Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.07 0.06 0.06 0.06 0.08 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0
Cytoplasm 0.94 0.86 0.94 0.79 0.8 0.69 0.62 0.49 0.53 0.42 0.37 0.38 0.85 0.92 0.94 0.78 0.87 0.84 0.73 0.75 0.62 0.76 0.76 0.81
Endoplasmic Reticulum 0.05 0.06 0.06 0.09 0.13 0.1 0.1 0.14 0.08 0.05 0.08 0.07 0.16 0.09 0.06 0 0 0 0.13 0.07 0.14 0.11 0.13 0.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0.09 0.12 0 0 0 0.19 0.29 0.36 0.45 0.46 0.63 0.46 0 0 0 0 0 0 0 0 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.06 0 0 0.06 0.09 0.06 0.12 0 0.14 0 0 0 0.16 0 0.09 0 0.09 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 1 12 15 13 19 25 15 9 12 20 7 2 4 3 2 3 0 0 0 1 4 1
Bud 0 0 0 1 0 1 4 8 7 6 9 7 0 0 0 0 0 1 0 0 0 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 0 4 7 3 13 11 14 11 13 10 5 7 13 9 12 8 0 0 0 1 0 0
Cytoplasm 97 71 32 136 216 149 185 148 202 94 106 117 327 476 492 159 109 163 87 85 46 170 362 340
Endoplasmic Reticulum 5 5 2 15 35 22 31 43 31 12 24 20 62 48 33 7 3 7 15 8 10 25 60 41
Endosome 1 0 0 3 3 1 0 5 4 2 1 4 0 1 1 1 0 0 1 0 2 2 5 3
Golgi 0 0 0 3 8 10 4 0 5 4 0 7 1 2 0 9 2 10 3 3 2 1 10 7
Mitochondria 9 10 0 3 10 42 88 108 172 105 182 139 0 0 0 4 5 7 4 4 6 8 13 5
Nucleus 0 0 0 0 0 0 2 0 0 0 0 1 0 1 4 2 0 0 0 0 0 0 1 0
Nuclear Periphery 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0
Vac/Vac Membrane 0 3 0 11 7 4 17 27 24 26 7 44 8 5 5 32 3 18 3 10 5 8 11 9
Unique Cell Count 103 83 34 172 271 217 299 303 384 226 290 304 383 520 525 203 125 194 120 115 76 223 478 419
Labelled Cell Count 113 92 35 189 301 245 363 375 474 271 355 371 410 542 552 227 136 217 120 115 76 223 478 419


