Standard name
Human Ortholog
Description Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0.05 0.07 0.09 0.06 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0.09 0.12 0.14 0.11 0.09 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.09 0 – 0 0 0 0 0 0.11 0.08 0.1 0.09 0.07 0.05 0.06 0 0.05 0.07 0.06 0.07
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.05 0.09 0.08 0 0.08 – 0.06 0.09 0.07 0.08 0.08 0 0 0 0.08 0.08 0 0 0 0 0 0.06 0.06
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.76 0.83 0.48 0.61 0.5 0.53 – 0.5 0.57 0.51 0.54 0.55 0.67 0.57 0.58 0.68 0.8 0.74 0.12 0.13 0.06 0.07 0.1 0.11
Peroxisomes 0 0.05 0.12 0.25 0.26 0.29 – 0.18 0.22 0.15 0.18 0.18 0.13 0.13 0.14 0.09 0.07 0.06 0 0 0 0 0 0
SpindlePole 0.54 0.56 0.74 0.56 0.61 0.6 – 0.56 0.56 0.65 0.54 0.54 0.55 0.52 0.52 0.38 0.33 0.39 0.62 0.64 0.75 0.58 0.51 0.47
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 1
Bud 0 0 15 4 5 5 – 24 9 9 16 13 0 0 0 0 0 1 5 2 3 1 5 3
Bud Neck 5 4 9 2 6 10 – 13 8 8 20 8 4 4 3 0 1 1 8 4 2 1 2 4
Bud Site 7 0 18 11 5 10 – 31 26 34 27 33 1 2 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 5 3 5 10 2 – 8 2 4 7 6 26 39 51 15 12 22 0 0 0 0 1 1
Endoplasmic Reticulum 1 0 0 0 0 2 – 0 0 0 1 0 0 0 1 2 0 1 0 0 0 0 0 0
Endosome 9 10 7 5 35 15 – 6 3 0 4 8 33 25 36 13 9 11 36 11 22 15 16 23
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 3 2 1 0 2 0
Mitochondria 37 13 45 29 7 32 – 37 34 26 34 34 4 4 2 11 11 7 17 5 8 7 15 22
Nucleus 20 6 17 11 9 14 – 16 17 12 9 11 6 11 3 6 8 13 11 3 5 4 2 2
Nuclear Periphery 4 1 2 0 1 0 – 4 2 1 2 1 1 1 0 1 1 0 2 0 0 1 1 1
Nucleolus 382 220 228 212 194 218 – 299 217 194 236 246 200 183 210 96 111 161 64 38 28 16 25 39
Peroxisomes 12 12 59 86 103 119 – 111 82 56 78 82 38 41 52 13 10 14 5 3 3 7 11 9
SpindlePole 272 148 353 194 239 249 – 337 213 249 236 243 166 166 188 54 46 86 349 186 371 126 131 169
Vac/Vac Membrane 5 1 0 1 2 0 – 1 1 0 0 1 2 1 2 2 3 4 1 1 1 2 3 2
Unique Cell Count 503 264 477 345 390 413 604 379 382 436 449 300 320 360 141 138 218 563 292 495 219 259 361
Labelled Cell Count 758 420 756 560 617 676 887 614 593 670 686 482 477 550 214 212 321 563 292 495 219 259 361


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.9 10.1 6.0 7.1 8.2 7.2 – 7.0 6.4 6.7 6.6 6.8 9.1 9.1 8.8 11.9 12.4 12.5 8.3 8.4 9.3
Std Deviation (1e-4) 2.2 2.2 1.5 2.0 4.5 2.6 – 2.3 1.9 2.3 2.0 2.0 2.3 3.0 2.6 4.1 3.6 3.9 1.9 2.1 1.8
Intensity Change (Log2) – – – 0.25 0.45 0.27 – 0.23 0.08 0.15 0.13 0.18 0.6 0.61 0.55 0.98 1.05 1.05 0.46 0.48 0.62


