Standard name
Human Ortholog
Description Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress; human CDC34 functionally complements the thermosensitivity of the cdc34-2 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.24 0.16 0.11 0.07 0.13 0.16 0.17 0.27 0.12 0.14 0.13 0.15 0.23 0.23 0.3 0.12 0.13 0.13 0.06 0.05 0.08 0.07 0.07 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0 0.3 0.21 0.38 0.31 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.49 0.38 0.47 0.72 0.68 0.54 0.5 0.51 0.47 0.49 0.46 0.42 0.46 0.41 0.33 0.67 0.55 0.43 0.81 0.8 0.76 0.73 0.7 0.61
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.57 0.67 0.52 0.24 0.3 0.38 0.37 0.28 0.29 0.23 0.17 0.27 0.36 0.43 0.42 0.28 0.39 0.5 0 0.08 0.07 0.06 0.1 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 0 3 5 2 0 0 0 0 0 0 0 9 12 2 1 4 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 2 4 3 0 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 56 63 14 6 19 23 43 36 25 39 20 28 30 34 51 24 23 17 14 23 13 4 9 9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 11 5 6 0 0 0 0 1 4
Endosome 1 1 0 0 0 1 2 2 0 3 0 2 0 1 1 0 1 2 3 4 1 1 0 4
Golgi 1 2 3 0 0 1 0 0 0 0 0 0 4 1 4 1 2 4 0 3 0 0 0 0
Mitochondria 0 0 0 0 0 3 12 3 63 60 57 57 0 0 1 4 4 0 0 2 1 0 1 0
Nucleus 117 152 63 58 102 80 126 69 98 138 69 79 59 61 57 136 96 58 195 347 119 45 96 111
Nuclear Periphery 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 1 1 0 1 0 1
Nucleolus 0 0 2 0 0 1 2 1 1 8 2 2 4 0 1 0 2 0 2 2 2 2 4 12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2 1 1 0 1 4
Vac/Vac Membrane 136 264 69 19 45 56 93 38 60 66 26 50 46 63 72 57 69 68 10 34 11 4 13 25
Unique Cell Count 237 396 133 80 149 147 251 134 207 281 150 186 129 147 172 204 175 135 242 436 157 62 137 183
Labelled Cell Count 311 483 151 83 166 166 278 151 254 325 178 221 144 161 187 233 202 155 242 436 157 62 137 183


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.4 13.1 13.3 12.4 12.3 8.8 8.0 7.7 6.2 6.7 5.7 6.3 14.2 15.1 14.0 13.0 16.4 18.4 – 14.2 15.1
Std Deviation (1e-4) 2.2 2.4 2.3 2.3 2.4 1.6 1.3 1.4 1.2 1.1 0.9 1.1 3.4 2.9 2.5 3.1 4.9 5.0 – 3.2 3.3
Intensity Change (Log2) – – – -0.1 -0.12 -0.59 -0.74 -0.79 -1.11 -0.99 -1.22 -1.07 0.09 0.18 0.07 -0.04 0.3 0.47 – 0.09 0.18

