Standard name
Human Ortholog
Description Subunit of the HOPS endocytic tethering complex; vacuole membrane protein that functions as a Rab GTPase effector, interacting specifically with the GTP-bound conformation of Ypt7p, facilitating tethering, docking and promoting membrane fusion events at the late endosome and vacuole; required for both membrane and protein trafficking; Yck3p-mediated phosphorylation regulates the organization of vacuolar fusion sites
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.07 0.08 0.07 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.05 0.06 0.1 0.1 0.14 0.19 0.21 0.17 0.22 0.23 0.19 0.1 0.16 0.11 0.29 0.15 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.06 0.05 0 0 0 0 0 0 0
Endosome 0.36 0 0.42 0.47 0.57 0.23 0.38 0.24 0.15 0.09 0.08 0.15 0.22 0.2 0.3 0.32 0.32 0.48 0.33 0.35 0.46 0.46 0.48 0.54
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.54 0.22 0.36 0.23 0.55 0.33 0.5 0.61 0.65 0.68 0.59 0 0 0 0.08 0.13 0 0 0 0 0 0 0
Nucleus 0 0.07 0 0 0 0 0 0 0 0 0 0 0.1 0 0 0 0.06 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.07 0 0.1 0.05 0 0
Nucleolus 0.06 0 0.08 0.05 0 0.08 0.06 0.07 0.07 0 0.07 0.08 0.08 0.05 0 0 0.07 0.16 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0.06 0.07 0.06 0.07 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.1 0.14 0.12 0.16 0.17 0.18 0.17 0.19 0.08 0.08 0.11 0 0.09 0.16 0 0 0 0 0 0
Vac/Vac Membrane 0.71 0.62 0.55 0.3 0.29 0.18 0.18 0.1 0.09 0.09 0.09 0.08 0.64 0.7 0.61 0.52 0.54 0.52 0.51 0.53 0.37 0.38 0.38 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 1 0 1 2 1 0 1 1 0 0 0 0 0
Bud 2 4 0 2 8 17 16 39 35 34 36 36 0 0 0 1 0 0 0 1 1 0 0 4
Bud Neck 0 0 0 0 0 0 1 0 0 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 0 0 0 3 0 1 0 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 23 9 18 21 38 59 98 119 71 111 104 120 5 10 20 36 20 2 1 2 2 1 1 3
Endoplasmic Reticulum 1 3 2 4 2 4 3 1 1 3 2 0 2 5 6 8 7 1 0 1 2 1 0 5
Endosome 132 4 127 94 213 97 198 134 63 48 38 93 11 13 57 40 44 12 130 71 147 84 107 143
Golgi 0 0 1 5 0 0 0 0 0 0 0 0 0 0 1 0 2 0 2 0 3 2 0 1
Mitochondria 37 100 68 73 86 229 169 285 261 337 310 365 1 0 0 10 18 1 15 4 6 5 9 7
Nucleus 10 12 3 0 1 3 7 4 1 1 1 3 5 0 8 3 8 0 2 2 0 3 1 1
Nuclear Periphery 4 5 13 5 0 5 7 1 2 2 3 1 3 0 1 3 4 0 28 6 33 8 9 11
Nucleolus 21 7 24 10 10 34 32 37 31 21 31 47 4 3 7 1 9 4 2 0 0 3 0 0
Peroxisomes 2 0 1 1 10 17 31 39 24 36 17 36 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 16 4 7 9 36 58 63 91 73 91 78 121 4 5 21 5 12 4 4 1 3 3 4 3
Vac/Vac Membrane 256 114 168 61 108 74 94 59 39 48 40 47 32 45 115 66 73 13 199 108 119 70 84 76
Unique Cell Count 362 184 305 202 372 418 516 566 428 515 457 621 50 64 190 126 136 25 394 206 323 185 223 265
Labelled Cell Count 504 263 432 286 512 597 719 809 601 738 661 874 67 82 239 175 197 38 394 206 323 185 223 265


