Standard name
Human Ortholog
Description Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0 0 0 0 0 0 0.07 0.06 0.08 0.06
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.41 0.46 0.74 0.88 0.68 0.81 0.8 0.79 0.7 0.67 0.65 0.64 0.65 0.69 0.11 0.15 0.13 0.05 0.05 0.12 0.18 0.13 0.21
Endoplasmic Reticulum 0 0.13 0.07 0 0 0 0 0 0 0 0 0.16 0.17 0 0 0.06 0.05 0.05 0.09 0.14 0.18 0.19 0.13
Endosome 0.08 0.07 0.06 0 0.07 0 0 0 0 0 0 0.06 0 0.12 0.05 0.06 0.06 0.05 0 0.06 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.14 0.22 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.11 0.15 0.31 0.41 0.49 0.54 0 0 0 0.05 0 0 0 0 0.07 0.08 0.06 0.07
Nucleus 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0.21 0.09 0.1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.5 0.38 0.12 0 0.3 0.17 0.1 0.05 0.05 0 0 0.06 0.06 0.15 0.55 0.62 0.62 0.76 0.7 0.44 0.32 0.31 0.34
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 4 1 6 0 0 1 0 0 0 0 56 58 37 3 2 2 2 8 24 20 45 34
Bud 2 0 3 8 0 3 13 12 19 16 15 25 25 8 0 1 0 13 9 10 15 37 32
Bud Neck 0 0 1 0 0 2 1 1 3 0 2 2 2 1 0 0 0 3 3 10 17 23 28
Bud Site 0 0 0 0 0 0 0 0 1 0 1 1 1 1 0 0 0
Cell Periphery 2 3 3 4 0 2 0 0 0 1 1 15 15 2 1 0 0 0 0 1 8 25 19
Cytoplasm 155 139 238 175 249 293 468 318 338 257 279 507 507 579 14 25 10 19 15 43 58 77 128
Endoplasmic Reticulum 16 38 21 1 0 0 0 0 1 1 2 126 130 13 4 10 4 20 30 50 59 111 80
Endosome 32 20 19 6 27 9 15 8 6 4 2 46 34 101 6 10 5 22 15 19 9 20 13
Golgi 2 2 1 1 2 0 0 0 0 0 0 1 3 6 17 24 17 2 3 2 3 6 1
Mitochondria 14 8 3 2 6 38 90 127 199 187 233 0 0 1 6 7 3 6 5 25 25 34 40
Nucleus 31 9 4 1 5 10 5 1 2 0 1 5 5 1 28 15 8 1 1 0 0 1 1
Nuclear Periphery 0 1 1 0 0 0 4 0 0 1 0 0 1 0 0 1 0 2 0 0 0 0 2
Nucleolus 3 1 0 0 2 0 2 1 2 1 0 1 1 1 1 6 1 0 0 0 0 0 0
Peroxisomes 2 1 5 2 2 3 2 0 3 0 1 0 0 3 1 0 0 1 0 1 0 3 0
SpindlePole 11 5 12 5 8 6 13 2 20 11 11 24 26 33 6 1 1 6 6 6 5 16 12
Vac/Vac Membrane 190 114 39 5 109 62 61 20 23 7 9 51 49 124 73 107 48 322 244 156 105 186 208
Unique Cell Count 379 302 323 200 365 361 582 404 481 383 429 790 783 843 133 172 78 426 350 357 334 599 615
Labelled Cell Count 463 345 351 216 410 428 675 490 617 486 557 860 857 911 160 209 99 426 350 357 334 599 615


