Standard name
Human Ortholog
Description Protein that interacts with Arp2/3 complex; interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0 0.05 0 0 0 0 0 0
Cytoplasm 0.98 0.96 0.93 0.97 0.9 0.72 – 0.57 0.51 0.5 0.52 0.53 1.0 0.95 0.98 0.96 0.93 0.93 0.95 0.94 0.92 0.74 0.8 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.11 0.06 0.26 – 0.24 0.43 0.33 0.45 0.38 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.12 – 0.23 0.21 0.21 0.09 0.08 0 0 0 0 0.08 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 1 – 7 2 8 5 9 0 0 0 1 0 1 1 2 2 2 3 6
Bud 1 0 3 1 3 14 – 6 10 11 8 12 0 0 1 1 0 0 0 0 0 2 3 3
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 1 0 0 2 2 – 1 8 4 5 13 0 0 1 10 6 7 0 0 0 0 0 0
Cytoplasm 382 134 96 112 283 200 – 298 189 198 154 238 86 153 197 134 169 130 369 133 100 60 187 188
Endoplasmic Reticulum 1 2 0 1 7 6 – 17 6 12 11 26 1 0 2 4 5 1 0 0 0 0 6 4
Endosome 0 0 0 0 5 4 – 7 5 9 2 4 0 0 0 0 2 2 4 0 0 4 6 4
Golgi 0 0 0 0 0 0 – 0 0 1 0 0 0 1 0 0 0 0 4 0 0 2 3 4
Mitochondria 6 0 8 13 20 71 – 127 159 130 132 173 0 2 0 2 1 0 0 0 2 2 2 2
Nucleus 1 0 1 0 2 2 – 14 7 14 8 12 0 4 0 0 4 0 0 0 0 0 2 3
Nuclear Periphery 0 0 1 0 0 0 – 6 2 3 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 – 4 3 3 0 0 0 0 0 0 2 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 – 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 1
Vac/Vac Membrane 3 4 0 2 7 32 – 119 77 81 26 35 0 3 3 1 14 14 2 1 1 1 5 4
Unique Cell Count 388 139 103 116 315 277 522 369 394 295 450 86 161 202 139 182 140 387 142 109 82 233 233
Labelled Cell Count 397 141 109 129 334 332 607 469 474 352 524 87 163 204 153 203 155 387 142 109 82 233 233


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 9.6 4.6 5.5 6.2 5.7 – 6.3 6.3 6.7 6.7 7.1 7.5 8.2 8.6 13.3 12.5 12.4 6.8 7.8 7.8
Std Deviation (1e-4) 1.0 1.4 0.8 1.1 1.2 1.3 – 1.4 1.6 1.5 2.0 1.9 1.2 1.8 1.6 3.0 2.7 2.6 1.4 2.1 1.9
Intensity Change (Log2) – – – 0.24 0.43 0.3 – 0.45 0.44 0.52 0.54 0.61 0.69 0.82 0.89 1.52 1.43 1.41 0.55 0.75 0.75