Vacuole, Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 4.9 6.1 6.1 5.6 4.8 5.0 4.7 4.3 4.0 4.0 4.3 6.1 6.2 6.5 5.5 6.4 7.6 7.4 7.4 7.6
Std Deviation (1e-4) 1.6 0.6 1.7 1.6 1.5 1.4 1.6 1.2 0.9 0.9 0.7 1.0 0.6 0.6 0.7 1.2 1.2 1.6 1.5 1.4 1.3
Intensity Change (Log2) – – – -0.02 -0.13 -0.36 -0.29 -0.39 -0.51 -0.6 -0.6 -0.53 -0.02 0.02 0.09 -0.15 0.06 0.3 0.26 0.26 0.31

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP70002468WT3HU80HU120HU16002468WT3rpd3Δ_1rpd3Δ_2rpd3Δ_302468WT1AF100AF140AF18002468
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 3.1 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 2.0 3.5 5.2 5.8 4.4 5.9 6.2 5.4 0 0 1.9 6.5 3.0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 1.1 4.0 0 -0.9 0 0 0 0 0 -2.6 -3.2 -2.6 -1.9 -1.8 0.6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.4 0.3 0 3.2 0 0 0 0 0 -3.4 -4.2 -7.0 -3.5 -2.2 -2.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.1 -4.0 0 -2.6 0 0 0 0 0 0 0 -2.4 0 -0.7 0
Nucleolus -1.3 -3.1 0 -0.9 0 0 0 0 0 0 0 -1.9 -2.9 -0.5 0
Peroxisomes 0 0 0 4.1 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1.4 3.9 0 4.9 0 0 0 0 0 0 0 4.1 0 3.1 0
Vacuole -5.5 -6.9 -10.5 -10.9 -14.3 -13.6 -14.4 -14.1 -16.1 1.2 2.2 1.2 -0.5 -0.3 -0.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3991 1.7886 1.8048 1.6988 1.1971 1.7634 2.1573 3.0685 2.364 1.9166 2.1424 2.3611 2.0171 2.3442 2.1395 1.359 2.1252 1.9609
Actin 0.0021 0 0 0.0001 0 0.0004 0.004 0 0.0077 0.0109 0.0031 0.0001 0.0046 0.0001 0.0057 0 0 0.0031
Bud 0.0001 0.0001 0.0002 0.0001 0.0003 0.0005 0.0004 0.0003 0.0006 0.0002 0.001 0.0002 0.0004 0.0006 0.0006 0.0001 0.0001 0.0002
Bud Neck 0.0004 0.0003 0.0003 0.0002 0.0004 0.0005 0.0008 0.0009 0.0005 0.0004 0.0052 0.0006 0.0003 0.0005 0.0013 0.0004 0.0001 0.0006
Bud Periphery 0.0001 0.0001 0.0002 0.0001 0.0002 0.0003 0.0005 0.0004 0.0006 0.0003 0.0011 0.0003 0.0012 0.0003 0.0008 0.0001 0 0.0004
Bud Site 0.0004 0.0003 0.0009 0.0002 0.0005 0.0002 0.0025 0.003 0.0013 0.0004 0.0198 0.0004 0.0007 0.0082 0.0015 0.0001 0.0001 0.0004
Cell Periphery 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0.0015 0.0002 0.0001 0.0004 0.0002 0.0004 0.0004 0.0003 0.0001 0 0.0002