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 8.7 5.5 5.6 5.8 4.2 3.7 3.3 2.9 3.0 2.8 8.9 8.9 8.7 14.8 17.4 20.4 7.2 7.6 7.0
Std Deviation (1e-4) 2.8 3.6 1.9 2.2 3.1 2.0 1.2 1.0 1.0 0.9 0.9 3.0 3.0 2.8 5.1 6.5 8.6 2.8 2.7 2.4
Intensity Change (Log2) 0.02 0.07 -0.4 -0.58 -0.74 -0.92 -0.89 -0.96 0.68 0.68 0.65 1.41 1.65 1.88 0.38 0.45 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 4.7 4.8 3.5 0 0 0
Bud 0 0 0 0 0 0 0 0 2.2 2.2 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 1.2 1.2 0 0 0 0
Cytoplasm 3.8 -1.6 2.3 2.3 1.6 -1.1 -1.9 -2.5 -3.1 -2.9 -1.7 -12.3 -12.6 -9.9
Endoplasmic Reticulum -3.3 -4.9 0 0 0 0 0 0 4.2 4.4 -4.5 -1.5 -0.3 0
Endosome -1.5 0.8 0 0 0 0 0 0 0 -1.1 3.1 -0.6 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 6.2 6.6 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 1.4 0 0 0 0 0 0 0 0 7.5 4.1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -0.8 -1.2 0 0 0 0 0 0 -0.6 -0.3 0.2 0.4 0 0
Vacuole -3.8 5.7 1.9 -0.7 -3.5 -3.8 -5.5 -5.5 -3.1 -3.3 1.2 9.7 11.6 9.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 20.3491 25.9075 24.9793 23.7005 20.9054 24.4547 19.4576 26.4996 26.0136 27.3445 15.9235 25.9819 23.2414 32.4088 27.9739 28.4442 25.6524 30.0906
Actin 0.0068 0.0011 0.0009 0.0001 0.0142 0.0001 0.0159 0.0002 0.0152 0 0.0019 0.0001 0.0072 0.0001 0.0048 0 0.0006 0.0006
Bud 0.001 0.0028 0.0006 0.0006 0.0007 0.0004 0.0017 0.0004 0.0007 0.0004 0.0004 0.0004 0.0005 0.0002 0.0005 0.0001 0.0002 0.0002
Bud Neck 0.0011 0.0019 0.0011 0.0009 0.0015 0.005 0.0012 0.0011 0.0017 0.001 0.0007 0.0016 0.0012 0.0007 0.0017 0.0004 0.0006 0.002
Bud Periphery 0.0026 0.0026 0.0019 0.0041 0.0027 0.0027 0.0062 0.0015 0.0021 0.0019 0.0019 0.0019 0.0023 0.001 0.0017 0.0008 0.0014 0.0014
Bud Site 0.0019 0.0111 0.0018 0.0004 0.001 0.0004 0.0018 0.0048 0.0054 0.0008 0.0015 0.0007 0.0012 0.0008 0.0016 0.0003 0.0004 0.0003
Cell Periphery 0.1127 0.1344 0.1083 0.0981 0.0864 0.1472 0.068 0.1331 0.1041 0.0918 0.1669 0.0957 0.1124 0.1693 0.1145 0.1676 0.1398 0.1224
Cytoplasm 0.048 0.0325 0.0549 0.0075 0.0118 0.0166 0.019 0.0198 0.0239 0.0039 0.0118 0.0206 0.0092 0.0048 0.0256 0.0024 0.0035 0.0067
Cytoplasmic Foci 0.0154 0.0042 0.0057 0.0004 0.0071 0.0017 0.0294 0.002 0.004 0.0057 0.0045 0.0032 0.0097 0.0032 0.006 0.0005 0.0142 0.0082
Eisosomes 0.0002 0 0.0001 0 0.0004 0 0.0002 0 0.0005 0 0.0002 0 0.0003 0 0 0 0 0
Endoplasmic Reticulum 0.0407 0.0232 0.0255 0.0227 0.0071 0.0388 0.0286 0.0292 0.0446 0.0074 0.0155 0.0285 0.016 0.0336 0.0412 0.0082 0.0041 0.0149
Endosome 0.0545 0.0213 0.0352 0.0163 0.0318 0.0284 0.1095 0.02 0.0453 0.0677 0.0198 0.046 0.0603 0.0234 0.0457 0.0147 0.0417 0.024
Golgi 0.0043 0.0022 0.0027 0.0003 0.0066 0.0013 0.0122 0.0018 0.0079 0.0015 0.0017 0.0045 0.0069 0.0028 0.0094 0.0004 0.0176 0.003
Lipid Particles 0.0559 0.052 0.0443 0.0054 0.0233 0.0368 0.0594 0.0365 0.0536 0.0163 0.0226 0.0542 0.0866 0.0754 0.0733 0.0273 0.0563 0.0498
Mitochondria 0.009 0.0004 0.0105 0.0001 0.0317 0.0004 0.0262 0.0015 0.0018 0.0001 0.0827 0.0004 0.0125 0.0005 0.0014 0.0001 0.0007 0.0004
None 0.0031 0.0001 0.001 0.0001 0.0031 0.0001 0.0085 0.0005 0.0007 0 0.0016 0.0001 0.0044 0.0001 0.0002 0 0.0003 0.0001
Nuclear Periphery 0.0117 0.0048 0.0055 0.0041 0.0081 0.0063 0.0412 0.0049 0.0081 0.0034 0.0079 0.0049 0.0096 0.0048 0.0056 0.0022 0.0033 0.0078
Nucleolus 0.0062 0.0055 0.0037 0.0255 0.0046 0.0012 0.002 0.0076 0.0019 0.0038 0.0632 0.0019 0.0052 0.0028 0.0032 0.0029 0.0012 0.0011
Nucleus 0.0088 0.0051 0.005 0.0124 0.005 0.0029 0.0121 0.0039 0.0032 0.003 0.0241 0.0023 0.0058 0.0021 0.0025 0.0016 0.0017 0.0021
Peroxisomes 0.0065 0.0001 0.0002 0 0.0015 0 0.0015 0.0001 0.001 0.0001 0.0017 0.0001 0.0093 0.0001 0.0037 0 0.0006 0.0047
Punctate Nuclear 0.0027 0.0001 0.0004 0 0.0014 0.0001 0.007 0.0001 0.0003 0.0001 0.0106 0.0001 0.0007 0.0001 0.0003 0 0.001 0.0002
Vacuole 0.5795 0.6763 0.6596 0.7738 0.7182 0.6891 0.5096 0.6967 0.6338 0.7408 0.5396 0.7018 0.5832 0.637 0.6113 0.739 0.6848 0.6999
Vacuole Periphery 0.0273 0.0182 0.031 0.0271 0.0318 0.0204 0.0389 0.0343 0.04 0.0502 0.0191 0.031 0.0554 0.0373 0.0459 0.0314 0.026 0.0501