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.0 9.3 10.6 11.0 10.3 9.0 8.4 8.6 8.0 7.6 7.1 7.4 12.6 13.1 12.9 14.1 15.3 15.7 11.7 12.2 12.5
Std Deviation (1e-4) 1.5 2.8 1.4 1.7 1.5 1.6 1.7 1.7 1.7 1.5 1.4 1.5 1.7 2.0 2.0 3.1 3.2 2.8 1.8 1.9 1.9
Intensity Change (Log2) – – – 0.05 -0.05 -0.24 -0.34 -0.31 -0.42 -0.48 -0.59 -0.52 0.24 0.3 0.28 0.4 0.52 0.56 0.14 0.2 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.1 -2.0 -3.1 -3.7 -5.0 -4.7 -5.7 -6.4 -6.2 -1.4 -0.5 -0.1 -2.2 -1.1 -1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 1.6 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.8 3.7 4.2 5.1 5.1 7.0 5.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.1661 6.7768 5.6104 5.1447 5.0012 5.3822 3.9353 7.2797 6.2894 4.873 4.4356 6.3865 4.5841 7.0579 6.0253 5.6797 5.7039 6.3932
Actin 0.0092 0.0058 0.0169 0.0321 0.0019 0.0116 0.0126 0.0102 0.0063 0.0015 0.0061 0.0031 0.0229 0.0012 0.0099 0.0022 0.0006 0.0064
Bud 0.0012 0.0009 0.0029 0.0072 0.0009 0.0007 0.0009 0.001 0.0013 0.0005 0.0004 0.0009 0.0012 0.0029 0.0011 0.0018 0.0005 0.001
Bud Neck 0.0008 0.0004 0.0052 0.0012 0.0006 0.001 0.0006 0.0004 0.0006 0.0005 0.0006 0.0011 0.0018 0.0004 0.0005 0.0008 0.0005 0.0016
Bud Periphery 0.0008 0.0003 0.0015 0.0113 0.0011 0.0008 0.0005 0.0003 0.0005 0.0003 0.0002 0.0007 0.0011 0.001 0.0005 0.002 0.0002 0.0009
Bud Site 0.0027 0.0024 0.0204 0.0025 0.0012 0.0004 0.0029 0.0038 0.0041 0.0006 0.0004 0.0004 0.0041 0.0052 0.0018 0.0006 0.0003 0.0003
Cell Periphery 0.0007 0.0002 0.0004 0.0004 0.0002 0.0003 0.0007 0.0002 0.0002 0 0.0001 0.0002 0.0004 0.0001 0.0002 0.0005 0 0.0003
Cytoplasm 0.4294 0.6869 0.4796 0.3782 0.2648 0.4834 0.5303 0.7135 0.6033 0.3162 0.4666 0.5484 0.3881 0.6888 0.4798 0.3712 0.4785 0.4301
Cytoplasmic Foci 0.0728 0.0567 0.0635 0.0589 0.0642 0.0713 0.0843 0.0559 0.0605 0.0531 0.0567 0.0543 0.0885 0.06 0.0673 0.0598 0.0487 0.0644
Eisosomes 0.0004 0 0.0004 0.0001 0 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0202 0.0064 0.0131 0.0319 0.0427 0.0177 0.0222 0.0094 0.0071 0.0027 0.0038 0.0201 0.0499 0.0062 0.0097 0.0216 0.0073 0.0244
Endosome 0.2842 0.1848 0.2542 0.3094 0.481 0.2925 0.2205 0.1539 0.2317 0.4829 0.3153 0.2363 0.2968 0.1807 0.3275 0.3845 0.3779 0.3239
Golgi 0.0782 0.0257 0.0584 0.0327 0.0444 0.0444 0.0533 0.0311 0.046 0.0446 0.0899 0.063 0.0636 0.0347 0.0495 0.0552 0.0463 0.0999
Lipid Particles 0.0112 0.0002 0.0055 0.0145 0.0048 0.0056 0.0144 0.0003 0.0031 0.0248 0.0123 0.0126 0.007 0.0002 0.004 0.0004 0.009 0.0005
Mitochondria 0.039 0.0098 0.0293 0.009 0.0228 0.0266 0.0132 0.0068 0.0135 0.0128 0.022 0.04 0.0245 0.0066 0.0139 0.0408 0.0067 0.0292
None 0.0041 0.0017 0.0205 0.0026 0.0008 0.0103 0.0043 0.0018 0.0014 0.0007 0.0007 0.0008 0.0037 0.0015 0.0009 0.0007 0.0004 0.0014
Nuclear Periphery 0.0069 0.001 0.0014 0.0082 0.0144 0.0102 0.0066 0.0012 0.001 0.0018 0.0017 0.0006 0.0139 0.0006 0.0025 0.0027 0.0017 0.0015
Nucleolus 0.0005 0 0.0005 0.0001 0.0003 0.0001 0.0001 0.0001 0 0.0002 0 0.0002 0.0002 0 0.0001 0 0.0001 0.0001
Nucleus 0.0057 0.0009 0.0009 0.0017 0.0023 0.0027 0.0022 0.001 0.0006 0.001 0.0006 0.0004 0.0018 0.0006 0.0008 0.0009 0.0007 0.0006
Peroxisomes 0.0075 0.0003 0.0042 0.0186 0.001 0.0023 0.0123 0.0004 0.002 0.0078 0.0013 0.0052 0.0055 0.0003 0.0017 0.0003 0.0006 0.0004
Punctate Nuclear 0.0021 0.0002 0.0008 0.0028 0.001 0.002 0.0016 0.0008 0.0002 0.0011 0.0003 0.0006 0.0046 0.0001 0.0005 0.0001 0.0002 0.0006
Vacuole 0.015 0.0109 0.0129 0.0611 0.0303 0.0096 0.0119 0.0051 0.0104 0.0278 0.0138 0.0058 0.014 0.0057 0.0181 0.0337 0.0109 0.0065
Vacuole Periphery 0.0072 0.0044 0.0074 0.0154 0.0194 0.0062 0.0044 0.0028 0.0061 0.0192 0.0073 0.0051 0.0062 0.0033 0.0096 0.0202 0.009 0.0062

