Standard name
Human Ortholog
Description Ubiquitin hydrolase; deubiquitinates intralumenal vesicle (ILVs) cargo proteins; required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins destined for the vacuole; DOA4 has a paralog, UBP5, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.06 0 0 0 0 0 0
Bud 0 – 0 0.09 0.05 0.08 0.1 0.14 0.12 0.13 0.11 0.1 0 0 0.09 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.32 – 0.61 0.25 0.11 0.12 0.2 0.17 0.19 0.19 0.13 0.15 0.49 0.4 0.64 0.54 0.46 0.48 0.09 0.2 0.26 0.39 0.14 0.08
Endoplasmic Reticulum 0.07 – 0.13 0.09 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0
Endosome 0.54 – 0 0.09 0.31 0.09 0.15 0.14 0.05 0 0 0 0.44 0.55 0.22 0.3 0.38 0.37 0.65 0.44 0.39 0.32 0.53 0.64
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0.26 0.46 0.3 0.59 0.42 0.41 0.65 0.63 0.71 0.7 0 0 0.08 0 0 0 0.1 0.15 0.14 0.06 0.08 0.08
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0.05 0.07 0 0.11 0.07 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.15 – 0.05 0.23 0.35 0.22 0.27 0.31 0.18 0.24 0.2 0.19 0.07 0.06 0 0.12 0.18 0.13 0 0 0.05 0 0.05 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0 0.11 0.09 0.12 0.09 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 9 – 1 4 0 2 2 2 0 2 0 1 1 3 3 31 14 28 4 0 3 1 6 7
Bud 2 – 4 15 12 21 41 64 33 53 29 34 2 10 26 3 1 7 0 0 0 0 1 3
Bud Neck 0 – 2 0 6 1 3 6 3 3 1 1 0 0 0 1 0 2 1 0 1 1 2 1
Bud Site 0 – 0 0 0 0 0 8 5 9 5 8 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 1 1 0 1 0 0 0 0 0 4 1 1 0 0 0 0 0 0
Cytoplasm 63 – 83 42 29 31 78 77 52 78 35 55 67 106 189 213 124 212 21 6 47 55 40 27
Endoplasmic Reticulum 13 – 17 15 2 11 6 1 2 3 2 4 2 7 6 24 10 28 2 1 1 1 3 4
Endosome 107 – 0 15 81 22 61 63 14 16 7 15 61 144 66 120 104 165 166 13 71 44 151 221
Golgi 2 – 0 0 1 0 0 0 0 0 0 0 0 1 0 1 1 0 6 0 0 0 2 5
Mitochondria 6 – 35 79 80 151 164 182 173 265 186 249 2 1 24 7 6 6 26 4 26 8 22 27
Nucleus 0 – 1 0 1 1 1 2 0 0 1 0 0 2 0 0 1 1 0 0 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 1 2
Nucleolus 0 – 0 0 2 5 3 3 2 0 0 0 1 1 1 0 0 0 0 0 0 0 2 0
Peroxisomes 0 – 0 8 18 9 45 29 13 27 8 11 1 4 1 0 8 0 2 0 0 0 1 2
SpindlePole 30 – 7 39 94 57 107 139 47 101 52 67 9 16 5 46 48 59 11 0 9 6 13 9
Vac/Vac Membrane 3 – 0 1 7 4 8 4 3 3 5 7 6 6 10 15 23 20 9 3 16 16 24 28
Unique Cell Count 199 135 170 265 255 395 442 267 418 263 357 138 262 294 396 272 444 258 31 184 140 283 347
Labelled Cell Count 235 150 218 333 315 520 581 347 562 331 452 152 301 331 465 341 530 258 31 184 140 283 347


Ambiguous, Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 – 3.6 3.7 3.9 3.6 3.4 3.6 3.3 3.3 3.3 3.1 5.5 5.5 4.8 6.6 7.3 7.6 5.1 5.0 5.6
Std Deviation (1e-4) 0.6 – 0.4 1.2 0.9 0.7 0.7 0.7 0.9 1.0 0.9 0.9 1.4 1.3 1.0 0.9 1.5 1.3 1.5 1.7 1.5
Intensity Change (Log2) – – – 0.04 0.1 -0.01 -0.07 -0.02 -0.14 -0.15 -0.12 -0.2 0.6 0.61 0.42 0.86 1.01 1.08 0.5 0.47 0.64