Cytoplasm 0.0143 0.0182 0.0278 0.0039 0.0116 0.0113 0.0302 0.0372 0.0124 0.022 0.0107 0.027 0.0083 0.1442 0.0642 0.0025 0.0128 0.0325
Cytoplasmic Foci 0.0547 0.0372 0.052 0.0312 0.0301 0.0528 0.0593 0.0419 0.0894 0.0383 0.0607 0.0565 0.041 0.0558 0.0747 0.0305 0.0283 0.0419
Eisosomes 0 0 0 0 0 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0041 0.0028 0.0034 0.0007 0.0011 0.0023 0.0061 0.0037 0.0028 0.0011 0.0037 0.0024 0.0161 0.0056 0.0042 0.0006 0.0006 0.004
Endosome 0.6353 0.6716 0.6478 0.7217 0.7446 0.6254 0.4992 0.4279 0.5538 0.5737 0.5807 0.608 0.4575 0.5064 0.4869 0.5972 0.6587 0.5803
Golgi 0.0146 0.0132 0.0211 0.0283 0.0195 0.0216 0.0318 0.0088 0.0384 0.0312 0.0228 0.0284 0.0301 0.0119 0.0229 0.0172 0.0106 0.0262
Lipid Particles 0.0231 0.0074 0.0114 0.0121 0.0024 0.0151 0.053 0.016 0.0432 0.0592 0.0368 0.0376 0.0731 0.0131 0.0333 0.0312 0.0025 0.0129
Mitochondria 0.002 0.0027 0.0128 0.0243 0.0024 0.0095 0.0163 0.0077 0.0334 0.0087 0.002 0.0164 0.0725 0.0029 0.0146 0.0037 0.001 0.0141
None 0.0116 0.0016 0.0051 0.0003 0.0003 0.0088 0.0518 0.0886 0.0261 0.0048 0.0047 0.001 0.0234 0.024 0.0421 0.0001 0.0001 0.0196
Nuclear Periphery 0.0103 0.0038 0.0074 0.0012 0.0015 0.0051 0.0346 0.0064 0.0077 0.0022 0.0044 0.0037 0.0407 0.0051 0.0071 0.0016 0.0018 0.0055
Nucleolus 0.0034 0.0004 0.0006 0.0002 0.0003 0.0005 0.0013 0.005 0.0008 0.001 0.0016 0.0016 0.0008 0.0006 0.0007 0.0005 0.0002 0.0004
Nucleus 0.0027 0.0007 0.0021 0.0003 0.0008 0.0011 0.0108 0.0054 0.001 0.0029 0.0024 0.0015 0.0024 0.0024 0.002 0.0003 0.0004 0.0021
Peroxisomes 0.0036 0.0016 0.0013 0.0027 0.0008 0.0052 0.0271 0.0014 0.0227 0.009 0.0057 0.0036 0.0074 0.0009 0.0163 0.0071 0.0005 0.0024
Punctate Nuclear 0.0033 0.0001 0.0003 0.0001 0.0001 0.0035 0.0053 0.0005 0.0024 0.0009 0.01 0.0007 0.0023 0.0005 0.0017 0.0002 0.0001 0.0011
Vacuole 0.1619 0.1572 0.1386 0.1164 0.1298 0.1413 0.1189 0.2833 0.1131 0.1492 0.159 0.1395 0.1239 0.1736 0.1684 0.1757 0.1864 0.1718
Vacuole Periphery 0.0519 0.0807 0.0667 0.0558 0.0533 0.0945 0.0456 0.0599 0.0416 0.0834 0.0643 0.0703 0.0931 0.0427 0.0508 0.1308 0.0957 0.0803

