Standard name
Human Ortholog
Description Putative protein of unknown function; subunit of evolutionarily conserved EMC (Endoplasmic Reticulum Membrane Complex) implicated in ERAD (ER-associated degradation) and proper assembly of multi-pass transmembrane (TM) proteins; EMC acts in yeast as an ER-mitochondria tether that interacts with outer membrane protein Tom5p of TOM (Translocase of the Mitochondrial Outer Membrane) complex; YDR056C is not an essential protein
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 – 0.06 0.07 0.1 0.05 – 0.07 0.08 0.1 0.09 0 0.06 0 0.12 0.09 0.16 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 – 0 0.06 0.08 0 0.11 0.15 0.2 0.41 0.34 0.2 0 0 0 0 0 0
Cytoplasm 0.16 0.16 – 0.19 0.13 0.22 0.19 – 0.18 0.15 0.12 0.15 0.06 0.05 0.06 0.18 0.19 0.16 0 0 0 0 0 0
Endoplasmic Reticulum 0.79 0.8 – 0.81 0.82 0.55 0.58 – 0.64 0.61 0.39 0.6 0.9 0.89 0.88 0 0 0 0.95 0.85 0.92 0.93 0.89 0.9
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0.06 0.06 0 0 – 0 0 0 0 0 0 0 0.26 0.34 0.45 0 0 0 0 0 0
Mitochondria 0.05 0 – 0.31 0 0.19 0 – 0.16 0.13 0.34 0.21 0 0 0 0.11 0 0.19 0 0 0 0 0 0
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.16 0 – 0.06 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 – 0 0.09 0.1 0.21 – 0.09 0.12 0.17 0.1 0 0 0 0 0.12 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 – 1 9 10 9 – 11 13 8 15 9 30 18 16 10 23 0 1 0 4 12 11
Bud 0 0 – 0 0 2 1 – 1 3 4 1 0 1 0 0 0 0 0 0 0 0 1 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 3 2 3 – 3 10 6 6 27 78 114 54 38 29 0 0 0 2 10 14
Cytoplasm 3 15 – 3 18 22 35 – 28 25 9 25 15 26 35 24 21 23 0 2 0 1 4 2
Endoplasmic Reticulum 15 74 – 13 111 55 109 – 101 105 30 100 212 460 511 1 5 4 61 97 26 275 391 441
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0
Golgi 0 0 – 1 8 0 4 – 0 5 0 0 10 23 11 34 38 65 0 0 0 0 6 4
Mitochondria 1 2 – 5 0 19 6 – 25 22 26 35 2 7 1 14 3 27 0 1 0 4 4 5
Nucleus 0 0 – 0 0 4 2 – 4 1 2 0 1 0 0 1 0 0 0 1 0 0 0 1
Nuclear Periphery 3 0 – 1 0 2 0 – 5 2 2 2 7 7 12 3 0 0 0 2 0 2 0 1
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 3 – 0 12 10 40 – 15 20 13 16 2 0 3 5 14 11 0 4 0 1 1 2
Unique Cell Count 19 92 16 136 100 189 158 172 77 167 235 518 582 131 112 143 65 115 29 295 441 489
Labelled Cell Count 24 96 24 161 126 209 193 206 100 200 285 633 705 152 129 182 65 115 29 295 441 489


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.8 17.1 – 13.1 16.1 9.2 12.7 – 10.5 11.2 9.9 10.8 18.7 18.8 19.3 21.2 21.6 21.3 17.4 17.8 18.2
Std Deviation (1e-4) 2.3 3.9 – 2.3 2.6 2.2 2.2 – 1.9 2.0 1.9 2.2 3.2 3.2 3.1 3.8 4.3 4.1 2.9 3.3 3.1
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.5952 10.2806 10.0905 9.0796 9.2545 9.8306 11.7706 13.6025 12.3641 12.5363 9.3181 13.464 14.6972 14.8162 14.1831 12.6783 13.0237 14.3646
Actin 0.0289 0.0003 0.0018 0.0003 0.0004 0.0061 0.0315 0.0113 0.0171 0.0001 0.0064 0.0001 0.0103 0.0091 0.0086 0.0001 0.0412 0.0059