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.1483 28.5446 20.3969 22.0469 22.2335 29.5497 23.5167 23.0869 28.9981 22.2578
Translational Efficiency 2.0967 1.4336 1.6589 2.2264 1.6317 1.3465 1.8258 1.6258 1.4141 1.7689

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
270 669 2599 167 1795 1617 257 1203 2065 2286 2856 1370

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1103.14 1281.58 1123.40 1414.86 1086.85 1156.11 1458.26 1367.85 1088.98 1192.83 1153.53 1373.58
Standard Deviation 156.22 186.71 154.20 178.41 174.44 180.74 174.90 198.93 172.26 191.23 183.23 197.15
Intensity Change Log 2 — 0.216308 0.026256 0.359043 — 0.089126 0.424095 0.331757 — 0.154590 0.237375 0.345566

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000368 0.000369 0.000968 0.003479 0.000198 0.000397 0.002394 0.001427 0.000220 0.000389 0.001096 0.001678
Bud Neck 0.000800 0.000303 0.000681 0.003264 0.000309 0.000330 0.004045 0.001762 0.000373 0.000323 0.000984 0.001945
Bud Site 0.058036 0.038443 0.038048 0.149220* 0.026906 0.036830 0.088302 0.066893 0.030976 0.037302 0.042570 0.076928
Cell Periphery 0.000704 0.000478 0.001075 0.008545 0.000444 0.000386 0.001037 0.000867 0.000478 0.000413 0.001072 0.001803
Cytoplasm 0.213456* 0.211303* 0.300910* 0.359504* 0.295044* 0.276291* 0.277908* 0.475373* 0.284376* 0.257272* 0.298840* 0.461249*
Cytoplasmic Foci 0.508842* 0.485493* 0.250734* 0.189279* 0.420314* 0.440319* 0.295225* 0.189168* 0.431889* 0.453539* 0.254738* 0.189181*
Eisosomes 0.000020 0.000012 0.000034 0.000035 0.000014 0.000017 0.000022 0.000013 0.000014 0.000015 0.000033 0.000016
Endoplasmic Reticulum 0.006744 0.007362 0.006198 0.009050 0.008220 0.005924 0.008301 0.002257 0.008027 0.006345 0.006387 0.003085
Endosome 0.040054 0.032094 0.046019 0.042782 0.025662 0.028822 0.076835 0.035378 0.027544 0.029779 0.048792 0.036281
Golgi 0.093601 0.157317* 0.125789* 0.082138 0.089066 0.132898* 0.065337 0.066125 0.089659 0.140044* 0.120349* 0.068077
Lipid Particles 0.000879 0.001327 0.005463 0.019447 0.000889 0.001596 0.011317 0.006380 0.000888 0.001518 0.005989 0.007972
Mitochondria 0.003608 0.002319 0.005002 0.000083 0.006401 0.004840 0.000042 0.001253 0.006036 0.004102 0.004555 0.001110
Mitotic Spindle 0.000890 0.004575 0.001843 0.006440 0.001137 0.002387 0.014254 0.007350 0.001105 0.003027 0.002960 0.007239
None 0.000069 0.000109 0.000102 0.000057 0.000804 0.000187 0.000036 0.000058 0.000708 0.000164 0.000096 0.000058
Nuclear Periphery 0.000017 0.000023 0.000052 0.000036 0.000035 0.000032 0.000122 0.000226 0.000032 0.000029 0.000058 0.000203
Nuclear Periphery Foci 0.000243 0.000415 0.000614 0.002858 0.000364 0.000710 0.004686 0.001874 0.000348 0.000624 0.000981 0.001994
Nucleolus 0.000013 0.000010 0.000061 0.000046 0.000052 0.000041 0.000037 0.000101 0.000047 0.000032 0.000059 0.000094
Nucleus 0.000256 0.000157 0.000563 0.000752 0.000689 0.000308 0.001187 0.001356 0.000632 0.000264 0.000619 0.001282
Peroxisomes 0.000515 0.000476 0.000917 0.002639 0.000281 0.000421 0.001835 0.002174 0.000312 0.000437 0.001000 0.002230
Vacuole 0.018015 0.013435 0.111016 0.100071 0.029550 0.016204 0.124904 0.107698 0.028042 0.015394 0.112266 0.106768
Vacuole Periphery 0.052871 0.043978 0.103909* 0.020275 0.093623 0.051060 0.022175 0.032269 0.088294 0.048988 0.096555 0.030807

