Standard name
Human Ortholog
Description Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.96 0.91 0.88 0.75 – 0.61 0.54 0.55 0.52 0.52 0.99 0.98 0.99 0.91 0.95 0.93 0.9 0.87 0.86 0.91 0.86 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0.1 0.34 0.18 0.43 – 0.5 0.67 0.62 0.69 0.64 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0.06 0.07 0 0 0 0 0 0 0.11 0 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 2 3 5 0 1 0
Bud 1 0 6 3 5 3 – 16 11 19 16 17 0 0 0 0 0 0 2 1 4 0 2 8
Bud Neck 2 0 3 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 0 1
Bud Site 0 0 0 0 0 0 – 1 0 0 2 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 4 5 1 – 1 3 0 3 2 0 0 0 10 7 6 0 0 1 0 0 0
Cytoplasm 350 255 452 428 510 415 – 397 214 275 168 230 140 127 142 230 292 230 345 241 390 104 113 154
Endoplasmic Reticulum 2 1 1 0 1 9 – 0 0 1 1 1 1 0 1 3 9 0 0 2 2 0 0 1
Endosome 0 0 0 1 13 5 – 6 2 3 0 2 0 0 1 1 1 3 5 3 3 0 1 5
Golgi 0 0 2 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 1 3 0 0 0
Mitochondria 17 1 48 157 107 236 – 329 266 309 225 285 3 3 0 5 5 1 7 7 24 3 2 3
Nucleus 1 1 0 0 1 0 – 14 2 2 4 1 0 0 0 2 7 1 0 0 1 0 0 0
Nuclear Periphery 0 1 0 0 0 0 – 4 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 1 1 0 – 2 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 1 2 0 – 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 1 2 0 – 2 2 2 1 1 0 1 0 0 0 0 1 2 3 0 2 1
Vac/Vac Membrane 1 2 0 0 17 17 – 42 27 19 12 11 0 0 0 28 9 29 9 6 3 1 2 3
Unique Cell Count 362 259 469 468 579 551 652 397 498 325 445 142 129 143 252 307 248 382 277 454 115 131 185
Labelled Cell Count 375 261 515 596 664 686 814 528 634 433 554 144 131 144 280 330 270 382 277 454 115 131 185


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 5.2 3.7 3.7 4.5 3.9 – 4.0 3.5 3.7 3.6 3.5 5.1 5.2 5.3 8.2 7.5 7.8 4.9 5.7 5.7
Std Deviation (1e-4) 0.5 0.7 1.2 1.3 1.1 0.9 – 1.4 1.0 0.9 1.0 0.9 0.6 0.8 0.9 1.7 1.6 1.8 1.4 2.0 1.9
Intensity Change (Log2) – – – -0.03 0.25 0.05 – 0.09 -0.08 -0.02 -0.06 -0.11 0.44 0.47 0.5 1.14 1.0 1.06 0.39 0.61 0.61


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -3.2 -4.9 -9.4 – -13.7 -14.8 -14.8 -15.0 -15.5 1.3 1.2 1.8 -2.9 -0.9 -2.1
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 -3.1 -2.8 -4.0 -4.0 -4.6 -5.0
Nucleus 0 0 0 – 3.2 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.7 3.8 – 5.6 5.7 4.3 0 3.4 0 0 0 7.4 0 7.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6733 0.8801 0.6571 0.7559 0.1024 0.8688 0.4218 0.6688 0.5605 0.5298 0.2905 0.7827 2.4543 2.6709 2.4147 2.1717 2.3402 2.3817
Actin 0.0004 0.0004 0.001 0.0017 0.0015 0.0018 0.001 0.0024 0.0013 0.015 0.0003 0.0033 0.0008 0.0004 0.0005 0.0004 0.0096 0.0012
Bud 0.0011 0.0003 0.0001 0.0003 0.0003 0.0005 0.001 0.0023 0.0006 0.0003 0.0001 0.0006 0.0003 0.0002 0.0003 0.0001 0.0016 0.0005
