Standard name
Human Ortholog
Description Transcriptional activator involved in regulating lysine biosynthesis; involved in the regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.06 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0.05 0 0 0
Bud Site 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.16 0.12 0.05 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0.11 0 0.06
Golgi 0 0 0 0 0 0.07 0 0 0 0 0
Mitochondria 0.16 0.06 0.07 0 0.12 0.06 0 0 0.07 0 0
Nucleus 0.68 0.5 0.72 0.81 0.76 0.57 0.61 0.61 0.41 0.52 0.41
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0.31 0.06 0.05 0 0.11 0.07 0.09 0.14 0.1 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0.05 0 0
Vac/Vac Membrane 0 0.12 0.14 0.09 0.12 0.06 0.22 0.11 0.09 0.21 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0
Bud 0 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 2 16 6 6 1 1 0 0 0 0
Endoplasmic Reticulum 1 0 8 9 2 0 0 0 0 0 0
Endosome 0 0 6 2 4 2 1 0 2 1 3
Golgi 0 0 1 0 0 4 0 0 0 0 2
Mitochondria 3 1 20 4 17 3 0 0 1 0 2
Nucleus 13 8 222 171 109 34 28 10 7 23 25
Nuclear Periphery 0 0 6 5 3 0 0 0 0 0 1
Nucleolus 1 5 19 10 6 6 3 1 2 4 7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 5 1 0 2 0 0 1 1 0
Vac/Vac Membrane 0 2 42 19 18 3 10 1 1 9 14
Unique Cell Count 19 16 307 210 144 61 46 18 19 45 61
Labelled Cell Count 21 18 345 227 165 61 46 18 19 45 61


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 6.3 8.5 8.9 8.4 5.1
Std Deviation (1e-4) 3.2 1.2 1.8 1.6 1.8 0.6
Intensity Change (Log2)

0246051002460510rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF1800510
rpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.052 0.0008 0.0004 0.0873 0.0005 0.0015
Bud 0.0009 0.0162 0.0001 0.0005 0.0001 0.0005
Bud Neck 0.0014 0.0005 0.0003 0.002 0.0061 0.0064
Bud Periphery 0.0012 0.0051 0.0004 0.0009 0.0002 0.0029
Bud Site 0.0081 0.0091 0.0003 0.0021 0.0003 0.001
Cell Periphery 0.0004 0.0007 0.0002 0.0004 0.0001 0.0005
Cytoplasm 0.0008 0.0162 0.0005 0.0033 0.0017 0.0003
Cytoplasmic Foci 0.0261 0.0182 0 0.002 0.0002 0.0003
Eisosomes 0.0008 0.0001 0 0.0006 0.0001 0.0001
Endoplasmic Reticulum 0.0011 0.0005 0.0002 0.0315 0.0004 0.0006
Endosome 0.0158 0.0014 0.0005 0.0137 0.0011 0.0047
Golgi 0.0187 0.0002 0 0.0056 0.0002 0.0018
Lipid Particles 0.0341 0.0015 0.0001 0.0057 0.0006 0.0036
Mitochondria 0.0218 0.0059 0.0028 0.0046 0.0007 0.0219
None 0.0015 0.005 0.0004 0.002 0.0007 0.0003
Nuclear Periphery 0.0071 0.0113 0.0388 0.104 0.0854 0.0239
Nucleolus 0.085 0.1785 0.1298 0.1871 0.1997 0.2066
Nucleus 0.6411 0.7126 0.8031 0.3824 0.6188 0.706
Peroxisomes 0.018 0.0005 0.0001 0.0006 0.0001 0.0006
Punctate Nuclear 0.023 0.0088 0.0157 0.1594 0.0801 0.006
Vacuole 0.0248 0.0046 0.0028 0.0016 0.0011 0.0048
Vacuole Periphery 0.0161 0.0024 0.0034 0.0026 0.0017 0.0057

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.969 22.446 19.1234 33.4406 30.445 26.0246 31.8362 35.0321 36.4248 44.4727
Translational Efficiency 0.2009 0.2506 0.324 0.1904 0.2378 0.2776 0.1954 0.2008 0.2015 0.1833

