Standard name
Human Ortholog
Description Constituent of 66S pre-ribosomal particles; required for large (60S) ribosomal subunit biogenesis; acts as part of a Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription and has a role in bigenesis of the large ribosomal subunit; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0.05 0.05 0.07 – 0.05 0.08 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – 0 0 0.09 0.12 0.11 0 0 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0.08 0.06 0.06 0.2 0.11 0.11 0.26 – 0.21 0.27 0 0 0 0.17 0 0.15 0 0 0 0 0 0
Nucleus 0.27 0.27 0.14 0 0.05 0.05 0 0.05 0.05 – 0.06 0.06 0.07 0.05 0 0.11 0.1 0.12 0.13 0.23 0.06 0.07 0 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.71 0.75 0.86 0.92 0.89 0.84 0.82 0.83 0.81 – 0.8 0.82 0.87 0.79 0.81 0.79 0.83 0.81 0.73 0.64 0.75 0.79 0.72 0.68
Peroxisomes 0 0 0 0 0 0 0.06 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0.27 0.32 0.33 0.44 0.4 0.35 – 0.35 0.44 0.12 0.13 0.16 0 0 0 0 0 0.07 0.05 0.09 0.11
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0.07 0 0.05 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 0 7 6 6 – 8 2 1 0 1 0 0 0 2 2 2 1 5 6
Bud Neck 0 1 3 9 4 4 8 7 5 – 6 3 10 18 11 1 0 0 2 2 1 2 8 8
Bud Site 0 0 2 1 3 11 17 20 20 – 12 21 1 1 6 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 1 1 0 0 0 0 0 0 0 0 0
Cytoplasm 2 3 5 5 4 8 10 6 9 – 8 2 26 50 51 16 7 17 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 4 2 2 0 0 0 0 1 0
Endosome 0 4 3 0 16 5 10 7 3 – 6 3 4 12 8 6 2 4 0 1 9 4 5 11
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 2 0 0 0 0 1 1
Mitochondria 3 20 31 16 14 49 34 42 78 – 53 69 11 6 15 68 8 40 3 4 13 2 4 10
Nucleus 65 66 52 9 11 13 9 19 16 – 16 16 20 22 19 41 19 31 34 60 25 15 13 19
Nuclear Periphery 6 2 7 1 0 0 1 1 4 – 0 0 0 2 1 1 0 1 11 1 4 0 1 3
Nucleolus 170 185 325 242 215 204 261 315 241 – 202 213 256 343 369 307 165 214 199 167 301 171 243 293
Peroxisomes 2 1 0 5 6 7 19 13 7 – 3 10 14 14 9 5 3 1 0 0 1 1 4 7
SpindlePole 1 3 23 70 77 80 142 154 104 – 88 114 35 57 74 14 5 5 3 10 26 10 28 46
Vac/Vac Membrane 9 5 2 0 0 0 1 0 2 – 0 0 2 4 2 22 13 8 12 10 12 8 11 11
Unique Cell Count 241 247 379 262 242 244 320 381 299 251 260 293 434 457 390 199 264 272 264 402 219 336 434
Labelled Cell Count 258 290 453 358 350 381 519 590 495 402 453 380 530 567 486 224 325 272 264 402 219 336 434


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 24.7 18.0 13.2 11.7 10.8 8.3 8.1 7.7 7.0 – 6.6 6.5 16.9 15.3 13.3 24.3 26.5 25.2 20.0 19.4 19.5
Std Deviation (1e-4) 6.7 6.4 4.3 3.2 2.6 2.1 1.9 2.0 1.9 – 1.9 1.9 6.8 7.0 6.1 10.0 9.6 9.2 7.7 6.4 6.1
Intensity Change (Log2) – – – -0.18 -0.3 -0.66 -0.7 -0.78 -0.91 – -1.01 -1.03 0.36 0.21 0.01 0.88 1.0 0.94 0.6 0.55 0.56

