Standard name | |
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Human Ortholog | |
Description | Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress; co-expression of human CDC7 and DBF4 complements single cdc7 or dbf4 null mutations or the cdc7 dbf4 double null mutation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 2 | 0 | 2 | 0 | 2 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 5 | 0 | 7 | 6 |
Bud | 0 | 0 | 0 | 0 | 2 | 1 | 6 | 1 | 7 | – | 9 | 7 | 1 | 0 | 0 | 0 | 0 | 0 | 5 | 5 | 3 | 4 | 4 | 10 |
Bud Neck | 5 | 2 | 23 | 9 | 13 | 17 | 33 | 27 | 18 | – | 6 | 18 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 5 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 6 | – | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 11 | 1 | 4 | 1 | 1 | 2 | 2 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 1 | 7 | 8 |
Cytoplasm | 198 | 138 | 336 | 268 | 353 | 472 | 490 | 447 | 377 | – | 341 | 320 | 210 | 311 | 410 | 13 | 55 | 12 | 168 | 106 | 216 | 116 | 258 | 362 |
Endoplasmic Reticulum | 1 | 1 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | – | 0 | 1 | 2 | 0 | 2 | 0 | 5 | 1 | 1 | 0 | 1 | 2 | 4 | 4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 1 | 0 | 6 | 8 | 0 | 2 | 2 | 4 | 2 | 2 | 1 | 3 | 1 |
Golgi | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 1 | 1 |
Mitochondria | 24 | 17 | 1 | 5 | 18 | 74 | 71 | 35 | 168 | – | 203 | 117 | 18 | 7 | 9 | 0 | 5 | 0 | 18 | 4 | 44 | 29 | 78 | 59 |
Nucleus | 51 | 30 | 43 | 50 | 58 | 47 | 67 | 35 | 45 | – | 58 | 40 | 113 | 116 | 86 | 2 | 16 | 1 | 77 | 46 | 62 | 1 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 2 | 5 | 8 | 12 | 10 | – | 6 | 23 | 1 | 0 | 0 | 0 | 1 | 0 | 24 | 5 | 22 | 1 | 4 | 8 |
Vac/Vac Membrane | 26 | 22 | 3 | 2 | 16 | 9 | 2 | 7 | 1 | – | 9 | 7 | 13 | 45 | 84 | 3 | 13 | 5 | 36 | 23 | 50 | 7 | 22 | 20 |
Unique Cell Count | 292 | 195 | 398 | 331 | 442 | 564 | 620 | 532 | 540 | 537 | 469 | 341 | 468 | 582 | 16 | 82 | 19 | 352 | 206 | 439 | 171 | 414 | 505 | |
Labelled Cell Count | 306 | 211 | 420 | 338 | 469 | 627 | 683 | 570 | 635 | 634 | 540 | 360 | 486 | 601 | 18 | 98 | 21 | 352 | 206 | 439 | 171 | 414 | 505 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 3.9 | 4.6 | 4.1 | 4.6 | 3.5 | 3.7 | 4.1 | 3.1 | – | 3.1 | 3.7 | 4.1 | 4.1 | 4.2 | 6.5 | 6.4 | 7.1 | 3.7 | 3.6 | 3.9 |
Std Deviation (1e-4) | 0.5 | 1.3 | 0.7 | 0.9 | 1.2 | 0.7 | 0.7 | 1.1 | 0.7 | – | 0.7 | 1.0 | 1.2 | 1.0 | 1.3 | 2.6 | 2.0 | 2.5 | 1.5 | 1.5 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.16 | 0.02 | -0.4 | -0.3 | -0.17 | -0.54 | – | -0.56 | -0.31 | -0.15 | -0.16 | -0.13 | 0.52 | 0.48 | 0.63 | -0.3 | -0.35 | -0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.6741 | -1.2041 | -1.2859 | -1.3979 | -1.3775 | -1.6585 | -0.4564 | -0.3414 | 0.0318 | -0.209 | 0.2986 | -0.4837 | -0.5254 | -0.5904 | -0.2752 | -0.2903 | -0.4447 | -0.7641 |
Actin | 0.0391 | 0.0009 | 0.0042 | 0.0005 | 0.0008 | 0.0014 | 0.0261 | 0.0003 | 0.0127 | 0.0003 | 0.0138 | 0.0084 | 0.0165 | 0.0001 | 0.0087 | 0.0185 | 0.0015 | 0.0106 |
Bud | 0.0025 | 0.0006 | 0.0012 | 0.0001 | 0 | 0.0005 | 0.0032 | 0.0026 | 0.0009 | 0.0001 | 0.0035 | 0.001 | 0.0013 | 0.0001 | 0.0004 | 0.0003 | 0.0004 | 0.0002 |
Bud Neck | 0.0141 | 0.0042 | 0.0017 | 0.0006 | 0.0004 | 0.0023 | 0.0064 | 0.0004 | 0.0009 | 0.0009 | 0.0026 | 0.0031 | 0.0033 | 0.0002 | 0.0006 | 0.0025 | 0.0007 | 0.0008 |
Bud Periphery | 0.0058 | 0.0006 | 0.0011 | 0.0001 | 0 | 0.0005 | 0.0041 | 0.0024 | 0.0016 | 0.0001 | 0.0081 | 0.0027 | 0.0033 | 0.0001 | 0.0004 | 0.0004 | 0.0019 | 0.0012 |