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
158 91 1751 1184 174 84 456 237 332 175 2207 1421

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 693.42 857.00 909.23 842.26 732.40 845.74 835.13 924.96 713.85 851.60 893.92 856.05
Standard Deviation 85.87 118.97 149.23 141.18 103.63 141.67 127.86 114.12 97.56 130.48 148.14 140.46
Intensity Change Log 2 0.305566 0.390916 0.280536 0.207582 0.189369 0.336759 0.256066 0.290906 0.309690

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000431 0.002012 0.000781 0.002247 0.001196 0.001674 0.000965 0.002518 0.000832 0.001850 0.000819 0.002292
Bud Neck 0.003807 0.015444 0.016664 0.030994 0.004412 0.005750 0.011297 0.034444 0.004124 0.010791 0.015555 0.031569
Bud Site 0.001525 0.002831 0.001736 0.007544 0.002824 0.001976 0.006402 0.006051 0.002206 0.002421 0.002700 0.007295
Cell Periphery 0.000188 0.000125 0.000170 0.000251 0.000211 0.000119 0.000245 0.000157 0.000200 0.000122 0.000186 0.000235
Cytoplasm 0.033822 0.006386 0.023954 0.015764 0.020268 0.004098 0.026665 0.005146 0.026719 0.005288 0.024514 0.013993
Cytoplasmic Foci 0.017581 0.007205 0.000834 0.005393 0.006202 0.010252 0.005388 0.001047 0.011617 0.008667 0.001775 0.004668
Eisosomes 0.000066 0.000085 0.000036 0.000054 0.000058 0.000099 0.000054 0.000053 0.000062 0.000092 0.000040 0.000054
Endoplasmic Reticulum 0.003456 0.011265 0.003884 0.007922 0.005285 0.009578 0.005037 0.009714 0.004415 0.010455 0.004122 0.008221
Endosome 0.001024 0.004449 0.000496 0.006127 0.000901 0.010042 0.005374 0.000988 0.000959 0.007134 0.001504 0.005270
Golgi 0.000661 0.001507 0.000185 0.003458 0.000444 0.004246 0.001487 0.001464 0.000547 0.002822 0.000454 0.003125
Lipid Particles 0.004874 0.003847 0.000275 0.001966 0.001783 0.009875 0.001003 0.000746 0.003254 0.006740 0.000425 0.001763
Mitochondria 0.003127 0.016314 0.001277 0.007517 0.007318 0.010028 0.003288 0.003714 0.005324 0.013297 0.001692 0.006883
Mitotic Spindle 0.000281 0.013107 0.007591 0.042536 0.006985 0.009276 0.006630 0.060519 0.003795 0.011268 0.007392 0.045535
None 0.024281 0.006780 0.014141 0.012385 0.012574 0.006526 0.015102 0.007751 0.018145 0.006658 0.014339 0.011612
Nuclear Periphery 0.001385 0.002783 0.001038 0.004173 0.001038 0.001165 0.001634 0.002760 0.001203 0.002006 0.001161 0.003937
Nuclear Periphery Foci 0.003253 0.005483 0.000978 0.005890 0.004233 0.008777 0.001000 0.005010 0.003767 0.007064 0.000983 0.005743
Nucleolus 0.111608 0.117313 0.080528 0.192853 0.091685 0.105107 0.065834 0.216276 0.101167 0.111454 0.077492 0.196760
Nucleus 0.783492 0.773028 0.842160 0.640272 0.828754 0.783991 0.834027 0.635407 0.807213 0.778290 0.840480 0.639460
Peroxisomes 0.003501 0.004950 0.000356 0.002137 0.002429 0.004454 0.001361 0.001538 0.002939 0.004712 0.000564 0.002037
Vacuole 0.001249 0.004362 0.002741 0.007358 0.001135 0.010554 0.005931 0.003983 0.001189 0.007335 0.003400 0.006795
Vacuole Periphery 0.000389 0.000724 0.000176 0.003159 0.000264 0.002411 0.001276 0.000715 0.000324 0.001534 0.000403 0.002751