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.1487 5.96 11.3021 11.73 10.0849 11.1057 10.2109 14.5742 15.1563 13.5481
Translational Efficiency 1.2202 1.2693 0.7564 0.871 0.8894 0.919 1.068 0.5721 0.6463 0.8294

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
552 1927 298 1397 1206 1560 1409 1838 1758 3487 1707 3235

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 864.58 849.74 1026.82 987.66 633.68 847.22 920.15 874.93 706.18 848.61 938.77 923.61
Standard Deviation 117.37 122.49 112.59 133.89 77.04 112.82 121.76 116.61 141.00 118.26 126.85 136.33
Intensity Change Log 2 — -0.024978 0.248112 0.192015 — 0.418982 0.538115 0.465413 — 0.179665 0.377943 0.314022

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001886 0.000027 0.000862 0.000190 0.000235 0.000053 0.000103 0.000063 0.000753 0.000039 0.000236 0.000118
Bud Neck 0.009876 0.002410 0.010404 0.007157 0.010384 0.002739 0.008487 0.012683 0.010224 0.002557 0.008822 0.010296
Bud Site 0.022436 0.007835 0.020248 0.022178 0.021046 0.003992 0.010718 0.010208 0.021482 0.006116 0.012382 0.015377
Cell Periphery 0.000127 0.000066 0.000085 0.000074 0.000845 0.000138 0.000039 0.000101 0.000619 0.000098 0.000047 0.000089
Cytoplasm 0.014296 0.019371 0.006167 0.019080 0.055195 0.004688 0.011708 0.009683 0.042353 0.012802 0.010741 0.013741
Cytoplasmic Foci 0.041731 0.061797 0.089421 0.046285 0.156481 0.033103 0.040503 0.023854 0.120451 0.048960 0.049043 0.033541
Eisosomes 0.000029 0.000009 0.000039 0.000012 0.000276 0.000017 0.000019 0.000028 0.000199 0.000013 0.000023 0.000021
Endoplasmic Reticulum 0.001573 0.000708 0.001605 0.000777 0.008547 0.001743 0.001307 0.001894 0.006357 0.001171 0.001359 0.001412
Endosome 0.296665 0.296693 0.344703 0.249654 0.212280 0.331603 0.299532 0.308865 0.238776 0.312311 0.307417 0.283295
Golgi 0.019435 0.024393 0.042795 0.029134 0.058553 0.020005 0.015346 0.014606 0.046270 0.022430 0.020138 0.020880
Lipid Particles 0.003084 0.001268 0.003184 0.000928 0.007762 0.005658 0.001565 0.003829 0.006293 0.003232 0.001847 0.002576
Mitochondria 0.016173 0.014187 0.005525 0.005649 0.157697 0.023510 0.004896 0.012175 0.113260 0.018358 0.005006 0.009357
Mitotic Spindle 0.008789 0.007989 0.079673 0.019335 0.000565 0.002002 0.020535 0.013896 0.003147 0.005310 0.030859 0.016245
None 0.000189 0.000337 0.000137 0.000116 0.000881 0.000796 0.000185 0.001591 0.000663 0.000542 0.000177 0.000954
Nuclear Periphery 0.002131 0.000675 0.003081 0.000936 0.000247 0.000725 0.002270 0.003695 0.000838 0.000697 0.002411 0.002504
Nuclear Periphery Foci 0.001861 0.001839 0.013547 0.002618 0.000167 0.002691 0.004886 0.005817 0.000699 0.002220 0.006398 0.004436
Nucleolus 0.004120 0.001222 0.007277 0.001544 0.000240 0.001810 0.007820 0.012457 0.001459 0.001485 0.007725 0.007744
Nucleus 0.007677 0.001758 0.007383 0.002449 0.003426 0.001710 0.009421 0.016870 0.004760 0.001737 0.009065 0.010642
Peroxisomes 0.006130 0.002091 0.021942 0.004174 0.004391 0.004123 0.006684 0.004499 0.004937 0.003000 0.009347 0.004359
Vacuole 0.441559 0.342562 0.252679 0.490158 0.125102 0.347221 0.438797 0.433401 0.224467 0.344647 0.406305 0.457911
Vacuole Periphery 0.100234 0.212763 0.089242 0.097552 0.175680 0.211673 0.115180 0.109784 0.151991 0.212276 0.110652 0.104502