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.02 -2.21 -5.49 -5.50 -4.02 -4.37 -8.24 -3.14 -2.63 1.91 -4.49 -3.60 -4.12 -3.64 -1.40
Bud Neck 3.70 0.84 -6.23 -7.87 -6.84 -0.75 -10.97 -14.05 -13.86 6.11 1.66 -10.23 -15.21 -15.85 -8.82
Bud Site 3.79 4.22 -6.81 -8.68 -8.83 -5.32 -9.83 -13.70 -9.60 3.40 -3.67 -6.67 -14.60 -12.37 -10.66
Cell Periphery 2.94 -3.48 -3.36 -3.46 -3.19 1.23 -4.02 -4.78 -5.82 1.14 1.59 -7.16 -4.34 -4.58 -2.28
Cytoplasm 0.13 -5.81 -5.68 -6.28 -2.67 1.99 0.72 -16.46 -18.11 -10.77 3.30 -1.91 -17.39 -20.66 -16.65
Cytoplasmic Foci 1.48 18.50 17.30 19.59 4.66 -2.54 9.86 31.35 34.47 8.27 -3.10 27.85 35.25 41.10 11.75
Eisosomes 2.44 -3.24 -2.26 -3.65 -0.14 -2.35 -3.63 0.47 2.55 3.76 -0.89 -5.82 -1.00 -0.25 5.22
Endoplasmic Reticulum -0.31 0.37 -0.89 -0.61 -1.30 2.40 0.08 7.77 5.06 3.51 1.87 2.09 6.75 4.45 5.46
Endosome 2.32 -1.83 -0.51 -2.67 0.95 -2.41 -10.29 -5.44 -3.25 8.26 -1.90 -15.74 -5.12 -3.64 7.89
Golgi -8.88 -5.42 1.45 8.76 5.81 -11.63 4.90 7.10 16.44 0.04 -15.25 -9.43 7.20 19.80 14.82
Lipid Particles -1.85 -8.72 -5.66 -5.52 -4.24 -1.49 -6.30 -9.32 -6.48 2.68 -1.83 -10.23 -10.70 -8.78 -2.62
Mitochondria 0.66 -0.71 1.86 4.71 14.22 1.66 8.92 6.41 5.03 -2.98 2.39 2.03 6.64 5.36 7.50
Mitotic Spindle -2.51 -2.82 -2.28 -0.70 -1.88 -2.98 -4.96 -4.40 -3.48 2.19 -3.66 -4.78 -4.80 -3.21 -3.29
None -1.56 -2.37 0.75 2.09 3.64 2.15 2.67 2.59 5.89 -2.98 2.18 2.45 2.59 6.19 4.87
Nuclear Periphery -0.87 -4.78 -3.75 -1.89 2.07 0.34 -4.70 -1.72 -1.75 -0.91 0.50 -3.85 -1.75 -1.78 -1.48
Nuclear Periphery Foci -2.04 -4.56 -4.94 -4.58 -4.20 -1.64 -7.60 -4.00 -2.90 4.20 -1.73 -5.30 -4.83 -3.89 -2.98
Nucleolus 1.23 -15.67 -3.67 -3.95 1.56 0.44 0.69 -1.95 -2.46 -2.90 0.71 -0.60 -2.10 -2.91 -2.14
Nucleus 3.35 -2.16 -5.23 -6.33 -1.25 2.25 -2.58 -3.08 -4.17 -1.90 2.50 0.00 -3.25 -4.43 -3.29
Peroxisomes 0.28 -4.89 -6.38 -6.17 -5.18 -3.78 -7.26 -10.46 -9.55 -1.21 -2.71 -11.21 -11.72 -10.63 -7.09
Vacuole 1.46 -22.13 -6.74 -7.25 0.77 6.57 -9.28 -17.50 -21.04 1.19 7.15 -25.04 -18.78 -22.47 -0.02
Vacuole Periphery 1.56 -9.08 5.92 6.30 22.84* 10.95 14.46 16.64 7.03 -2.20 11.62 -2.09 17.39 8.00 22.30*
Standard name
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-5

Dos2

Dos2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dos2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available