 0.000963

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.34 1.14 0.35 -3.82 -2.41 NaN NaN NaN NaN NaN 5.36 2.55 2.87 -3.19 -0.64
Bud Neck 2.32 1.54 -2.05 -5.58 -4.57 NaN NaN NaN NaN NaN 3.16 1.02 -4.11 -6.63 -4.67
Bud Site 2.10 1.52 -0.66 -6.58 -4.84 NaN NaN NaN NaN NaN 5.97 3.35 -0.61 -7.42 -4.20
Cell Periphery 0.93 1.22 -0.82 -4.08 -4.05 NaN NaN NaN NaN NaN 10.13 2.58 5.68 -1.05 0.74
Cytoplasm 0.53 1.14 -4.62 -4.95 -5.13 NaN NaN NaN NaN NaN 5.34 1.65 -0.14 -5.10 -1.71
Cytoplasmic Foci -1.04 -0.26 0.01 2.04 0.41 NaN NaN NaN NaN NaN 7.39 6.25 9.34 4.35 1.25
Eisosomes -1.97 1.29 2.77 11.20 3.18 NaN NaN NaN NaN NaN 11.35 11.85 20.54 17.49 3.56
Endoplasmic Reticulum 0.99 1.10 0.95 -0.40 -0.73 NaN NaN NaN NaN NaN 11.37 0.13 12.11 2.69 0.99
Endosome 0.62 2.66 -2.91 -3.18 -4.15 NaN NaN NaN NaN NaN 4.99 7.20 2.61 -2.42 -5.01
Golgi 0.12 2.08 0.46 0.44 -1.47 NaN NaN NaN NaN NaN 9.28 10.56 9.37 1.10 -1.85
Lipid Particles 0.70 1.02 0.87 1.33 -1.75 NaN NaN NaN NaN NaN 5.85 7.95 7.44 3.09 -1.59
Mitochondria 0.70 0.92 -0.23 -2.50 -3.22 NaN NaN NaN NaN NaN 0.28 2.57 -1.72 -1.94 -3.39
Mitotic Spindle 0.15 -0.95 -0.75 -3.35 0.58 NaN NaN NaN NaN NaN -19.33 -12.21 -27.39 -11.79 -1.69
None -2.70 0.89 -3.04 -2.35 -3.19 NaN NaN NaN NaN NaN 8.38 2.31 8.26 -0.11 0.70
Nuclear Periphery 1.00 1.42 -1.51 -4.47 -4.81 NaN NaN NaN NaN NaN -0.66 1.44 -3.52 -3.18 -3.66
Nuclear Periphery Foci -3.95 1.08 1.39 6.55 -0.15 NaN NaN NaN NaN NaN 16.28 17.56 19.41 10.35 0.23
Nucleolus -0.07 0.76 1.94 8.02 2.19 NaN NaN NaN NaN NaN 9.63 8.10 19.62 15.48 2.44
Nucleus -0.37 0.32 -2.52 -3.95 -3.79 NaN NaN NaN NaN NaN -0.87 1.01 -0.20 0.56 -1.08
Peroxisomes -0.52 -0.05 2.66 13.14 4.74 NaN NaN NaN NaN NaN 5.73 2.61 17.09 18.56 4.91
Vacuole 1.84 1.23 -3.39 -4.24 -4.01 NaN NaN NaN NaN NaN 1.81 1.28 -4.03 -4.49 -4.33
Vacuole Periphery 1.17 1.19 -1.21 -2.34 -2.36 NaN NaN NaN NaN NaN -0.32 -0.20 -2.65 -2.55 -2.43
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
Localization
Cell Percentages spindle pole (33%), nucleolus (8%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Dad1

Dad1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dad1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available