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 -1.0 -4.4 – -6.9 -7.7 -7.9 -7.4 -7.5 2.5 0.6 1.8 1.1 -0.1 -0.1
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.9 -0.5 3.8 – 3.7 6.6 5.1 6.8 6.0 0 0 0 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.6 – 5.4 5.1 5.0 3.1 2.9 0 0 0 0 2.9 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9412 5.3206 4.4594 4.2505 3.6745 4.2727 2.9725 4.0487 3.5902 3.2948 3.5494 3.5597 4.5803 5.9171 5.4638 5.4371 4.4321 5.2671
Actin 0.0114 0.0005 0.0027 0.0183 0.0187 0.0018 0.0371 0.0011 0.0194 0.015 0.0388 0.0058 0.0128 0.0012 0.0074 0.0002 0.0045 0.0005
Bud 0.0007 0.0002 0.0011 0.0004 0.0007 0.0001 0.0011 0.0007 0.0013 0.0023 0.0006 0.0002 0.0002 0.0001 0.0003 0.0003 0.0002 0.0001
Bud Neck 0.0036 0.0003 0.0008 0.0007 0.0009 0.0035 0.0052 0.0005 0.0018 0.0014 0.001 0.0018 0.0007 0.0002 0.0008 0.0003 0.0012 0.0054
Bud Periphery 0.001 0 0.0008 0.0003 0.0009 0.0001 0.0015 0.0003 0.0012 0.0022 0.0006 0.0002 0.0001 0 0.0001 0.0001 0.0001 0
Bud Site 0.0017 0.0006 0.0009 0.0019 0.0029 0.0001 0.0098 0.0035 0.0114 0.0073 0.0098 0.0003 0.0004 0.001 0.0005 0.0001 0.0003 0.0001
Cell Periphery 0.0001 0 0.0001 0.0001 0.0002 0 0.0004 0.0001 0.0003 0.0002 0.0001 0 0.0001 0.0001 0 0 0 0
Cytoplasm 0.8538 0.9775 0.9028 0.8506 0.7705 0.9237 0.6888 0.9134 0.875 0.8545 0.7591 0.8829 0.8724 0.9623 0.9321 0.9759 0.9353 0.9517
Cytoplasmic Foci 0.0304 0.0029 0.0158 0.0163 0.0288 0.0121 0.0574 0.0138 0.0161 0.0184 0.0411 0.021 0.0123 0.0039 0.0132 0.0019 0.0147 0.0066
Eisosomes 0.0003 0 0.0001 0.0001 0.0003 0 0.0001 0 0.0003 0.0001 0.0002 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0069 0.0029 0.003 0.0039 0.0042 0.0039 0.0045 0.0025 0.0027 0.0039 0.0031 0.0021 0.006 0.0018 0.0021 0.0013 0.002 0.0022
Endosome 0.0123 0.0012 0.0087 0.0107 0.0405 0.0083 0.0528 0.0057 0.0116 0.0362 0.0188 0.0135 0.0091 0.001 0.0034 0.001 0.0033 0.0014
Golgi 0.0031 0.0001 0.0018 0.015 0.0123 0.0009 0.0104 0.0006 0.005 0.0068 0.0156 0.005 0.0017 0.0002 0.0008 0 0.0005 0.0001
Lipid Particles 0.0049 0 0.0021 0.0041 0.0303 0.0017 0.0169 0.0002 0.005 0.0049 0.0162 0.005 0.0023 0 0.0001 0 0 0
Mitochondria 0.0016 0.0001 0.0217 0.0024 0.0509 0.0016 0.0056 0.0004 0.0076 0.0058 0.0256 0.0027 0.0009 0.0001 0.0006 0.0002 0.0006 0.0002
None 0.0366 0.005 0.0219 0.0437 0.0069 0.0149 0.0363 0.0419 0.0183 0.0109 0.0078 0.0266 0.0663 0.0239 0.0299 0.0095 0.0283 0.0213
Nuclear Periphery 0.0031 0.0015 0.002 0.0034 0.005 0.0025 0.0143 0.0013 0.0019 0.0055 0.0028 0.0032 0.0046 0.0005 0.0011 0.001 0.0008 0.0009
Nucleolus 0.0005 0 0.0003 0.0003 0.0006 0.0001 0.0015 0.0002 0.0003 0.0006 0.0004 0.0001 0.0002 0 0 0 0 0
Nucleus 0.0134 0.0061 0.0066 0.0125 0.0107 0.0156 0.0123 0.009 0.0065 0.0106 0.0052 0.0115 0.0042 0.0029 0.0041 0.007 0.0042 0.0077
Peroxisomes 0.0081 0.0001 0.0021 0.0098 0.0052 0.0057 0.0201 0.001 0.0089 0.003 0.0484 0.0115 0.0014 0.0001 0.0017 0 0.0019 0.0004
Punctate Nuclear 0.004 0.0004 0.0016 0.0039 0.0029 0.002 0.0139 0.0028 0.0029 0.0057 0.0035 0.0057 0.0034 0.0003 0.001 0.0003 0.0015 0.0008
Vacuole 0.0021 0.0006 0.0019 0.0013 0.0041 0.001 0.0073 0.0007 0.0018 0.0035 0.0009 0.0007 0.0006 0.0002 0.0004 0.0008 0.0003 0.0003
Vacuole Periphery 0.0004 0.0001 0.0012 0.0003 0.0025 0.0003 0.0027 0.0002 0.0009 0.0013 0.0004 0.0004 0.0003 0 0.0001 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 34.3281 35.0712 34.1569 39.8728 19.0748 26.9572 27.1494 20.1938 38.6503 34.9368
Translational Efficiency 1.8311 1.497 1.5609 1.586 3.6458 2.7912 2.056 2.6955 1.9643 2.0779