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP620RAP7000102030WT3HU80HU120HU1600102030WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30102030WT1AF100AF140AF1800102030
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 1.8 0 0 – 0 0 2.4 3.0 1.8 0 0 0
Bud Site 0 0 3.4 3.9 3.9 4.5 – 3.5 5.0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0 0 1.7 1.6 0.3 1.5 – 1.6 0 4.6 5.8 5.7 2.4 0 3.5
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 4.1 0 2.3 0 0 – 0 0 0 2.1 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria -1.0 -1.1 4.3 1.1 1.3 6.3 – 4.7 6.3 -2.4 -4.6 -3.1 3.8 -1.9 2.8
Nucleus -4.4 -3.7 -3.3 -5.1 -4.1 -3.6 – -2.9 -3.0 -2.9 -4.3 -4.9 -1.4 -1.5 -0.7
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 2.6 1.1 -0.7 -1.5 -1.2 -1.8 – -1.8 -1.3 0.6 -2.5 -1.9 -2.5 -0.9 -1.6
Peroxisomes 0 0 0 4.8 3.6 0 – 0 0 4.3 3.5 0 0 0 0
SpindlePole 7.3 8.5 8.8 11.9 11.2 9.5 – 9.4 11.4 2.7 3.4 4.6 -1.6 -1.9 -2.6
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 4.1 4.3 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 16.1626 21.3294 19.6032 17.9241 18.0698 18.8047 14.3075 17.4067 16.2271 15.1679 15.8423 15.4054 19.6661 22.9486 21.7497 19.9478 19.5091 20.3704
Actin 0.0091 0 0 0 0 0 0.0025 0 0.0023 0.0001 0 0.0001 0.0303 0 0 0 0.0001 0
Bud 0.0004 0 0.0001 0 0 0 0.0001 0 0.0001 0.0001 0 0 0.0001 0 0 0 0.0001 0
Bud Neck 0.0005 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0003 0.0001
Bud Periphery 0.0004 0 0.0001 0 0.0001 0 0.0001 0 0.0002 0.0001 0 0.0001 0.0001 0 0 0 0.0001 0
Bud Site 0.0027 0.0002 0.0006 0 0.0002 0 0.0002 0.0001 0.0003 0.0003 0 0.0001 0.0004 0.0001 0 0.0001 0.0002 0.0001
Cell Periphery 0.0003 0.0002 0.0016 0.0002 0.0001 0.0003 0.0002 0.0003 0.0001 0.0003 0.0001 0.0002 0.0004 0.0005 0.0001 0.0002 0.0001 0.0002
Cytoplasm 0.0027 0.0001 0 0 0 0 0.0011 0 0.0001 0.0001 0 0.0002 0.001 0 0 0 0 0
Cytoplasmic Foci 0.0208 0.0042 0.0028 0.0001 0.0005 0.0005 0.0032 0.0002 0.0039 0.0017 0.0011 0.0016 0.0217 0.0003 0.0005 0.0002 0.0126 0.0007
Eisosomes 0.0004 0 0 0 0 0 0.0001 0 0.0001 0.0001 0 0 0.0002 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0004 0 0 0 0 0 0.001 0 0.0001 0.0001 0 0.0001 0.0004 0 0 0 0 0
Endosome 0.0124 0.0055 0.0003 0 0 0 0.0052 0 0.0007 0.0001 0 0.0002 0.0036 0.0002 0 0 0.0001 0
Golgi 0.0024 0.0003 0.0001 0 0.0001 0 0.0014 0 0.0003 0.0001 0 0.0002 0.0016 0.0002 0 0 0.0001 0.0001
Lipid Particles 0.0219 0.0029 0.0039 0.0001 0.0017 0.0051 0.0103 0.0011 0.0021 0.0024 0.004 0.013 0.0077 0.0089 0.0009 0.0005 0.0018 0.0102
Mitochondria 0.0184 0.0008 0.0025 0.0002 0.0027 0.0313 0.0051 0.0001 0.0016 0.0024 0.0001 0.0121 0.0018 0.0009 0.0003 0.0002 0.009 0.0091
None 0.002 0 0.0002 0 0.0001 0 0.0008 0 0.0006 0.0006 0 0.0002 0.0059 0 0 0 0.0001 0
Nuclear Periphery 0.0047 0.0001 0.0001 0 0.0003 0.0001 0.0041 0.0018 0.0001 0.0012 0.0003 0.0013 0.0022 0.0001 0.0007 0 0.0012 0.0003
Nucleolus 0.7841 0.8524 0.9155 0.9257 0.9224 0.903 0.9087 0.906 0.9225 0.8899 0.9135 0.9104 0.8462 0.9045 0.9324 0.9555 0.8845 0.9098
Nucleus 0.0805 0.1308 0.0656 0.0732 0.0693 0.0559 0.05 0.0866 0.0627 0.0919 0.0728 0.0565 0.0505 0.0776 0.0608 0.0427 0.0842 0.061
Peroxisomes 0.0045 0.0003 0.0003 0 0.0002 0.0001 0.0023 0 0.0007 0.0002 0 0.0002 0.0029 0.0001 0 0 0.0009 0.0002
Punctate Nuclear 0.0268 0.0001 0.0005 0.0001 0.0015 0.0013 0.0023 0.0007 0.0009 0.0062 0.0078 0.0015 0.0211 0.0009 0.0039 0.0002 0.0042 0.0012
Vacuole 0.0031 0.0014 0.0041 0.0002 0.0002 0.0004 0.0005 0.0009 0.0003 0.001 0.0001 0.0004 0.0006 0.0017 0.0002 0.0002 0.0002 0.0005
Vacuole Periphery 0.0016 0.0005 0.0014 0.0001 0.0004 0.0017 0.0008 0.0021 0.0002 0.001 0.0001 0.0018 0.001 0.0039 0.0001 0.0001 0.0004 0.0063

