Standard name
Human Ortholog
Description Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human homolog TPI1 causes a rare autosomal disease; human TPI1 can complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 1.0 0.99 0.99 0.98 0.93 0.93 0.96 0.88 0.94 0.91 1.0 0.99 1.0 0.99 0.97 0.98 0.98 0.97 0.96 0.96 0.95 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.05 0 0.1 0 0 0 0 0 0 0.07 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 1 0 0 0 0 1 1 1 0 0 0 0 1 0 0 2 2 0 0 1 0 6 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 0 1 4 2 6 9 7 4 8 10 0 0 3 0 2 1 0 0 0 0 0 0
Cytoplasm 147 262 151 137 119 213 194 291 142 127 165 204 206 281 312 241 262 205 137 250 135 208 261 296
Endoplasmic Reticulum 0 1 0 1 0 3 8 15 2 14 3 9 0 0 0 6 20 14 1 0 0 0 0 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 1 4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 3 1 1 0 0 0 1 0 0
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 2 3 0 1 0 1 1 9
Unique Cell Count 147 265 151 138 120 217 208 312 148 144 176 223 206 284 312 244 270 210 140 257 142 217 277 340
Labelled Cell Count 148 267 151 139 123 218 209 316 152 145 177 224 206 284 317 250 293 226 140 257 142 217 277 340


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 198.8 161.0 176.7 218.5 213.6 177.6 159.6 149.7 135.6 141.5 133.4 132.0 204.1 202.5 202.6 101.6 155.8 144.9 196.4 197.8 180.2
Std Deviation (1e-4) 35.1 38.3 46.5 56.6 38.6 46.5 39.0 32.5 33.9 28.2 34.3 32.4 62.8 59.2 52.8 39.7 52.2 56.7 57.2 61.9 64.7
Intensity Change (Log2) – – – 0.31 0.27 0.01 -0.15 -0.24 -0.38 -0.32 -0.41 -0.42 0.21 0.2 0.2 -0.8 -0.18 -0.29 0.15 0.16 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.0 -1.1 -1.7 -3.3 -3.3 -2.5 -4.3 -3.1 -3.7 0 -1.3 0 -1.4 -2.1 -1.9
Endoplasmic Reticulum 0 0 0 0 0 0 3.9 0 0 0 0 0 0 3.4 3.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 248.9185 356.7186 324.9163 260.5118 311.7177 291.0993 254.0984 360.9434 320.0826 310.7015 212.0604 278.4056 292.0722 376.195 325.1528 320.9733 329.0699 352.9407
Actin 0.0235 0.0001 0.0002 0.0296 0.0011 0.0141 0.0151 0.0002 0.0031 0.0001 0.002 0.001 0.0072 0.0001 0.0045 0.0047 0.0001 0.0001
Bud 0.0011 0.0001 0.0006 0.0042 0.005 0.0002 0.0041 0.0001 0.0006 0 0.0036 0.0006 0.0005 0.0001 0.001 0 0.0001 0
Bud Neck 0.0007 0.0001 0.0002 0.0005 0.0005 0.0006 0.0029 0.0001 0.0008 0.0002 0.0005 0.0005 0.0005 0.0001 0.0002 0.0002 0.0002 0.0003
Bud Periphery 0.0008 0 0.0003 0.0019 0.0012 0.0001 0.0016 0 0.0004 0 0.0016 0.0001 0.0007 0 0.0005 0 0 0
Bud Site 0.0024 0 0.0001 0.0007 0.0002 0.0001 0.0065 0.0003 0.0024 0 0.0007 0 0.0014 0 0.0003 0.0001 0 0