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1045 749 1593 1222 1731 90 236 1173 2776 839 1829 2395

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 835.30 941.43 1690.93 1027.34 830.02 966.47 1145.03 998.00 832.01 944.12 1620.49 1012.97
Standard Deviation 110.42 139.96 268.82 143.91 120.15 140.97 214.97 136.11 116.61 140.28 319.99 140.91
Intensity Change Log 2 — 0.172559 1.017451 0.298547 — 0.219579 0.464167 0.265894 — 0.196186 0.768038 0.282365

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000445 0.002004 0.002076 0.007834 0.000458 0.001327 0.001936 0.010783 0.000453 0.001931 0.002058 0.009278
Bud Neck 0.005440 0.007408 0.005068 0.006921 0.006876 0.007995 0.004724 0.009222 0.006336 0.007471 0.005023 0.008048
Bud Site 0.010313 0.020096 0.014357 0.045110 0.009141 0.022391 0.041110 0.054145 0.009582 0.020342 0.017809 0.049535
Cell Periphery 0.000166 0.000109 0.000272 0.000372 0.000109 0.000309 0.000304 0.000209 0.000130 0.000131 0.000276 0.000292
Cytoplasm 0.663019* 0.575796* 0.612472* 0.479115* 0.592047* 0.411799* 0.499878* 0.524431* 0.618764* 0.558204* 0.597944* 0.501309*
Cytoplasmic Foci 0.168620 0.187895* 0.016196 0.038077 0.181025* 0.277859* 0.022492 0.038961 0.176355 0.197546* 0.017008 0.038510
Eisosomes 0.000069 0.000060 0.000008 0.000073 0.000093 0.000195 0.000053 0.000071 0.000084 0.000075 0.000014 0.000072
Endoplasmic Reticulum 0.001550 0.000970 0.000832 0.004529 0.001547 0.003144 0.004076 0.001648 0.001548 0.001203 0.001250 0.003118
Endosome 0.008633 0.013638 0.001039 0.013312 0.009764 0.030634 0.003699 0.007429 0.009338 0.015461 0.001382 0.010431
Golgi 0.002016 0.004028 0.000666 0.014466 0.002039 0.008068 0.000301 0.006438 0.002030 0.004462 0.000619 0.010534
Lipid Particles 0.002868 0.001894 0.002103 0.002325 0.004581 0.006332 0.014758 0.001744 0.003936 0.002370 0.003735 0.002041
Mitochondria 0.001258 0.002871 0.000447 0.004921 0.002743 0.011481 0.001772 0.006353 0.002184 0.003794 0.000618 0.005622
Mitotic Spindle 0.001419 0.020919 0.016118 0.076334 0.004682 0.001021 0.005361 0.036014 0.003454 0.018785 0.014730 0.056586
None 0.004828 0.003120 0.006937 0.002390 0.001846 0.001080 0.005436 0.002611 0.002968 0.002901 0.006744 0.002498
Nuclear Periphery 0.000180 0.000192 0.000480 0.000682 0.000301 0.002316 0.000689 0.000687 0.000255 0.000419 0.000507 0.000685
Nuclear Periphery Foci 0.001858 0.001349 0.003454 0.002378 0.000988 0.006916 0.012372 0.001620 0.001316 0.001946 0.004605 0.002007
Nucleolus 0.000493 0.000516 0.000209 0.000221 0.000573 0.000871 0.000224 0.000361 0.000543 0.000554 0.000211 0.000290
Nucleus 0.078835 0.051623 0.295020* 0.107261 0.099017 0.057254 0.206433* 0.081224 0.091419 0.052227 0.283589* 0.094509
Peroxisomes 0.003427 0.006097 0.000324 0.002465 0.003340 0.012837 0.002142 0.007667 0.003373 0.006820 0.000559 0.005013
Vacuole 0.042903 0.098379 0.021762 0.187256 0.077878 0.131648 0.172007 0.200534* 0.064712 0.101948 0.041149 0.193759
Vacuole Periphery 0.001660 0.001037 0.000161 0.003958 0.000954 0.004522 0.000231 0.007847 0.001220 0.001410 0.000170 0.005863

