Standard name
Human Ortholog
Description Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress; human CDC34 functionally complements the thermosensitivity of the cdc34-2 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.24 0.16 0.11 0.07 0.13 0.16 0.17 0.27 0.12 0.14 0.13 0.15 0.23 0.23 0.3 0.12 0.13 0.13 0.06 0.05 0.08 0.07 0.07 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0 0.3 0.21 0.38 0.31 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.49 0.38 0.47 0.72 0.68 0.54 0.5 0.51 0.47 0.49 0.46 0.42 0.46 0.41 0.33 0.67 0.55 0.43 0.81 0.8 0.76 0.73 0.7 0.61
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.57 0.67 0.52 0.24 0.3 0.38 0.37 0.28 0.29 0.23 0.17 0.27 0.36 0.43 0.42 0.28 0.39 0.5 0 0.08 0.07 0.06 0.1 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 0 3 5 2 0 0 0 0 0 0 0 9 12 2 1 4 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 2 4 3 0 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 56 63 14 6 19 23 43 36 25 39 20 28 30 34 51 24 23 17 14 23 13 4 9 9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 11 5 6 0 0 0 0 1 4
Endosome 1 1 0 0 0 1 2 2 0 3 0 2 0 1 1 0 1 2 3 4 1 1 0 4
Golgi 1 2 3 0 0 1 0 0 0 0 0 0 4 1 4 1 2 4 0 3 0 0 0 0
Mitochondria 0 0 0 0 0 3 12 3 63 60 57 57 0 0 1 4 4 0 0 2 1 0 1 0
Nucleus 117 152 63 58 102 80 126 69 98 138 69 79 59 61 57 136 96 58 195 347 119 45 96 111
Nuclear Periphery 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 1 1 0 1 0 1
Nucleolus 0 0 2 0 0 1 2 1 1 8 2 2 4 0 1 0 2 0 2 2 2 2 4 12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2 1 1 0 1 4
Vac/Vac Membrane 136 264 69 19 45 56 93 38 60 66 26 50 46 63 72 57 69 68 10 34 11 4 13 25
Unique Cell Count 237 396 133 80 149 147 251 134 207 281 150 186 129 147 172 204 175 135 242 436 157 62 137 183
Labelled Cell Count 311 483 151 83 166 166 278 151 254 325 178 221 144 161 187 233 202 155 242 436 157 62 137 183