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.15 -6.98 -7.85 -0.39 -4.71 -1.36 -0.05 -3.38 -1.27 -4.23 -2.95 -2.00 -5.77 -0.72 -5.89
Bud Neck -2.78 -13.56 -14.52 -3.50 -5.68 -1.01 -4.71 -9.46 -4.82 -6.58 -2.70 -15.66 -17.51 -5.82 -7.86
Bud Site -1.51 -2.64 -6.22 -2.96 -5.22 0.33 -1.12 -2.10 -2.32 -1.22 -0.80 -1.65 -5.44 -4.54 -4.69
Cell Periphery 1.49 1.44 -0.74 -2.73 -3.55 1.13 -0.04 0.92 -0.56 2.26 1.70 1.12 -0.06 -3.25 -2.64
Cytoplasm 3.74 2.37 2.90 -2.17 1.44 2.18 -0.43 3.04 0.90 4.78 4.23 1.83 3.31 -2.03 2.95
Cytoplasmic Foci 1.74 2.99 2.44 0.99 -3.68 -0.71 0.93 2.24 2.75 2.43 0.96 3.45 2.86 2.27 -2.31
Eisosomes -0.87 3.20 1.92 2.32 -3.46 -2.26 0.40 0.02 2.37 -0.52 -2.36 3.29 1.76 3.49 -3.19
Endoplasmic Reticulum -3.42 -2.82 -5.68 1.36 -5.04 -2.46 -0.83 -3.20 0.23 -2.75 -4.16 -2.05 -5.78 1.53 -5.64
Endosome -1.29 1.20 -4.21 -0.18 -4.94 -2.03 -2.09 -0.48 2.00 2.02 -2.40 -1.17 -4.38 1.16 -3.41
Golgi -0.88 1.85 -2.28 -1.17 -3.04 -1.65 -1.39 -1.34 1.25 -0.03 -1.86 0.71 -2.69 0.27 -2.90
Lipid Particles 0.63 3.32 2.48 1.40 -2.42 -1.82 1.90 2.44 2.11 0.89 -1.40 4.06 2.67 2.36 -2.21
Mitochondria -1.30 2.54 -3.14 0.92 -5.42 -0.52 0.85 0.68 1.82 -0.78 -1.33 1.31 -0.35 1.29 -5.34
Mitotic Spindle -1.99 -7.89 -11.15 -2.90 -7.37 -0.53 -0.43 -4.95 -4.74 -4.97 -1.88 -3.25 -10.10 -5.67 -8.91
None 2.58 2.24 2.27 -1.00 0.14 0.62 -0.01 2.68 1.10 2.54 2.50 2.23 2.67 -0.27 0.96
Nuclear Periphery -2.02 -0.52 -3.78 -1.92 -4.33 -0.40 -1.08 -3.64 -3.31 -1.76 -2.09 -1.43 -4.93 -3.43 -4.57
Nuclear Periphery Foci -1.37 1.41 -1.26 1.05 -4.93 -0.85 1.30 -0.36 0.69 -1.73 -1.59 2.30 -1.03 1.28 -5.10
Nucleolus 0.41 0.67 -5.54 -5.71 -11.05 -0.21 1.51 -5.66 -4.71 -7.98 0.08 0.54 -8.57 -7.62 -13.43
Nucleus 0.27 -1.40 6.93 4.92 16.78 1.46 -0.17 7.84 4.29 9.59 1.31 -0.62 11.12 6.55 19.28
Peroxisomes -0.34 3.56 1.92 1.62 -3.44 -1.79 1.52 0.73 2.23 -1.41 -1.53 4.22 1.85 2.70 -3.55
Vacuole -2.14 -4.70 -6.13 -1.83 -2.49 -2.39 -3.38 -4.51 1.05 -0.02 -2.99 -5.89 -7.12 0.09 -2.28
Vacuole Periphery -1.40 1.12 -3.32 -2.65 -3.69 -2.19 -2.13 -4.92 1.71 0.91 -2.48 -0.79 -3.63 -0.78 -3.44
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Componentsactinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Transcriptional activator involved in regulating lysine biosynthesis; involved in the regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Lys14

Lys14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lys14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available