Cell Periphery 0.0006 0 0.0002 0.0009 0.0004 0.0001 0.001 0.0004 0.0003 0.0001 0.0028 0 0.0002 0 0.0005 0 0 0
Cytoplasm 0.8199 0.9888 0.9551 0.839 0.941 0.8517 0.8385 0.9839 0.9454 0.9495 0.7489 0.983 0.9148 0.992 0.9405 0.9361 0.9773 0.9941
Cytoplasmic Foci 0.027 0 0.0077 0.0014 0.0039 0.0303 0.0257 0.0023 0.0079 0.0195 0.0582 0.0003 0.006 0.0001 0.0102 0.0032 0.0001 0.0002
Eisosomes 0.0006 0 0.0001 0.0007 0.0001 0.0001 0.0009 0.0004 0.0002 0.0003 0.0009 0 0.0002 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.004 0 0.0001 0.0003 0.0008 0.0023 0.0006 0.0001 0.0004 0 0.0006 0.0002 0.0016 0 0.0001 0.0001 0 0
Endosome 0.0231 0 0 0.0018 0.0002 0.0235 0.0037 0 0.0041 0 0.0145 0.0001 0.0106 0 0.0007 0.0017 0 0
Golgi 0.0086 0 0 0.004 0 0.0099 0.0029 0 0.0016 0 0.0121 0 0.0023 0 0.0012 0.0033 0 0
Lipid Particles 0.0097 0 0.0008 0.0008 0.0001 0.0072 0.0074 0.0001 0.0016 0.0019 0.0271 0 0.0053 0 0.0042 0.0124 0 0
Mitochondria 0.0155 0.0001 0.0099 0.0768 0.0085 0.003 0.0201 0.0025 0.0113 0.0054 0.0321 0.0004 0.0129 0.0002 0.0113 0.0013 0.0001 0.0001
None 0.0113 0.0001 0.0076 0.0088 0.0145 0.0343 0.0197 0.002 0.0056 0.0061 0.021 0.0004 0.0034 0.0004 0.006 0.0003 0.0002 0.0003
Nuclear Periphery 0.0114 0.0008 0.0008 0.0034 0.0026 0.0065 0.005 0.0009 0.001 0.002 0.0046 0.0021 0.0058 0.0008 0.0015 0.0017 0.0028 0.0007
Nucleolus 0.0099 0 0.0084 0.0047 0.0023 0.0023 0.0108 0.0025 0.0037 0.0052 0.0146 0 0.0004 0.0002 0.0032 0 0 0
Nucleus 0.0135 0.0097 0.0054 0.0149 0.0129 0.0069 0.0195 0.0035 0.0039 0.0081 0.0147 0.0106 0.0209 0.0057 0.0117 0.0161 0.0187 0.0041
Peroxisomes 0.0084 0 0.0005 0.0014 0.0004 0.0017 0.0038 0.0001 0.0014 0.0006 0.0046 0 0.0019 0 0.0016 0.0182 0 0
Punctate Nuclear 0.0029 0 0.0002 0.0002 0.0007 0.0035 0.0068 0.0001 0.0002 0.0002 0.001 0.0001 0.0019 0.0001 0.0004 0.0003 0.0001 0.0001
Vacuole 0.0034 0.0001 0.0008 0.0022 0.0024 0.001 0.0021 0.0003 0.0026 0.0003 0.0162 0.0003 0.001 0.0001 0.0004 0.0001 0.0001 0
Vacuole Periphery 0.0018 0 0.001 0.002 0.0013 0.0007 0.0012 0.0002 0.0013 0.0005 0.0176 0.0002 0.0006 0 0.0002 0.0001 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2964.5686 3512.3971 4528.7784 3212.5393 3514.2881 3518.0149 3918.8689 3836.0097 4903.609 3452.3696
Translational Efficiency 1.3668 1.3763 1.1856 1.9328 1.5559 1.2596 1.5292 1.8198 1.3756 1.6605

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
122 343 2225 1981 1945 1944 1328 1233 2067 2287 3553 3214

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 8629.18 12911.18 18538.63 17068.13 11951.11 14426.27 18721.43 16388.87 11755.04 14199.04 18606.95 16807.54