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0 2.6
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -6.5 -10.6 -10.2 -9.1 -10.0 -8.4 -9.5 -10.0 -10.2 -2.1 -4.0 0.6 -1.6 -3.0 -2.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 -3.6 -3.9 0 -2.5 -3.4 -2.4
Endosome 0 0 0 0 0 0 0 0 0 8.8 10.8 0 7.3 8.3 8.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -5.9 -8.4 0 -9.0 -7.5 -9.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0.5 0.4 0 2.2 3.5 2.6
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 3.5 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5571 1.6804 1.1872 1.1345 1.2107 1.2133 1.146 1.9058 1.6271 1.6328 1.557 1.5821 -0.2337 0.287 0.2592 0.3331 0.152 0.1785
Actin 0.0381 0.005 0.0085 0.0017 0.0289 0.0016 0.0229 0.0011 0.022 0.0134 0.03 0.0076 0.0044 0.0003 0.0045 0.0001 0.0074 0.0014
Bud 0.0075 0.0082 0.0004 0.0002 0.0008 0.0007 0.0009 0.0001 0.0009 0.0003 0.0009 0.0003 0.0001 0.0003 0.0007 0.0001 0.0001 0.0001
Bud Neck 0.0042 0.0004 0.001 0.0005 0.0017 0.0037 0.006 0.0002 0.0004 0.0002 0.0008 0.001 0.0003 0.0005 0.0004 0.0001 0.0006 0.0005
Bud Periphery 0.0043 0.0047 0.0005 0.0002 0.0015 0.0012 0.0016 0.0001 0.0012 0.0002 0.002 0.0003 0.0001 0.0003 0.0028 0.0001 0.0001 0.0002
Bud Site 0.0171 0.0161 0.0022 0.0006 0.0027 0.0007 0.0091 0.0006 0.0031 0.0004 0.0022 0.0004 0.0006 0.0046 0.0012 0.0004 0.0009 0.0001
Cell Periphery 0.0016 0.0007 0.0005 0.0002 0.0005 0.0002 0.0009 0.0001 0.0001 0 0.0002 0.0001 0.0002 0.0005 0.0004 0.0001 0 0.0002
Cytoplasm 0.1604 0.26 0.2311 0.1084 0.1901 0.2421 0.201 0.37 0.3298 0.1999 0.2764 0.2627 0.2206 0.1931 0.2055 0.1873 0.356 0.2292
Cytoplasmic Foci 0.1957 0.2143 0.1907 0.2413 0.1752 0.2843 0.1752 0.1749 0.1783 0.1995 0.2203 0.2615 0.1585 0.2862 0.1464 0.2402 0.1202 0.2017
Eisosomes 0.0014 0.0001 0.0002 0.0001 0.0004 0.0001 0.0004 0 0.0001 0 0.0006 0 0.0002 0.0001 0.0004 0 0.0001 0.0001
Endoplasmic Reticulum 0.0116 0.0055 0.0085 0.0019 0.0069 0.0056 0.0076 0.0037 0.0051 0.0028 0.0031 0.006 0.0056 0.0032 0.0034 0.0038 0.0017 0.0038
Endosome 0.2277 0.2934 0.2477 0.4636 0.3149 0.275 0.2439 0.2214 0.2715 0.3904 0.2719 0.3342 0.1823 0.1661 0.1333 0.3319 0.1749 0.2322
Golgi 0.0264 0.0155 0.0223 0.0259 0.0492 0.0149 0.0177 0.0096 0.0168 0.0311 0.0338 0.0188 0.0074 0.0078 0.0065 0.0121 0.0048 0.0098
Lipid Particles 0.0555 0.0251 0.0176 0.0123 0.0432 0.0297 0.0132 0.003 0.0117 0.0318 0.0629 0.0064 0.0229 0.0043 0.0113 0.0044 0.0109 0.0246
Mitochondria 0.0368 0.0028 0.0157 0.0484 0.0559 0.006 0.023 0.0066 0.0087 0.0045 0.0399 0.0045 0.0015 0.001 0.0032 0.0017 0.0003 0.0076
None 0.1511 0.1224 0.2269 0.0514 0.0919 0.0998 0.2345 0.196 0.1242 0.0976 0.0237 0.0759 0.3575 0.3134 0.4548 0.1991 0.2639 0.271
Nuclear Periphery 0.0114 0.0011 0.0017 0.0015 0.0038 0.0019 0.0032 0.0006 0.0017 0.0017 0.0046 0.002 0.0022 0.0006 0.0009 0.002 0.001 0.0007
Nucleolus 0.0013 0.0003 0.0003 0.0005 0.001 0.0002 0.0016 0.0001 0.0002 0.0001 0.0004 0.0001 0.0005 0.0005 0.0003 0.0002 0.0002 0.0002
Nucleus 0.0048 0.0012 0.0009 0.0013 0.0012 0.001 0.0017 0.0005 0.0009 0.0005 0.0008 0.0007 0.0011 0.0009 0.0007 0.0012 0.0004 0.0004
Peroxisomes 0.0191 0.0048 0.0104 0.0027 0.0104 0.0175 0.021 0.0016 0.0122 0.0151 0.0147 0.0077 0.0198 0.0063 0.014 0.0035 0.0257 0.009
Punctate Nuclear 0.0108 0.0018 0.0018 0.0009 0.0055 0.0028 0.0033 0.0005 0.0014 0.0009 0.0017 0.0012 0.0061 0.0019 0.003 0.001 0.0269 0.0012
Vacuole 0.0103 0.0146 0.0091 0.0301 0.0106 0.0092 0.0092 0.0081 0.0077 0.007 0.0054 0.0066 0.0071 0.0074 0.0054 0.0088 0.0033 0.0048
Vacuole Periphery 0.0027 0.002 0.0019 0.0062 0.0037 0.002 0.0022 0.001 0.0019 0.0024 0.0037 0.0019 0.0011 0.0008 0.001 0.002 0.0006 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.3321 7.803 5.2638 11.0502 6.6875 6.7413 10.669 13.0704 11.1816 12.3189
Translational Efficiency 0.5244 0.4976 0.7183 0.3956 0.6557 0.9738 0.6503 0.4658 0.6307 0.5602