Bud Neck 0.0001 0.0002 0.0002 0.0003 0.0003 0.0011 0.0007 0.0012 0.0011 0.031 0.0013 0.0061 0.0002 0.0003 0.0003 0.0003 0.0007 0.0027
Bud Periphery 0.0013 0.0001 0.0001 0.0003 0.0003 0.0004 0.0007 0.0022 0.0006 0.0005 0.0001 0.0006 0.0002 0.0002 0.0004 0.0001 0.0014 0.0005
Bud Site 0.0005 0.0013 0.0008 0.0002 0.0002 0.0001 0.0049 0.0089 0.002 0.0025 0.0004 0.0014 0.0015 0.0015 0.001 0.0001 0.0096 0.0006
Cell Periphery 0.0001 0.0001 0.0001 0.0001 0 0 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.5664 0.6268 0.5844 0.515 0.566 0.6738 0.4998 0.5772 0.5129 0.4582 0.4994 0.5364 0.4065 0.4463 0.3348 0.3331 0.3311 0.4809
Cytoplasmic Foci 0.0095 0.0071 0.0098 0.0079 0.0145 0.0117 0.0124 0.0122 0.0105 0.0066 0.0152 0.0163 0.0081 0.0106 0.0075 0.0059 0.0222 0.0141
Eisosomes 0.0001 0 0 0.0002 0 0 0.0001 0.0001 0.0001 0.0002 0 0 0.0001 0.0001 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0041 0.0022 0.003 0.0038 0.0023 0.0026 0.0038 0.0013 0.002 0.0022 0.0018 0.0017 0.0029 0.0018 0.0032 0.001 0.0011 0.0015
Endosome 0.0038 0.0029 0.0039 0.0047 0.0054 0.0031 0.0041 0.0023 0.0026 0.0026 0.0053 0.0042 0.004 0.0032 0.0031 0.0009 0.0093 0.0051
Golgi 0.0002 0.0002 0.0004 0.0003 0.0002 0.0001 0.0004 0.0002 0.0002 0.0002 0.0002 0.0002 0.0002 0.0003 0.0002 0.0001 0.0019 0.0002
Lipid Particles 0.0003 0.0001 0.0005 0.0004 0.0001 0.0001 0.0004 0.0001 0.0002 0.0001 0.0001 0.0003 0.0003 0.0006 0.0001 0.0001 0.002 0.0002
Mitochondria 0.0004 0.0002 0.0005 0.0004 0.0004 0.0002 0.0007 0.0005 0.0005 0.0004 0.0004 0.0004 0.0003 0.0006 0.0006 0.0003 0.0014 0.0005
None 0.4022 0.3493 0.3846 0.4561 0.3989 0.2934 0.4566 0.3741 0.4484 0.4681 0.4636 0.4091 0.562 0.52 0.6284 0.6504 0.5813 0.4745
Nuclear Periphery 0.0016 0.0013 0.0013 0.0018 0.0014 0.0011 0.0017 0.0009 0.0016 0.0022 0.001 0.0015 0.0017 0.0014 0.0024 0.0006 0.0009 0.0015
Nucleolus 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0.0004 0.0003 0.0003 0.0002 0.0003 0.0003 0.0003 0.0005 0.0002 0.0006 0.0003
Nucleus 0.0029 0.0043 0.0038 0.0033 0.0034 0.0059 0.0054 0.0082 0.0086 0.005 0.0057 0.0087 0.0067 0.0086 0.0093 0.0023 0.006 0.0084
Peroxisomes 0.0002 0.0001 0.0025 0.0002 0.0005 0.0003 0.0008 0.0005 0.0005 0.0004 0.0003 0.0005 0.0002 0.0006 0.0003 0.0002 0.0065 0.0005
Punctate Nuclear 0.0033 0.0013 0.002 0.0018 0.0027 0.0027 0.0035 0.0033 0.005 0.0029 0.0035 0.0074 0.0025 0.002 0.0058 0.0037 0.0108 0.0056
Vacuole 0.0012 0.0012 0.0007 0.0011 0.0011 0.0007 0.0012 0.0013 0.0009 0.001 0.001 0.0007 0.001 0.0009 0.0011 0.0002 0.0015 0.001
Vacuole Periphery 0.0002 0.0001 0.0001 0.0002 0.0003 0.0001 0.0002 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0004 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1.5074 5.39 3.9996 8.2609 11.7263 9.0393 8.1748 6.437 8.1602 10.7388