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.4984 47.8243 92.3487 118.5477 75.6687 51.0384 75.4175 91.0482 95.0529 91.5652
Translational Efficiency 1.1952 1.3622 1.039 0.837 0.9779 1.4113 0.9053 0.6453 0.8127 0.7054

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
106 1010 1704 1392 1791 1796 1722 1728 1897 2806 3426 3120

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1261.28 1301.48 1966.62 1709.42 737.54 1375.05 1861.93 1862.76 766.81 1348.57 1914.00 1794.35
Standard Deviation 251.62 383.04 336.58 390.34 213.98 380.16 280.59 334.88 247.46 382.83 314.10 368.64
Intensity Change Log 2 0.045265 0.640830 0.438618 0.898691 1.336006 1.336649 0.421215 0.937650 0.837656

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000820 0.002050 0.001088 0.000909 0.001899 0.000847 0.001164 0.000660 0.001838 0.001280 0.001126 0.000771
Bud Neck 0.006828 0.021613 0.005287 0.011707 0.063114 0.020444 0.008063 0.009917 0.059969 0.020865 0.006682 0.010716
Bud Site 0.037889 0.085349 0.006777 0.038445 0.058471 0.047628 0.009722 0.021021 0.057321 0.061206 0.008258 0.028794
Cell Periphery 0.003049 0.231987* 0.217459* 0.185404* 0.025758 0.368271* 0.270141* 0.217228* 0.024489 0.319216* 0.243938* 0.203030*
Cytoplasm 0.058004 0.154619* 0.008359 0.048803 0.370019* 0.059659 0.012242 0.015307 0.352585* 0.093839 0.010311 0.030252
Cytoplasmic Foci 0.031530 0.017401 0.001030 0.005584 0.112057 0.004625 0.000900 0.001923 0.107557 0.009224 0.000965 0.003557
Eisosomes 0.000033 0.000307 0.000550 0.000207 0.001842 0.000357 0.000541 0.000140 0.001741 0.000339 0.000546 0.000170
Endoplasmic Reticulum 0.018526 0.071459 0.246581* 0.061011 0.016325 0.101561* 0.220118* 0.084884 0.016448 0.090726 0.233280* 0.074233
Endosome 0.062573 0.017072 0.014470 0.015976 0.028542 0.011146 0.011977 0.013604 0.030443 0.013279 0.013217 0.014662
Golgi 0.022426 0.005882 0.006148 0.010611 0.004393 0.002115 0.003479 0.006084 0.005401 0.003471 0.004806 0.008104
Lipid Particles 0.005846 0.021710 0.223756* 0.104730* 0.013726 0.010988 0.145702* 0.078098 0.013285 0.014847 0.184524* 0.089980
Mitochondria 0.011501 0.000670 0.000585 0.001118 0.004288 0.001398 0.000323 0.000547 0.004691 0.001136 0.000453 0.000802
Mitotic Spindle 0.003662 0.000149 0.000183 0.000283 0.001328 0.000247 0.000148 0.000474 0.001458 0.000211 0.000166 0.000389
None 0.000021 0.001567 0.000081 0.000151 0.003541 0.000850 0.000026 0.000040 0.003345 0.001108 0.000053 0.000090
Nuclear Periphery 0.000185 0.000247 0.001941 0.000703 0.000248 0.000391 0.001936 0.000848 0.000245 0.000339 0.001939 0.000783
Nuclear Periphery Foci 0.001387 0.002638 0.003665 0.004212 0.000677 0.001387 0.003031 0.002470 0.000716 0.001837 0.003346 0.003247
Nucleolus 0.000134 0.000601 0.000079 0.000280 0.001235 0.000207 0.000086 0.000232 0.001173 0.000349 0.000082 0.000253
Nucleus 0.001368 0.002064 0.000278 0.000913 0.055076 0.001273 0.000276 0.000420 0.052075 0.001558 0.000277 0.000640
Peroxisomes 0.002167 0.001960 0.004893 0.001683 0.004980 0.001493 0.004621 0.001999 0.004822 0.001661 0.004756 0.001858
Vacuole 0.692986* 0.352481* 0.253610* 0.488344* 0.230357* 0.360681* 0.303316* 0.530685* 0.256208* 0.357729* 0.278594* 0.511795*
Vacuole Periphery 0.039065 0.008174 0.003179 0.018927 0.002125 0.004431 0.002185 0.013418 0.004189 0.005778 0.002680 0.015876