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70005101520WT3HU80HU120HU16005101520WT3rpd3Δ_1rpd3Δ_2rpd3Δ_305101520WT1AF140AF18005101520
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.7 0.6 1.3 1.7 3.4 0.4 1.0 0.7 1.2 2.8 2.8 4.0 0.4 0.7 0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 7.0 5.8 8.0 7.0 0 0 0 0 0 0
Nucleus 3.6 3.6 1.2 0.5 0.7 0 0.3 -0.2 -0.9 -0.3 -1.0 -2.5 3.5 1.3 -0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.0 -3.7 -2.3 -2.8 -3.9 -4.2 -5.8 -6.1 -4.6 -2.6 -1.5 -1.7 -4.4 -2.2 -0.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.6613 11.6106 10.3441 9.165 8.0165 10.086 9.6165 11.9569 11.1888 9.9541 9.5661 11.0246 8.5174 10.7558 9.6637 9.257 8.8275 9.5198
Actin 0.016 0 0.0077 0.0104 0.004 0 0.0357 0 0.0113 0.0244 0 0.0001 0.0028 0.0002 0.0005 0 0 0
Bud 0.0005 0.0001 0.0004 0.0001 0.001 0 0.0004 0 0.0011 0.0008 0 0 0 0.0002 0.0001 0 0 0
Bud Neck 0.0011 0.0004 0.002 0.0006 0.0015 0.0013 0.0011 0.0002 0.0006 0.0007 0.0001 0.0011 0.0014 0.0003 0.0003 0.0001 0.0001 0.0009
Bud Periphery 0.0007 0 0.0007 0.0003 0.0027 0 0.0005 0 0.001 0.0012 0 0 0 0.0001 0.0001 0 0 0
Bud Site 0.004 0.0002 0.0063 0.0032 0.0013 0 0.0021 0.0002 0.0175 0.0024 0 0 0.0002 0.0009 0.0001 0 0 0
Cell Periphery 0.0007 0.0001 0.0005 0.001 0.0008 0 0.0002 0 0.0001 0.0005 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.0167 0.0468 0.012 0.0055 0.013 0.0018 0.0268 0.0235 0.0109 0.0224 0.0083 0.0035 0.0068 0.0057 0.0049 0.0027 0.0008 0.0023
Cytoplasmic Foci 0.039 0.0002 0.0019 0.0031 0.0025 0 0.0269 0 0.013 0.0186 0 0.0007 0.0137 0 0.0005 0 0 0
Eisosomes 0.0002 0 0.0001 0.0022 0.0001 0 0.0003 0 0 0.0012 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0062 0.0002 0.0002 0.0038 0.006 0.0001 0.007 0.0002 0.0038 0.0092 0.0001 0.0002 0.0025 0.0001 0.0003 0.0001 0 0.0001
Endosome 0.0234 0.0003 0.0018 0.0012 0.0193 0 0.0116 0.0001 0.0073 0.0386 0.0001 0.0006 0.0104 0 0.001 0 0 0
Golgi 0.0102 0 0.0011 0.0011 0.025 0 0.0023 0 0.0012 0.0073 0 0 0.001 0 0.0002 0 0 0
Lipid Particles 0.0202 0 0.0013 0.0024 0.0036 0 0.0051 0 0.0032 0.0164 0 0.0001 0.0014 0 0.0005 0 0 0
Mitochondria 0.0049 0.0001 0.004 0.0008 0.0397 0.0001 0.0013 0.0001 0.0008 0.029 0.0001 0.0001 0.001 0.0001 0.0003 0.0001 0.0002 0.0001
None 0.0044 0 0.0005 0.0159 0.0028 0 0.0133 0 0.0006 0.0348 0 0.0005 0.0027 0.0001 0.0001 0 0 0
Nuclear Periphery 0.0218 0.0048 0.0035 0.0036 0.0216 0.0026 0.014 0.0062 0.0058 0.0234 0.0072 0.0039 0.0094 0.0034 0.003 0.0039 0.0021 0.0035
Nucleolus 0.0025 0.0012 0.0012 0.0008 0.0044 0.002 0.0021 0.0009 0.0008 0.0012 0.0006 0.0013 0.0011 0.0011 0.0008 0.0006 0.001 0.0013
Nucleus 0.783 0.9412 0.9404 0.9423 0.8177 0.9914 0.8308 0.9677 0.9115 0.7261 0.9827 0.9827 0.9339 0.9866 0.9852 0.9923 0.9953 0.9908
Peroxisomes 0.015 0 0.0101 0.0008 0.0006 0 0.0037 0 0.0009 0.0246 0 0 0.0005 0 0.0001 0 0 0
Punctate Nuclear 0.0179 0.0004 0.0003 0.0007 0.0011 0.0002 0.0121 0.0005 0.0079 0.0101 0.0004 0.0048 0.0093 0.0011 0.0015 0.0002 0.0002 0.0003
Vacuole 0.0093 0.0039 0.0038 0.0003 0.0229 0.0003 0.0021 0.0003 0.0005 0.0045 0.0002 0.0002 0.0008 0.0001 0.0003 0 0.0001 0.0005
Vacuole Periphery 0.0023 0.0002 0.0003 0.0001 0.0083 0 0.0007 0.0001 0.0002 0.0026 0.0001 0.0001 0.001 0 0.0001 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 48.4603 69.9794 66.7642 64.5246 54.7757 80.7225 64.2982 61.021 73.0035 57.4073
Translational Efficiency 1.6157 1.3543 1.2673 1.6634 1.2208 1.1054 1.264 1.376 1.197 1.2754