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
775 431 1788 961 1617 2004 2353 1724 2392 2435 4141 2685

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 593.35 748.54 923.24 979.88 672.92 735.18 898.36 950.41 647.14 737.54 909.10 960.96
Standard Deviation 79.37 83.33 113.78 135.04 76.61 94.23 115.88 135.09 85.99 92.53 115.63 135.81
Intensity Change Log 2 — 0.335196 0.637822 0.723722 — 0.127663 0.416859 0.498115 — 0.228634 0.524621 0.608233

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000177 0.000669 0.000166 0.000731 0.000149 0.000368 0.000091 0.001784 0.000158 0.000421 0.000123 0.001407
Bud Neck 0.022883 0.044599 0.002513 0.002811 0.018145 0.040743 0.006488 0.007121 0.019681 0.041426 0.004772 0.005579
Bud Site 0.003810 0.021055 0.007808 0.011818 0.004271 0.015681 0.009144 0.029745 0.004121 0.016632 0.008567 0.023329
Cell Periphery 0.000197 0.000287 0.000085 0.000120 0.000137 0.000124 0.000111 0.000097 0.000157 0.000153 0.000100 0.000106
Cytoplasm 0.386737* 0.319298* 0.478582* 0.562317* 0.447759* 0.430546* 0.565025* 0.587846* 0.427988* 0.410855* 0.527701* 0.578709*
Cytoplasmic Foci 0.304730* 0.336133* 0.006123 0.022509 0.278594* 0.257501* 0.014614 0.026933 0.287062* 0.271419* 0.010948 0.025350
Eisosomes 0.000284 0.000284 0.000020 0.000035 0.000230 0.000157 0.000039 0.000030 0.000248 0.000180 0.000031 0.000032
Endoplasmic Reticulum 0.000518 0.000534 0.002337 0.002737 0.000560 0.000460 0.000609 0.000858 0.000547 0.000473 0.001356 0.001531
Endosome 0.018336 0.032284 0.001279 0.008286 0.013218 0.023597 0.002283 0.012624 0.014876 0.025135 0.001849 0.011071
Golgi 0.002556 0.013067 0.000056 0.016182 0.002090 0.005417 0.000198 0.006956 0.002241 0.006771 0.000137 0.010258
Lipid Particles 0.019039 0.006381 0.000666 0.001626 0.012053 0.006270 0.000650 0.000844 0.014317 0.006290 0.000657 0.001124
Mitochondria 0.009382 0.016330 0.000602 0.002055 0.005857 0.014671 0.001453 0.005830 0.006999 0.014965 0.001085 0.004479
Mitotic Spindle 0.001308 0.007443 0.002072 0.028253 0.000566 0.006789 0.006765 0.057725 0.000807 0.006905 0.004738 0.047177
None 0.006875 0.000918 0.004768 0.008587 0.001705 0.002176 0.004516 0.003824 0.003380 0.001953 0.004625 0.005529
Nuclear Periphery 0.000890 0.000253 0.001442 0.001478 0.000476 0.000344 0.000802 0.000802 0.000610 0.000328 0.001078 0.001044
Nuclear Periphery Foci 0.000266 0.000415 0.001124 0.003478 0.000375 0.000250 0.000293 0.001030 0.000339 0.000279 0.000651 0.001906
Nucleolus 0.002333 0.001306 0.000179 0.000344 0.000827 0.001808 0.000332 0.000560 0.001315 0.001719 0.000266 0.000482
Nucleus 0.153651* 0.077609 0.445653* 0.131640* 0.154137* 0.096492 0.329722* 0.096649 0.153980* 0.093150 0.379779* 0.109173