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud -1.9 -1.8 -1.9 – 0.7 -0.7 -0.7 0.4 -0.2 -3.1 -3.2 -3.4 0 0 -2.2
Bud Neck 0 -0.4 0.6 – 0.3 0.2 0.2 2.3 -0.1 -0.6 -0.7 -1.3 0 0 0
Bud Site -0.4 -2.3 -1.2 – 1.1 2.0 3.1 1.7 2.4 -3.0 -2.8 -3.0 0 0 -2.9
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.3 0 – 0 0 0 0 0 5.8 7.2 7.9 0 0 6.2
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 5.1 2.1 – -0.7 0 0 -0.8 0.4 5.9 4.5 5.5 0 0 2.8
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.5 -4.7 -0.9 – -2.0 -0.2 -1.4 -0.9 -1.0 -4.5 -4.7 -5.5 -0.6 -0.5 -2.9
Nucleus -0.3 -1.1 -0.1 – -0.9 0.7 -0.3 -1.4 -1.0 -1.3 -0.1 -2.6 0.4 1.2 1.4
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.9 0.6 1.5 – 0.6 2.8 0.9 1.9 2.1 5.1 2.6 3.0 4.2 6.8 6.4
Peroxisomes 4.7 5.3 6.1 – 2.7 3.6 1.0 2.3 2.5 0.1 0.2 0.9 -1.0 -1.7 -2.4
SpindlePole -5.3 -4.0 -4.4 – -6.2 -5.5 -2.8 -6.3 -6.3 -5.4 -6.4 -6.5 -7.9 -8.8 -8.8
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2612 5.5172 5.5289 4.9827 5.5419 5.4357 5.3338 4.6392 5.027 4.6793 3.8285 4.6362 7.1679 7.004 6.5044 7.3134 6.2429 6.641
Actin 0.0578 0 0.0037 0.0085 0.0016 0.0073 0.0035 0 0.018 0.0001 0 0.0003 0.0479 0 0.0256 0.0001 0.0267 0
Bud 0.0008 0.0001 0.0003 0.0009 0.0002 0.0002 0.0002 0.0001 0.0002 0 0 0 0.0003 0.0002 0.0005 0.0003 0.0024 0
Bud Neck 0.0018 0.0003 0.0056 0.0225 0.0006 0.0007 0.0005 0.0003 0.0009 0.0002 0 0.0004 0.0032 0.0013 0.0005 0.0011 0.001 0.0003
Bud Periphery 0.0011 0.0001 0.0004 0.0014 0.0006 0.0003 0.0002 0.0001 0.0004 0 0 0.0001 0.0004 0.0003 0.0007 0.0002 0.0061 0
Bud Site 0.0077 0.0004 0.0016 0.0025 0.0004 0.002 0.0024 0.0009 0.0015 0.0001 0 0.0001 0.0014 0.0012 0.0033 0.0024 0.0033 0.0001
Cell Periphery 0.0004 0 0.0002 0.0003 0.0002 0.0001 0.0001 0 0.0001 0 0 0 0.0001 0.0002 0.0001 0.0001 0.0008 0
Cytoplasm 0.0171 0.0001 0.0008 0.0023 0.0003 0.0003 0.0067 0 0.001 0 0 0.0001 0.0031 0.0003 0.0003 0.0022 0.001 0
Cytoplasmic Foci 0.0424 0.0055 0.0159 0.0515 0.0107 0.005 0.0159 0.0007 0.0032 0.0076 0.0017 0.0013 0.0258 0.0063 0.0054 0.0137 0.0045 0.0016
Eisosomes 0.0008 0 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0.0003 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0083 0 0.0004 0.0015 0.0007 0.0008 0.0035 0 0.0014 0 0 0.0004 0.0018 0.0002 0.0003 0.0002 0.0045 0
Endosome 0.0523 0.0001 0.0092 0.0426 0.0024 0.0056 0.0091 0 0.0058 0.0003 0 0.0013 0.0145 0.0016 0.0033 0.0007 0.0365 0
Golgi 0.0146 0.0002 0.0071 0.017 0.0023 0.0044 0.0038 0 0.003 0.0007 0 0.0005 0.008 0.0001 0.0028 0.0003 0.0099 0.0001
Lipid Particles 0.0363 0.0017 0.0206 0.0645 0.0253 0.0101 0.0119 0.0001 0.004 0.0232 0.0004 0.0029 0.0144 0.0004 0.0043 0.0014 0.0097 0.0011
Mitochondria 0.0393 0.0001 0.0169 0.0112 0.0031 0.004 0.0042 0.0002 0.012 0.0008 0 0.0004 0.002 0.0002 0.0127 0.001 0.0104 0
None 0.0104 0 0.0002 0.0026 0.0005 0.0002 0.0013 0 0.0004 0.0001 0 0.0001 0.0062 0.0001 0.0004 0.0011 0.002 0
Nuclear Periphery 0.0275 0.0006 0.0032 0.0068 0.0179 0.0101 0.0142 0.0012 0.0099 0.0005 0.0004 0.0119 0.0312 0.001 0.0026 0.0015 0.0374 0.0003
Nucleolus 0.0334 0.166 0.1815 0.0968 0.1438 0.1195 0.0674 0.1458 0.0696 0.0714 0.0057 0.106 0.0926 0.1859 0.1854 0.1937 0.1921 0.0746
Nucleus 0.0354 0.0816 0.0406 0.0189 0.0172 0.028 0.0188 0.088 0.0366 0.0311 0.0011 0.0264 0.0222 0.1436 0.0553 0.0813 0.0526 0.0122
Peroxisomes 0.0163 0.0009 0.0035 0.0128 0.001 0.0021 0.0027 0 0.0006 0.0023 0 0.0002 0.0183 0 0.0044 0.0004 0.0023 0.0001
Punctate Nuclear 0.5833 0.7419 0.6868 0.6164 0.7675 0.798 0.8319 0.7622 0.8296 0.8612 0.9906 0.8473 0.7049 0.648 0.6826 0.6972 0.5708 0.9096
Vacuole 0.0086 0.0003 0.0009 0.0117 0.0011 0.0005 0.0009 0.0001 0.0005 0.0001 0 0.0002 0.0005 0.0089 0.0056 0.0008 0.0187 0
Vacuole Periphery 0.0043 0.0001 0.0006 0.0071 0.0025 0.0007 0.0008 0.0001 0.001 0.0001 0 0.0003 0.0008 0.0003 0.0039 0.0004 0.0071 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.1012 3.461 17.9773 17.2175 12.5492 30.9556 27.9951 46.056 36.9859 27.7875
Translational Efficiency 1.0079 11.9073 1.103 1.1034 2.0283 1.1796 1.2841 0.7922 0.7593 0.7956