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1148 828 859 129 1552 1446 211 1458 2700 2274 1070 1587

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 696.55 725.05 1016.71 906.40 669.63 711.52 943.92 721.22 681.08 716.45 1002.36 736.27
Standard Deviation 76.69 119.65 132.61 92.35 99.03 101.58 113.90 99.00 91.18 108.70 132.34 110.72
Intensity Change Log 2 — 0.057854 0.545609 0.379921 — 0.087540 0.495300 0.107075 — 0.072481 0.521170 0.252616

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000332 0.000428 0.000724 0.001527 0.001322 0.001040 0.001653 0.000376 0.000901 0.000817 0.000907 0.000469
Bud Neck 0.034005 0.019087 0.015955 0.011190 0.017850 0.021472 0.008129 0.019952 0.024719 0.020604 0.014412 0.019239
Bud Site 0.010828 0.011955 0.022977 0.026185 0.007029 0.013760 0.015516 0.013196 0.008644 0.013103 0.021506 0.014252
Cell Periphery 0.000125 0.000088 0.000119 0.000063 0.000066 0.000069 0.000163 0.000115 0.000091 0.000076 0.000127 0.000111
Cytoplasm 0.183105 0.153116 0.166891 0.035858 0.095417 0.180054 0.153934 0.449361 0.132701 0.170245 0.164336 0.415749
Cytoplasmic Foci 0.357899 0.352091 0.144952 0.196031 0.381784 0.368052 0.143198 0.128090 0.371628 0.362240 0.144606 0.133613
Eisosomes 0.000236 0.000122 0.000078 0.000043 0.000190 0.000161 0.000134 0.000081 0.000210 0.000147 0.000089 0.000078
Endoplasmic Reticulum 0.001362 0.001279 0.003037 0.000736 0.000807 0.000714 0.003980 0.000941 0.001043 0.000920 0.003223 0.000924
Endosome 0.212685 0.233975 0.149817 0.351264 0.288184 0.191864 0.112260 0.022944 0.256083 0.207197 0.142411 0.049632
Golgi 0.020578 0.022104 0.003257 0.008920 0.052254 0.021689 0.001454 0.001142 0.038786 0.021841 0.002901 0.001774
Lipid Particles 0.020160 0.022178 0.015815 0.006356 0.034475 0.020977 0.016671 0.004228 0.028388 0.021414 0.015984 0.004401
Mitochondria 0.008214 0.011005 0.000830 0.001497 0.011961 0.008564 0.000335 0.001486 0.010368 0.009453 0.000732 0.001487
Mitotic Spindle 0.003052 0.006777 0.053568 0.034237 0.004022 0.008250 0.034316 0.009872 0.003609 0.007714 0.049771 0.011853
None 0.001463 0.001960 0.000717 0.000645 0.001247 0.000893 0.002081 0.002067 0.001339 0.001281 0.000986 0.001951
Nuclear Periphery 0.000184 0.000972 0.002386 0.000521 0.000275 0.000517 0.001587 0.000710 0.000237 0.000683 0.002229 0.000695
Nuclear Periphery Foci 0.000608 0.001871 0.015092 0.003494 0.000554 0.000811 0.012713 0.001094 0.000577 0.001197 0.014623 0.001289
Nucleolus 0.001000 0.001914 0.005350 0.000708 0.001200 0.001289 0.003142 0.000883 0.001115 0.001516 0.004915 0.000869
Nucleus 0.013977 0.014962 0.075875 0.004597 0.010063 0.013669 0.125539 0.131575 0.011727 0.014140 0.085668 0.121254
Peroxisomes 0.020701 0.029198 0.014915 0.108848 0.039336 0.034757 0.035944 0.008633 0.031412 0.032733 0.019062 0.016779
Vacuole 0.104674 0.108816 0.305414 0.205918 0.048763 0.107644 0.326645 0.202699 0.072535 0.108071 0.309601 0.202961
Vacuole Periphery 0.004813 0.006102 0.002232 0.001361 0.003202 0.003754 0.000606 0.000554 0.003887 0.004609 0.001911 0.000619