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1913 1105 2886 685 1917 1987 1601 71 3830 3092 4487 756

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1253.15 1111.72 1605.70 1678.58 1070.85 1248.56 1529.84 1761.77 1161.90 1199.66 1578.63 1686.39
Standard Deviation 194.13 207.91 241.65 316.03 164.16 194.11 248.86 346.80 201.54 209.67 246.94 319.97
Intensity Change Log 2 — -0.172766 0.357643 0.421682 — 0.221509 0.514624 0.718269 — 0.022348 0.432104 0.565945

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000245 0.000521 0.000210 0.000913 0.000313 0.000305 0.000626 0.000922 0.000279 0.000382 0.000358 0.000913
Bud Neck 0.007033 0.006016 0.009866 0.027495 0.005547 0.008535 0.011922 0.023064 0.006289 0.007635 0.010599 0.027079
Bud Site 0.000895 0.001862 0.000659 0.012997 0.001105 0.002617 0.001696 0.008911 0.001000 0.002347 0.001029 0.012613
Cell Periphery 0.000151 0.000195 0.000084 0.000253 0.000122 0.000128 0.000142 0.000453 0.000136 0.000152 0.000105 0.000272
Cytoplasm 0.017822 0.090038 0.007416 0.036665 0.034630 0.051459 0.007518 0.026039 0.026235 0.065246 0.007452 0.035667
Cytoplasmic Foci 0.000090 0.000764 0.000015 0.003984 0.000486 0.001405 0.000327 0.001547 0.000288 0.001176 0.000126 0.003755
Eisosomes 0.000012 0.000010 0.000015 0.000023 0.000009 0.000011 0.000029 0.000024 0.000011 0.000011 0.000020 0.000023
Endoplasmic Reticulum 0.003783 0.002929 0.001003 0.003658 0.003261 0.003383 0.001968 0.001842 0.003522 0.003221 0.001347 0.003487
Endosome 0.000172 0.000589 0.000044 0.007806 0.000205 0.001231 0.001036 0.000763 0.000189 0.001002 0.000398 0.007144
Golgi 0.000023 0.000159 0.000023 0.005980 0.000096 0.000655 0.000479 0.000196 0.000060 0.000478 0.000186 0.005437
Lipid Particles 0.000042 0.000172 0.000010 0.000496 0.000124 0.000186 0.000098 0.000624 0.000083 0.000181 0.000042 0.000508
Mitochondria 0.000561 0.001570 0.001051 0.011077 0.000850 0.001148 0.001501 0.002174 0.000706 0.001299 0.001211 0.010241
Mitotic Spindle 0.000480 0.002265 0.000287 0.138701 0.001312 0.003739 0.003712 0.070073 0.000896 0.003212 0.001509 0.132256
None 0.001946 0.008403 0.001990 0.006228 0.002402 0.003455 0.003898 0.006372 0.002174 0.005223 0.002671 0.006242
Nuclear Periphery 0.000677 0.000397 0.000596 0.010617 0.000553 0.000677 0.000974 0.000825 0.000615 0.000577 0.000731 0.009698
Nuclear Periphery Foci 0.000138 0.000643 0.000019 0.008898 0.000233 0.000438 0.000765 0.000319 0.000186 0.000511 0.000285 0.008093
Nucleolus 0.000612 0.000753 0.000191 0.004539 0.000397 0.001079 0.000540 0.000747 0.000504 0.000963 0.000316 0.004183
Nucleus 0.963397 0.879133 0.975977 0.676283 0.946544 0.913982 0.959449 0.845499 0.954962 0.901528 0.970080 0.692175
Peroxisomes 0.000026 0.000455 0.000009 0.000619 0.000057 0.000184 0.000054 0.000121 0.000041 0.000281 0.000025 0.000573
Vacuole 0.001757 0.002914 0.000506 0.030488 0.001636 0.004356 0.002557 0.009180 0.001697 0.003841 0.001238 0.028486
Vacuole Periphery 0.000136 0.000211 0.000029 0.012281 0.000117 0.001026 0.000710 0.000307 0.000126 0.000735 0.000272 0.011157