Bud 0.0003 0 0.0025 0 0.0001 0.0001 0.0006 0.0001 0.0005 0 0.0005 0 0.0001 0 0.0001 0 0.0001 0
Bud Neck 0.0029 0.0001 0.0004 0.0001 0.0002 0.0002 0.0009 0.0001 0.0015 0 0.0003 0.0002 0.0005 0.0006 0.0003 0.0001 0.0001 0.0004
Bud Periphery 0.0006 0 0.0014 0.0002 0.0001 0.0001 0.0012 0.0002 0.0006 0 0.0006 0 0.0004 0.0001 0.0002 0 0.0001 0
Bud Site 0.0036 0 0.0049 0 0.0001 0.0001 0.003 0.001 0.0097 0 0.0007 0 0.0005 0.0012 0.0004 0 0.0002 0
Cell Periphery 0.0024 0.0027 0.0029 0.0038 0.0006 0.0006 0.0015 0.0023 0.0019 0.0003 0.0014 0.0003 0.001 0.0101 0.0028 0.0008 0.0006 0.0006
Cytoplasm 0.0162 0.0164 0.0136 0.0025 0.0114 0.0085 0.0035 0.0068 0.0092 0.0008 0.0023 0.0057 0.0145 0.0116 0.0088 0.0023 0.0022 0.0064
Cytoplasmic Foci 0.0158 0.0015 0.0013 0.0003 0.0008 0.002 0.0175 0.0012 0.0089 0.0001 0.014 0.0004 0.0062 0.0009 0.0013 0.0001 0.0008 0.0014
Eisosomes 0.0037 0 0.0003 0.0003 0 0 0.0014 0.0001 0.0003 0 0.0001 0 0.0002 0.0003 0.0002 0 0.0001 0.0001
Endoplasmic Reticulum 0.7941 0.9478 0.9393 0.9438 0.9398 0.9494 0.7633 0.9564 0.9218 0.9874 0.693 0.9756 0.8917 0.9478 0.9475 0.9774 0.9448 0.971
Endosome 0.0254 0.0102 0.0044 0.0042 0.0224 0.0061 0.034 0.002 0.0046 0.0036 0.0766 0.0012 0.0066 0.0011 0.0066 0.0011 0.0012 0.0012
Golgi 0.0109 0.0052 0.0021 0.0021 0.0085 0.0023 0.0257 0.0014 0.0068 0.0009 0.0882 0.0003 0.0064 0.0023 0.0029 0.0002 0.0015 0.0013
Lipid Particles 0.0164 0.0068 0.0025 0.011 0.0009 0.005 0.0289 0.0097 0.0053 0.0003 0.0257 0.0022 0.0076 0.0091 0.0097 0.0003 0.0014 0.0007
Mitochondria 0.0165 0.0005 0.001 0.0183 0.0034 0.0013 0.0168 0.0001 0.0005 0.0002 0.033 0 0.0025 0.0001 0.0006 0 0.0004 0.0004
None 0.0071 0.0001 0.0032 0.0001 0.0001 0.0009 0.0077 0.0001 0.0013 0 0.0003 0 0.0067 0 0.0005 0.0001 0.0002 0.0045
Nuclear Periphery 0.0309 0.0051 0.0052 0.0062 0.0045 0.0112 0.0239 0.0052 0.0034 0.0046 0.004 0.0121 0.0245 0.0034 0.0054 0.0149 0.0024 0.0034
Nucleolus 0.001 0 0.0015 0 0 0.0001 0.0012 0 0.0003 0 0.0001 0 0.0008 0 0.0001 0 0 0
Nucleus 0.0031 0.0003 0.0022 0.0002 0.0004 0.0007 0.0015 0.0002 0.0004 0.0001 0.0003 0.0004 0.0024 0.0001 0.0006 0.001 0.0001 0.0007
Peroxisomes 0.0051 0 0.0001 0 0 0.0004 0.0175 0.0001 0.0013 0 0.0457 0 0.0016 0 0.0001 0 0.0014 0.0001
Punctate Nuclear 0.0047 0.0001 0.0013 0 0.0001 0.0014 0.0036 0 0.0003 0 0.0004 0 0.0123 0 0.0002 0 0.0004 0.0004
Vacuole 0.0075 0.0026 0.0073 0.0041 0.0032 0.0026 0.0114 0.0014 0.0039 0.0011 0.0042 0.0011 0.0019 0.0019 0.0026 0.0013 0.0009 0.0013
Vacuole Periphery 0.0028 0.0003 0.0006 0.0022 0.0028 0.0009 0.0033 0.0002 0.0003 0.0005 0.0023 0.0004 0.0013 0.0001 0.0005 0.0003 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 43.7434 36.7098 43.8213 47.6407 53.2428 46.3958 45.1862 36.4882 52.5913 46.9869
Translational Efficiency 1.9698 1.8835 1.3372 1.47 0.9975 1.5514 1.3688 1.3877 1.142 1.241

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