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
38 1247 158 950 1154 1538 4 1230 1192 2785 162 2180

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 947.85 971.23 1224.94 990.12 693.77 951.92 547.94 998.63 701.87 960.57 1208.22 994.92
Standard Deviation 135.51 169.94 152.03 174.08 163.21 157.06 125.76 172.51 168.42 163.24 184.31 173.25
Intensity Change Log 2 — 0.035154 0.369980 0.062945 — 0.456383 -0.340440 0.525493 — 0.228351 0.110975 0.276742

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000875 0.000217 0.000214 0.000514 0.000811 0.000354 0.004284 0.000241 0.000813 0.000292 0.000314 0.000360
Bud Neck 0.002003 0.001110 0.001402 0.007421 0.003220 0.001996 0.000978 0.002351 0.003181 0.001599 0.001391 0.004561
Bud Site 0.001824 0.000772 0.000886 0.004867 0.004356 0.001413 0.002155 0.001765 0.004276 0.001126 0.000918 0.003117
Cell Periphery 0.000055 0.000042 0.000018 0.000074 0.000270 0.000073 0.003797 0.000087 0.000263 0.000059 0.000111 0.000081
Cytoplasm 0.001124 0.000453 0.000075 0.011556 0.006948 0.001795 0.109401* 0.002151 0.006762 0.001194 0.002775 0.006250
Cytoplasmic Foci 0.006122 0.008733 0.004676 0.005744 0.025871 0.007510 0.002753 0.003016 0.025241 0.008058 0.004629 0.004205
Eisosomes 0.000066 0.000145 0.000046 0.000024 0.000297 0.000110 0.000419 0.000017 0.000290 0.000126 0.000055 0.000020
Endoplasmic Reticulum 0.003940 0.001048 0.000741 0.000898 0.004006 0.001310 0.121893* 0.000439 0.004004 0.001193 0.003733 0.000639
Endosome 0.000505 0.000630 0.000083 0.003313 0.003917 0.001698 0.002062 0.002029 0.003808 0.001220 0.000132 0.002589
Golgi 0.001302 0.001814 0.000481 0.001747 0.007881 0.001650 0.001153 0.001312 0.007671 0.001724 0.000497 0.001502
Lipid Particles 0.010917 0.002621 0.000192 0.001507 0.009263 0.002579 0.002729 0.000556 0.009315 0.002598 0.000255 0.000970
Mitochondria 0.006541 0.001678 0.000447 0.006954 0.001873 0.002020 0.005502 0.001206 0.002022 0.001867 0.000571 0.003711
Mitotic Spindle 0.851124* 0.781794* 0.885796* 0.854317* 0.501133* 0.735227* 0.000016 0.910093* 0.512290* 0.756078* 0.863925* 0.885787*
None 0.000269 0.001095 0.000100 0.004336 0.020397 0.003187 0.261212* 0.000770 0.019755 0.002251 0.006547 0.002324
Nuclear Periphery 0.000631 0.000442 0.000239 0.001109 0.000546 0.000661 0.002844 0.000423 0.000549 0.000563 0.000303 0.000722
Nuclear Periphery Foci 0.005843 0.017710 0.001745 0.003746 0.102685 0.015708 0.026260 0.002625 0.099598 0.016605 0.002350 0.003114
Nucleolus 0.060692 0.106509 0.058396 0.038775 0.201812 0.130417 0.005707 0.034943 0.197313 0.119712 0.057095 0.036613
Nucleus 0.017663 0.031589 0.015750 0.040361 0.047786 0.061573 0.436259* 0.028088 0.046826 0.048147 0.026133 0.033436
Peroxisomes 0.026903 0.040734 0.028128 0.006967 0.055179 0.029067 0.000893 0.004928 0.054278 0.034291 0.027456 0.005816
Vacuole 0.001006 0.000681 0.000467 0.004243 0.001515 0.001339 0.006515 0.002432 0.001498 0.001044 0.000616 0.003221
Vacuole Periphery 0.000594 0.000183 0.000118 0.001527 0.000234 0.000311 0.003167 0.000527 0.000246 0.000254 0.000193 0.000963