Standard Deviation 1617.78 4239.58 3341.77 4294.07 2409.13 2966.16 3982.30 4708.52 2495.74 3235.26 3595.65 4469.84
Intensity Change Log 2 — 0.581325 1.103239 0.984010 — 0.271554 0.647546 0.455572 — 0.409615 0.856367 0.701045

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000176 0.000094 0.000036 0.000142 0.000031 0.000035 0.000053 0.000128 0.000039 0.000044 0.000043 0.000137
Bud Neck 0.001966 0.001072 0.000335 0.001273 0.000236 0.000243 0.001046 0.000957 0.000338 0.000368 0.000601 0.001152
Bud Site 0.008078 0.003198 0.000835 0.005417 0.000428 0.001211 0.001162 0.003634 0.000880 0.001509 0.000957 0.004733
Cell Periphery 0.007102 0.009074 0.002160 0.003002 0.001877 0.000927 0.002378 0.001542 0.002185 0.002149 0.002241 0.002442
Cytoplasm 0.796527* 0.798475* 0.886319* 0.840295* 0.873117* 0.861694* 0.773300* 0.849009* 0.868597* 0.852212* 0.844076* 0.843638*
Cytoplasmic Foci 0.025768 0.015229 0.002777 0.006079 0.004616 0.003761 0.003835 0.004036 0.005864 0.005481 0.003172 0.005295
Eisosomes 0.000002 0.000002 0.000001 0.000003 0.000001 0.000000 0.000001 0.000002 0.000001 0.000001 0.000001 0.000003
Endoplasmic Reticulum 0.000137 0.000032 0.000016 0.000029 0.000017 0.000010 0.000021 0.000035 0.000024 0.000013 0.000018 0.000031
Endosome 0.000250 0.000236 0.000034 0.000136 0.000034 0.000044 0.000059 0.000221 0.000046 0.000073 0.000043 0.000169
Golgi 0.010927 0.002351 0.000914 0.000384 0.002564 0.000623 0.000534 0.000426 0.003057 0.000882 0.000772 0.000400
Lipid Particles 0.005376 0.005127 0.001196 0.002587 0.000960 0.000624 0.001486 0.001703 0.001220 0.001299 0.001305 0.002248
Mitochondria 0.000330 0.000125 0.000085 0.001070 0.000128 0.000097 0.000144 0.000232 0.000139 0.000101 0.000107 0.000749
Mitotic Spindle 0.012756 0.000550 0.000129 0.001456 0.000367 0.000399 0.000275 0.000462 0.001098 0.000421 0.000183 0.001075
None 0.005027 0.023448 0.006188 0.009945 0.011330 0.015504 0.005852 0.014179 0.010958 0.016695 0.006063 0.011570
Nuclear Periphery 0.000618 0.000337 0.000170 0.000152 0.000178 0.000088 0.000219 0.000129 0.000204 0.000125 0.000188 0.000143
Nuclear Periphery Foci 0.004719 0.002795 0.001158 0.000879 0.001321 0.000816 0.000765 0.000990 0.001522 0.001113 0.001011 0.000921
Nucleolus 0.000201 0.000116 0.000047 0.000175 0.000041 0.000044 0.000131 0.000133 0.000051 0.000055 0.000078 0.000159
Nucleus 0.093211 0.115330 0.085081 0.081426 0.096055 0.108459 0.182506* 0.102590 0.095887 0.109489 0.121495 0.089545
Peroxisomes 0.000021 0.000016 0.000009 0.000073 0.000002 0.000013 0.000009 0.000025 0.000003 0.000014 0.000009 0.000055
Vacuole 0.019548 0.020351 0.011988 0.045036 0.004223 0.004672 0.025707 0.019288 0.005128 0.007024 0.017116 0.035158