787 890 259 1280 1796 2022 2073 1906 2583 2912 2332 3186

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1241.61 1306.83 1915.21 1623.10 1397.46 1487.11 1740.67 1863.00 1349.97 1432.01 1760.06 1766.62
Standard Deviation 206.94 282.87 278.44 289.95 237.48 236.41 251.27 296.05 239.60 264.88 260.27 316.29
Intensity Change Log 2 — 0.073859 0.625291 0.386540 — 0.089704 0.316836 0.414821 — 0.082271 0.470189 0.401584

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001160 0.001617 0.000341 0.003002 0.001107 0.000872 0.002315 0.000796 0.001123 0.001100 0.002095 0.001682
Bud Neck 0.000362 0.000586 0.000729 0.001005 0.000084 0.000114 0.000520 0.000482 0.000169 0.000258 0.000544 0.000692
Bud Site 0.002318 0.008944 0.001182 0.008566 0.000949 0.002014 0.003812 0.004851 0.001366 0.004132 0.003520 0.006343
Cell Periphery 0.005539 0.003018 0.005900 0.023021 0.002106 0.003236 0.007628 0.028323 0.003152 0.003170 0.007436 0.026193
Cytoplasm 0.000751 0.011709 0.000469 0.003257 0.000778 0.004412 0.003942 0.003633 0.000770 0.006642 0.003556 0.003482
Cytoplasmic Foci 0.001427 0.018674 0.000075 0.002299 0.000738 0.001821 0.001162 0.002217 0.000948 0.006972 0.001041 0.002250
Eisosomes 0.000191 0.000094 0.000061 0.000372 0.000076 0.000122 0.000206 0.000320 0.000111 0.000114 0.000189 0.000340
Endoplasmic Reticulum 0.972580* 0.841267* 0.971066* 0.911866* 0.985361* 0.963150* 0.953653* 0.900447* 0.981467* 0.925898* 0.955587* 0.905035*
Endosome 0.004556 0.033850 0.004159 0.007531 0.002887 0.005701 0.004773 0.005566 0.003395 0.014304 0.004705 0.006356
Golgi 0.003115 0.020841 0.001795 0.006331 0.001281 0.002746 0.002472 0.006347 0.001840 0.008276 0.002397 0.006341
Lipid Particles 0.001306 0.019879 0.006137 0.020460 0.001504 0.006707 0.012595 0.031394 0.001444 0.010733 0.011878 0.027001
Mitochondria 0.000353 0.001694 0.000049 0.000705 0.000241 0.001093 0.000452 0.000898 0.000275 0.001276 0.000407 0.000820
Mitotic Spindle 0.000131 0.000362 0.000149 0.000922 0.000012 0.000031 0.000055 0.000482 0.000048 0.000132 0.000066 0.000659
None 0.000121 0.003100 0.000027 0.001036 0.001035 0.004003 0.000544 0.000815 0.000756 0.003727 0.000487 0.000904
Nuclear Periphery 0.000239 0.000559 0.000993 0.000738 0.000163 0.000147 0.000299 0.000986 0.000186 0.000273 0.000376 0.000887
Nuclear Periphery Foci 0.001313 0.013958 0.002067 0.002024 0.000592 0.000981 0.000983 0.001144 0.000811 0.004947 0.001103 0.001498
Nucleolus 0.000234 0.000525 0.000001 0.000007 0.000001 0.000006 0.000004 0.000013 0.000072 0.000165 0.000004 0.000010
Nucleus 0.002169 0.002539 0.000030 0.000149 0.000023 0.000113 0.000116 0.000170 0.000676 0.000855 0.000106 0.000161
Peroxisomes 0.000254 0.000611 0.000005 0.000930 0.000504 0.001225 0.001275 0.002150 0.000428 0.001037 0.001134 0.001660
Vacuole 0.001124 0.011322 0.004302 0.004065 0.000373 0.001124 0.002509 0.007130 0.000602 0.004241 0.002708 0.005899