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.34 1.14 0.35 -3.82 -2.41 NaN NaN NaN NaN NaN 5.36 2.55 2.87 -3.19 -0.64
Bud Neck 2.32 1.54 -2.05 -5.58 -4.57 NaN NaN NaN NaN NaN 3.16 1.02 -4.11 -6.63 -4.67
Bud Site 2.10 1.52 -0.66 -6.58 -4.84 NaN NaN NaN NaN NaN 5.97 3.35 -0.61 -7.42 -4.20
Cell Periphery 0.93 1.22 -0.82 -4.08 -4.05 NaN NaN NaN NaN NaN 10.13 2.58 5.68 -1.05 0.74
Cytoplasm 0.53 1.14 -4.62 -4.95 -5.13 NaN NaN NaN NaN NaN 5.34 1.65 -0.14 -5.10 -1.71
Cytoplasmic Foci -1.04 -0.26 0.01 2.04 0.41 NaN NaN NaN NaN NaN 7.39 6.25 9.34 4.35 1.25
Eisosomes -1.97 1.29 2.77 11.20 3.18 NaN NaN NaN NaN NaN 11.35 11.85 20.54 17.49 3.56
Endoplasmic Reticulum 0.99 1.10 0.95 -0.40 -0.73 NaN NaN NaN NaN NaN 11.37 0.13 12.11 2.69 0.99
Endosome 0.62 2.66 -2.91 -3.18 -4.15 NaN NaN NaN NaN NaN 4.99 7.20 2.61 -2.42 -5.01
Golgi 0.12 2.08 0.46 0.44 -1.47 NaN NaN NaN NaN NaN 9.28 10.56 9.37 1.10 -1.85
Lipid Particles 0.70 1.02 0.87 1.33 -1.75 NaN NaN NaN NaN NaN 5.85 7.95 7.44 3.09 -1.59
Mitochondria 0.70 0.92 -0.23 -2.50 -3.22 NaN NaN NaN NaN NaN 0.28 2.57 -1.72 -1.94 -3.39
Mitotic Spindle 0.15 -0.95 -0.75 -3.35 0.58 NaN NaN NaN NaN NaN -19.33 -12.21 -27.39 -11.79 -1.69
None -2.70 0.89 -3.04 -2.35 -3.19 NaN NaN NaN NaN NaN 8.38 2.31 8.26 -0.11 0.70
Nuclear Periphery 1.00 1.42 -1.51 -4.47 -4.81 NaN NaN NaN NaN NaN -0.66 1.44 -3.52 -3.18 -3.66
Nuclear Periphery Foci -3.95 1.08 1.39 6.55 -0.15 NaN NaN NaN NaN NaN 16.28 17.56 19.41 10.35 0.23
Nucleolus -0.07 0.76 1.94 8.02 2.19 NaN NaN NaN NaN NaN 9.63 8.10 19.62 15.48 2.44
Nucleus -0.37 0.32 -2.52 -3.95 -3.79 NaN NaN NaN NaN NaN -0.87 1.01 -0.20 0.56 -1.08
Peroxisomes -0.52 -0.05 2.66 13.14 4.74 NaN NaN NaN NaN NaN 5.73 2.61 17.09 18.56 4.91
Vacuole 1.84 1.23 -3.39 -4.24 -4.01 NaN NaN NaN NaN NaN 1.81 1.28 -4.03 -4.49 -4.33
Vacuole Periphery 1.17 1.19 -1.21 -2.34 -2.36 NaN NaN NaN NaN NaN -0.32 -0.20 -2.65 -2.55 -2.43
Standard name
Human Ortholog
Description Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
Localization
Cell Percentages spindle pole (33%), nucleolus (8%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Dad1

Dad1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dad1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available