Bud Site | 0.0054 | 0.0119 | 0.0071 | 0.0001 | 0 | 0.0002 | 0.0255 | 0.0036 | 0.0051 | 0.0005 | 0.0078 | 0.0006 | 0.0183 | 0.0006 | 0.0104 | 0.0086 | 0.0003 | 0.0001 |
Cell Periphery | 0.0007 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.001 | 0.0003 | 0.0002 | 0 | 0.0006 | 0.0002 | 0.0013 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 |
Cytoplasm | 0.07 | 0.122 | 0.1163 | 0.162 | 0.1619 | 0.1199 | 0.1795 | 0.1955 | 0.1187 | 0.1073 | 0.1528 | 0.28 | 0.0794 | 0.1544 | 0.0818 | 0.0667 | 0.0997 | 0.1493 |
Cytoplasmic Foci | 0.0311 | 0.0201 | 0.0122 | 0.0043 | 0.0171 | 0.0173 | 0.0314 | 0.0038 | 0.0119 | 0.014 | 0.0362 | 0.0185 | 0.0315 | 0.0034 | 0.0032 | 0.0016 | 0.0182 | 0.021 |
Eisosomes | 0.0011 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0016 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0083 | 0.0061 | 0.0035 | 0.0031 | 0.0016 | 0.0017 | 0.0097 | 0.002 | 0.0022 | 0.0026 | 0.005 | 0.0033 | 0.0048 | 0.0015 | 0.0015 | 0.0009 | 0.0017 | 0.0032 |
Endosome | 0.0205 | 0.0044 | 0.0049 | 0.0032 | 0.0027 | 0.0026 | 0.0414 | 0.0019 | 0.013 | 0.0122 | 0.1232 | 0.0059 | 0.0389 | 0.0013 | 0.0026 | 0.0012 | 0.0136 | 0.0084 |
Golgi | 0.0059 | 0.0004 | 0.0008 | 0.0001 | 0.0001 | 0.0002 | 0.008 | 0.0001 | 0.0081 | 0.0012 | 0.0726 | 0.002 | 0.0179 | 0.0001 | 0.0015 | 0.0004 | 0.0016 | 0.0041 |
Lipid Particles | 0.0119 | 0.0013 | 0.0041 | 0.0006 | 0.0003 | 0.0006 | 0.0117 | 0.0002 | 0.0044 | 0.0004 | 0.0177 | 0.007 | 0.0355 | 0.0007 | 0.0013 | 0.0003 | 0.0026 | 0.0067 |
Mitochondria | 0.0034 | 0.0004 | 0.0011 | 0.0008 | 0.0002 | 0.0006 | 0.0066 | 0.0006 | 0.0113 | 0.0013 | 0.034 | 0.0072 | 0.0359 | 0.0003 | 0.0011 | 0.0011 | 0.0061 | 0.0028 |
None | 0.6881 | 0.6003 | 0.3447 | 0.4134 | 0.6937 | 0.7707 | 0.5468 | 0.5003 | 0.2142 | 0.1338 | 0.2268 | 0.5508 | 0.5973 | 0.4992 | 0.3587 | 0.1991 | 0.6185 | 0.6807 |
Nuclear Periphery | 0.0135 | 0.0101 | 0.016 | 0.0139 | 0.0037 | 0.005 | 0.0125 | 0.0059 | 0.018 | 0.017 | 0.0135 | 0.0061 | 0.0104 | 0.0078 | 0.0214 | 0.0406 | 0.0137 | 0.0066 |
Nucleolus | 0.0047 | 0.0029 | 0.0052 | 0.0046 | 0.0006 | 0.0008 | 0.0078 | 0.0022 | 0.0031 | 0.0077 | 0.0031 | 0.001 | 0.0048 | 0.0015 | 0.0053 | 0.0045 | 0.005 | 0.001 |
Nucleus | 0.0382 | 0.1877 | 0.4229 | 0.3161 | 0.083 | 0.0622 | 0.0413 | 0.2631 | 0.5284 | 0.6708 | 0.2214 | 0.084 | 0.0469 | 0.3199 | 0.4805 | 0.6171 | 0.1507 | 0.0793 |
Peroxisomes | 0.0055 | 0.0041 | 0.0032 | 0.0005 | 0.0006 | 0.0007 | 0.0057 | 0.0001 | 0.0061 | 0.001 | 0.0269 | 0.0096 | 0.0316 | 0.0001 | 0.0006 | 0.0003 | 0.0367 | 0.0116 |
Punctate Nuclear | 0.021 | 0.0193 | 0.0462 | 0.073 | 0.0325 | 0.0113 | 0.01 | 0.0121 | 0.0325 | 0.0267 | 0.0138 | 0.0049 | 0.0108 | 0.0073 | 0.0183 | 0.0342 | 0.0215 | 0.0102 |
Vacuole | 0.0069 | 0.0021 | 0.0029 | 0.0025 | 0.0004 | 0.0012 | 0.0173 | 0.0023 | 0.0054 | 0.0016 | 0.0113 | 0.0026 | 0.0062 | 0.0011 | 0.0011 | 0.0009 | 0.0031 | 0.0011 |
Vacuole Periphery | 0.0023 | 0.0002 | 0.0006 | 0.0004 | 0.0001 | 0.0002 | 0.0034 | 0.0004 | 0.0015 | 0.0005 | 0.0051 | 0.0009 | 0.0026 | 0.0001 | 0.0004 | 0.0006 | 0.0023 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.6941 | 21.9195 | 18.6877 | 23.0603 | 24.6891 | 23.9851 | 19.6871 | 18.7775 | 18.9389 | 25.573 |
Translational Efficiency | 1.1895 | 0.8632 | 0.7471 | 0.8865 | 0.8469 | 1.057 | 0.7395 | 0.774 | 0.7455 | 0.7941 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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