Vacuole Periphery 0.000756 0.004852 0.000463 0.001713 0.000186 0.000383 0.000685 0.001836 0.000360 0.001749 0.000661 0.001787

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.80 1.88 -2.35 -1.68 -3.31 0.37 -1.52 0.39 0.03 2.43 0.05 -1.54 -1.23 -1.66 0.53
Bud Neck -1.20 -1.29 -2.09 -1.55 -0.67 -0.75 -3.96 -5.31 -4.99 0.18 -1.41 -3.37 -4.21 -3.74 -1.04
Bud Site -4.09 0.63 -4.07 0.11 -4.23 -1.87 -3.45 -3.83 -2.68 -0.90 -4.34 -2.93 -5.81 -2.37 -2.67
Cell Periphery 2.00 -0.21 -7.66 -10.16 -7.45 -1.86 -6.81 -15.33 -14.59 -11.48 -0.03 -5.44 -17.12 -17.65 -13.26
Cytoplasm -6.41 0.28 -3.69 4.71 -3.81 -7.44 -5.52 -6.30 1.01 0.37 -9.39 -5.40 -7.22 4.29 0.09
Cytoplasmic Foci -7.94 2.48 -1.27 7.62 -5.12 -2.72 -1.17 -3.46 -0.91 -2.63 -8.22 -0.30 -3.59 6.42 -3.61
Eisosomes 1.77 2.38 -2.22 -4.41 -4.97 -2.10 -4.05 -4.12 -3.41 -1.83 -0.12 -2.52 -4.93 -5.29 -3.15
Endoplasmic Reticulum 13.74 0.40 9.54 -6.94 7.40 7.57 9.94 18.52 12.59 10.28 14.98 8.55 20.95 4.59 12.06
Endosome -13.41 0.15 -3.29 12.09 -3.28 -4.72 -4.64 -6.80 0.19 -1.55 -13.28 -3.35 -7.29 9.39 -3.50
Golgi -8.03 1.21 -2.68 7.14 -6.77 -3.49 -3.37 -6.36 -4.19 -4.66 -8.58 -1.30 -7.22 2.30 -6.73
Lipid Particles -7.62 -1.50 -9.76 -0.22 -3.66 -5.57 -8.56 -13.70 -10.52 -7.47 -9.35 -8.67 -16.73 -9.04 -7.83
Mitochondria -2.27 2.76 -1.96 1.64 -4.51 -4.16 -2.39 -4.12 0.76 -2.64 -4.37 -1.64 -4.64 1.81 -3.27
Mitotic Spindle -1.69 -0.47 -1.16 -0.81 -0.95 -3.18 -4.96 -2.27 -2.18 -2.05 -2.00 -0.79 -2.04 -1.75 -1.95
None -2.42 1.61 -1.69 1.53 -1.87 -3.40 1.10 0.44 3.93 -0.84 -4.17 0.84 -0.36 4.01 -1.36
Nuclear Periphery -2.84 -5.37 -4.72 -1.43 1.74 0.62 -4.37 -13.12 -13.71 -10.69 -2.21 -5.41 -12.84 -10.69 -9.17
Nuclear Periphery Foci -6.44 -1.33 -1.22 6.08 0.10 -2.10 -2.74 -2.93 -0.75 -0.75 -6.64 -1.66 -2.86 5.39 -1.75
Nucleolus -0.58 1.48 1.44 1.10 -5.87 -4.02 -5.09 -9.90 -3.92 -6.55 -0.60 1.42 1.28 1.07 -6.90
Nucleus -0.16 1.65 1.55 1.52 -4.72 -4.79 -5.63 -7.58 -2.36 -2.24 -0.26 1.43 1.28 1.43 -2.85
Peroxisomes -1.91 3.25 -2.92 -1.15 -4.24 -2.46 -1.98 -4.45 -2.43 -1.91 -2.88 -2.15 -4.94 -2.39 -1.45
Vacuole -8.11 -3.47 -5.62 5.31 0.05 -2.69 -9.07 -9.22 -7.73 -6.07 -8.28 -8.56 -11.03 -2.93 -6.25
Vacuole Periphery -6.79 1.14 -1.54 3.90 -2.11 -2.44 -1.47 -4.11 -3.56 -2.19 -7.07 -0.96 -4.19 -0.11 -2.50
Standard name
Human Ortholog
Description Putative protein of unknown function; subunit of evolutionarily conserved EMC (Endoplasmic Reticulum Membrane Complex) implicated in ERAD (ER-associated degradation) and proper assembly of multi-pass transmembrane (TM) proteins; EMC acts in yeast as an ER-mitochondria tether that interacts with outer membrane protein Tom5p of TOM (Translocase of the Mitochondrial Outer Membrane) complex; YDR056C is not an essential protein
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Emc10

Emc10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Emc10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available