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 101.0916 96.7789 79.8992 64.928 84.3587 55.8921 62.9403 49.2051 42.8649 75.4531
Translational Efficiency 1.1838 1.2685 1.1292 1.1179 1.0562 1.3151 1.0003 1.0387 1.0474 1.0181

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
124 1348 125 706 1686 1012 981 118 1810 2360 1106 824

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1344.30 1603.96 2088.40 1739.18 1329.33 1599.20 1733.29 2278.13 1330.36 1601.92 1773.42 1816.36
Standard Deviation 365.76 432.30 411.82 539.46 366.39 355.96 570.15 581.76 366.37 401.35 565.81 577.45
Intensity Change Log 2 — 0.254783 0.635543 0.371552 — 0.266651 0.382814 0.777151 — 0.260696 0.515411 0.587430

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000626 0.000424 0.000288 0.000713 0.000512 0.000477 0.000516 0.000635 0.000519 0.000447 0.000490 0.000702
Bud Neck 0.002956 0.001545 0.003032 0.016480 0.001853 0.002742 0.004096 0.012050 0.001929 0.002058 0.003976 0.015846
Bud Site 0.001811 0.000302 0.001820 0.003989 0.000698 0.000818 0.001203 0.003156 0.000775 0.000523 0.001273 0.003869
Cell Periphery 0.000085 0.000030 0.000014 0.000213 0.000066 0.000042 0.000073 0.000089 0.000067 0.000035 0.000066 0.000195
Cytoplasm 0.000340 0.000079 0.000212 0.003221 0.000386 0.000448 0.000847 0.000243 0.000383 0.000237 0.000775 0.002795
Cytoplasmic Foci 0.011091 0.003963 0.007451 0.010468 0.005912 0.001944 0.005565 0.018975 0.006267 0.003097 0.005778 0.011686
Eisosomes 0.000259 0.000297 0.000229 0.000179 0.000539 0.000222 0.000257 0.000199 0.000520 0.000265 0.000253 0.000182
Endoplasmic Reticulum 0.000504 0.000195 0.000092 0.000472 0.000295 0.000331 0.000188 0.000154 0.000309 0.000253 0.000177 0.000426
Endosome 0.000157 0.000270 0.000033 0.003836 0.000085 0.000491 0.000109 0.000199 0.000090 0.000365 0.000101 0.003315
Golgi 0.000219 0.000131 0.000385 0.001306 0.000158 0.000383 0.000259 0.000738 0.000162 0.000240 0.000273 0.001225
Lipid Particles 0.000190 0.000140 0.000032 0.001886 0.000101 0.000437 0.000039 0.000137 0.000107 0.000267 0.000038 0.001635
Mitochondria 0.001584 0.001686 0.001239 0.005962 0.002032 0.001715 0.002311 0.003712 0.002001 0.001699 0.002190 0.005640
Mitotic Spindle 0.028158 0.001658 0.008809 0.014720 0.001527 0.006753 0.006435 0.046189 0.003352 0.003843 0.006704 0.019227
None 0.000744 0.000662 0.000264 0.002065 0.001967 0.000406 0.001318 0.000547 0.001884 0.000553 0.001199 0.001848
Nuclear Periphery 0.002532 0.000493 0.000236 0.001182 0.000382 0.001363 0.000319 0.002997 0.000530 0.000866 0.000309 0.001442
Nuclear Periphery Foci 0.007840 0.001169 0.000285 0.001021 0.000872 0.002342 0.000250 0.001275 0.001350 0.001672 0.000254 0.001057
Nucleolus 0.649353 0.694818 0.697199 0.556584 0.673273 0.677494 0.665667 0.679567 0.671634 0.687389 0.669231 0.574195
Nucleus 0.240642 0.232828 0.097232 0.326130 0.248288 0.274935 0.234963 0.157075 0.247764 0.250884 0.219397 0.301920
Peroxisomes 0.041177 0.057046 0.179844 0.036692 0.058536 0.023891 0.072124 0.060523 0.057347 0.042829 0.084298 0.040105
Vacuole 0.002907 0.000951 0.000691 0.007678 0.001199 0.001183 0.001794 0.006205 0.001316 0.001050 0.001669 0.007468
Vacuole Periphery 0.006827 0.001311 0.000614 0.005204 0.001317 0.001583 0.001667 0.005334 0.001695 0.001428 0.001548 0.005223