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.4 13.1 13.3 12.4 12.3 8.8 8.0 7.7 6.2 6.7 5.7 6.3 14.2 15.1 14.0 13.0 16.4 18.4 – 14.2 15.1
Std Deviation (1e-4) 2.2 2.4 2.3 2.3 2.4 1.6 1.3 1.4 1.2 1.1 0.9 1.1 3.4 2.9 2.5 3.1 4.9 5.0 – 3.2 3.3
Intensity Change (Log2) – – – -0.1 -0.12 -0.59 -0.74 -0.79 -1.11 -0.99 -1.22 -1.07 0.09 0.18 0.07 -0.04 0.3 0.47 – 0.09 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.7 0.6 1.3 1.7 3.4 0.4 1.0 0.7 1.2 2.8 2.8 4.0 0.4 0.7 0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 7.0 5.8 8.0 7.0 0 0 0 0 0 0
Nucleus 3.6 3.6 1.2 0.5 0.7 0 0.3 -0.2 -0.9 -0.3 -1.0 -2.5 3.5 1.3 -0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -4.0 -3.7 -2.3 -2.8 -3.9 -4.2 -5.8 -6.1 -4.6 -2.6 -1.5 -1.7 -4.4 -2.2 -0.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.6613 11.6106 10.3441 9.165 8.0165 10.086 9.6165 11.9569 11.1888 9.9541 9.5661 11.0246 8.5174 10.7558 9.6637 9.257 8.8275 9.5198
Actin 0.016 0 0.0077 0.0104 0.004 0 0.0357 0 0.0113 0.0244 0 0.0001 0.0028 0.0002 0.0005 0 0 0
Bud 0.0005 0.0001 0.0004 0.0001 0.001 0 0.0004 0 0.0011 0.0008 0 0 0 0.0002 0.0001 0 0 0
Bud Neck 0.0011 0.0004 0.002 0.0006 0.0015 0.0013 0.0011 0.0002 0.0006 0.0007 0.0001 0.0011 0.0014 0.0003 0.0003 0.0001 0.0001 0.0009
Bud Periphery 0.0007 0 0.0007 0.0003 0.0027 0 0.0005 0 0.001 0.0012 0 0 0 0.0001 0.0001 0 0 0
Bud Site 0.004 0.0002 0.0063 0.0032 0.0013 0 0.0021 0.0002 0.0175 0.0024 0 0 0.0002 0.0009 0.0001 0 0 0
Cell Periphery 0.0007 0.0001 0.0005 0.001 0.0008 0 0.0002 0 0.0001 0.0005 0 0 0.0001 0 0 0 0 0
Cytoplasm 0.0167 0.0468 0.012 0.0055 0.013 0.0018 0.0268 0.0235 0.0109 0.0224 0.0083 0.0035 0.0068 0.0057 0.0049 0.0027 0.0008 0.0023
Cytoplasmic Foci 0.039 0.0002 0.0019 0.0031 0.0025 0 0.0269 0 0.013 0.0186 0 0.0007 0.0137 0 0.0005 0 0 0
Eisosomes 0.0002 0 0.0001 0.0022 0.0001 0 0.0003 0 0 0.0012 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0062 0.0002 0.0002 0.0038 0.006 0.0001 0.007 0.0002 0.0038 0.0092 0.0001 0.0002 0.0025 0.0001 0.0003 0.0001 0 0.0001
Endosome 0.0234 0.0003 0.0018 0.0012 0.0193 0 0.0116 0.0001 0.0073 0.0386 0.0001 0.0006 0.0104 0 0.001 0 0 0
Golgi 0.0102 0 0.0011 0.0011 0.025 0 0.0023 0 0.0012 0.0073 0 0 0.001 0 0.0002 0 0 0
Lipid Particles 0.0202 0 0.0013 0.0024 0.0036 0 0.0051 0 0.0032 0.0164 0 0.0001 0.0014 0 0.0005 0 0 0
Mitochondria 0.0049 0.0001 0.004 0.0008 0.0397 0.0001 0.0013 0.0001 0.0008 0.029 0.0001 0.0001 0.001 0.0001 0.0003 0.0001 0.0002 0.0001
None 0.0044 0 0.0005 0.0159 0.0028 0 0.0133 0 0.0006 0.0348 0 0.0005 0.0027 0.0001 0.0001 0 0 0
Nuclear Periphery 0.0218 0.0048 0.0035 0.0036 0.0216 0.0026 0.014 0.0062 0.0058 0.0234 0.0072 0.0039 0.0094 0.0034 0.003 0.0039 0.0021 0.0035
Nucleolus 0.0025 0.0012 0.0012 0.0008 0.0044 0.002 0.0021 0.0009 0.0008 0.0012 0.0006 0.0013 0.0011 0.0011 0.0008 0.0006 0.001 0.0013
Nucleus 0.783 0.9412 0.9404 0.9423 0.8177 0.9914 0.8308 0.9677 0.9115 0.7261 0.9827 0.9827 0.9339 0.9866 0.9852 0.9923 0.9953 0.9908
Peroxisomes 0.015 0 0.0101 0.0008 0.0006 0 0.0037 0 0.0009 0.0246 0 0 0.0005 0 0.0001 0 0 0
Punctate Nuclear 0.0179 0.0004 0.0003 0.0007 0.0011 0.0002 0.0121 0.0005 0.0079 0.0101 0.0004 0.0048 0.0093 0.0011 0.0015 0.0002 0.0002 0.0003
Vacuole 0.0093 0.0039 0.0038 0.0003 0.0229 0.0003 0.0021 0.0003 0.0005 0.0045 0.0002 0.0002 0.0008 0.0001 0.0003 0 0.0001 0.0005
Vacuole Periphery 0.0023 0.0002 0.0003 0.0001 0.0083 0 0.0007 0.0001 0.0002 0.0026 0.0001 0.0001 0.001 0 0.0001 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 48.4603 69.9794 66.7642 64.5246 54.7757 80.7225 64.2982 61.021 73.0035 57.4073
Translational Efficiency 1.6157 1.3543 1.2673 1.6634 1.2208 1.1054 1.264 1.376 1.197 1.2754

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress; human CDC34 functionally complements the thermosensitivity of the cdc34-2 mutant
Localization
Cell Percentages nucleus (36%), cytoplasm (3%), mixed (61%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cdc34

Cdc34


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cdc34-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available