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.88 0.94 1.70 -3.71 2.65 5.08 3.21 4.82 -0.76 2.70 2.29 1.57 2.02 -3.91 2.50
Bud Neck 5.49 -0.65 1.45 -7.96 2.35 7.39 1.30 -1.76 -10.44 -3.53 9.32 1.01 -0.37 -13.04 -1.67
Bud Site 4.16 0.18 -0.24 -8.05 -0.48 10.57 5.60 5.88 -6.71 0.52 9.69 4.81 3.01 -9.68 -2.43
Cell Periphery 2.79 1.84 1.75 -1.38 0.08 6.67 7.66 6.86 1.27 -2.31 7.11 7.84 7.04 0.20 -2.92
Cytoplasm -1.42 2.96 -2.60 -1.56 -7.67 14.73 11.24 12.42 -4.23 1.24 11.01 10.72 9.57 -2.34 -2.40
Cytoplasmic Foci -5.26 -6.69 -1.11 5.35 6.49 27.38 25.86 30.65 4.61 8.43 19.88 19.15 25.09 8.63 7.35
Eisosomes 5.70 -2.13 4.38 -2.21 6.34 7.11 7.04 6.80 -5.51 -4.23 7.41 6.95 7.05 -6.90 1.35
Endoplasmic Reticulum 4.00 -0.28 3.22 -0.86 3.08 7.49 8.03 7.43 -0.55 -2.27 8.40 7.88 7.97 -1.71 0.22
Endosome -0.13 -2.30 4.28 6.40 5.93 -12.49 -9.12 -10.79 2.64 -1.37 -10.22 -7.76 -5.67 5.25 3.11
Golgi -1.86 -4.70 -2.77 -1.54 3.07 12.31 14.73 14.70 3.46 0.43 10.26 11.23 11.01 1.63 -0.41
Lipid Particles 1.60 -0.23 1.84 1.34 4.01 1.50 6.47 3.46 1.57 -2.82 3.61 5.67 4.53 1.09 -1.28
Mitochondria 0.66 3.81 3.81 6.58 -0.05 21.74 25.90 24.46 5.44 -6.86 20.97 24.44 23.36 7.56 -5.54
Mitotic Spindle 0.36 -6.38 -3.47 -4.47 5.31 -3.36 -8.16 -7.96 -7.01 2.38 -2.17 -9.60 -8.51 -7.25 4.60
None -1.86 0.94 1.14 2.98 0.37 0.11 1.70 -1.06 -1.05 -2.61 0.31 1.72 -0.72 -1.06 -2.54
Nuclear Periphery 1.59 -0.77 1.19 -2.19 2.63 -4.42 -6.32 -6.22 -5.31 -1.89 0.51 -3.98 -3.91 -5.66 0.05
Nuclear Periphery Foci 0.14 -3.38 -1.08 -1.26 3.08 -3.34 -5.34 -5.75 -2.52 -0.49 -3.37 -5.96 -5.79 -3.08 1.84
Nucleolus 2.85 -2.05 2.43 -0.96 3.97 -3.45 -10.07 -10.79 -8.74 -2.99 -0.12 -8.73 -8.64 -8.92 0.47
Nucleus 3.39 -0.07 2.39 -2.21 2.77 1.49 -4.71 -6.85 -9.02 -3.01 3.21 -4.08 -4.60 -9.21 -0.48
Peroxisomes 4.10 -4.12 1.97 -3.41 4.76 0.20 -1.95 -0.23 -0.45 2.01 2.87 -3.78 0.91 -2.70 4.62
Vacuole 7.86 11.01 -3.79 -17.16 -16.64 -28.08 -39.36 -39.74 -9.89 0.71 -16.50 -21.57 -30.84 -18.68 -6.88
Vacuole Periphery -16.22 1.35 0.90 20.79 -0.84 -4.95 10.11 10.95 17.78 0.85 -11.34 8.61 10.68 26.95 1.52
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Subunit of the HOPS endocytic tethering complex; vacuole membrane protein that functions as a Rab GTPase effector, interacting specifically with the GTP-bound conformation of Ypt7p, facilitating tethering, docking and promoting membrane fusion events at the late endosome and vacuole; required for both membrane and protein trafficking; Yck3p-mediated phosphorylation regulates the organization of vacuolar fusion sites
Localization
Cell Percentages vacuolar membrane (36%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-1

Vps41

Vps41


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vps41-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available