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.93 1.66 -1.29 -7.58 -3.64 0.67 -0.59 -2.11 -2.31 -1.83 2.49 0.06 -5.63 -7.43 -5.13
Bud Neck 1.62 -0.23 -7.17 -12.01 -7.89 -2.41 -6.62 -5.16 -4.70 -4.14 -0.51 -6.30 -12.63 -12.49 -10.53
Bud Site 1.74 -0.60 -1.87 -8.49 -0.03 -0.59 -2.04 -4.09 -3.56 -3.42 0.76 -1.76 -7.50 -8.29 -4.66
Cell Periphery 1.46 1.57 -1.81 -6.50 -6.62 0.90 -0.46 -1.04 -1.80 -0.68 1.67 -0.12 -4.48 -6.50 -4.52
Cytoplasm 1.09 -0.16 -2.48 -3.21 -2.20 -0.56 -0.83 0.42 0.81 0.96 0.60 -0.86 -2.87 -2.99 -2.11
Cytoplasmic Foci 1.56 0.95 0.41 -4.81 -1.99 4.80 0.14 -2.75 -3.42 -2.78 3.73 0.49 -4.03 -6.49 -4.52
Eisosomes -0.47 0.33 1.05 5.84 3.37 8.12 8.27 10.06 0.63 2.01 8.27 8.36 12.33 4.78 3.84
Endoplasmic Reticulum 2.20 2.01 -0.60 -4.29 -3.50 -0.80 3.84 1.67 1.83 -0.19 1.48 4.29 -2.89 -3.42 -4.36
Endosome -0.46 1.09 -2.64 -2.53 -2.72 -2.53 -0.63 -2.25 1.22 -1.80 -1.97 -0.44 -2.73 -2.45 -2.71
Golgi 2.32 -2.48 -2.97 -3.36 -2.49 -1.34 -3.35 -4.55 -2.08 -3.80 -1.01 -3.96 -3.59 -3.24 -3.16
Lipid Particles 0.59 2.06 -1.62 -1.68 -1.77 -1.45 1.75 -1.35 1.02 -2.19 -1.48 2.09 -1.73 -1.52 -1.80
Mitochondria -0.27 0.85 -3.22 -3.19 -3.48 0.97 -1.41 -3.47 -3.92 -2.82 1.25 -1.14 -3.16 -3.43 -2.94
Mitotic Spindle 2.22 1.41 1.15 -4.28 -0.74 -2.79 -3.34 -3.02 -2.60 -2.68 -0.57 -2.17 -4.57 -4.34 -3.43
None 0.56 1.78 -3.86 -4.32 -4.98 3.17 1.22 2.89 -1.47 3.00 2.93 1.36 0.11 -4.76 -1.85
Nuclear Periphery 2.02 2.23 1.22 -2.48 -3.14 -2.57 0.42 -1.41 -0.81 -1.44 -1.66 1.44 -2.64 -1.57 -3.24
Nuclear Periphery Foci 1.73 1.97 1.74 0.06 -2.86 -1.82 3.04 -0.50 1.16 -1.49 -0.67 3.35 0.51 1.18 -3.59
Nucleolus -2.06 -1.01 3.65 11.45 6.16 -0.28 1.12 0.54 0.63 0.12 -2.07 0.81 9.31 10.77 8.32
Nucleus 0.30 5.08 -3.98 -7.86 -12.66 -2.66 1.33 4.54 5.63 3.81 -0.41 2.99 -5.41 -5.24 -7.16
Peroxisomes -1.93 -9.04 0.71 6.45 10.51 14.58 -4.03 -0.46 -4.35 1.07 5.99 -7.47 5.43 0.91 11.39
Vacuole 1.76 1.20 -2.55 -4.23 -3.74 -0.02 -1.29 -2.02 -2.01 -1.86 1.38 -1.03 -4.48 -4.64 -4.08
Vacuole Periphery 1.46 1.65 0.43 -3.97 -4.70 -1.04 -1.78 -4.58 -4.09 -4.08 0.89 0.45 -3.97 -4.43 -4.25
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Constituent of 66S pre-ribosomal particles; required for large (60S) ribosomal subunit biogenesis; acts as part of a Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription and has a role in bigenesis of the large ribosomal subunit; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
Localization
Cell Percentages nucleolus (80%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Mak21

Mak21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mak21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available