Peroxisomes 0.011334 0.042615 0.000105 0.000960 0.006945 0.017420 0.000979 0.001886 0.008367 0.021879 0.000602 0.001555
Vacuole 0.053403 0.076366 0.044266 0.191882 0.051145 0.077090 0.055592 0.153825 0.051876 0.076962 0.050702 0.167446
Vacuole Periphery 0.001288 0.002155 0.000155 0.002151 0.000760 0.002096 0.000293 0.003031 0.000931 0.002107 0.000233 0.002716

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.17 -1.08 -2.76 1.99 -2.52 -4.47 0.80 -1.85 -1.16 -1.95 -6.15 -0.35 -2.28 -1.01 -2.25
Bud Neck -4.12 9.76 9.73 8.66 -0.44 -9.36 8.14 9.70 17.48 1.62 -10.00 12.70 13.61 19.82 1.10
Bud Site -6.74 -1.84 -2.66 5.30 -0.45 -8.05 -2.29 -6.08 0.42 -4.18 -10.26 -2.85 -6.59 2.98 -4.00
Cell Periphery -1.00 4.47 4.38 2.31 -0.17 1.48 6.57 9.37 6.50 4.14 0.34 7.24 8.65 4.58 3.23
Cytoplasm 4.37 -3.26 -2.27 -6.22 0.65 1.94 -5.50 -4.43 -6.28 0.58 2.22 -5.27 -4.61 -6.55 0.07
Cytoplasmic Foci -2.21 39.38 38.01 28.71 -8.91 2.97 47.71 46.38 47.51 -4.47 2.62 61.57 59.56 55.08 -8.43
Eisosomes 0.07 12.63 11.86 4.19 -7.51 4.57 13.15 13.48 17.63 1.89 4.19 18.03 17.75 12.23 -2.53
Endoplasmic Reticulum -0.36 -18.82 -9.76 -9.36 4.78 1.17 -7.18 -6.84 -8.40 0.55 1.06 -18.56 -11.68 -12.14 5.74
Endosome -3.73 10.51 9.17 8.77 -2.74 -6.36 10.13 6.77 14.00 -4.09 -6.96 14.37 10.72 17.15 -4.93
Golgi -4.26 4.35 -0.49 3.52 -2.37 -6.89 10.12 -0.04 4.64 -3.60 -7.48 9.46 -0.45 5.10 -4.05
Lipid Particles 6.31 9.65 9.30 8.05 -5.51 5.88 13.20 13.01 10.38 -2.98 8.50 16.03 15.63 11.81 -5.84
Mitochondria -2.04 5.05 4.99 5.53 -0.44 -5.06 4.59 3.50 8.20 -1.46 -5.09 6.90 5.91 10.05 -1.67
Mitotic Spindle -1.99 -1.49 -5.59 -2.61 -5.22 -5.14 -6.22 -9.59 -7.02 -6.65 -5.28 -6.36 -11.12 -7.54 -8.52
None 4.07 1.71 1.17 -6.63 -1.04 -1.14 -9.63 -7.01 -4.37 1.91 2.49 -2.21 -1.81 -6.22 0.43
Nuclear Periphery 2.47 -4.05 -3.54 -15.10 0.72 1.38 -15.18 -13.72 -10.55 1.00 2.51 -11.20 -9.53 -14.21 1.78
Nuclear Periphery Foci -1.06 -11.82 -14.23 -10.21 -5.97 0.72 -0.16 -3.53 -8.96 -7.06 0.48 -4.36 -9.19 -15.12 -7.83
Nucleolus 2.13 5.76 5.42 4.26 -1.76 -2.92 7.13 5.88 4.41 -1.02 -1.33 8.14 7.33 5.11 -2.00
Nucleus 8.88 -35.67 -0.48 -10.26 38.29 11.42 -31.99 0.53 -11.04 32.78 14.07 -48.01 0.12 -14.68 49.68
Peroxisomes -7.19 8.71 8.53 10.27 -1.92 -8.21 10.60 11.65 14.32 -0.20 -10.06 13.98 14.11 17.51 -0.71
Vacuole -4.23 -4.69 -30.84 -26.18 -28.61 -8.27 -10.49 -32.18 -27.40 -24.88 -9.25 -11.17 -44.00 -37.85 -37.16
Vacuole Periphery -2.31 8.41 3.25 4.13 -1.88 -6.65 6.91 -1.12 3.90 -3.69 -6.50 11.39 0.69 5.63 -4.20
Standard name
Human Ortholog
Description Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (13%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mix14

Mix14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mix14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available