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.41 0.06 -12.08 -8.26 -12.32 -0.02 -6.44 -6.13 -6.29 -4.31 -2.95 -4.35 -12.42 -11.54 -11.25
Bud Neck 0.70 -7.44 -10.18 -10.01 -8.07 -2.85 -10.63 -5.84 -5.25 -3.94 -1.94 -12.95 -11.74 -10.68 -8.31
Bud Site -3.10 1.19 -6.14 -5.19 -6.26 -3.41 -3.23 -3.96 -3.46 -3.67 -4.57 -1.47 -6.84 -5.61 -6.69
Cell Periphery -1.94 4.29 -6.46 -4.83 -9.51 -0.19 -1.00 -3.64 -3.62 -3.57 -1.02 3.31 -7.92 -7.47 -9.19
Cytoplasm -14.16 8.00 -1.66 12.19 -6.11 -5.08 12.30 1.47 3.53 -1.94 -14.06 14.65 1.36 12.44 -6.47
Cytoplasmic Foci -3.75 3.41 -4.50 -1.68 -4.81 -2.13 1.61 -2.34 -0.89 -2.87 -3.26 2.38 -4.13 -0.68 -5.16
Eisosomes 2.21 -3.73 -12.89 -13.79 -11.84 -2.21 -18.49 -5.00 -4.81 -2.93 -0.35 -15.20 -14.43 -14.37 -10.23
Endoplasmic Reticulum 3.70 16.76 2.04 -0.49 -6.82 -1.21 6.25 0.06 0.63 -2.34 1.39 17.95 1.33 0.55 -6.02
Endosome -1.28 8.93 -3.88 -2.23 -4.22 -3.12 -1.33 -2.44 0.38 -1.00 -3.37 -0.43 -4.04 -1.57 -3.77
Golgi -2.07 -1.92 -2.85 -2.52 -2.84 -2.03 -1.14 -3.28 1.01 -0.45 -2.38 -0.98 -2.83 -1.72 -2.67
Lipid Particles -2.34 4.02 -8.47 -5.80 -8.80 -0.99 0.45 -1.56 -1.54 -1.57 -2.44 1.35 -3.84 -3.46 -4.00
Mitochondria -1.11 -10.74 -3.63 -2.42 -3.09 -0.83 -3.48 -5.42 -5.52 -4.02 -1.57 -5.14 -3.72 -3.15 -3.07
Mitotic Spindle -1.96 0.08 -6.58 -6.01 -6.59 -1.81 -1.66 -1.82 -1.67 -1.69 -2.79 -1.09 -6.75 -6.25 -6.65
None -5.00 -0.53 -7.42 1.21 -7.44 -2.66 -1.97 -1.70 -1.16 -1.19 -5.81 -1.50 -7.41 -1.82 -6.76
Nuclear Periphery 4.55 -0.07 -5.76 -6.13 -5.75 -1.76 -1.79 -3.35 -3.18 -2.88 0.24 -1.80 -6.22 -6.24 -5.96
Nuclear Periphery Foci -2.98 3.73 -3.07 -2.16 -3.28 -1.56 -0.86 -2.09 -1.06 -1.21 -2.89 0.27 -3.12 -2.39 -3.13
Nucleolus -0.59 2.30 -3.82 -3.51 -4.52 -2.58 -4.20 -4.03 -0.39 -2.36 -2.34 0.96 -4.20 -3.19 -4.38
Nucleus 13.69 -5.05 13.74 4.59 15.00 7.12 -2.94 4.27 3.13 4.62 14.69 -6.55 13.73 7.62 15.23
Peroxisomes -1.37 3.12 -4.04 0.53 -4.31 -2.46 -0.37 -3.48 -0.80 -3.53 -2.06 1.21 -4.04 -0.00 -4.31
Vacuole -2.63 3.34 -9.56 -9.17 -9.93 -5.92 -3.08 -3.53 -3.23 -3.44 -6.19 1.28 -10.27 -9.41 -10.37
Vacuole Periphery -0.64 1.40 -2.65 -2.56 -2.80 -2.49 -1.10 -1.47 1.96 0.43 -2.54 -0.32 -2.69 -1.80 -2.63
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress; human CDC34 functionally complements the thermosensitivity of the cdc34-2 mutant
Localization
Cell Percentages nucleus (36%), cytoplasm (3%), mixed (61%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cdc34

Cdc34


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc34-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available