Vacuole Periphery 0.007258 0.002042 0.000524 0.000439 0.002476 0.000736 0.000517 0.000278 0.002758 0.000932 0.000521 0.000377

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.85 3.53 0.28 -3.04 -7.42 -1.79 -6.64 -5.60 -5.15 -2.49 -1.92 -4.96 -9.27 -8.50 -7.46
Bud Neck 1.09 2.06 0.66 -1.59 -9.88 -0.82 -11.28 -4.54 -4.41 3.64 -1.07 -9.38 -9.19 -9.19 -1.58
Bud Site 1.70 2.62 0.76 -2.63 -7.01 -3.03 -3.90 -6.18 -4.19 -2.79 -2.33 -2.07 -8.42 -6.53 -7.46
Cell Periphery -0.64 3.01 3.03 2.83 0.05 3.86 -0.78 1.23 -3.62 1.78 0.22 -0.70 0.12 -0.14 0.90
Cytoplasm 0.33 -3.88 -0.52 -1.63 12.83 3.86 24.23 9.85 6.97 -14.43 5.21 13.06 13.64 8.89 0.51
Cytoplasmic Foci 1.25 4.21 3.46 5.18 -3.79 0.13 -3.61 -3.24 -3.48 0.63 -1.18 0.81 -1.91 -0.95 -2.94
Eisosomes -0.22 2.21 -0.67 -0.68 -6.88 0.38 -7.32 -4.56 -4.78 -0.01 -1.24 -5.72 -8.29 -8.03 -4.93
Endoplasmic Reticulum 2.66 3.06 2.85 0.74 -2.21 4.10 -5.50 -1.60 -2.56 -0.11 3.61 0.84 -0.64 -3.65 -1.43
Endosome 0.24 3.76 1.53 0.45 -4.76 -0.27 -4.00 -1.45 -1.35 -0.69 -0.66 -0.74 -2.70 -1.92 -2.56
Golgi 3.79 4.42 4.70 5.05 5.02 7.31 7.49 8.49 3.05 2.96 7.57 7.79 9.65 5.65 5.76
Lipid Particles -0.29 2.18 0.73 1.23 -5.49 1.03 -2.96 -3.05 -3.68 -1.01 -0.80 -2.58 -6.08 -5.37 -4.65
Mitochondria 1.62 1.78 -0.49 -1.28 -1.35 2.04 -3.73 -1.84 -2.45 -0.64 2.55 1.49 -1.31 -1.49 -1.39
Mitotic Spindle 1.72 1.77 1.55 -2.09 -2.95 -0.50 -0.13 -0.01 0.36 0.07 1.34 1.98 -0.16 -1.89 -2.81
None -3.81 -1.84 -1.63 3.24 -0.81 -3.45 10.45 0.39 2.37 -4.00 -4.34 10.03 2.65 5.74 -3.28
Nuclear Periphery 1.12 1.61 1.65 2.70 0.58 4.47 -7.03 -1.05 -5.90 6.33 2.46 -2.64 0.07 -4.20 4.37
Nuclear Periphery Foci 0.93 2.09 1.97 3.17 -0.83 1.25 0.35 -1.14 -2.55 -1.61 0.76 0.82 -0.26 -1.28 -1.46
Nucleolus 0.99 1.60 0.71 -1.10 -7.61 -1.41 -11.21 -2.60 -2.47 1.39 -0.43 -5.34 -5.34 -5.40 -2.76
Nucleus -1.59 0.23 0.01 3.15 -0.77 -4.37 -20.19 -4.79 -0.87 16.10 -4.90 -11.76 -2.92 2.52 10.39
Peroxisomes 0.63 2.45 -3.49 -3.94 -5.11 -3.81 -4.13 -3.62 -2.11 -2.31 -4.03 -5.16 -6.48 -4.89 -5.61
Vacuole -1.10 0.81 -6.00 -6.98 -17.20 -2.33 -15.50 -13.97 -13.27 4.25 -4.36 -16.53 -24.88 -22.10 -11.16
Vacuole Periphery 1.93 2.46 2.52 1.96 2.14 2.53 3.61 4.32 0.89 4.87 2.85 4.25 4.74 1.21 4.23
Standard name
Human Ortholog
Description Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human homolog TPI1 causes a rare autosomal disease; human TPI1 can complement yeast null mutant
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Tpi1

Tpi1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tpi1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available