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.44 -0.84 -0.47 2.55 0.65 4.13 2.38 4.87 1.35 3.14 1.99 2.52 4.42 2.74 2.40
Bud Neck -6.10 0.87 -3.05 4.62 -6.91 13.55 19.32 18.57 6.36 -1.82 13.53 20.00 18.29 7.75 -5.92
Bud Site -4.10 2.79 -0.27 7.78 -11.29 2.33 13.79 9.48 6.96 -4.97 -1.06 15.05 7.60 9.75 -11.59
Cell Periphery -28.67* -43.48* -33.25* 5.11 4.71 -50.04* -43.97* -35.24* 18.73 7.58 -52.89 -53.77 -42.52 18.68 8.33
Cytoplasm -4.78 4.29 1.52 13.04 -11.66 42.71* 55.80 55.04 11.45 -1.93 36.19 54.89 49.96 15.76 -11.00
Cytoplasmic Foci 2.20 4.78 4.05 5.78 -6.78 26.96 28.28 28.01 3.50 -2.70 25.66 28.46 27.66 6.23 -7.15
Eisosomes -9.69 -7.10 -4.58 2.16 4.20 9.81 8.50 11.44 6.39 10.16 9.88 8.18 11.12 5.86 8.42
Endoplasmic Reticulum -5.81 -22.11 -4.86 1.91 25.69 -20.20* -31.19 -17.98 3.22 18.66 -22.16 -45.90 -19.72 4.31 31.00
Endosome 4.96 5.33 5.12 0.56 -1.35 10.72 10.48 9.43 -2.34 -1.63 10.59 11.38 10.12 -1.54 -1.96
Golgi 1.84 1.81 1.22 -3.84 -4.20 4.56 1.76 -1.72 -4.03 -2.61 2.81 0.79 -3.24 -6.08 -4.54
Lipid Particles -5.33 -31.17* -18.03 -15.37 14.34 1.67 -22.80 -16.38 -16.68 9.92 -1.09 -37.91 -23.81 -22.93 17.60
Mitochondria 1.49 1.50 1.42 -0.88 -1.10 4.37 7.25 6.66 2.15 -1.53 5.16 6.60 5.73 1.02 -1.51
Mitotic Spindle 1.75 1.73 1.68 -4.67 -3.60 1.62 1.79 1.22 -0.96 -1.51 1.98 2.06 1.67 -1.31 -1.87
None -2.43 -3.65 -3.74 2.21 -1.92 3.57 4.95 4.93 2.94 -1.44 3.11 4.90 4.85 3.51 -2.07
Nuclear Periphery -0.91 -19.27 -7.32 -9.67 14.52 -2.77 -18.94 -10.23 -8.36 12.05 -2.17 -25.70 -11.04 -11.62 18.58
Nuclear Periphery Foci -3.92 -8.24 -8.03 -5.24 -2.11 -7.43 -19.66 -16.82 -8.97 3.96 -11.30 -27.01 -18.60 -9.53 0.48
Nucleolus -1.58 2.12 -5.50 1.10 -13.86 2.55 2.83 2.49 -1.69 -12.48 2.09 2.84 2.42 0.92 -18.72
Nucleus -1.03 4.20 1.92 4.12 -6.12 23.16 23.61 23.55 6.53 -4.66 22.84 23.45 23.27 6.78 -7.25
Peroxisomes 0.13 -3.58 0.59 1.16 6.16 7.81 1.14 6.71 -2.00 5.47 7.64 0.76 7.21 -0.99 8.18
Vacuole 11.46 15.14 6.93 -12.19 -25.40 -15.81 -9.09 -34.81 -20.00 -27.10 -12.93 -3.22 -32.42 -22.86 -37.44
Vacuole Periphery 3.10 3.68 1.86 -6.97 -11.42 -4.94 -0.30 -11.88 -8.59 -11.56 -2.74 2.33 -12.37 -11.31 -16.13
Standard name
Human Ortholog
Description Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (18%), ER (6%), mixed (35%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Bap3

Bap3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bap3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available