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.78 -5.87 -3.17 -1.28 -0.71 -2.46 -5.56 -3.19 -2.39 -1.64 -1.89 -6.51 -4.48 -1.93 -1.38
Bud Neck -1.54 -4.97 -0.28 1.53 6.00 -0.48 0.70 0.73 0.74 -0.06 -0.96 -4.65 0.70 1.38 5.26
Bud Site -3.66 -5.03 -5.78 -1.92 -1.71 -2.78 -3.27 -7.01 -0.74 0.18 -4.93 -7.01 -9.25 -2.49 -2.00
Cell Periphery 4.73 -11.53 -1.30 -3.06 6.04 -4.94 -8.93 -4.26 3.77 7.09 0.40 -15.04 -2.79 -2.86 9.73
Cytoplasm 7.46 13.78 23.66 12.42 13.03 6.21 10.77 14.72 -0.88 -2.32 5.99 13.69 26.10 11.41 11.83
Cytoplasmic Foci -1.48 26.54 25.09 21.85 -3.06 -4.35 31.61 32.52 11.34 -2.62 -2.08 43.52 41.29 24.10 -5.20
Eisosomes 1.74 11.15 0.19 -1.75 -13.51 -4.17 3.43 4.02 5.35 0.13 1.80 15.07 3.49 1.09 -17.31
Endoplasmic Reticulum 2.19 1.50 -3.57 -5.68 -5.38 -1.24 -7.16 -0.20 1.22 7.64 1.49 -0.58 -3.26 -4.30 -3.30
Endosome -3.34 10.22 3.63 5.95 -3.81 -4.22 8.19 9.05 5.54 0.74 -4.49 16.33 8.23 8.78 -4.64
Golgi -2.37 5.58 -1.56 -0.17 -2.69 -1.91 7.45 0.13 1.88 -1.87 -3.03 7.83 -1.21 1.18 -3.07
Lipid Particles 3.12 -3.84 1.37 -2.24 5.63 -0.79 -0.75 4.26 2.19 1.50 3.18 -1.33 4.26 0.93 4.79
Mitochondria -1.11 1.51 -0.93 0.68 -3.30 -0.86 2.38 0.53 0.91 -1.61 -0.89 3.41 0.29 1.02 -3.34
Mitotic Spindle -4.37 -8.78 -7.90 -1.86 -3.25 3.07 -0.88 -5.20 -6.79 -3.68 -3.81 -7.42 -9.13 -1.67 -3.38
None 1.91 -1.54 3.89 0.64 10.65 1.73 -2.18 -1.67 -3.20 1.35 0.33 -6.87 1.66 0.39 10.71
Nuclear Periphery -1.32 -18.14 -16.52 -16.08 -5.69 -1.01 -9.64 -7.84 0.70 1.93 -0.83 -16.84 -15.91 -2.99 -3.65
Nuclear Periphery Foci 2.13 -8.70 -1.71 -3.75 7.54 -1.61 -3.60 -4.50 1.43 3.39 -1.57 -9.25 -4.82 0.08 7.96
Nucleolus -0.80 6.95 2.01 2.00 -0.54 -1.12 4.33 3.89 2.25 -0.73 -0.54 6.00 3.98 3.03 -0.57
Nucleus 6.41 -39.80 -10.68 -15.67 27.12 3.79 -8.58 -2.15 -4.59 7.70 10.87 -39.57 -8.58 -17.06 31.97
Peroxisomes -2.35 5.85 3.47 4.74 -4.34 -2.44 3.74 0.05 2.40 -2.21 -3.43 10.51 2.09 4.28 -4.46
Vacuole -11.66 -4.22 -34.89 -22.42 -32.78 -3.43 -12.07 -29.99 -11.42 -2.53 -8.76 -5.02 -44.68 -28.05 -36.85
Vacuole Periphery 1.54 3.44 0.86 -0.40 -2.31 -1.55 4.89 -1.47 0.85 -2.31 -0.35 5.49 -0.94 -0.69 -3.09
Standard name
Human Ortholog
Description Protein that interacts with Arp2/3 complex; interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss
Localization
Cell Percentages cytoplasm (79%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Aim7

Aim7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aim7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available