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.84 -3.31 -2.79 -2.56 -1.66 0.51 -0.30 1.82 3.38 2.79 0.26 -0.08 1.45 2.62 3.67
Bud Neck 5.60 6.75 6.24 1.99 1.36 -1.85 5.58 -0.11 1.74 -5.74 2.64 6.00 3.99 1.73 -1.56
Bud Site -0.65 -4.66 -3.44 -3.26 -1.01 -4.73 -3.25 -3.15 1.37 1.54 -4.03 -6.45 -3.05 0.21 3.94
Cell Periphery 1.98 0.53 3.33 1.50 4.03 -0.37 -5.80 -4.09 -4.37 3.78 1.82 -3.88 -0.56 -2.77 3.76
Cytoplasm 2.81 1.21 13.10 9.71 11.07 -11.52 -3.26 -35.61 -23.66 -17.16 -6.45 -4.25 -29.54 -23.70 -21.53
Cytoplasmic Foci 0.72 27.99 11.24 10.35 -4.19 1.88 21.36 37.73 34.20 0.08 1.71 39.18 42.20 38.04 -0.03
Eisosomes 5.98 8.93 9.82 7.37 3.04 1.97 3.29 8.70 8.30 5.58 5.20 11.10 12.37 9.86 2.15
Endoplasmic Reticulum 0.14 -3.63 0.44 0.55 7.47 0.68 -7.60 -1.22 -2.33 7.41 0.48 -8.86 0.22 -0.59 12.69
Endosome -1.77 10.38 -2.73 -1.97 -6.45 11.28 17.24 38.51 29.23 5.72 7.62 20.38 37.00 29.38 11.47
Golgi -0.47 11.78 7.16 6.84 -3.37 10.78 21.33 21.44 12.69 0.22 8.83 23.40 23.98 16.27 2.13
Lipid Particles -0.73 2.74 5.55 5.23 3.19 5.74 6.77 15.17 11.50 4.81 4.12 7.91 17.57 13.44 8.33
Mitochondria -1.13 5.40 4.26 4.55 -0.40 2.46 10.92 9.71 7.32 -2.72 0.83 11.40 10.26 8.43 -1.51
Mitotic Spindle -1.89 -10.06 -3.24 -2.85 2.05 -2.77 -2.73 -3.95 -1.25 1.87 -3.39 -10.37 -5.26 -2.64 8.09
None -0.73 1.57 1.55 1.98 0.57 0.56 -0.61 -0.74 -1.87 0.22 0.04 0.86 -0.57 -0.67 -1.72
Nuclear Periphery -2.62 -11.92 -3.31 0.23 7.46 -0.93 -9.97 -6.72 -1.58 6.91 -2.27 -13.07 -9.58 -1.16 10.03
Nuclear Periphery Foci -2.04 -8.91 -4.00 -2.12 5.79 -0.62 -6.60 -3.41 -1.32 6.35 -2.07 -10.29 -5.17 -0.44 9.75
Nucleolus -2.72 -4.10 0.44 2.65 4.12 -0.13 -2.26 1.27 1.25 2.67 -1.75 -4.39 1.36 2.85 4.72
Nucleus -0.62 -11.73 3.72 3.66 12.85 -2.33 -7.94 -27.26 -26.53 -1.17 -2.08 -14.46 -26.57 -25.74 -6.05
Peroxisomes -3.10 4.61 -4.70 -4.05 -5.28 1.54 2.59 12.65 11.49 3.37 -0.56 8.26 8.06 8.19 0.27
Vacuole -0.85 -23.24 -6.65 -6.39 1.85 -13.47 -17.64 -28.20 -18.02 7.67 -9.63 -32.12 -25.92 -19.56 9.41
Vacuole Periphery -1.66 6.60 6.28 7.03 1.56 -1.22 9.27 9.09 10.85 0.63 -1.75 8.63 12.66 13.78 6.98
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Ubiquitin hydrolase; deubiquitinates intralumenal vesicle (ILVs) cargo proteins; required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins destined for the vacuole; DOA4 has a paralog, UBP5, that arose from the whole genome duplication
Localization
Cell Percentages endosome (25%), cytoplasm (17%), mixed (23%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Doa4

Doa4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Doa4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available